scholarly journals Acerola (Malpighia emarginata DC.) Promotes Ascorbic Acid Uptake into Human Intestinal Caco-2 Cells via Enhancing the Gene Expression of Sodium-Dependent Vitamin C Transporter 1

2020 ◽  
Vol 66 (4) ◽  
pp. 296-299
Author(s):  
Yuka TAKINO ◽  
Hitoshi AOKI ◽  
Yoshitaka KONDO ◽  
Akihito ISHIGAMI
Keyword(s):  
Gene Expression ◽  
Ascorbic Acid ◽  
Vitamin C ◽  
Acid Uptake ◽  
Sodium Dependent ◽  
Malpighia Emarginata

scholarly journals Gene expression profiles of sodium-dependent vitamin C transporters in mice after alcohol consumption

2013 ◽  
Vol 45 (11) ◽  
pp. 912-920 ◽  
Author(s):  
Xiaoqiang Guo ◽  
Yuejia Wang ◽  
Yongqing Shen ◽  
Yingjie Gao ◽  
Yanzhong Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alcohol Consumption ◽  
Vitamin C ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Sodium Dependent

scholarly journals Ascorbic Acid Transported by Sodium-Dependent Vitamin C Transporter 2 Stimulates Steroidogenesis in Human Choriocarcinoma Cells

Endocrinology ◽  
2008 ◽  
Vol 149 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Ximei Wu ◽  
Takuma Iguchi ◽  
Norio Itoh ◽  
Kousuke Okamoto ◽  
Tatsuya Takagi ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Sodium Dependent ◽  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells

scholarly journals Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent

2000 ◽  
Vol 345 (3) ◽  
pp. 665-672 ◽  
Author(s):  
Isabella SAVINI ◽  
Sylvie DUFLOT ◽  
Luciana AVIGLIANO
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Reductase Activity ◽  
Thioredoxin Reductase ◽  
Dehydroascorbic Acid ◽  
Reduction Process ◽  
Enzymic Activity ◽  
Glutathione Depletion ◽  
Acid Uptake ◽  
Hacat Cells

Vitamin C plays an important role in neutralizing toxic free radicals formed during oxidative metabolism or UV exposure of human skin. This study was performed to investigate the mechanisms that regulate the homoeostasis of vitamin C in HaCaT cells by identifying the events involved in the transport and in the reduction of dehydroascorbic acid. Dehydroascorbic acid accumulated to a greater extent and faster compared with ascorbic acid; its transport appeared to be mediated by hexose transporters and was entirely distinct from ascorbic acid transport. Dehydroascorbate reductase activity was unaffected by glutathione depletion, although it was sensitive to thiol protein reagents. These observations, as well as the subcellular distribution of this enzymic activity and the cofactor specificity, indicate that thioredoxin reductase and lipoamide dehydrogenase play an important role in this reduction process. HaCaT cells were able to enhance their dehydroascorbic acid reductase activity in response to oxidative stress.

scholarly journals Iron regulates the uptake of ascorbic acid and the expression of sodium-dependent vitamin C transporter 1 (SVCT1) in human intestinal Caco-2 cells

2011 ◽  
Vol 105 (12) ◽  
pp. 1734-1740 ◽  
Author(s):  
Nathalie M. Scheers ◽  
Ann-Sofie Sandberg
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Cellular Uptake ◽  
Sodium Ascorbate ◽  
Acid Uptake ◽  
Compartment System ◽  
Treatment And Prevention ◽  
Acid Deficiency ◽  
The Impact ◽  
O 10

Ascorbic acid (vitamin C) has major effects on the intestinal uptake and utilisation of Fe in humans. The objective of the present study was to investigate the impact of Fe on the acquisition of ascorbic acid. The strategy was to study the cellular uptake and transport of ascorbic acid in the presence of Fe and also to observe the expression of the Na-dependent vitamin C transporter 1 (SVCT1) protein in human intestinal Caco-2 cells. SVCT1 is involved in the cellular uptake of ascorbic acid and is therefore a candidate for playing a role in the regulation of Fe utilisation. Caco-2 cells were cultured on transmembrane inserts in a three-compartment system followed by treatment with various combinations of FeCl2·4H2O (10–20 μmol/l) and sodium ascorbate (150 μmol/l). ELISA and Western blot analyses revealed that both SVCT1 and ferritin expressions were up-regulated in the presence of ascorbic acid in the basal compartment underneath the cells (10 and 22 %, respectively). Furthermore, when cells deficient in ascorbic acid were exposed to Fe, SVCT1 expression increased significantly (23·7 %). The increase in SVCT1 expression correlated with an increase in ascorbic acid uptake (285 %) in Fe-treated cells, as indicated by the SVCT1 inhibitor quercetin. We conclude that Fe plays an important role in regulating the uptake of ascorbic acid in human intestinal Caco-2 cells. This new angle could change the conceptual thinking of Fe and ascorbic acid utilisation and assist in the treatment and prevention of ascorbic acid-deficiency syndromes such as scurvy.

scholarly journals Temporo-spacial microanatomical distribution of the murine sodium-dependent ascorbic acid transporters Slc23a1 and Slc23a2 in the kidney throughout development

2017 ◽  
Vol 95 (3) ◽  
pp. 421-427 ◽  
Author(s):  
Peter K. Eck ◽  
Christopher Corpe ◽  
Mark A. Levine
Keyword(s):  
Ascorbic Acid ◽  
Expression Patterns ◽  
Cell Types ◽  
Acid Uptake ◽  
Temporal Expression ◽  
Knockout Mouse Model ◽  
Sodium Dependent ◽  
Proximal Tubular

The two membrane transporters Slc23a1 and Slc23a2 mediate ascorbic acid uptake into cells. We recently determined the key role of Slc23a1 in renal re-absorption of ascorbic acid in a knockout mouse model. However, the renal spatial and temporal expression patterns of murine Slc23a1 and Slc23a2 are not defined. This study utilizes database evidence combined with experimental confirmation via in-situ hybridization to define the spatial and temporal expression of Slc23a1 in the murine kidney. Slc23a1 is expressed in the early proximal tubule, but not in its precursors during embryonic development, and exclusive proximal tubular expression persists throughout the animal’s lifetime. In contrast, Slc23a2 is uniformly expressed in metabolic cell types such as stromal cells. The expression patterns appear to be conserved from rodent lineages to humans.

Sodium-dependent vitamin C transporter 2 (SVCT2) is necessary for the uptake of L-ascorbic acid into Schwann cells

Glia ◽  
2009 ◽  
pp. NA-NA ◽  
Author(s):  
Burkhard Gess ◽  
Christina Lohmann ◽  
Hartmut Halfter ◽  
Peter Young
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Schwann Cells ◽  
Sodium Dependent
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