scholarly journals Hemoglobin A2 Measurement

2020 ◽  
Author(s):  
Keyword(s):  
Hemoglobin A2

"FAST" HEMOGLOBIN COMPONENT FOUND IN UMBILICAL-CORD BLOOD OF THAI BABIES

PEDIATRICS ◽  
1959 ◽  
Vol 24 (1) ◽  
pp. 43-49
Author(s):  
Soodsarkorn Tuchinda ◽  
Chitra Vareenil ◽  
Partraporn Bhanchit ◽  
Virginia Minnich
Keyword(s):  
Cord Blood ◽  
Saline Solution ◽  
Fetal Hemoglobin ◽  
Paper Electrophoresis ◽  
Fast Component ◽  
Thai Population ◽  
Hemoglobin E ◽  
Normal Limits ◽  
Abnormal Hemoglobin ◽  
Hemoglobin A2

Four hundred and fifteen specimens of cord blood collected from the Thai population were examined; 22 contained an electrophoretically "fast"-moving component of hemoglobin, an incidence of 5.2%. No other abnormal hemoglobin was found in the specimens of cord blood. Eleven of the babies with abnormal hemoglobin were examined; hematologic findings were within normal limits. Five were followed for 2½ to 8 months; the abnormal component disappeared within 76 to 101 days. One of these children subsequently developed hemoglobin E. Another baby, 2 days of age when first seen, had the "fast" component of hemoglobin in the blood. She was the only child in the group who had marked hepatomegaly, splenomegaly, reticulocytosis, and nucleated erythrocytes in the peripheral blood. These abnormalities subsequently disappeared, but at 112 days a small amount of the "fast" component of hemoglobin persisted and hemoglobin E had appeared. The blood of 412 mothers revealed an incident of 10% hemoglobin E, the only abnormal hemoglobin detected by paper electrophoresis. The concentration of hemoglobin A2 was determined on the blood drawn from nine parents of babies with abnormal "fast" hemoglobin; the value was normal in all instances. Microcytosis and increased resistance of the erythrocytes in hypotonic saline solution, however, was found frequently among the parents of affected babies. The abnormal "fast" component of hemoglobin was identical with "Barts" hemoglobin. Its characteristics suggest that it may be an abnormal fetal hemoglobin.

Hemoglobin A2-indonesia or α2δ269(E13)Gly→Arg

1971 ◽  
Vol 229 (2) ◽  
pp. 335-342 ◽  
Author(s):  
Lie Injo Luan Eng ◽  
Wita Pribadi ◽  
F. Westendorp Boerma ◽  
G.D. Efremov ◽  
J.B. Wilson ◽  
...  
Keyword(s):  
Hemoglobin A2

Screening for hemoglobinopathy with capillary electrophoresis in adult patients

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S18-S18
Author(s):  
Bremansu Osa-Andrews ◽  
Nicole Desimone ◽  
Ravi Sarode ◽  
Sarita Paulino ◽  
Jing Cao
Keyword(s):  
Bone Marrow ◽  
Capillary Electrophoresis ◽  
Gel Electrophoresis ◽  
Screening Method ◽  
Adult Patients ◽  
Red Blood Cell Indices ◽  
Hemoglobin Variants ◽  
Clinically Significant ◽  
Hemoglobin A2 ◽  
Rule Out

Abstract Hemoglobinopathy screening is frequently needed in adult patients, including prenatal carrier screen, workup of unexplained anemia, and bone marrow donor and recipient screening. However, the preferred test method for screening of hemoglobinopathy is not well established due to limited guidance from professional societies. American College of Obstetricians and Gynecologists’ Committee on Genetics recommended hemoglobin electrophoresis as the screening method of hemoglobinopathy in pregnancy; nevertheless, electrophoresis employs various methodologies, including acid gel electrophoresis, alkaline gel electrophoresis, and capillary electrophoresis (CE) with alkaline buffer. For other adult patient populations who need hemoglobinopathy screening, no clear guidelines dictate the method of choice. A previous study has shown that CE captures major hemoglobinopathies with comparable performance to high-performance liquid chromatography (HPLC) in pediatric patients but no study has investigated using CE alone in adult patient screening. In this retrospective study, we evaluated the utility of CE as a screening method to rule out clinically significant hemoglobin variants. During eight months, 312 adult patients without previously identified hemoglobin variants had hemoglobinopathy screening performed using a comprehensive testing algorithm. This cascade algorithm screens for hemoglobinopathy using both CE (Capillarys, Sebia, Paris, France) and HPLC (laboratory-developed test) with reflex to more advanced variant identification such as mass spectrometry and genetic analyses. Categories of abnormal findings were reviewed to determine if hemoglobinopathy can be identified by using CE only. The patient population mainly consists of pregnant women and anemic patients with hematologic malignancies with an average age of 42. Out of the 312 screened patients, 47 had abnormal results. The most frequent condition was elevated hemoglobin F (N=25) ranging 2-5% seen in leukemia patients on chemotherapy attributed to bone marrow stress. Eight cases of beta plus thalassemia (featuring hemoglobin A2 >4%) and 3 cases of hemoglobin C trait were identified in patients with little to mild clinical manifestations (red blood cell indices suggesting anemia). Decreased hemoglobin A2 fraction was observed in 7 patients, and potential causes were alpha thalassemia or iron deficiency. Other less common hemoglobinopathies included heterozygote A2 prime (N=3, a benign delta chain variant that migrates separately from hemoglobin A2 on CE) and hemoglobin G-Philadelphia (N=1). All of the abnormal results are identifiable by CE alone, although HPLC and more advanced methods help confirm the diagnosis. Our study shows that CE as the first line of screening method would rule out major hemoglobinopathies in adults. There have been reports that rare but clinically significant hemoglobin variants like hemoglobin Malmo may not be detected by CE, and therefore, certain pre-test probability factors need to be considered when testing for hemoglobinopathies, such as race/ethnicity background, family history, red blood cell indices, and iron deficiency status.

Improved Method for the Determination of Hemoglobin A2 by Starch-Gel Electrophoresis

1960 ◽  
Vol 6 (3) ◽  
pp. 254-262 ◽  
Author(s):  
C A J. Goldberg ◽  
A C Ross
Keyword(s):  
Sickle Cell ◽  
Gel Electrophoresis ◽  
Sickle Cell Trait ◽  
Starch Gel Electrophoresis ◽  
Improved Method ◽  
Starch Gel ◽  
Thalassemia Trait ◽  
Healthy Persons ◽  
Hemoglobin A2

Abstract It has been shown that variations in the preparation of the starch gel and in photographic interpretation can significantly affect the accuracy of the measurement of hemoglobin A2. An improved method for the determination of hemoglobin A2 by starch-gel electrophoresis has been presented which affords greater accuracy than was previously achieved. Hemoglobin A2 concentrations for healthy persons and patients with sickle cell trait, various nonthalassemic anemias, and thalassemia trait have been presented.

Rapid estimation of hemoglobin A2 by DEAE chromatography

1969 ◽  
Vol 2 (4) ◽  
pp. 305-310 ◽  
Author(s):  
L. F. Bernini
Keyword(s):  
Rapid Estimation ◽  
Hemoglobin A2

Improved batch and column separation in the assay of hemoglobin A2.

1976 ◽  
Vol 22 (12) ◽  
pp. 2036-2038 ◽  
Author(s):  
L G Morin
Keyword(s):  
Buffer System ◽  
Hemoglobin S ◽  
Column Separation ◽  
Column Method ◽  
Diethylaminoethyl Cellulose ◽  
Hemoglobin A2

Abstract Hemoglobin A2 is a batch fractionated on a column containing diethylaminoethyl-cellulose (DE52, Whatman) with a discontinuous buffer system at pH 8.9 and 7.3. A short micro-column method is also presented, which allows separation in less than 30 min with no interference from hemoglobin S. Both methods clearly distinguish normal from thalassemia specimens, and are simpler and more rapid than previously published methods.

scholarly journals Glycosylated hemoglobin A2 components

Blood ◽  
1980 ◽  
Vol 56 (3) ◽  
pp. 571-572 ◽  
Author(s):  
C Tegos ◽  
E Beutler
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Glycosylated Hemoglobin ◽  
Diabetic Patients ◽  
Agar Gel ◽  
Hemoglobin A ◽  
Hemoglobin A1 ◽  
Agar Gel Electrophoresis ◽  
Derivatives Of ◽  
Hemoglobin A2

Abstract Partially purified hemoglobin A2 has been examined for the existence of glycosylated components by isoelectric focusing and by acid agar gel electrophoresis. Bands analogous to the glycohemoglobin derivatives of hemoglobin A, hemoglobin-A1.a.b.c, were readily detected. Evidence that these minor bands are in fact glycohemoglobins was obtained by showing that 14C-glucose bound to hemoglobin A2 moved with these minor bands. The amounts of glycohemoglobin derivatives of hemoglobin A2 were increased in the blood of diabetic patients.

A New Method for Starch Gel Electrophoresis of Human Hemoglobins, with Special Reference to the Determination of Hemoglobin A2

1958 ◽  
Vol 4 (6) ◽  
pp. 484-495 ◽  
Author(s):  
C A J Goldberg
Keyword(s):  
Special Reference ◽  
Gel Electrophoresis ◽  
New Method ◽  
Resolving Power ◽  
Buffer System ◽  
Starch Gel Electrophoresis ◽  
Healthy Individuals ◽  
Starch Gel ◽  
Hemoglobin A2

Abstract A method for starch gel electrophoresis of hemoglobins is presented in which a modified Lintner starch is used for the preparation of the gel. A discontinuous buffer system of tris-EDTA-borate/barbital is used as the electrolyte medium because of its superior resolving power. Hemoglobin A2 values, obtained with this method, of healthy individuals, patients with thalassemia, and those with various anemias of nonthalassemic origin are presented.

scholarly journals Higher Sensitivity of Capillary Electrophoresis in Detecting Hemoglobin A2'

2012 ◽  
Vol 138 (suppl 2) ◽  
pp. A186-A186
Author(s):  
Deanna Oleske ◽  
Richard S.P. Huang ◽  
Amitava Dasgupta ◽  
Andy Nguyen ◽  
Amer Wahed
Keyword(s):  
Capillary Electrophoresis ◽  
Hemoglobin A2 ◽  
Higher Sensitivity
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