scholarly journals Effects of Wood Vinegar and Bio char on Germination of Pakchoi Seeds under Different Cadmium Stress Conditions

Author(s):  
YongChol Ju ◽  
Xu Zhang ◽  
Chol Jong ◽  
TaeHo Yun ◽  
IINam Ri ◽  
...  

Wood vinegar is widely used as a strong antioxidant, bacteria prevention, plant growth agent, an insecticide, and its effectiveness is shown in heavy metal treatment at this time.Wood vinegar liquid contains organic acids and phenols, which are effective in adsorbing heavy metals. Although a lot of studies have been conducted on the adsorption of heavy metals from biochar, the effect of mixing biochar and wood vinegar liquid on plant budding, and soil heavy metal morphology changes few studies have been analyzed. This paper analyzes the effects of Wood vinegar and biochar on the sprouting of pakchoi grown in different threats of cadmium from the nature of Wood vinegar. As a result, it was confirmed that the optimum concentration of the applied fertilizer wood vinegar that lowers the plant effectiveness of Cadmium was 1.0%. The fresh weight of pakchoi changed significantly in the order of biochar + wood vinegar 1.0% mixing> biochar> control. When 5.0% Biochar was mixed with 1.0% wood vinegar, the immobilization effect of the residual state and the carbonate bound cadmium in the soil was the highest. The combined application of wood vinegar and biochar promotes the germination of pakchoi, and has a significant inactivation effect on cadmium-contaminated soil; the results of analyzing the effectiveness of the mixing of wood vinegar and biochar and separate fertilization for each soil index show that, Compared to before sowing the pH ratio of the mixed treatment of biochar + wood vinegar is higher than that of the single treatment zone, which is as high as between 6.6-6.8, the EC is reduced to 2-59mS/cm width, and the CEC is increased by 0.27-2.21 times. It shows that under heavy metal stress, the mixed treatment of biochar+wood vinegar solution 1.0% is more effective than the treatment of biochar alone and the control.

2021 ◽  
Author(s):  
Dandan Li ◽  
Guandi He ◽  
Weijun Tian ◽  
Yun Huang ◽  
Lulu Meng ◽  
...  

Abstract Metal tolerance proteins play an important role in the transport and tolerance of divalent heavy metals in plant species. Potatoes are an important food crop whose yields can be deeply affected by heavy metals. However, there is a lack of information concerning the members and function of the MTP gene family in Solanum tuberosum. In this study, we identified and screened 11 MTP genes in potatoes which we named as StMTP1 to StMTP11 based on their positions on the chromosomes. Phylogenetic analysis divided these 11 MTP genes into three subfamilies; Mn-MTP, Zn-MTP and Zn/Fe-MTP. HXXXD and DXXXD conserved motifs were found on or around the transmembrane domain II and transmembrane domain V of these proteins. The highly conserved histidine and aspartic acid residues may be related to the transport of metal ions. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression levels of StMTP9 and StMTP10 in leaf tissues increased by around 24-fold following cadmium stress for 24 hours. We hypothesize that StMTP9 and StMTP10 respond to cadmium stress. StMTP11 showed the highest level of expression in stem tissues after 6 hours of zinc stress at more than 13 times the level of expression in controls indicating that StMTP11 is more sensitive to zinc stress. In summary, our results further the current understanding of the molecular mechanisms regulated by members of the MTP gene family in plant responses to heavy metal stress.


Author(s):  
YongChol Ju ◽  
HongYan Wang ◽  
Minchol RI ◽  
KyongYok Sim ◽  
TongSop Ri ◽  
...  

Because of its characteristics, wood vinegar is widely used in agricultural production processes. Wood vinegar can be used as a strong antioxidant, anti-bacteria, plant growth agent, an insecticide, and currently shows superiority in the treatment of heavy metals. Wood vinegar contains organic acids, organic compounds, and phenol, which can effectively adsorb heavy metals. A large number of studies have been conducted on the adsorption of heavy metals by biochar, but few studies have analyzed the effects of biochar and wood vinegar fertilization on the growth of cadmium soil genus plants and changes in soil heavy metal forms. This article analyzed the effects of wood vinegar and biochar mixed fertilizer on the growth and plant efficacy of pakchoi from the properties of wood vinegar, and confirmed that 0.75% wood vinegar liquid treatment is the most effective concentration. By analyzing the effectiveness of 0.75% wood vinegar and biochar mixture and single fertilization, the combined application of biochar + wood vinegar reduced the absorption rate of cadmium by pakchoi leaves and roots by 12.8% and 13.1% compared with the single treatment group. The yield of crops increased by 111.9~150.1%. The results of evaluating the enrichment coefficient and single-factor index are 0.17~0.67%, 0.005-0.008, and the ranking is D> E> C> B> A> CK. When observing the changes in soil morphology, the content of residual cadmium, carbonate-bound cadmium, and iron-manganese oxide-bound cadmium increased by 0.3~233.7 times, and the exchangeable cadmium content and the content of organic and sulfide-bound cadmium were reduced to 1.0~6.6 times. It can be seen that under different cadmium stress conditions, the mixed fertilization of wood vinegar and biochar will reduce the growth of pakchoi and the plant availability of cadmium, and it has a higher mitigation effect on cadmium soil pollution.


2020 ◽  
Vol 1 (1) ◽  
pp. 1-13
Author(s):  
Kanita Šabanović ◽  
Ahmet Yildirim ◽  
Jasmin Šutković

When a plant is under heavy metals stress, it has different mechanism of coping with it. Brassica oleracea var. acephala (kale) is a plant that has an ability of heavy metal accumulation and removal of heavy metals from the ground. The plants were  exposed to 50, 100, 200, and 500 μM of CdCl2 for 5days, in controlled in vitro conditions. Root length was measured to confirm the Cd effect on plant growth. There are five key antioxidants enzymes responsible for the regulation of heavy metals stress: superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), Peroxidase (POD) and Polyphenol oxidase (PPO). All enzymes showed significant activity, especially triggered by 500 μM CdCl2 in both varieties. The domestic sorts seem more resistant if compared to hybrid variety, showing significant lower expression of antioxidants enzymes at higher concentrations.  In general, significant percentage of enzymes is more expressed in the hybrid Italian sort, Nero di Toscana, indicating the ability of domestic sorts to be more resistant to heavy metal stress.


Author(s):  
Sangeetha Annam ◽  
Anshu Singla

Abstract: Soil is a major and important natural resource, which not only supports human life but also furnish commodities for ecological and economic growth. Ecological risk has posed a serious threat to the ecosystem by the degradation of soil. The high-stress level of heavy metals like chromium, copper, cadmium, etc. produce ecological risks which include: decrease in the fertility of the soil; reduction in crop yield & degradation of metabolism of living beings, and hence ecological health. The ecological risk associated, demands the assessment of heavy metal stress levels in soils. As the rate of stress level of heavy metals is exponentially increasing in recent times, it is apparent to assess or predict heavy metal contamination in soil. The assessment will help the concerned authorities to take corrective as well as preventive measures to enhance the ecological and hence economic growth. This study reviews the efficient assessment models to predict soil heavy metal contamination.


Forests ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1608
Author(s):  
Aslihan Esringü ◽  
Metin Turan ◽  
Asli Cangönül

Heavy metal pollution is among the important environmental problems in the world. Many techniques have already been used to remove the heavy metals such as lead (Pb) and cadmium (Cd). Among them, the phytoremediation method is an environmentally friendly and green technology. This study was carried out to determine the efficiency of fulvic acid (FA) application in removing Pb and Cd from polluted soil using Tagetes eracta L. and Zinnia elegans Jacq. ornamental plants. The results indicated that, FA application, number of flower per plants, and plant fresh weight of Tagetes eracta plants and Zinnia elegans plants increased 187.5%, 104.5% and 155.5%, 57.7%, respectively with application of 7000 mg L−1 FA at 100 mg kg−1 Pb pollution condition, whereas 42.85%, 16.5%, and 44.4–36.1% with application of 7000 mg L−1 FA at 30 mg kg±1 Cd pollution condition, respectively. With the FA application in the Zinnia elegans plant, the root part has accumulated 51.53% more Pb than the shoot part. For Cd, the shoot part accumulated 35.33% more Cd than the root. The effect of FA application on superoxide dismutase (SOD), peroxidase (POD) and, catalase (CAT) of the Tagetes eracta were decreased as 32.7%, 33.1%, and 35.1% for Pb, 21.2%, 25.1%, and 26,1%, for Cd, and 15.1%, 22.7%, and 37.7% for Pb, and 7.55%, 18.0%, and 18.8% for Cd were in Zinnia elegans respectively. In conclusion, Tagetes eracta and Zinnia elegans can not be recommended for remediation of Pb and Cd polluted area, but FA can be recommended for Pb and Cd stabilization in polluted soil.


2019 ◽  
Vol 70 (17) ◽  
pp. 4477-4488 ◽  
Author(s):  
Laura C Terrón-Camero ◽  
M Ángeles Peláez-Vico ◽  
Coral Del-Val ◽  
Luisa M Sandalio ◽  
María C Romero-Puertas

Abstract Anthropogenic activities, such as industrial processes, mining, and agriculture, lead to an increase in heavy metal concentrations in soil, water, and air. Given their stability in the environment, heavy metals are difficult to eliminate and can constitute a human health risk by entering the food chain through uptake by crop plants. An excess of heavy metals is toxic for plants, which have various mechanisms to prevent their accumulation. However, once metals enter the plant, oxidative damage sometimes occurs, which can lead to plant death. Initial production of nitric oxide (NO), which may play a role in plant perception, signalling, and stress acclimation, has been shown to protect against heavy metals. Very little is known about NO-dependent mechanisms downstream from signalling pathways in plant responses to heavy metal stress. In this review, using bioinformatic techniques, we analyse studies of the involvement of NO in plant responses to heavy metal stress, its possible role as a cytoprotective molecule, and its relationship with reactive oxygen species. Some conclusions are drawn and future research perspectives are outlined to further elucidate the signalling mechanisms underlying the role of NO in plant responses to heavy metal stress.


2020 ◽  
Vol 21 (21) ◽  
pp. 8375
Author(s):  
Joanna Gajewska ◽  
Nur Afifah Azzahra ◽  
Özgün Ali Bingöl ◽  
Karolina Izbiańska-Jankowska ◽  
Tomasz Jelonek ◽  
...  

Heavy metal pollution causes many soils to become a toxic environment not only for plants, but also microorganisms; however, little is known how heavy metal contaminated environment affects metabolism of phytopathogens and their capability of infecting host plants. In this study the oomycete Phytophthora infestans (Mont.) de Bary, the most harmful pathogen of potato, growing under moderate cadmium stress (Cd, 5 mg/L) showed nitro-oxidative imbalance associated with an enhanced antioxidant response. Cadmium notably elevated the level of nitric oxide, superoxide and peroxynitrite that stimulated nitrative modifications within the RNA and DNA pools in the phytopathogen structures. In contrast, the protein pool undergoing nitration was diminished confirming that protein tyrosine nitration is a flexible element of the oomycete adaptive strategy to heavy metal stress. Finally, to verify whether Cd is able to modify P. infestans pathogenicity, a disease index and molecular assessment of disease progress were analysed indicating that Cd stress enhanced aggressiveness of vr P. infestans towards various potato cultivars. Taken together, Cd not only affected hyphal growth rate and caused biochemical changes in P. infestans structures, but accelerated the pathogenicity as well. The nitro-oxidative homeostasis imbalance underlies the phytopathogen adaptive strategy and survival in the heavy metal contaminated environment.


2015 ◽  
Vol 1130 ◽  
pp. 263-267 ◽  
Author(s):  
Hee Chan Jang ◽  
Marjorie Valix

In this study, the adaptation of A. thiooxidans to heavy metals leached from saprolitic Ni laterite ores was performed by gradual acclimatisation. The bacteria was cultivated in heavy metals (Ni, Co, Fe, Mg, Cr and Mn) with total concentrations of 2400 to 24000 ppm equivalent to total dissolution of 1 to 10% (w/v) pulp densities of the saprolitic Ni laterite ore. Adaptation evolution mapped from its tolerance index was found to be dependent on metal concentration, acid generation, and period of adaptation. Bio-stimulation of cell growth and acid production was promoted by heavy metal stress on the bacteria. Pre-established heavy metal tolerance of the bacteria improved the leaching rate in its early phase; 20% and 7% increase in Ni and Co metal recoveries were observed in using adapted bacteria. However heavy metal tolerance was also achieved by the bacteria during the leaching process, albeit delayed by a lag phase. These results confirm the robust nature and suitability of A. thiooxidans in direct biomining of Ni ores.


2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Abolghassem Emamverdian ◽  
Yulong Ding ◽  
Yinfeng Xie ◽  
Sirous Sangari

The increased contaminants caused by anthropogenic activities in the environment and the importance of finding pathways to reduce pollution caused the silicon application to be considered an important detoxification agent. Silicon, as a beneficial element, plays an important role in amelioration of abiotic stress, such as an extreme dose of heavy metal in plants. There are several mechanisms involved in silicon mediation in plants, including the reduction of heavy metal uptake by plants, changing pH value, formation of Si heavy metals, and stimulation of enzyme activity, which can work by chemical and physical pathways. The aim of this paper is to investigate the major silicon-related mechanisms that reduce the toxicity of heavy metals in plants and then to assess the role of silicon in increasing the antioxidant enzyme and nonenzyme activities to protect the plant cell.


2016 ◽  
Vol 24 (1) ◽  
pp. 39-51 ◽  
Author(s):  
Anamika Kushwaha ◽  
Radha Rani ◽  
Sanjay Kumar ◽  
Aishvarya Gautam

Heavy metals, such as cobalt, copper, manganese, molybdenum, and zinc, are essential in trace amounts for growth by plants and other living organisms. However, in excessive amounts these heavy metals have deleterious effects. Like other organisms, plants possess a variety of detoxification mechanisms to counter the harmful effects of heavy metals. These include the restriction of heavy metals by mycorrhizal association, binding with plant cell wall and root excretions, metal efflux from the plasma membrane, metal chelation by phytochelatins and metallothioneins, and compartmentalization within the vacuole. Phytoremediation is an emerging technology that uses plants and their associated rhizospheric microorganisms to remove pollutants from contaminated sites. This technology is inexpensive, efficient, and ecofriendly. This review focuses on potential cellular and molecular adaptations by plants that are necessary to tolerate heavy metal stress.


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