scholarly journals Antimicrobial resistance profiles of common mastitis pathogens on Canadian dairy farms

2012 ◽  
Vol 95 (8) ◽  
pp. 4319-4332 ◽  
Author(s):  
V. Saini ◽  
J.T. McClure ◽  
D. Léger ◽  
G.P. Keefe ◽  
D.T. Scholl ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Farms ◽  
Mastitis Pathogens

scholarly journals Detection of antimicrobial resistance genes of carbapenem-resistant Enterobacteriaceae in Escherichia coli isolated from the water supply of smallholder dairy farms in Saraburi and Maha Sarakham, Thailand

2020 ◽  
Vol 6 (1) ◽  
pp. 1-5
Author(s):  
Natapol Pumipuntu ◽  
Sangkom Pumipuntu
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Water Samples ◽  
Dairy Farms ◽  
Drug Resistant ◽  
E Coli ◽  
Carbapenem Resistant ◽  
Carbapenem Resistant Enterobacteriaceae ◽  
Smallholder Dairy Farms

Background and Aim: The problem of antimicrobial resistance of bacteria in both humans and animals is an important public health concern globally, which is likely to increase, including in Thailand, where carbapenem-resistant Enterobacteriaceae (CRE), such as Escherichia coli, are of particular concern. They are pathogens found in the gastrointestinal tract of humans and other animals as well as in the environment. They may cause opportunistic infection and are often resistant to antibiotics in various fields especially in animal husbandry, such as pets or livestock farms. This study aimed to investigate the occurrence of carbapenem-resistant E. coli from water samples of smallholder dairy farms in Saraburi and Maha Sarakham, Thailand. Materials and Methods: Sixty-four water samples were collected from 32 dairy farms in Kaeng Khoi district, Muak Lek district, and Wang Muang district of Saraburi Province, and Kantharawichai district and Mueang district of Maha Sarakham Province, Thailand. All samples were cultured and isolated for E. coli by biochemical tests. All E. coli isolates were tested for drug susceptibility using imipenem, meropenem, and drug resistance genes of carbapenemases such as blaNDM, blaIMP, and blaOXA48 of drug-resistant E. coli isolates detected by polymerase chain reaction (PCR) technique. Results: A total of 182 E. coli isolates were found (140 and 42 isolates from Saraburi and Maha Sarakham, respectively). Drug sensitivity tests found that two isolates of E. coli from water in Kaeng Khoi were resistant to imipenem; therefore, the incidence of E. coli resistance to carbapenem was 1.43% of Saraburi Province. On the other hand, there was no incidence of drug-resistant E. coli in Maha Sarakham. In addition, the detection of the drug-resistant gene of E. coli in both isolates by PCR showed the expression of blaNDM. Conclusion: This study reports E. coli resistance to antimicrobial drugs on livestock farms. It can be considered to be the first report of E. coli CRE detection in a dairy farm at Saraburi, which should be the subject of further extended study.

scholarly journals Characterization of Staphylococcus aureus Isolates From Cases of Clinical Bovine Mastitis on Large-Scale Chinese Dairy Farms

2020 ◽  
Vol 7 ◽  
Author(s):  
Kangjun Liu ◽  
Luyao Tao ◽  
Jianji Li ◽  
Li Fang ◽  
Luying Cui ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cluster Analysis ◽  
Antimicrobial Resistance ◽  
Large Scale ◽  
Bovine Mastitis ◽  
Dairy Farms ◽  
Clinical Mastitis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Bovine mastitis is a prevalent disease that causes serious economic problems globally in the dairy industry. Staphylococcus aureus is an important pathogen of bovine mastitis. This study was conducted to characterize S. aureus isolates from clinical bovine mastitis cases in large-scale dairy herds in China. S. aureus was isolated from 624 clinical mastitis cases and confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). In total, 62 S. aureus isolates were obtained. Cluster analysis, genetic diversity, quantification of biofilm formation, antimicrobial resistance, and detection of virulence genes were performed on these isolates of S. aureus. Eight isolates harbored the mecA gene and were sensitive to oxacillin. MALDI-TOF MS cluster analysis revealed that the 62 isolates were divided into three major clusters (I, II, III) and eight main groups (A–H) at the distance level of 700. The agr II was the most prevalent (56.5%). The 62 S. aureus isolates were assigned to seven spa types. The most common spa type was t529(58.1%), followed by t2196 (14.5%), t518 (14.5%), t571(6.5%), t034 (3.2%), t2734 (1.6%), and t730 (1.6%). Five STs were identified from seven representative isolates as follows: ST630/CC8, ST97/CC97, ST50, ST398, and ST705. All isolates had the ability to form biofilm. Antimicrobial resistance was most frequently observed to ciprofloxacin (29%), followed by penicillin (24.2%), and streptomycin (9.6%). All isolates harbored the fnbA, clfB (100%), icaA, and icaD genes. This study provides the basis for the development of bovine mastitis prevention program on large-scale dairy farms.

scholarly journals The Mastitis Pathogens Culture Collection

2019 ◽  
Vol 8 (15) ◽  
Author(s):  
Simon Dufour ◽  
Josée Labrie ◽  
Mario Jacques
Keyword(s):  
Mammary Gland ◽  
Dairy Farms ◽  
Culture Collection ◽  
Production Data ◽  
Bacterial Isolates ◽  
Milk Samples ◽  
National Cohort ◽  
Mastitis Pathogens

The Mastitis Pathogen Culture Collection contains more than 16,000 mastitis-causing bacterial isolates from milk samples taken from cow quarters and bulk tanks in a national cohort of 91 dairy farms across Canada over a period of 2 years. These isolates are linked to demographic and production data that were recorded at the mammary gland, cow, and farm levels.

scholarly journals Antimicrobial resistance and genotypic relatedness of environmental staphylococci in semi-extensive dairy farms

2018 ◽  
Vol 6 ◽  
pp. 103-106 ◽  
Author(s):  
Daniel Farias Marinho Monte ◽  
Wellington Dias Lopes Júnior ◽  
Melanie Abley ◽  
Wondwossen Abebe Gebreyes ◽  
Celso José Bruno de Oliveira
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Farms

Antimicrobial Resistance and Class 1 Integrons in Pathogenic Escherichia coli from Dairy Farms

2005 ◽  
Vol 2 (4) ◽  
pp. 348-352 ◽  
Author(s):  
Shelton E. Murinda ◽  
Paul D. Ebner ◽  
Lien T. Nguyen ◽  
Alan G. Mathew ◽  
Stephen P. Oliver
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Dairy Farms ◽  
Class 1 Integrons ◽  
Pathogenic Escherichia Coli ◽  
Class 1

Distribution and Antimicrobial Resistance of Clinical and Subclinical Mastitis Pathogens in Dairy Cows in Rhône-Alpes, France

2010 ◽  
Vol 7 (5) ◽  
pp. 479-487 ◽  
Author(s):  
Marie-Anne Botrel ◽  
Marisa Haenni ◽  
Eric Morignat ◽  
Philippe Sulpice ◽  
Jean-Yves Madec ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Mastitis Pathogens
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