scholarly journals A novel dendritic nanocarrier of polyamidoamine-polyethylene glycol-cyclic RGD for “smart” small interfering RNA delivery and in vitro antitumor effects by human ether-à-go-go-related gene silencing in anaplastic thyroid carcinoma cells

2013 ◽  
pp. 1293 ◽  
Author(s):  
Guanhua Li ◽  
S Wang ◽  
Hu ◽  
Yin ◽  
Li ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Thyroid Carcinoma ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Related Gene ◽  
Antitumor Effects ◽  
Interfering Rna

scholarly journals Switching the intracellular pathway and enhancing the therapeutic efficacy of small interfering RNA by auroliposome

2020 ◽  
Vol 6 (30) ◽  
pp. eaba5379 ◽  
Author(s):  
Md. Nazir Hossen ◽  
Lin Wang ◽  
Harisha R. Chinthalapally ◽  
Joe D. Robertson ◽  
Kar-Ming Fung ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gold Nanoparticles ◽  
Gene Silencing ◽  
Small Interfering Rna ◽  
Sirna Delivery ◽  
Delivery Systems ◽  
Ovarian Cancer Cells ◽  
Interfering Rna

Gene silencing using small-interfering RNA (siRNA) is a viable therapeutic approach; however, the lack of effective delivery systems limits its clinical translation. Herein, we doped conventional siRNA-liposomal formulations with gold nanoparticles to create “auroliposomes,” which significantly enhanced gene silencing. We targeted MICU1, a novel glycolytic switch in ovarian cancer, and delivered MICU1-siRNA using three delivery systems—commercial transfection agents, conventional liposomes, and auroliposomes. Low-dose siRNA via transfection or conventional liposomes was ineffective for MICU1 silencing; however, in auroliposomes, the same dose gave >85% gene silencing. Efficacy was evident from both in vitro growth assays of ovarian cancer cells and in vivo tumor growth in human ovarian cell line—and patient-derived xenograft models. Incorporation of gold nanoparticles shifted intracellular uptake pathways such that liposomes avoided degradation within lysosomes. Auroliposomes were nontoxic to vital organs. Therefore, auroliposomes represent a novel siRNA delivery system with superior efficacy for multiple therapeutic applications.

miR-199a-5p suppresses epithelial- mesenchymal-transition in anaplastic thyroid carcinoma cells via targeting Snail signals

2020 ◽  
Vol 29 (3) ◽  
pp. 317-326
Author(s):  
Fengyun Hao ◽  
Ya-Nan Bi ◽  
Lei Wang ◽  
Yubing Wang ◽  
Jilei Ma ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Epithelial Mesenchymal Transition ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cells ◽  
Migration And Invasion ◽  
Mesenchymal Transition ◽  
Novel Approach ◽  
Accurate Expression

MicroRNAs (miRNAs) have been validated to play prominent roles in the occurrence and development of anaplastic thyroid carcinoma (ATC). miR-199a-5p was previously reported to act as a tumor suppressor or oncomiRNA in various types of cancer. However, its accurate expression, function, and mechanism in ATC remain unclear. Here, we find that miR-199a-5p is significantly downregulated in ATC tissues compared with adjacent non-cancerous tissues. Overexpression of miR-199a-5p significantly inhibits migration and invasion of ATC cells in vitro, and lung metastasis in vivo. Importantly, miR-199a-5p suppresses epithelial-mesenchymal transition (EMT) both in vitro and in vivo by targeting Snail. Taken together, this study reveals that miR-199a-5p is critical to the EMT progression in ATC cells. Targeting the pathway described here may be a novel approach for inhibiting metastasis of ATC.

An Amphiphilic Dendrimer for Effective Delivery of Small Interfering RNA and Gene Silencing In Vitro and In Vivo

2012 ◽  
Vol 51 (34) ◽  
pp. 8478-8484 ◽  
Author(s):  
Tianzhu Yu ◽  
Xiaoxuan Liu ◽  
Anne-Laure Bolcato-Bellemin ◽  
Yang Wang ◽  
Cheng Liu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Effective Delivery ◽  
Interfering Rna

An Amphiphilic Dendrimer for Effective Delivery of Small Interfering RNA and Gene Silencing In Vitro and In Vivo

2012 ◽  
Vol 124 (34) ◽  
pp. 8606-8612 ◽  
Author(s):  
Tianzhu Yu ◽  
Xiaoxuan Liu ◽  
Anne-Laure Bolcato-Bellemin ◽  
Yang Wang ◽  
Cheng Liu ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Effective Delivery ◽  
Interfering Rna

Delivery of small interfering RNA with a synthetic collagen poly(Pro-Hyp-Gly) for gene silencing in vitro and in vivo

2010 ◽  
Vol 52 (8) ◽  
pp. 693-699 ◽  
Author(s):  
Taro Adachi ◽  
Emi Kawakami ◽  
Naozumi Ishimaru ◽  
Takahiro Ochiya ◽  
Yoshio Hayashi ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Small Interfering Rna ◽  
Interfering Rna
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