scholarly journals NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

10.3791/60567 ◽  
2020 ◽  
Author(s):  
Jonathan M. Mendez ◽  
A. Marijke Keestra-Gounder
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Infected Tissue ◽  
Culture Cells ◽  
Tissue Culture Cells

scholarly journals Inactivating Gene Expression with Antisense Modified Oligonucleotides

Acta Naturae ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 101-105
Author(s):  
Sidney Altman ◽  
Carlos Angele-Martinez
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Plasmodium Falciparum ◽  
Human Tissue ◽  
Target Gene ◽  
Modified Oligonucleotides ◽  
Modified Nucleotides ◽  
Culture Cells ◽  
Tissue Culture Cells

Modified nucleotides, including phosphoramidates and mesyl nucleotides, are very effective in inactivating gene expression in bacteria. Gyr A is the target gene in several organisms, including Plasmodium falciparum. Antisense reactions with bacteria infecting citrus plants are promising but incomplete. Human tissue culture cells assayed with a different target are also susceptible to the presence of mesyl oligonucleotides.

Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells

1982 ◽  
Vol 71 (1) ◽  
pp. 1-11 ◽  
Author(s):  
E. Norrby ◽  
S. -N. Chen ◽  
T. Togashi ◽  
H. Shesberadaran ◽  
K. P. Johnson
Keyword(s):  
Tissue Culture ◽  
Measles Virus ◽  
Infected Tissue ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Virus Antigens

scholarly journals Salmonella enterica Serovar Typhimurium Requires the Lpf, Pef, and Tafi Fimbriae for Biofilm Formation on HEp-2 Tissue Culture Cells and Chicken Intestinal Epithelium

2006 ◽  
Vol 74 (6) ◽  
pp. 3156-3169 ◽  
Author(s):  
Nathan A. Ledeboer ◽  
Jonathan G. Frye ◽  
Michael McClelland ◽  
Bradley D. Jones
Keyword(s):  
Gene Expression ◽  
Tissue Culture ◽  
Intestinal Epithelium ◽  
Biofilm Formation ◽  
Intestinal Tissue ◽  
Biofilm Growth ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Serovar Typhimurium

ABSTRACT Recent work has demonstrated that Salmonella enterica serovar Typhimurium forms biofilms on HEp-2 tissue culture cells in a type 1 fimbria-dependent manner. To investigate how biofilm growth of HEp-2 tissue culture cells affects gene expression in Salmonella, we compared global gene expression during planktonic growth and biofilm growth. Microarray results indicated that the transcription of ∼100 genes was substantially altered by growth in a biofilm. These genes encode proteins with a wide range of functions, including antibiotic resistance, central metabolism, conjugation, intracellular survival, membrane transport, regulation, and fimbrial biosynthesis. The identification of five fimbrial gene clusters was of particular interest, as we have demonstrated that type 1 fimbriae are required for biofilm formation on HEp-2 cells and murine intestinal epithelium. Mutations in each of these fimbriae were constructed in S. enterica serovar Typhimurium strain BJ2710, and the mutants were found to have various biofilm phenotypes on plastic, HEp-2 cells, and chicken intestinal tissue. The pef and csg mutants were defective for biofilm formation on each of the three surfaces tested, while the lpf mutant exhibited a complete loss of the ability to form a biofilm on chicken intestinal tissue but only an intermediate loss of the ability to form a biofilm on tissue culture cells and plastic surfaces. The bcf mutant displayed increased biofilm formation on both HEp-2 cells and chicken intestinal epithelium, while the sth mutant had no detectable biofilm defects. In all instances, the mutants could be restored to a wild-type phenotype by a plasmid carrying the functional genes. This is the first work to identify the genomic responses of Salmonella to biofilm formation on host cells, and this work highlights the importance of fimbriae in adhering to and adapting to a eukaryotic cell surface. An understanding of these interactions is likely to provide new insights for intervention strategies in Salmonella colonization and infection.

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