scholarly journals Modification of Hemodialysis Membranes for Efficient Circulating Tumor Cell Capture for Cancer Therapy

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4845
Author(s):  
Gabor Jarvas ◽  
Dora Szerenyi ◽  
Jozsef Tovari ◽  
Laszlo Takacs ◽  
Andras Guttman
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Metabolism ◽  
Cell Types ◽  
Circulating Tumor Cell ◽  
Blood Stream ◽  
Normal Function ◽  
Cell Capture ◽  
Buffer Solution ◽  
Cell Clearance

Background: It is well known that more than 90% of cancer deaths are due to metastases. However, the entire tumorigenesis process is not fully understood, and it is evident that cells spreading from the primary tumor play a key role in initiating the metastatic process. Tumor proliferation and invasion also elevate the concentration of regular and irregular metabolites in the serum, which may alter the normal function of the entire human homeostasis and possibly causes cancer metabolism syndrome, also referred to as cachexia. Methods: We report on the modification of commercially available hemodialysis membranes to selectively capture circulating tumor cells from the blood stream by means of immobilized human anti-EpCAM antibodies on the inner surface of the fibers. All critical steps are described that required in situ addition of the immuno-affinity feature to hemodialyzer cartridges in order to capture EpCAM positive circulating tumor cells, which represents ~80% of cancer cell types. Results: The cell capture efficiency of the suggested technology was demonstrated by spiking HCT116 cancer cells both into buffer solution and whole blood and run through on the modified cartridge. Flow cytometry was used to quantitatively evaluate the cell clearance performance of the approach. Conclusions: The suggested modification has no significant effect on the porous structure of the hemodialysis membranes; it keeps its cytokine removal capability, addressing cachexia simultaneously with CTC removal.

Integrated 3D conducting polymer-based bioelectronics for capture and release of circulating tumor cells

2015 ◽  
Vol 3 (25) ◽  
pp. 5103-5110 ◽  
Author(s):  
Yu-Sheng Hsiao ◽  
Bo-Cheng Ho ◽  
Hong-Xin Yan ◽  
Chiung-Wen Kuo ◽  
Di-Yen Chueh ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Conducting Polymer ◽  
Circulating Tumor Cell ◽  
Cell Capture ◽  
Capture And Release

3D conducting polymer-based bioelectronic interface (BEI) devices for dynamically controlling circulating tumor cell capture/release performance through the cyclic potential of electrical stimulation.

Circulating tumor cell detection using a parallel flow micro-aperture chip system

Lab on a Chip ◽  
2015 ◽  
Vol 15 (7) ◽  
pp. 1677-1688 ◽  
Author(s):  
Chun-Li Chang ◽  
Wanfeng Huang ◽  
Shadia I. Jalal ◽  
Bin-Da Chan ◽  
Aamer Mahmood ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Circulating Tumor Cell ◽  
Parallel Flow ◽  
Cell Detection ◽  
Tumor Cell Detection ◽  
System P

A parallel flow micro-aperture chip system for detection of circulating tumor cells.

scholarly journals Microfluidics-enabled rapid manufacturing of hierarchical silica-magnetic microflower toward enhanced circulating tumor cell screening

2018 ◽  
Vol 6 (12) ◽  
pp. 3121-3125 ◽  
Author(s):  
Nanjing Hao ◽  
Yuan Nie ◽  
Amogha Tadimety ◽  
Ting Shen ◽  
John X. J. Zhang
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Circulating Tumor Cell ◽  
Rapid Manufacturing ◽  
Screening Efficiency

Microfluidics-enabled rapid manufacturing of a hierarchical silica-magnetic microflower was developed for improving the screening efficiency of circulating tumor cells.

scholarly journals Nanotechnology-Assisted Isolation and Analysis of Circulating Tumor Cells on Microfluidic Devices

Micromachines ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 774 ◽  
Author(s):  
Jie Cheng ◽  
Yang Liu ◽  
Yang Zhao ◽  
Lina Zhang ◽  
Lingqian Zhang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Liquid Biopsy ◽  
Cancer Metastasis ◽  
Human Peripheral Blood ◽  
Microfluidic Devices ◽  
Detection Sensitivity ◽  
Cell Capture ◽  
Primary Tumors ◽  
Downstream Analysis

Circulating tumor cells (CTCs), a type of cancer cell that spreads from primary tumors into human peripheral blood and are considered as a new biomarker of cancer liquid biopsy. It provides the direction for understanding the biology of cancer metastasis and progression. Isolation and analysis of CTCs offer the possibility for early cancer detection and dynamic prognosis monitoring. The extremely low quantity and high heterogeneity of CTCs are the major challenges for the application of CTCs in liquid biopsy. There have been significant research endeavors to develop efficient and reliable approaches to CTC isolation and analysis in the past few decades. With the advancement of microfabrication and nanomaterials, a variety of approaches have now emerged for CTC isolation and analysis on microfluidic platforms combined with nanotechnology. These new approaches show advantages in terms of cell capture efficiency, purity, detection sensitivity and specificity. This review focuses on recent progress in the field of nanotechnology-assisted microfluidics for CTC isolation and detection. Firstly, CTC isolation approaches using nanomaterial-based microfluidic devices are summarized and discussed. The different strategies for CTC release from the devices are specifically outlined. In addition, existing nanotechnology-assisted methods for CTC downstream analysis are summarized. Some perspectives are discussed on the challenges of current methods for CTC studies and promising research directions.

scholarly journals Screening Circulating Tumor Cells as a Noninvasive Cancer Test in 3388 Individuals from High-Risk Groups (ICELLATE2)

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Juan Castro ◽  
Luis Sanchez ◽  
María Teresa Nuñez ◽  
Ming Lu ◽  
Tomas Castro ◽  
...  
Keyword(s):  
High Risk ◽  
Early Detection ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Clinical Symptoms ◽  
Risk Groups ◽  
Circulating Tumor Cell ◽  
High Risk Group ◽  
Screening Tests ◽  
Cancer Risk Factors

Cancer is known to spread up to 12 years before clinical symptoms occur, but few screening tests exist. Early detection would give the opportunity for early treatment, potentially improving prognosis. To this end, 3388 subjectively healthy individuals of age 45 to 80 who had been exposed to cancer risk factors were screened for the occurrence of circulating tumor cells in their blood. Presence of circulating tumor cells is a suspicious finding indicative of spreading cancer, since cancer metastasizes by way of the blood and offers the opportunities to (a) follow up the individual clinically based on established guidelines for early detection of cancer and (b) evaluate the cells further analytically. 107 individuals showed one or more circulating tumor cells in a 7.5 ml blood sample, which constitutes a positive circulating tumor cell test, based on the iCellate IsoPic™ laboratory test. That number compares favorably with the cancer incidence per 100,000 people/year that is 157.1 in Peru, given that a high-risk group of individuals was screened and that the screening results would be expected to correspond to an accumulated incidence of up to 12 years. The present findings therefore identify screening for circulating tumor cells as a promising new test.

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