scholarly journals Inactivating Gene Expression with Antisense Modified Oligonucleotides

Acta Naturae ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 101-105
Author(s):  
Sidney Altman ◽  
Carlos Angele-Martinez
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Plasmodium Falciparum ◽  
Human Tissue ◽  
Target Gene ◽  
Modified Oligonucleotides ◽  
Modified Nucleotides ◽  
Culture Cells ◽  
Tissue Culture Cells

Modified nucleotides, including phosphoramidates and mesyl nucleotides, are very effective in inactivating gene expression in bacteria. Gyr A is the target gene in several organisms, including Plasmodium falciparum. Antisense reactions with bacteria infecting citrus plants are promising but incomplete. Human tissue culture cells assayed with a different target are also susceptible to the presence of mesyl oligonucleotides.

scholarly journals Small Hairpin RNAs Efficiently Inhibit Hepatitis C IRES–Mediated Gene Expression in Human Tissue Culture Cells and a Mouse Model

2005 ◽  
Vol 12 (3) ◽  
pp. 562-568 ◽  
Author(s):  
Qian Wang ◽  
Christopher H. Contag ◽  
Heini Ilves ◽  
Brian H. Johnston ◽  
Roger L. Kaspar
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Hepatitis C ◽  
Mouse Model ◽  
Human Tissue ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells

1972 ◽  
Vol 69 (2) ◽  
pp. 452-455 ◽  
Author(s):  
B. Fridlender ◽  
M. Fry ◽  
A. Bolden ◽  
A. Weissbach
Keyword(s):  
Tissue Culture ◽  
Dna Polymerase ◽  
Human Tissue ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals Salmonella enterica Serovar Typhimurium Requires the Lpf, Pef, and Tafi Fimbriae for Biofilm Formation on HEp-2 Tissue Culture Cells and Chicken Intestinal Epithelium

2006 ◽  
Vol 74 (6) ◽  
pp. 3156-3169 ◽  
Author(s):  
Nathan A. Ledeboer ◽  
Jonathan G. Frye ◽  
Michael McClelland ◽  
Bradley D. Jones
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Intestinal Epithelium ◽  
Biofilm Formation ◽  
Intestinal Tissue ◽  
Biofilm Growth ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Serovar Typhimurium

ABSTRACT Recent work has demonstrated that Salmonella enterica serovar Typhimurium forms biofilms on HEp-2 tissue culture cells in a type 1 fimbria-dependent manner. To investigate how biofilm growth of HEp-2 tissue culture cells affects gene expression in Salmonella, we compared global gene expression during planktonic growth and biofilm growth. Microarray results indicated that the transcription of ∼100 genes was substantially altered by growth in a biofilm. These genes encode proteins with a wide range of functions, including antibiotic resistance, central metabolism, conjugation, intracellular survival, membrane transport, regulation, and fimbrial biosynthesis. The identification of five fimbrial gene clusters was of particular interest, as we have demonstrated that type 1 fimbriae are required for biofilm formation on HEp-2 cells and murine intestinal epithelium. Mutations in each of these fimbriae were constructed in S. enterica serovar Typhimurium strain BJ2710, and the mutants were found to have various biofilm phenotypes on plastic, HEp-2 cells, and chicken intestinal tissue. The pef and csg mutants were defective for biofilm formation on each of the three surfaces tested, while the lpf mutant exhibited a complete loss of the ability to form a biofilm on chicken intestinal tissue but only an intermediate loss of the ability to form a biofilm on tissue culture cells and plastic surfaces. The bcf mutant displayed increased biofilm formation on both HEp-2 cells and chicken intestinal epithelium, while the sth mutant had no detectable biofilm defects. In all instances, the mutants could be restored to a wild-type phenotype by a plasmid carrying the functional genes. This is the first work to identify the genomic responses of Salmonella to biofilm formation on host cells, and this work highlights the importance of fimbriae in adhering to and adapting to a eukaryotic cell surface. An understanding of these interactions is likely to provide new insights for intervention strategies in Salmonella colonization and infection.

Improvements in the Epstein-Barr-based shuttle vector system for direct cloning in human tissue culture cells

Methods ◽  
1992 ◽  
Vol 4 (2) ◽  
pp. 133-142 ◽  
Author(s):  
Richard A. Swirski ◽  
David Van Den Berg ◽  
Andrew J.M. Murphy ◽  
Claire M. Lambert ◽  
Errol C. Friedberg ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Human Tissue ◽  
Shuttle Vector ◽  
Vector System ◽  
Direct Cloning ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Epstein Barr

Aminoglycoside suppression at UAG, UAA and UGA codons inEscherichia coli and human tissue culture cells

1989 ◽  
Vol 217 (2-3) ◽  
pp. 411-418 ◽  
Author(s):  
Robin Martin ◽  
Anne E. Mogg ◽  
Louise A. Heywood ◽  
Lars Nitschke ◽  
Julian F. Burke
Keyword(s):  
Tissue Culture ◽  
Human Tissue ◽  
Inescherichia Coli ◽  
Culture Cells ◽  
Tissue Culture Cells
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