Improved media for growth and sporulation of Sporidesmium sclerotivorum

1983 ◽  
Vol 29 (3) ◽  
pp. 325-330 ◽  
Author(s):  
William A. Ayers ◽  
Peter B. Adams

Additional nutritional and environmental factors affecting growth and sporulation of the beneficial mycoparasite, Sporidesmium sclerotivorum, were determined. A liquid medium composed of mineral salts, glucose, glutamine, thiamine, biotin, and succinic acid supported good growth and sporulation of isolate CS-5 within 5 weeks at 25 °C. Growth was substantially greater in a medium with KH2PO4, CaCl2, minor elements, and ferric–potassium salt of EDTA (FeKEDTA) than in media with single omissions of these ingredients. Iron supplied as FeCl3 or FeKEDTA at 10−5 M or greater markedly stimulated production of conidia. Supplementation of the medium with Casamino acids or with Soytone and yeast extract decreased the lag phase slightly but did not affect the maximum growth rate nor final mycelial and spore yield. Succinic acid (0.2%) buffered the medium against a rapid drop in pH and thereby promoted growth. The growth rate was independent of glucose concentrations of 0.5 to 2%, but the total mycelial yield was dependent upon the total amount of glucose furnished. Conidia developed primarily on mycelial mat surfaces, and maximum spore yields were favored by cultivation in shallow depths of medium.

2013 ◽  
Vol 76 (11) ◽  
pp. 1963-1968 ◽  
Author(s):  
QIANWANG ZHENG ◽  
CAROLINE BUSTANDI ◽  
YISHAN YANG ◽  
KEITH R. SCHNEIDER ◽  
HYUN-GYUN YUK

This study was performed to optimize Salmonella Typhimurium recovery from raw duck wings with five nonselective broths (buffered peptone water, tryptic soy broth, lactose broth, universal preenrichment broth, nutrient broth) and four selective broths (selenite broth, BAX System MP media [MP], Salmonella AD media [AD], ONE broth-Salmonella [OB]). Healthy or heat-injured (50 and 85% injury) cells were inoculated at a level of 102, 101, or 100 CFU/25 g on raw duck wings. Growth was modeled using DMfit with four growth parameters: lag-phase duration, maximum growth rate, doubling time, and maximum population density. Most enrichments were able to recover Salmonella Typhimurium to greater than 6 log CFU/ml. AD, MP, and OB had significantly (P < 0.05) higher maximum growth rate (0.9 to 1.0/h) and lower doubling time (0.7 to 0.8 h). Buffered peptone water, AD, MP, and OB recovered healthy and 50%-injured cells at low inoculum levels to more than 6.0 log CFU/ml; OB achieved the greatest recovery (7.6 and 7.9 log CFU/ml), following 24 h of incubation. The 85%-injured cells at 100 and 101 CFU/25 g, however, were only recovered in OB, reaching 7.3 and 7.5 log CFU/ml, respectively. These results suggest that OB may be an appropriate enrichment broth for the recovery of Salmonella Typhimurium from raw duck wings in standard diagnostic tests or other rapid detection methods, to avoid false-negative results.


Author(s):  
SUBASH SHRESTHA ◽  
Jerry Joseph Erdmann ◽  
Sean A Smith

The use of antimicrobials in formulations of ready-to-eat meat and poultry products has been identified as a major strategy to control Listeria monocytogenes . The USDA-FSIS recommends no more than 2-logs of Listeria outgrowth over the stated shelf life if antimicrobials are used as a control measure for a product with post-lethality environmental exposure. This study was designed to understand the efficacy of a clean label antimicrobial against the growth of L. monocytogenes as affected by the product attributes. A response surface method-central composite design was used to investigate the effects of product pH, moisture, salt content, and a commercial “clean-label” antimicrobial on the growth of L. monocytogenes in a model turkey deli meat formulation. Thirty treatment combinations of pH (6.3, 6.5, and 6.7), moisture (72, 75, and 78%), salt (1.0, 1.5, and 2.0%), and antimicrobial (0.75, 1.375, and 2.0%) with six replicated center points and eight design star points were evaluated. Treatments were surface inoculated with a 3 log 10 CFU/g target of a five-strain L. monocytogenes cocktail, vacuum packaged, and stored at 5°C for up to 16 weeks. Populations of L. monocytogenes were enumerated from triplicate samples every week until the stationary growth phase was reached. The enumeration data was fitted to a Baranyi and Roberts growth curve to calculate the lag time and maximum growth rate for each treatment.  Linear least-squares regression of the lag-time and growth-rate against the full quadratic, including the second order interaction terms, design matrix was performed. Both lag time and maximum growth rate were significantly affected ( p <0.01) by the antimicrobial concentration and product pH. Product moisture and salt content affected ( p <0.05) lag phase and maximum growth rate, respectively. The availability of a validated growth model assists meat scientists and processors with faster product development and commercialization.


1993 ◽  
Vol 57 (2) ◽  
pp. 332-334 ◽  
Author(s):  
A. Blasco ◽  
E. Gómez

Two synthetic lines of rabbits were used in the experiment. Line V, selected on litter size, and line R, selected on growth rate. Ninety-six animals were randomly collected from 48 litters, taking a male and a female each time. Richards and Gompertz growth curves were fitted. Sexual dimorphism appeared in the line V but not in the R. Values for b and k were similar in all curves. Maximum growth rate took place in weeks 7 to 8. A break due to weaning could be observed in weeks 4 to 5. Although there is a remarkable similarity of the values of all the parameters using data from the first 20 weeks only, the higher standard errors on adult weight would make 30 weeks the preferable time to take data for live-weight growth curves.


1978 ◽  
Vol 14 (1) ◽  
pp. 1-5 ◽  
Author(s):  
J. L. Monteith

SUMMARYFigures for maximum crop growth rates, reviewed by Gifford (1974), suggest that the productivity of C3 and C4 species is almost indistinguishable. However, close inspection of these figures at source and correspondence with several authors revealed a number of errors. When all unreliable figures were discarded, the maximum growth rate for C3 stands fell in the range 34–39 g m−2 d−1 compared with 50–54 g m−2 d−1 for C4 stands. Maximum growth rates averaged over the whole growing season showed a similar difference: 13 g m−2 d−1 for C3 and 22 g m−2 d−1 for C4. These figures correspond to photosynthetic efficiencies of approximately 1·4 and 2·0%.


1992 ◽  
Vol 25 (6) ◽  
pp. 167-183 ◽  
Author(s):  
H. Siegrist ◽  
M. Tschui

The wastewater of the municipal treatment plants Zürich-Werdhölzli (350000 population equivalents), Zürich-Glatt (110000), and Wattwil (20000) have been characterized with regard to the activated sludge model Nr.1 of the IAWPRC task group. Zürich-Glatt and Wattwil are partly nitrifying treatment plants and Zürich-Werdhölzli is fully nitrifying. The mixing characteristics of the aeration tanks at Werdhölzli and Glatt were determined with sodium bromide as a tracer. The experimental data were used to calibrate hydrolysis, heterotrophic growth and nitrification. Problems arising by calibrating hydrolysis of the paniculate material and by measuring oxygen consumption of heterotrophic and nitrifying microorganisms are discussed. For hydrolysis the experimental data indicate first-order kinetics. For nitrification a maximum growth rate of 0.40±0.07 d−1, corresponding to an observed growth rate of 0.26±0.04 d−1 was calculated at 10°C. The half velocity constant found for 12 and 20°C was 2 mg NH4-N/l. The calibrated model was verified with experimental dam of me Zürich-Werdhölzli treatment plant during ammonia shock load.


1987 ◽  
Vol 44 (11) ◽  
pp. 1995-2001 ◽  
Author(s):  
Stephen H. Bowen

It is widely believed that fishes require more dietary protein than other vertebrates. Many aspects of fish physiology, nutrition, and trophic ecology have been interpreted within the context of this high protein requirement. Here, fishes are compared with terrestrial homeotherms in terms of (1) protein requirement for maintenance, (2) relative protein concentration in the diet required for maximum growth rate, (3) protein intake rate required for maximum growth rate, (4) efficiency of protein retention in growth, and (5) weight of growth achieved per weight of protein ingested. The two animal groups compared differ only in relative protein concentration in the diet required for maximum growth rate. This difference is explained in terms of homeotherms' greater requirement for energy and does not reflect absolute differences in protein requirement. The remaining measures of protein requirement suggest that fishes and terrestrial homeotherms are remarkably similar in their use of protein as a nutritional resource. Reinterpretation of the role of protein in fish physiology, nutrition, and trophic ecology is perhaps in order.


2012 ◽  
Vol 57 (2) ◽  
pp. 554-566 ◽  
Author(s):  
Kyle F. Edwards ◽  
Mridul K. Thomas ◽  
Christopher A. Klausmeier ◽  
Elena Litchman

1993 ◽  
Vol 248 ◽  
pp. 363-381 ◽  
Author(s):  
G. H. Wheless ◽  
G. T. Csanady

We used a compound matrix method to integrate the Orr–Sommerfeld equation in an investigation of short instability waves (λ < 6 cm) on the coupled shear flow at the air–sea interface under suddenly imposed wind (a gust model). The method is robust and fast, so that the effects of external variables on growth rate could easily be explored. As expected from past theoretical studies, the growth rate proved sensitive to air and water viscosity, and to the curvature of the air velocity profile very close to the interface. Surface tension had less influence, growth rate increasing somewhat with decreasing surface tension. Maximum growth rate and minimum wave speed nearly coincided for some combinations of fluid properties, but not for others.The most important new finding is that, contrary to some past order of magnitude estimates made on theoretical grounds, the eigenfunctions at these short wavelengths are confined to a distance of the order of the viscous wave boundary-layer thickness from the interface. Correspondingly, the perturbation vorticity is high, the streamwise surface velocity perturbation in typical cases being five times the orbital velocity of free waves on an undisturbed water surface. The instability waves should therefore be thought of as fundamentally different flow structures from free waves: given their high vorticity, they are akin to incipient turbulent eddies. They may also be expected to break at a much lower steepness than free waves.


2019 ◽  
Vol 9 (1) ◽  
pp. 14-21
Author(s):  
Kiran P. Savanur

This article examines the research output of economics published by BRICS countries during 1991-2016. Data collected from the Web of Science database. Growth rate (CAGR), Collaboration index, Transformative Activity index (TAI), Co-authorship index and Relative Citation Impact (RCI) indicators have been adopted to analyze the quantity and impact of economic research. We found that all five BRICS countries contributed approximately 10 percentile of the world’s economics research. The highest contribution was made by China with a total of 4424 articles which is 40.59 percent. Russia has the maximum growth rate of 27.99. Overall collaboration rate of economics publications of BRICS countries is moderate.


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