scholarly journals Phosphoprotein analysis of soybean root hairs in response to Bradyrhizobium japonicum inoculation

2012 ◽  
Author(s):  
Tran Hong Nha Nguyen
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Root Hairs ◽  
Soybean Root

scholarly journals Proteomic Analysis of Soybean Root Hairs After Infection by Bradyrhizobium japonicum

2005 ◽  
Vol 18 (5) ◽  
pp. 458-467 ◽  
Author(s):  
Jinrong Wan ◽  
Michael Torres ◽  
Ashwin Ganapathy ◽  
Jay Thelen ◽  
Beverly B. DaGue ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Polyacrylamide Gel Electrophoresis ◽  
Root Hairs ◽  
Ionization Time ◽  
Soybean Root ◽  
Novel Proteins ◽  
Rhizobial Inoculation ◽  
Proteomic Study ◽  
Complex Events ◽  
Root Hair Infection

Infection of soybean root hairs by Bradyrhizobium japonicum is the first of several complex events leading to nodulation. In the current proteomic study, soybean root hairs after inoculation with B. japonicum were separated from roots. Total proteins were analyzed by two-dimensional (2-D) polyacrylamide gel electrophoresis. In one experiment, 96 protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) to compare protein profiles between uninoculated roots and root hairs. Another 37 spots, derived from inoculated root hairs over different timepoints, were also analyzed by tandem MS (MS/MS). As expected, some proteins were differentially expressed in root hairs compared with roots (e.g., a chitinase and phosphoenolpyruvate carboxylase). Out of 37 spots analyzed by MS/MS, 27 candidate proteins were identified by database comparisons. These included several proteins known to respond to rhizobial inoculation (e.g., peroxidase and phenylalanine-ammonia lyase). However, novel proteins were also identified (e.g., phospholipase D and phosphoglucomutase). This research establishes an excellent system for the study of root-hair infection by rhizobia and, in a more general sense, the functional genomics of a single, plant cell type. The results obtained also indicate that proteomic studies with soybean, lacking a complete genome sequence, are practical.

scholarly journals Quantitative Phosphoproteomic Analysis of Soybean Root Hairs Inoculated with Bradyrhizobium japonicum

2012 ◽  
Vol 11 (11) ◽  
pp. 1140-1155 ◽  
Author(s):  
Tran Hong Nha Nguyen ◽  
Laurent Brechenmacher ◽  
Joshua T. Aldrich ◽  
Therese R. Clauss ◽  
Marina A. Gritsenko ◽  
...  
Keyword(s):  
Root Hair ◽  
Bradyrhizobium Japonicum ◽  
Cell Biology ◽  
Magnetic Beads ◽  
Critical Role ◽  
Root Hairs ◽  
Root Surface ◽  
Infection Process ◽  
Root Surface Area ◽  
Soybean Root

Root hairs are single hair-forming cells on roots that function to increase root surface area, enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role as the site of infection by symbiotic nitrogen fixing rhizobia, leading to the formation of a novel organ, the nodule. The initial steps in the rhizobia-root hair infection process are known to involve specific receptor kinases and subsequent kinase cascades. Here, we characterize the phosphoproteome of the root hairs and the corresponding stripped roots (i.e. roots from which root hairs were removed) during rhizobial colonization and infection to gain insight into the molecular mechanism of root hair cell biology. We chose soybean (Glycine max L.), one of the most important crop plants in the legume family, for this study because of its larger root size, which permits isolation of sufficient root hair material for phosphoproteomic analysis. Phosphopeptides derived from root hairs and stripped roots, mock inoculated or inoculated with the soybean-specific rhizobium Bradyrhizobium japonicum, were labeled with the isobaric tag eight-plex iTRAQ, enriched using Ni-NTA magnetic beads and subjected to nanoRPLC-MS/MS1 analysis using HCD and decision tree guided CID/ETD strategy. A total of 1625 unique phosphopeptides, spanning 1659 nonredundant phosphorylation sites, were detected from 1126 soybean phosphoproteins. Among them, 273 phosphopeptides corresponding to 240 phosphoproteins were found to be significantly regulated (>1.5-fold abundance change) in response to inoculation with B. japonicum. The data reveal unique features of the soybean root hair phosphoproteome, including root hair and stripped root-specific phosphorylation suggesting a complex network of kinase-substrate and phosphatase-substrate interactions in response to rhizobial inoculation.

scholarly journals Soybean root system under the effect of Bradyrhizobium japonicum

2018 ◽  
Vol 0 (1) ◽  
pp. 138-143
Author(s):  
Ірина Ігорівна Гуменюк ◽  
Сергій Юрійович Грузінський ◽  
Ірина Степанівна Бровко ◽  
Ярослав Васильович Чабанюк
Keyword(s):  
Root System ◽  
Bradyrhizobium Japonicum ◽  
Soybean Root

scholarly journals Negative short-term salt effects on the soybean–Bradyrhizobium japonicum interaction and partial reversion by calcium addition

2014 ◽  
Vol 41 (1) ◽  
pp. 96 ◽  
Author(s):  
Nacira Muñoz ◽  
Marianela Rodriguez ◽  
German Robert ◽  
Ramiro Lascano
Keyword(s):  
Salt Stress ◽  
Root Hair ◽  
Bradyrhizobium Japonicum ◽  
Plant Pathogens ◽  
Root Hairs ◽  
Short Term ◽  
Calcium Addition ◽  
Negative Effect ◽  
Osmotic Treatment ◽  
Related Proteins

The short-term (2 h) effects of salt stress (50 and 150 mM NaCl) on early events of soybean– Bradyrhizobium japonicum (rhizobia) interaction were analysed, determining the following parameters in root hair with or without calcium addition: deformation, apoplastic superoxide radical production (O2⚫–), root hair death and sodium/potassium ion content. We also analysed whether this short-term salt stress influenced later formation of crown and noncrown nodules, determining the number and weight of nodules. The negative effect of salt stress on these characters was attenuated by the addition of 5 mM CaCl2. We also analysed the expression of pathogenesis-related proteins (PRP) genes PR-1, PR-2, PR-3, and four isoforms of PR-5. The expression of PR-2 increased under saline conditions and decreased in osmotic treatment and saline treatment supplemented with calcium in the presence of the symbiont. The changes in PR-2 expression levels, together with the death of root hairs provide a possible mechanism for the inhibition of infection by the symbiont under salinity, and suggests a possible overlap with responses to plant pathogens.

scholarly journals Complete Transcriptome of the Soybean Root Hair Cell, a Single-Cell Model, and Its Alteration in Response to Bradyrhizobium japonicum Infection

2009 ◽  
Vol 152 (2) ◽  
pp. 541-552 ◽  
Author(s):  
Marc Libault ◽  
Andrew Farmer ◽  
Laurent Brechenmacher ◽  
Jenny Drnevich ◽  
Raymond J. Langley ◽  
...  
Keyword(s):  
Hair Cell ◽  
Single Cell ◽  
Root Hair ◽  
Bradyrhizobium Japonicum ◽  
Cell Model ◽  
Soybean Root ◽  
Root Hair Cell ◽  
Single Cell Model

Curled root hairs are a site of entry for Bradyrhizobium infecting hydroponically grown soybean plants with mature root systems

1988 ◽  
Vol 66 (4) ◽  
pp. 683-686 ◽  
Author(s):  
Jeanne M. L. Selker ◽  
John Imsande ◽  
Eldon H. Newcomb
Keyword(s):  
Glycine Max ◽  
Root Hair ◽  
Bradyrhizobium Japonicum ◽  
Root Hairs ◽  
Root Systems ◽  
Glycine Max L ◽  
Soybean Plants ◽  
A Site ◽  
Hydroponically Grown ◽  
Mature Root

Early emergent nodules on roots of hydroponically grown soybean plants (Glycine max (L.) Merr.) were sectioned serially to locate the site of infection by Bradyrhizobium japonicum. The plants had been inoculated only after their root systems had produced numerous higher order branches. The hydroponic solutions contained all required nutrients, including either a suboptimal concentration of nitrate (0.5 mM) or an excess of nitrate (4.0 mM). In all six nodules examined, three with suboptimal nitrate and three with excess nitrate, we found a centrally located root hair containing an infection thread. We conclude that mature root systems of soybean grown in aqueous culture can undergo infection through root hairs in the way that is typical of young seedlings grown either in pots of vermiculite or pouches.

Particle density and protein composition of the peribacteroid membrane from soybean root nodules is affected by mutation in the microsymbiont Bradyrhizobium japonicum

Planta ◽  
1988 ◽  
Vol 174 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Dietrich Werner ◽  
Erhard M�rschel ◽  
Christine Garbers ◽  
Stefan Bassarab ◽  
Robert B. Mellor
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Particle Density ◽  
Root Nodules ◽  
Protein Composition ◽  
Peribacteroid Membrane ◽  
Soybean Root

scholarly journals Increase in root nodulation and crop yield of soybean by native Bradyrhizobium japonicum strains

2010 ◽  
Vol 6 ◽  
pp. 1-3
Author(s):  
Namraj Dhami ◽  
Braj Nandan Prasad
Keyword(s):  
Glycine Max ◽  
Crop Yield ◽  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Bromothymol Blue ◽  
Strong Positive Correlation ◽  
N Content ◽  
Root Nodulation ◽  
Soybean Root ◽  
Gram Staining

Native strains of Bradyrhizobium japonicum were tested for their effectiveness on nodulation, crop yield and nitrogen fixation in soybean (Glycine max). B. japonicum strains were isolated from soybean root nodules collected from different agro-climatic regions of Far Western Nepal, viz. Dipayal (607 m asl), Dadeldhura (1097 m asl), Silgadhi (1209 m asl) and Bajura (1524 m asl). The strains were characterized by studying colony characteristics, growth response with Congo red and Bromothymol blue, and Gram staining. The native bradyrhizobial strains were authenticated by performing infection test on soybean seedlings. All the four strains were found compatible and effective on root nodulation, crop yield and soil nitrogen (N) content. Inoculation of these strains increased soybean root nodulation by 247-343% and crop yield by 45-204%. There was strong positive correlation (r = 0.982) between number of root nodules and crop yield, which suggest that optimization of root nodulation by inoculating compatible and effective B. japonicum strains significantly increase the soybean crop yield. Soil N content of inoculated experimental pots was increased by 13-33%. However, variability among different strains was observed in their effect on root nodulation and yield performance. B. japonicum strain collected from Silgadhi was found to be the most effective in increasing nodule number and crop yield by 343% and 204% respectively. Key-words: Glycine max; gram staining; inoculation; nitrogen fixationDOI: 10.3126/botor.v6i0.2902 Botanica Orientalis - Journal of Plant Science (2009) 6: 1-3

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