scholarly journals Application of silicon nitride membrane filters in circulating tumor cell capture

2014 ◽  
Vol 17 (2) ◽  
pp. 35-46
Author(s):  
Van Vinh To ◽  
Thi Phuong Tuyen Dao ◽  
Van Binh Pham ◽  
Duy Hien Tong ◽  
Van Hieu Tran
Keyword(s):  
Silicon Nitride ◽  
Tumor Cells ◽  
Early Stage ◽  
Membrane Filter ◽  
Metastatic Cancer ◽  
Circulating Tumor Cell ◽  
Live Cells ◽  
Cell Capture ◽  
Membrane Filters ◽  
Silicon Nitride Membrane

Circulating tumor cells (CTCs) have been recognized as holding extraordinary potential for disease management in cancer patients including prognostic, therapy, and monitoring disease progression. Sensitive and quick detection of CTC could enable the approach to patients with early-stage and metastatic cancer. The technical challenge in this field consists of finding rare tumor cells (just a few CTCs in 1 ml of blood) and being able to distinguish them from epithelial non-tumor cells and leukocytes. The current methodologies have significant limitations such as low capture efficiency, cannot capture live cells and time consuming. This paper presents the development of a new generation of microfilter for size-based isolation of CTCs in epithelial cancer using silicon nitride membrane filters 0.5 cm by 0.5 cm square sheets with slit shaped pores of 5μm by 15μm. We evaluated the sensitivity and efficiency of CTCs capture in a model system using the MCF-7 cells (breast cancer cells) spiked in the blood from the healthy donors. Preliminary results this research shown that silicon nitride membrane filter is a very good candidate to be CTCs detection platfo.

scholarly journals Analysis of the Circulating Tumor Cell Capture Ability of a Slit Filter-Based Method in Comparison to a Selection-Free Method in Multiple Cancer Types

2020 ◽  
Vol 21 (23) ◽  
pp. 9031
Author(s):  
Hidenori Takagi ◽  
Liang Dong ◽  
Morgan D. Kuczler ◽  
Kara Lombardo ◽  
Mitsuharu Hirai ◽  
...  
Keyword(s):  
Metastatic Cancer ◽  
Circulating Tumor Cell ◽  
Cell Capture ◽  
Detection Capability ◽  
Multiple Cancer ◽  
Large Size ◽  
Positive Rate ◽  
Median Diameter ◽  
Cancer Types ◽  
Slit Filter

Circulating tumor cells (CTCs) are a promising biomarker for cancer liquid biopsy. To evaluate the CTC capture bias and detection capability of the slit filter-based CTC isolation platform (CTC-FIND), we prospectively compared it head to head to a selection-free platform (AccuCyte®-CyteFinder® system). We used the two methods to determine the CTC counts, CTC positive rates, CTC size distributions, and CTC phenotypes in 36 patients with metastatic cancer. Between the two methods, the median CTC counts were not significantly different and the total counts were correlated (r = 0.63, p < 0.0001). The CTC positive rate by CTC-FIND was significantly higher than that by AccuCyte®-CyteFinder® system (91.7% vs. 66.7%, p < 0.05). The median diameter of CTCs collected by CTC-FIND was significantly larger (13.0 μm, range 5.2–52.0 vs. 10.4 μm, range 5.2–44.2, p < 0.0001). The distributions of CTC phenotypes (CK+EpCAM+, CK+EpCAM− or CK−EpCAM+) detected by both methods were similar. These results suggested that CTC-FIND can detect more CTC-positive cases but with a bias toward large size of CTCs.

scholarly journals Modification of Hemodialysis Membranes for Efficient Circulating Tumor Cell Capture for Cancer Therapy

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4845
Author(s):  
Gabor Jarvas ◽  
Dora Szerenyi ◽  
Jozsef Tovari ◽  
Laszlo Takacs ◽  
Andras Guttman
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metabolism ◽  
Cell Types ◽  
Circulating Tumor Cell ◽  
Blood Stream ◽  
Normal Function ◽  
Cell Capture ◽  
Buffer Solution ◽  
Cell Clearance

Background: It is well known that more than 90% of cancer deaths are due to metastases. However, the entire tumorigenesis process is not fully understood, and it is evident that cells spreading from the primary tumor play a key role in initiating the metastatic process. Tumor proliferation and invasion also elevate the concentration of regular and irregular metabolites in the serum, which may alter the normal function of the entire human homeostasis and possibly causes cancer metabolism syndrome, also referred to as cachexia. Methods: We report on the modification of commercially available hemodialysis membranes to selectively capture circulating tumor cells from the blood stream by means of immobilized human anti-EpCAM antibodies on the inner surface of the fibers. All critical steps are described that required in situ addition of the immuno-affinity feature to hemodialyzer cartridges in order to capture EpCAM positive circulating tumor cells, which represents ~80% of cancer cell types. Results: The cell capture efficiency of the suggested technology was demonstrated by spiking HCT116 cancer cells both into buffer solution and whole blood and run through on the modified cartridge. Flow cytometry was used to quantitatively evaluate the cell clearance performance of the approach. Conclusions: The suggested modification has no significant effect on the porous structure of the hemodialysis membranes; it keeps its cytokine removal capability, addressing cachexia simultaneously with CTC removal.

High Flow Rate Device for Circulating Tumor Cell Capture

2011 ◽  
Author(s):  
Taehyun Park ◽  
Daniel Sangwon Park ◽  
Michael C. Murphy
Keyword(s):  
Tumor Cells ◽  
Flow Rate ◽  
Recovery Rate ◽  
Circulating Tumor Cell ◽  
High Flow ◽  
High Flow Rate ◽  
Limiting Current ◽  
Target Cells ◽  
Cell Capture ◽  
Cell Sample

Circulating tumor cells (CTCs) were captured at high flow rates with a high recovery rate using a small footprint disposable polymer micro device. A new concept of target cell capture was introduced to break through the barriers limiting current approaches. Several potential designs were parametrically simulated using computational fluid dynamics (CFD) to achieve the best performance. The high flow rate device (HFRD) was fabricated in polymethyl methacrylate (PMMA) based on simulation results. Antibodies (anti-EpCAM) were immobilized on the PMMA device with surface treatments including UV modification and amine functionalization. A novel rare cell sample preparation method was established to provide an exact number of initial target cells to accurately test the rare cell performance. The precisely prepared samples of rare target tumor cells were spiked in a solution containing human erythrocytes, with a 40% hematocrit. The mean recovery rate with the HFRD was 85% at a 750 μL/min flow rate.

Integrated 3D conducting polymer-based bioelectronics for capture and release of circulating tumor cells

2015 ◽  
Vol 3 (25) ◽  
pp. 5103-5110 ◽  
Author(s):  
Yu-Sheng Hsiao ◽  
Bo-Cheng Ho ◽  
Hong-Xin Yan ◽  
Chiung-Wen Kuo ◽  
Di-Yen Chueh ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Conducting Polymer ◽  
Circulating Tumor Cell ◽  
Cell Capture ◽  
Capture And Release

3D conducting polymer-based bioelectronic interface (BEI) devices for dynamically controlling circulating tumor cell capture/release performance through the cyclic potential of electrical stimulation.

Performance and Reliability of a MEMS-based tunable optical filter operating in the 1565 nm-1525 nm wavelength range

2002 ◽  
Vol 722 ◽  
Author(s):  
T. S. Sriram ◽  
B. Strauss ◽  
S. Pappas ◽  
A. Baliga ◽  
A. Jean ◽  
...  
Keyword(s):  
Silicon Nitride ◽  
Line Width ◽  
Insertion Loss ◽  
Optical Filter ◽  
Single Mode ◽  
Single Mode Fiber ◽  
Reliability Testing ◽  
Silicon Nitride Membrane ◽  
Tunable Optical Filter ◽  
Extensive Performance

AbstractThis paper describes the results of extensive performance and reliability characterization of a silicon-based surface micro-machined tunable optical filter. The device comprises a high-finesse Fabry-Perot etalon with one flat and one curved dielectric mirror. The curved mirror is mounted on an electrostatically actuated silicon nitride membrane tethered to the substrate using silicon nitride posts. A voltage applied to the membrane allows the device to be tuned by adjusting the length of the cavity. The device is coupled optically to an input and an output single mode fiber inside a hermetic package. Extensive performance characterization (over operating temperature range) was performed on the packaged device. Parameters characterized included tuning characteristics, insertion loss, filter line-width and side mode suppression ratio. Reliability testing was performed by subjecting the MEMS structure to a very large number of actuations at an elevated temperature both inside the package and on a test board. The MEMS structure was found to be extremely robust, running trillions of actuations without failures. Package level reliability testing conforming to Telcordia standards indicated that key device parameters including insertion loss, filter line-width and tuning characteristics did not change measurably over the duration of the test.

scholarly journals FlowCell-enriched circulating tumor cells as a predictor of lung cancer metastasis

Human Cell ◽  
2021 ◽  
Author(s):  
Yan Lu ◽  
Yushuang Zheng ◽  
Yuhong Wang ◽  
Dongmei Gu ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metastasis ◽  
Lung Tumor ◽  
Early Stage ◽  
High Rate ◽  
Early Stage Lung Cancer ◽  
Lung Cancer Metastasis ◽  
Number Of Patients

AbstractLung cancer is the most fetal malignancy due to the high rate of metastasis and recurrence after treatment. A considerable number of patients with early-stage lung cancer relapse due to overlooked distant metastasis. Circulating tumor cells (CTCs) are tumor cells in blood circulation that originated from primary or metastatic sites, and it has been shown that CTCs are critical for metastasis and prognosis in various type of cancers. Here, we employed novel method to capture, isolate and classify CTC with FlowCell system and analyzed the CTCs from a cohort of 302 individuals. Our results illustrated that FlowCell-enriched CTCs effectively differentiated benign and malignant lung tumor and the total CTC counts increased as the tumor developed. More importantly, we showed that CTCs displayed superior sensitivity and specificity to predict lung cancer metastasis in comparison to conventional circulating biomarkers. Taken together, our data suggested CTCs can be used to assist the diagnosis of lung cancer as well as predict lung cancer metastasis. These findings provide an alternative means to screen early-stage metastasis.

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