Small interfering RNA (siRNA) against Slug induces apoptosis and sensitizes human anaplastic thyroid carcinoma cells to doxorubicin

2017 ◽  
Vol 18 (4) ◽  
pp. 357-366 ◽  
Author(s):  
Yinghua Pan ◽  
Peiji Liu ◽  
Deng Chen ◽  
Linying Dou
Keyword(s):  
Thyroid Carcinoma ◽  
Small Interfering Rna ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Interfering Rna

Evaluation of E-Cadherin and β-Catenin Immunoreactivity for Determining Undifferentiated Cells in Anaplastic Thyroid Carcinoma

Pathobiology ◽  
2021 ◽  
pp. 1-8
Author(s):  
Risa Kanematsu ◽  
Mitsuyoshi Hirokawa ◽  
Aki Tanaka ◽  
Ayana Suzuki ◽  
Miyoko Higuchi ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Thyroid Carcinoma ◽  
Undifferentiated Carcinoma ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Membrane Expression ◽  
Thyroid Carcinomas ◽  
Nuclear Expression ◽  
Thyroid Transcription Factor 1 ◽  
E Cadherin

<b><i>Introduction:</i></b> An immunohistochemical study has occasionally been performed to diagnose anaplastic thyroid carcinoma (ATC). However, antibodies to confirm the undifferentiated nature of ATC have not yet been evaluated. The aim of this study was to evaluate E-cadherin and β-catenin expressions in immunoreactivity to determine undifferentiated carcinoma cells in the diagnosis of ATC. <b><i>Methods:</i></b> We immunohistochemically examined 29 ATCs, 30 poorly differentiated thyroid carcinomas (PDTCs), 22 well-differentiated thyroid carcinomas (WDTCs), and 3 squamous cell carcinomas. Antibodies for thyroid transcription factor-1 (TTF-1), paired-box gene 8 (PAX8), β-catenin, and E-cadherin were used. <b><i>Results:</i></b> All WDTCs tested positive for TTF-1, PAX8, and E-cadherin. The positive rates of TTF-1, PAX8, and E-cadherin were 93.3, 93.3, and 100%, respectively, in PDTCs and 17.2, 51.7, and 10.3%, respectively, in ATCs. WDTC expressed the lateral cell membrane staining for β-catenin and E-cadherin, whereas PDTC showed circumferential cell membranous expression (fishnet pattern). β-catenin cell membrane expression in ATCs is lost or discontinuous. Carcinoma cells with β-catenin nuclear expression without cell membranous expression were scattered in 72.4% of ATCs but were not observed in the other carcinomas. <b><i>Conclusion:</i></b> We propose 3 immunohistochemical findings to determine undifferentiated carcinoma cells in the diagnosis of ATC: (1) β-catenin nuclear expression with no or reduced cell membranous expression, (2) the loss or discontinuous pattern of E-cadherin expression, and (3) the loss of PAX8 nuclear expression.

scholarly journals Imatinib enhances docetaxel-induced apoptosis through inhibition of nuclear factor-κB activation in anaplastic thyroid carcinoma cells

Thyroid ◽  
2012 ◽  
pp. 120406132503004
Author(s):  
EunSook Kim ◽  
Michiko Matsuse ◽  
Vladimir Saenko ◽  
Keiji Suzuki ◽  
Akira Ohtsuru ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Nuclear Factor Κb ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Nuclear Factor ◽  
Induced Apoptosis

scholarly journals Anoctamin-1 affects the migration and invasion of anaplastic thyroid carcinoma cells

2019 ◽  
Vol 23 (4) ◽  
pp. 294-301 ◽  
Author(s):  
Jae-Young Kim ◽  
Hwa Young Youn ◽  
June Choi ◽  
Seung Kuk Baek ◽  
Soon Young Kwon ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Migration And Invasion ◽  
Anoctamin 1

scholarly journals Protein arginine N-methyltransferase 1 promotes the proliferation and metastasis of hepatocellular carcinoma cells

Tumor Biology ◽  
2017 ◽  
Vol 39 (2) ◽  
pp. 101042831769141 ◽  
Author(s):  
Qing Gou ◽  
ShuJiao He ◽  
ZeJian Zhou
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Lines ◽  
Small Interfering Rna ◽  
Carcinoma Cell ◽  
Carcinoma Cells ◽  
Hepatocellular Carcinoma Cells ◽  
Carcinoma Cell Lines ◽  
Hepatocellular Carcinoma Cell ◽  
Interfering Rna ◽  
Methyltransferase 1

Hepatocellular carcinoma is the most common subtype of liver cancer. Protein arginine N-methyltransferase 1 was shown to be upregulated in various cancers. However, the role of protein arginine N-methyltransferase 1 in hepatocellular carcinoma progression remains incompletely understood. We investigated the clinical and functional significance of protein arginine N-methyltransferase 1 in a series of clinical hepatocellular carcinoma samples and a panel of hepatocellular carcinoma cell lines. We performed suppression analysis of protein arginine N-methyltransferase 1 using small interfering RNA to determine the biological roles of protein arginine N-methyltransferase 1 in hepatocellular carcinoma. In addition, the expression of epithelial-mesenchymal transition indicators was verified by western blotting in hepatocellular carcinoma cell lines after small interfering RNA treatment. Protein arginine N-methyltransferase 1 expression was found to be significantly upregulated in hepatocellular carcinoma cell lines and clinical tissues. Moreover, downregulation of protein arginine N-methyltransferase 1 in hepatocellular carcinoma cells by small interfering RNA could inhibit cell proliferation, migration, and invasion in vitro. These results indicate that protein arginine N-methyltransferase 1 may contribute to hepatocellular carcinoma progression and serves as a promising target for the treatment of hepatocellular carcinoma patients.

scholarly journals C6-ceramide Inhibits the Motility of Anaplastic Thyroid Carcinoma Cells

2020 ◽  
Vol 63 (2) ◽  
pp. 95-98
Author(s):  
Kazunori Fujiwara ◽  
Hiroaki Yazama ◽  
Ryohei Donishi ◽  
Satoshi Koyama ◽  
Takahiro Fukuhara ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells
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