Particle density and protein composition of the peribacteroid membrane from soybean root nodules is affected by mutation in the microsymbiont Bradyrhizobium japonicum

Planta ◽  
1988 ◽  
Vol 174 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Dietrich Werner ◽  
Erhard M�rschel ◽  
Christine Garbers ◽  
Stefan Bassarab ◽  
Robert B. Mellor
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Particle Density ◽  
Root Nodules ◽  
Protein Composition ◽  
Peribacteroid Membrane ◽  
Soybean Root

scholarly journals Increase in root nodulation and crop yield of soybean by native Bradyrhizobium japonicum strains

2010 ◽  
Vol 6 ◽  
pp. 1-3
Author(s):  
Namraj Dhami ◽  
Braj Nandan Prasad
Keyword(s):  
Glycine Max ◽  
Crop Yield ◽  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Bromothymol Blue ◽  
Strong Positive Correlation ◽  
N Content ◽  
Root Nodulation ◽  
Soybean Root ◽  
Gram Staining

Native strains of Bradyrhizobium japonicum were tested for their effectiveness on nodulation, crop yield and nitrogen fixation in soybean (Glycine max). B. japonicum strains were isolated from soybean root nodules collected from different agro-climatic regions of Far Western Nepal, viz. Dipayal (607 m asl), Dadeldhura (1097 m asl), Silgadhi (1209 m asl) and Bajura (1524 m asl). The strains were characterized by studying colony characteristics, growth response with Congo red and Bromothymol blue, and Gram staining. The native bradyrhizobial strains were authenticated by performing infection test on soybean seedlings. All the four strains were found compatible and effective on root nodulation, crop yield and soil nitrogen (N) content. Inoculation of these strains increased soybean root nodulation by 247-343% and crop yield by 45-204%. There was strong positive correlation (r = 0.982) between number of root nodules and crop yield, which suggest that optimization of root nodulation by inoculating compatible and effective B. japonicum strains significantly increase the soybean crop yield. Soil N content of inoculated experimental pots was increased by 13-33%. However, variability among different strains was observed in their effect on root nodulation and yield performance. B. japonicum strain collected from Silgadhi was found to be the most effective in increasing nodule number and crop yield by 343% and 204% respectively. Key-words: Glycine max; gram staining; inoculation; nitrogen fixationDOI: 10.3126/botor.v6i0.2902 Botanica Orientalis - Journal of Plant Science (2009) 6: 1-3

An integrated proteomics and transcriptomics reference data set provides new insights into the Bradyrhizobium japonicum bacteroid metabolism in soybean root nodules

PROTEOMICS ◽  
2010 ◽  
Vol 10 (7) ◽  
pp. 1391-1400 ◽  
Author(s):  
Nathanaël Delmotte ◽  
Christian H. Ahrens ◽  
Claudia Knief ◽  
Ermir Qeli ◽  
Marion Koch ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Reference Data ◽  
Root Nodules ◽  
Data Set ◽  
Soybean Root

Peribacteroid solution of soybean root nodules partly induces genomic loci for differentiation into bacteroids of free-living Bradyrhizobium japonicum cells

2015 ◽  
Vol 61 (3) ◽  
pp. 461-470 ◽  
Author(s):  
Naoko Ohkama-Ohtsu ◽  
Sachiko Ichida ◽  
Hiroko Yamaya ◽  
Takuji Ohwada ◽  
Manabu Itakura ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Free Living ◽  
Soybean Root

scholarly journals Genome-Wide Transcript Analysis of Bradyrhizobium japonicum Bacteroids in Soybean Root Nodules

2007 ◽  
Vol 20 (11) ◽  
pp. 1353-1363 ◽  
Author(s):  
Gabriella Pessi ◽  
Christian H. Ahrens ◽  
Hubert Rehrauer ◽  
Andrea Lindemann ◽  
Felix Hauser ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Experimental Approach ◽  
Root Nodules ◽  
Nodule Development ◽  
Dependent Manner ◽  
Wild Type ◽  
In Planta ◽  
Soybean Root ◽  
Genome Wide ◽  
Transcript Profiles

The transcriptome of endosymbiotic Bradyrhizobium japonicum bacteroids was assessed, using RNA extracted from determinate soybean root nodules. Results were compared with the transcript profiles of B. japonicum cells grown in either aerobic or microaerobic culture. Microoxia is a known trigger for the induction of symbiotically relevant genes. In fact, one third of the genes induced in bacteroids at day 21 after inoculation are congruent with those up-regulated in culture by a decreased oxygen concentration. The other induced genes, however, may be regulated by cues other than oxygen limitation. Both groups of genes provide a rich source for the possible discovery of novel functions related to symbiosis. Samples taken at different timepoints in nodule development have led to the distinction of genes expressed early and late in bacteroids. The experimental approach applied here is also useful for B. japonicum mutant analyses. As an example, we compared the transcriptome of wild-type bacteroids with that of bacteroids formed by a mutant defective in the RNA polymerase transcription factor σ54. This led to a collection of hitherto unrecognized B. japonicum genes potentially transcribed in planta in a σ54-dependent manner.

Expression of nir, nor and nos denitrification genes from Bradyrhizobium japonicum in soybean root nodules

2004 ◽  
Vol 120 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Socorno Mesa ◽  
Juan de Dios Alche ◽  
Eulogio Bedmar ◽  
Maria J. Delgado
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Soybean Root ◽  
Denitrification Genes

The Occurrence of Choline Kinase II in the Cytoplasm of Soybean Root Nodules Infected with Various Strains of Bradyrhizobium japonicum

1987 ◽  
Vol 128 (1-2) ◽  
pp. 169-172 ◽  
Author(s):  
Robert B. Mellor ◽  
Heidemarie Thierfelder ◽  
Gudrun Pausch ◽  
Dietrich Werner
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Root Nodules ◽  
Choline Kinase ◽  
Soybean Root
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