Distribution and Antimicrobial Resistance of Clinical and Subclinical Mastitis Pathogens in Dairy Cows in Rhône-Alpes, France

2010 ◽  
Vol 7 (5) ◽  
pp. 479-487 ◽  
Author(s):  
Marie-Anne Botrel ◽  
Marisa Haenni ◽  
Eric Morignat ◽  
Philippe Sulpice ◽  
Jean-Yves Madec ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Mastitis Pathogens

scholarly journals Staphylococcus aureus isolates from bovine subclinical mastitis in central Ethiopia revealed the presence of antimicrobial resistance and resistant genes

2021 ◽  
Author(s):  
Desiye Tesfaye Tegegne ◽  
Gezahegne Mamo ◽  
Hika Waktole ◽  
Gebrerufael Girmay
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Dairy Cows ◽  
Resistance Genes ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Economic Losses ◽  
Central Ethiopia ◽  
Milk Samples ◽  
Lactating Dairy Cows

Abstract Background: Staphylococcus aureus is one of the predominant causative agents of mastitis disease in dairy herds. Mastitis disease has a negative impact in the economic losses in the dairy sector across the globe. The aims of this study were to determine the prevalence and detect antimicrobial resistance genes in the Staphylococcus aureus isolated from milk samples of subclinical bovine mastitis in Central Ethiopia.Methods: A total of 265 lactating dairy cows from various dairy farms in four different geographical locations were screened by California mastitis test (CMT) for bovine subclinical mastitis. One-hundred thirty CMT positive milk samples were collected and transported to laboratory. Different biochemical tests and polymerase chain reaction (PCR) were used for the identification of S. aureus isolates. Finally, phenotypic and genotypic methods were performed for detection of some antimicrobial resistance patterns and genes (mecA, ermA, ermC, and msrA), respectively. Results: From total of 265 lactating dairy cows screened, 49% (n=130) were positive for bovine subclinical mastitis. One-hundred thirty mastitic milk samples were subjected to bacterial culturing, one hundred (76%) S. aureus isolates were identified based on phenotypic characters. Sixty-eight confirmed S. aureus isolates were obtained using PCR. Of the sixty-eight isolates tested 12 samples were contained the methicillin resistance gene A (mecA). No amplification was observed for the erythromycin resistance genes (ermA, ermC, and msrA). Conclusion: The high resistance of Staphylococcus aureus to commonly used antimicrobials contribute in dairy farms may cause health problems in the community consuming raw milk purchased from these farms.

Phenotypical and Genotypical Antimicrobial Resistance of Coagulase-negative staphylococci Isolated from Cow Mastitis

2016 ◽  
Vol 19 (3) ◽  
pp. 639-646 ◽  
Author(s):  
I. Klimiene ◽  
M. Virgailis ◽  
A. Pavilonis ◽  
R. Siugzdiniene ◽  
R. Mockeliunas ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Dairy Cows ◽  
Resistance Genes ◽  
Subclinical Mastitis ◽  
High Rate ◽  
Antimicrobial Compound ◽  
Coagulase Negative Staphylococci ◽  
Antimicrobial Resistance Genes ◽  
Resistance To Penicillin ◽  
Encoding Genes

AbstractThe objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (CNS) isolated from dairy cows with subclinical mastitis. Antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (EU-CAST). The presence of resistance-encoding genes was detected by multiplex PCR. A total of 191 CNS isolates were obtained. The CNS isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%), and erythromycin (13.7%). CNS isolates (78.0%) were resistant to at least one antimicrobial compound, and 22.0% were multiresistant. The multiresistant isolates were predominantlyStaphylococcus chromogenes(28.6%),Staphylococcus warneri(19%) andStaphylococcus haemolyticus(14.3%). According to MIC pattern data, multiresistant isolates showed the highest resistance (p<0.05) rates to penicillin (85.7%), tetracycline (66.7%), and erythromycin (48.2%), but all of them were sensitive to daptomycin, oxacillin, qiunupristin/dalfopristin, and vancomycin.S. chromogenes (9.5%),S. haemolyticus(4.8%), andS. capitis ss capitis(2.4%) strains were resistant to methicillin; their resistance to oxacillin and penicillin was more than 8 mg/l. A high rate of resistance to penicillin was linked to ablaZ gene found in 66.6% of the isolated multiresistant CNS strains. Resistance to tetracycline via thetetK (38.1%) gene and penicillin via themecA (23.8%) gene were detected less frequently. GenemsrAB was responsible for macrolides and lincosamides resistance and detected in 28.6% of the CNS isolates. Antimicrobial resistance genes were identified more frequently inS. epidermidis,S. chromogenes, andS. warneri.

scholarly journals Formulation and application of nanosilver-infused cream in prevention and treatment of mastitis in dairy cows

2019 ◽  
Vol 41 (2) ◽  
Author(s):  
Nguyen Hoai Chau ◽  
Dao Trong Hien ◽  
Nguyen Thi Thuy ◽  
Doan Quang Ha ◽  
Dang Thi Duong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Treatment Rate ◽  
Environmental Technology ◽  
Long Term Stability ◽  
High Uniformity ◽  
Prevention And Treatment ◽  
Mastitis Pathogens

Research on formulation of nanosilver-infused cream was carried out at the Institute of Environmental Technology and the effects of this cream in prevention and treatment of mastitis in dairy cows were assessed at Bavi Cattle and Forage Research Center. Cream with 0.4% carbomer was infused with silver nanoparticles for high uniformity and long-term stability. At concentrations of 50–250 ppm, nanosilver-infused cream proved to be effective against mastitis pathogens such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. Nanosilver cream at concentrations of 150 ppm had the best effect on clinical and subclinical mastitis prevention in dairy cows. Combining nanosilver cream with ceftiofer antibiotic achieved excellent treatment rate, up to 93.33%. For prevention of dairy mastitis, silver cream can be used at a concentration of 150 ppm 15 days before calving and for the whole milking period. Using nanosilver cream in combination with antibiotics led to highly effective mastitis treatment. 

Prevalence and distribution of mastitis pathogens in subclinically infected dairy cows in Flanders, Belgium

2007 ◽  
Vol 74 (4) ◽  
pp. 478-483 ◽  
Author(s):  
Sofie Piepers ◽  
Luc De Meulemeester ◽  
Aart de Kruif ◽  
Geert Opsomer ◽  
Herman W Barkema ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Geometric Mean ◽  
Dairy Herd ◽  
Subclinical Mastitis ◽  
Cross Sectional ◽  
Intramammary Infections ◽  
Milk Samples ◽  
Lactating Cows ◽  
Culture Positive ◽  
Mastitis Pathogens

The main objective was to determine the prevalence of intramammary infections (IMI) in dairy cows in Flanders, Belgium. Data were obtained from quarter milk samples of dairy herds subjected to a mandatory yearly screening of all lactating cows. A total of 178 668 quarter milk samples were collected at 1087 cross-sectional dairy herd screenings performed in three consecutive years. Of the dairy cows, 40% had at least one culture-positive quarter. More than 50% of all IMI were caused by non-aureus staphylococci. Streptococcus agalactiae is almost eradicated in Flanders, whereas Staphylococcus aureus was isolated from 18% of the culture-positive quarters. In addition, the distribution of mastitis pathogens in quarter milk samples from selected dairy cows with an elevated somatic cell count (SCC) is described. From 6390 cows with a geometric mean composite SCC ⩾250 000 cells/ml, nearly 65% had at least one culture-positive quarter. The majority of the IMI were caused by non-aureus staphylococci (41·1%), whereas Staph. aureus and aesculin-positive cocci were found in respectively 25% and 18% of the culture-positive milk samples. We conclude that more efforts are needed in the prevention and control of subclinical mastitis in Flanders. Non-aureus staphylococci are the predominant cause of IMI, warranting more research regarding the epidemiology and pathogenicity of those species.

Antimicrobial susceptibility of mastitis pathogens of dairy cows in Switzerland

2014 ◽  
Vol 156 (10) ◽  
pp. 483-488 ◽  
Author(s):  
F. Rüegsegger ◽  
J. Ruf ◽  
A. Tschuor ◽  
Y. Sigrist ◽  
M. Rosskopf ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Antimicrobial Susceptibility ◽  
Mastitis Pathogens

Mass-spec-based urinary metabotyping around parturition identifies screening biomarkers for subclinical mastitis in dairy cows

2020 ◽  
Vol 129 ◽  
pp. 39-52
Author(s):  
Grzegorz Zwierzchowski ◽  
Guanshi Zhang ◽  
Rupasri Mandal ◽  
David S. Wishart ◽  
Burim N. Ametaj
Keyword(s):  
Dairy Cows ◽  
Subclinical Mastitis ◽  
Mass Spec

scholarly journals Prevalence of methicillin-resistant Staphylococcus aureus and pattern of antimicrobial resistance in mastitis milk of cattle in Chitwan, Nepal

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Asmita Shrestha ◽  
Rebanta Kumar Bhattarai ◽  
Himal Luitel ◽  
Surendra Karki ◽  
Hom Bahadur Basnet
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Subclinical Mastitis ◽  
World Health ◽  
Dairy Herds ◽  
Microbiological Analysis ◽  
Biochemical Tests ◽  
Methicillin Resistant ◽  
Milk Samples

Abstract Background The threat of methicillin-resistant Staphylococcus aureus (MRSA) exists globally and has been listed as a priority pathogen by the World Health Organization. One of the sources of MRSA emergence is livestock and its products, often raised in poor husbandry conditions. There are limited studies in Nepal to understand the prevalence of MRSA in dairy animals and its antimicrobial resistance (AMR) profile. A cross-sectional study was conducted in Chitwan, one of the major milk-producing districts of Nepal, from February 2018 to September 2019 to estimate the prevalence of MRSA in milk samples and its AMR profile. The collected milk samples (n = 460) were screened using the California Mastitis Test (CMT) and positive samples were subjected to microbiological analysis to isolate and identify S. aureus. Polymerase Chain Reaction (PCR) was used to identify the presence of the mecA gene and screen for MRSA. Results In total, 41.5% (191/460) of milk samples were positive in the CMT test. Out of 191 CMT positive milk samples, the biochemical tests showed that the prevalence of S. aureus was 15.2% (29/191). Among the 29 S. aureus isolates, 6.9% (2/29) were identified as MRSA based on the detection of a mecA gene. This indicates that that 1.05% (2/191) of mastitis milk samples had MRSA. The antibiotic sensitivity test showed that 75.9% (22/29) and 48.3% (14/29) S. aureus isolates were found to be sensitive to Cefazolin and Tetracycline respectively (48.3%), whereas 100% of the isolates were resistant to Ampicillin. In total 96.6% (28/29) of S. aureus isolates were multidrug-resistant (MDR). Conclusions This study revealed a high prevalence of S. aureus-mediated subclinical mastitis in dairy herds in Chitwan, Nepal, with a small proportion of it being MRSA carrying a mecA gene. This S. aureus, CoNS, and MRSA contaminated milk poses a public health risk due to the presence of a phenotype that is resistant to very commonly used antibiotics. It is suggested that dairy herds be screened for subclinical mastitis and treatments for the animals be based on antibiotic susceptibility tests to reduce the prevalence of AMR. Furthermore, future studies should focus on the Staphylococcus spp. to explore the antibiotic resistance genes in addition to the mecA gene to ensure public health.

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