scholarly journals Intestinal Alkaline Phosphatase to Total Alkaline Phosphatase Ratio Measurement

2020 ◽  
Author(s):  
Keyword(s):  
Alkaline Phosphatase ◽  
Intestinal Alkaline Phosphatase ◽  
Total Alkaline Phosphatase ◽  
Ratio Measurement ◽  
Total Alkaline

scholarly journals Multiple forms of alkaline phosphatase in plasma of hemodialysis patients

1991 ◽  
Vol 37 (6) ◽  
pp. 815-820 ◽  
Author(s):  
L Tibi ◽  
S C Chhabra ◽  
V M Sweeting ◽  
R J Winney ◽  
A F Smith
Keyword(s):  
Alkaline Phosphatase ◽  
Total Activity ◽  
Bone Alkaline Phosphatase ◽  
Intestinal Alkaline Phosphatase ◽  
Total Alkaline Phosphatase ◽  
Gamma Glutamyltransferase ◽  
Biochemical Indices ◽  
Increased Activity ◽  
Separate Measurement ◽  
Total Alkaline

Abstract We used quantitative assays to measure the activity of the bone, liver, and intestinal forms of alkaline phosphatase in plasma in 75 patients with endstage chronic renal failure undergoing hemodialysis. The results were correlated with radiological and other biochemical indices of bone disease and with biochemical indices of liver disease. The total activity of alkaline phosphatase in plasma increased in 28 patients. In 10 of these patients, nine of whom had increased activity of gamma-glutamyltransferase in plasma, the increase in total activity of alkaline phosphatase was from the liver isoenzyme alone (nine patients) or from the liver and bone isoenzymes together (one patient). Intestinal alkaline phosphatase in plasma, although greater than 23 U/L in eight patients, was solely responsible for the increase in total alkaline phosphatase in one patient (who had normal gamma-glutamyltransferase). Bone alkaline phosphatase in plasma was increased in 25 patients, seven of whom had normal total alkaline phosphatase, and was closely correlated (r = 0.78) with osteocalcin concentration in plasma, which was increased in a much greater proportion of patients (99%). Both total and bone alkaline phosphatase were correlated with parathyrin in plasma (r = 0.46 and 0.50, respectively) and with osteocalcin (r = 0.60 and 0.78, respectively). Osteocalcin and bone alkaline phosphatase, but not parathyrin, decreased with age, implying that the skeletal response to parathyrin may be age dependent. In patients with increased total alkaline phosphatase undergoing hemodialysis, the concurrent measurement of gamma-glutamyltransferase may help identify whether the enzyme increase originates from the liver or bone, but this approach wrongly identified the source of the increase in three of 28 patients. Therefore, we recommend a separate measurement of the bone isoenzyme of alkaline phosphatase.

scholarly journals Bone mineral density and serum levels of 25 OH vitamin D in chronic users of antiepileptic drugs

2004 ◽  
Vol 62 (4) ◽  
pp. 940-948 ◽  
Author(s):  
Carolina A.M. Kulak ◽  
Victória Z.C. Borba ◽  
John P. Bilezikian ◽  
Carlos E. Silvado ◽  
Luciano de Paola ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Vitamin D ◽  
Alkaline Phosphatase ◽  
Lumbar Spine ◽  
Antiepileptic Drugs ◽  
Serum Levels ◽  
Mineral Density ◽  
Total Alkaline Phosphatase ◽  
Blood Samples ◽  
Total Alkaline

The aim of this cross sectional study was to evaluate bone mineral density (BMD) and serum levels of 25-hydroxy vitamin D (25OHD) in a group of patients taking antiepileptic drugs (AED) for a seizure disorder. Between May-2001 and January-2003, we evaluated 58 patients (40 women/18 men), 34.4±6 years old living in Curitiba or in its metropolitan area, on antiepileptic therapy for 2 to 38 years (10 on monotherapy /48 on multiple drugs regime). The group was matched by age, gender, and bone mass index to 29 healthy subjects (20 women/ 9 men); 34.2±5.9 years old. Medical history and physical exam were performed on all subjects with particular information sought about fractures and risks factors for osteoporosis. Blood samples were collected for total serum calcium, albumin, phosphorus, creatinine, total alkaline phosphatase, and liver function tests. BMD of the lumbar spine, femur and forearm was determined by dual energy X-ray absorptiometry (DXA, Hologic QDR 1000). Between February and April-2003, other blood samples were collected to measure 25OHD, intact paratohormone (PTH) and calcium. Unemployment and smoking history were more frequent among patients than among controls (p<0.05). Fifteen patients had a fracture history, all of which occurred during a seizure. The BMD of the lumbar spine (0.975±0. 13 g/cm² vs. 1.058±0.1 g/cm²; p<0.03) and of the total femur (0.930±0.1 g/cm² vs. 0.988±0.12 g/cm²; p<0.02) was lower in patients than in controls. In 63.5% of patients and in 24.1 % of controls a T-score < -1.0 in at least one site was seen. The AED users had higher total alkaline phosphatase and lower 25OHD (p<0.02). No correlations between BMD and 25OHD were found. The use of phenytoin was correlated with a greater incidence of fractures (RR: 2.38). We conclude that patients on chronic use of AED have alterations in bone metabolism characterized in this study by lower BMD of the lumbar spine and total femur and lower serum concentrations of 25OHD.

A New Fluorometric Method for the Estimation or Detection of Total and Fractionated Alkaline Phosphatase

1969 ◽  
Vol 15 (2) ◽  
pp. 108-123 ◽  
Author(s):  
Roy B Johnson
Keyword(s):  
Alkaline Phosphatase ◽  
Relative Activity ◽  
Normal Serum ◽  
Starch Gel Electrophoresis ◽  
Total Alkaline Phosphatase ◽  
Fluorometric Method ◽  
Highly Sensitive ◽  
Starch Gel ◽  
Alpha Beta ◽  
Total Alkaline

Abstract A highly sensitive fluorometric method for the assay of total alkaline phosphatase and the detection of its components is described. The commercially available substrate naphthyl AS-MX phosphate, combined in 1 M 2-amino-2-methyl-1-propanol buffer, pH 9.8, is cleaved to the highly fluorescent naphthol AS-MX. Fluorometry requires filters passing 405 mµ primary and 505 mµ secondary. As little as 20 µl. of normal serum per 3 ml. of reaction mixture can be assayed. Alkaline phosphatase components, separated by vertical starch gel (or other methods of) electrophoresis, produce yellow fluorescence under ultraviolet light when incubated at 37° with the same buffer-substrate. After vertical starch gel electrophoresis, all normal serums exhibit at least one component (β-globulin region), but six distinct areas of activity have been located. These correspond to Taswell and Jeffers’ origin, beta-lipoprotein, alpha-2, alpha-beta, and beta (5). Few serums contain all of these; rather there appears to be a correlation between the ones present, their relative activity, and the disease state.

scholarly journals Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C.

1977 ◽  
Vol 167 (1) ◽  
pp. 281-284 ◽  
Author(s):  
M G Low ◽  
J B Finean
Keyword(s):  
Staphylococcus Aureus ◽  
Alkaline Phosphatase ◽  
Phospholipase C ◽  
Mammalian Species ◽  
Salt Solutions ◽  
Total Alkaline Phosphatase ◽  
Total Alkaline Phosphatase Activity ◽  
Wide Range ◽  
Releasing Effect ◽  
Total Alkaline

Purified phosphatidylinositol-specific phospholipase C from Staphylococcus aureus released a substantial proportion of the total alkaline phosphatase activity from a wide range of tissues from several mammalian species. Co-purification of the phospholipase C and alkaline phosphatase-releasing activities and the inhibition of both these activities by iso-osmotic salt solutions suggested that the releasing effect was unlikely to be due to a contaminant.

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