Pericardial fluid and vascular tissue engineering: A preliminary study

2021 ◽  
Vol 32 (2) ◽  
pp. 101-113
Author(s):  
Dilek Sönmezer ◽  
Fatma Lati̇foğlu ◽  
Güler Toprak ◽  
Ayhan Düzler ◽  
İsmail Alper İşoğlu
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Mtt Assay ◽  
Vascular Tissue ◽  
Pericardial Fluid ◽  
Suitable Material ◽  
Pericardial Cavity ◽  
Diphenyltetrazolium Bromide ◽  
Fetal Bovine ◽  
Preliminary Study

BACKGROUND: The heart is surrounded by a membrane called pericardium or pericardial cavity. OBJECTIVE: In this study, we investigated the pericardial fluid (PF) for coating polycaprolactone (PCL) scaffolds. PFS, which is a PF component, was used for the coating material. In addition to using PFS for surface coating, MED and fetal bovine serum (FBS) were also used for comparison. METHODS: Pericardial fluid cells (PFSc) isolated from PF were cultured on coated PCL scaffolds for 1, 3, and 5 days. Cell viability was determined using 3-(4, 5-di-methylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. RESULTS: The MTT assay results showed that the viability of cells on PCL scaffold coated with PFS increased over time (P < 0.005), and cell viability was significantly different between PCL scaffolds coated with PFS and non-coated PCL scaffolds. However, cell viability was significantly higher in the PCL scaffolds coated with PFS than non-coated and coated with FBS, MED, and PCL scaffolds. Scanning electron microscopy (SEM) microscopy images and MTT assay indicated that PFSc are attached, proliferated, and spread on PCL scaffolds, especially on PCL scaffolds coated with PFS. CONCLUSIONS: These results suggest that PFS is a biocompatible material for surface modification of PCL scaffolds, which can be used as a suitable material for tissue engineering applications.

scholarly journals Effects of Apigenin, Luteolin, and Quercetin on the Natural Killer (NK-92) Cells Proliferation: A Potential Role as Immunomodulator

2021 ◽  
Vol 50 (3) ◽  
pp. 821-828
Author(s):  
MYO OO@MOHD HASYM AUNG ◽  
NASIR MAT NOR ◽  
LIYANA HAZWANI MOHD ADNAN ◽  
NOR ZIDAH BINTI AHMAD ◽  
ABDI WIRA SEPTAMA ◽  
...  
Keyword(s):  
Cell Viability ◽  
Natural Killer ◽  
Horse Serum ◽  
Interleukin 2 ◽  
Blue Staining ◽  
Anticancer Properties ◽  
Flavonoid Compounds ◽  
Diphenyltetrazolium Bromide ◽  
Recombinant Interleukin 2 ◽  
Fetal Bovine

Cancer can be classified as a fourth leading cause of death in Malaysia. There is a continuous effort by scientists in finding alternative cure to cancer due to the known side effects of chemotherapy and radiation therapy as well as recurrences. One of the latest methods to kill cancerous cells is by using immune cells known as natural killer (NK) cells. Flavonoids such as flavone and flavonol are also known for their antioxidant, anti-inflammatory, immunomodulatory and anticancer properties. This study was carried out to determine the role of flavonoid compounds of apigenin, luteolin, and quercetin to facilitate the growth of NK-92 cells. NK-92 cell line was grown in tissue culture flasks containing α- Minimum Essential Medium (MEM) medium enriched with L-glutamine, 12.5% fetal bovine serum, 12.5% horse serum, 0.2 mM myo-inositol, 0.02 mM folic acid, and 100 - 200 U/mL recombinant interleukin 2 (IL-2). The cell viability was determined via trypan blue staining where the cells were manually counted by a haemocytometer. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability of NK-92 cells after treatment with apigenin, luteolin, and quercetin. Results showed a dose-dependent proliferative effects of apigenin, luteolin, and quercetin on the proliferation of NK-92 cells showing the highest percentage of proliferation at 100 μg/mL for all compounds (*P < 0.05). However, exceeding the dose of 100 μg/mL had resulted in a decline of cell proliferations percentage. Based on these findings, flavonoid compounds comprising apigenin, luteolin and quercetin were able to induce proliferative effects on NK-92 cells.

Decellularized bovine aorta as a promising 3D elastin scaffold for vascular tissue engineering applications

2021 ◽  
Vol 16 (12) ◽  
pp. 1037-1050
Author(s):  
Tahmineh Kazemi ◽  
Ahmad A Mohammadpour ◽  
Maryam M Matin ◽  
Nasser Mahdavi-Shahri ◽  
Hesam Dehghani ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Mtt Assay ◽  
Reverse Transcription ◽  
Dna Content ◽  
Vascular Tissue ◽  
Smooth Muscle Actin ◽  
Cardiovascular Tissue ◽  
Reverse Transcription Pcr ◽  
Cardiovascular Tissue Engineering

Aim: To evaluate the suitability of using aorta elastin scaffold, in combination with human adipose-derived mesenchymal stem cells (hAd-MSCs), as an approach for cardiovascular tissue engineering. Materials & Methods: Human adipose-derived MSCs were seeded on elastin samples of decellularized bovine aorta. The samples were cultured in vitro to investigate the inductive effects of this scaffold on the cells. The results were evaluated using histological, and immunohistochemical methods, as well as MTT assay, DNA content, reverse transcription-PCR and scanning electron microscopy. Results: Histological staining and DNA content confirmed the efficacy of decellularization procedure (82% DNA removal). MTT assay showed the construct’s ability to support cell viability and proliferation. Cell differentiation was confirmed by reverse transcription-PCR and positive immunohistochemistry for alfa smooth muscle actin and von Willebrand. Conclusion: The prepared aortic elastin samples act as a potential scaffold, in combination with MSCs, for applications in cardiovascular tissue engineering. Further experiments in animal models are required to confirm this.

scholarly journals Microplasma-assisted hydrogel fabrication: A novel method for gelatin-graphene oxide nano composite hydrogel synthesis for biomedical application

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3498 ◽  
Author(s):  
Mantosh Kumar Satapathy ◽  
Wei-Hung Chiang ◽  
Er-Yuan Chuang ◽  
Chih-Hwa Chen ◽  
Jia-Liang Liao ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Graphene Oxide ◽  
Cell Viability ◽  
Mtt Assay ◽  
Composite Hydrogel ◽  
Swelling Behavior ◽  
Great Promise ◽  
Cross Linking ◽  
Nano Composite

Toxicity issues and biocompatibility concerns with traditional classical chemical cross-linking processes prevent them from being universal approaches for hydrogel fabrication for tissue engineering. Physical cross-linking methods are non-toxic and widely used to obtain cross-linked polymers in a tunable manner. Therefore, in the current study, argon micro-plasma was introduced as a neutral energy source for cross-linking in fabrication of the desired gelatin-graphene oxide (gel-GO) nanocomposite hydrogel scaffolds. Argon microplasma was used to treat purified gelatin (8% w/v) containing 0.1∼1 wt% of high-functionality nano-graphene oxide (GO). Optimized plasma conditions (2,500 V and 8.7 mA) for 15 min with a gas flow rate of 100 standard cm3/min was found to be most suitable for producing the gel-GO nanocomposite hydrogels. The developed hydrogel was characterized by the degree of cross-linking, FTIR spectroscopy, SEM, confocal microscopy, swelling behavior, contact angle measurement, and rheology. The cell viability was examined by an MTT assay and a live/dead assay. The pore size of the hydrogel was found to be 287 ± 27 µm with a contact angle of 78° ± 3.7°. Rheological data revealed improved storage as well as a loss modulus of up to 50% with tunable viscoelasticity, gel strength, and mechanical properties at 37 °C temperature in the microplasma-treated groups. The swelling behavior demonstrated a better water-holding capacity of the gel-GO hydrogels for cell growth and proliferation. Results of the MTT assay, microscopy, and live/dead assay exhibited better cell viability at 1% (w/w) of high-functionality GO in gelatin. The highlight of the present study is the first successful attempt of microplasma-assisted gelatin-GO nano composite hydrogel fabrication that offers great promise and optimism for further biomedical tissue engineering applications.

scholarly journals Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres

2017 ◽  
Vol 8 (1) ◽  
pp. 28-36
Author(s):  
Ravindra Kotian ◽  
P Prasad Rao ◽  
Prashanthi Madhyastha ◽  
KL Shobha ◽  
BS Satish Rao
Keyword(s):  
Cell Viability ◽  
Mtt Assay ◽  
Platelet Adhesion ◽  
Hydrogen Plasma ◽  
Pure Titanium ◽  
Coated Sample ◽  
Time Intervals ◽  
Diphenyltetrazolium Bromide ◽  
Gas Atmosphere ◽  
Maximum Cell

ABSTRACT Aim This study was performed to evaluate the biocompatibility of pure titanium and Ti-6Al-4V metals coated with hydroxyapatite (HA) by plasma spray using different plasma gas atmospheres. Materials and methods The cell viabilities for each HA-coated sample in an atmosphere of argon, argon—hydrogen, nitrogen, and nitrogen—hydrogen were studied using MTT assay and platelet adhesion test. Results The mean cell viabilities by MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide] assay of samples coated with HA in argon—hydrogen plasma atmosphere showed maximum cell viability at different time intervals compared with other coating atmospheres of argon—hydrogen, nitrogen, and nitrogen—hydrogen. A statistically significant value of cell viability (p < 0.001) was observed between and within the groups of argon, argon—hydrogen, nitrogen, and nitrogen—hydrogen plasma gas atmosphere. The platelet adhesion study showed agglomerates of platelet cells in some isolated regions of HA for all atmospheres. Significance The results obtained in this study can serve as a guide for the development of new Ti-based HA-coated implants in different plasma gas atmospheres. How to cite this article Kotian R, Rao PP, Madhyastha P, Shobha KL, Rao BSS, Ginjupalli K. Cytocompatibility by MTT Assay and Platelet Adhesion of Ti and Ti-6Al-4V coated with Hydroxyapatite in different Plasma Gas Atmospheres. World J Dent 2017;8(1):28-36.

Vascular tissue engineering: Biomechanical studies on autologeous small-calibred vessels

2006 ◽  
Vol 54 (S 1) ◽  
Author(s):  
K Kallenbach ◽  
J Heine ◽  
E Lefik ◽  
S Cebotari ◽  
A Lichtenberg ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering
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