Rapid estimation of hemoglobin A2 by DEAE chromatography

1969 ◽  
Vol 2 (4) ◽  
pp. 305-310 ◽  
Author(s):  
L. F. Bernini
1975 ◽  
Vol 21 (10) ◽  
pp. 1490-1492 ◽  
Author(s):  
Leo G Morin

Abstract Hemoglobin A2 is batch fractionated with diethylaminoethyl Bio-Gel A (Bio-Rad Laboratories) equilibrated with a tris(hydroxymethyl)aminomethane HCl buffer (pH 7.68, 8.75 mmol/liter, 6.36 mmol of Cl- per liter). Hemoglobin A1 and F become bound to the resin, allowing the separation and quantitation of A2. Hemoglobin S alters the equilibrium condition, but adjustments are easily made so that A2 can be separated in the presence of S. The procedure is simpler than electrophoretic or chromatographic methods, requires 5 min, is accurate (A2 fraction is at least 94% A2, less than 6% A1), precise (SD ±0.24 for duplicates), and has a normal limit of 2.6 ± 1.02%.


1974 ◽  
Vol 13 (02) ◽  
pp. 193-206
Author(s):  
L. Conte ◽  
L. Mombelli ◽  
A. Vanoli

SummaryWe have put forward a method to be used in the field of nuclear medicine, for calculating internally absorbed doses in patients. The simplicity and flexibility of this method allow one to make a rapid estimation of risk both to the individual and to the population. In order to calculate the absorbed doses we based our procedure on the concept of the mean absorbed fraction, taking into account anatomical and functional variability which is highly important in the calculation of internal doses in children. With this aim in mind we prepared tables which take into consideration anatomical differences and which permit the calculation of the mean absorbed doses in the whole body, in the organs accumulating radioactivity, in the gonads and in the marrow; all this for those radionuclides most widely used in nuclear medicine. By comparing our results with dose obtained from the use of M.I.R.D.'s method it can be seen that when the errors inherent in these types of calculation are taken into account, the results of both methods are in close agreement.


2020 ◽  
Vol 27 (16) ◽  
pp. 20554-20564
Author(s):  
Le Thi Bao Ngoc ◽  
Tran Anh Tu ◽  
Luu Thi Thanh Hien ◽  
Duong Nhat Linh ◽  
Nguyen Tri ◽  
...  

2008 ◽  
Vol 68 (5-6) ◽  
pp. 415-419 ◽  
Author(s):  
Wan Aini Wan Ibrahim ◽  
Dadan Hermawan ◽  
Mohamed Noor Hasan ◽  
Hassan Y. Aboul Enein ◽  
M. Marsin Sanagi

2007 ◽  
Vol 20 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Lars Helge Stien ◽  
Anders Kiessling ◽  
Fredrik Manne

1925 ◽  
Vol 13 (1) ◽  
pp. 79-83 ◽  
Author(s):  
Hugh H. Young ◽  
Charles F. Elvers
Keyword(s):  

2015 ◽  
Vol 120 (9) ◽  
pp. 6487-6500 ◽  
Author(s):  
Sebastián Riquelme ◽  
Mauricio Fuentes ◽  
Gavin P. Hayes ◽  
Jaime Campos

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