scholarly journals Ultrastructural and Molecular Phylogenetic Identification of the Diplomonad Flagellate Spironucleus salmonis Infecting Hatchery-reared Salmonid Fishes in Hokkaido

2020 ◽  
Vol 55 (1) ◽  
pp. 8-17
Author(s):  
Shinya Mizuno ◽  
Shigehiko Urawa ◽  
Yoshitomo Katsumata ◽  
Takumi Morishita ◽  
Masatoshi Ban
Keyword(s):  
Salmonid Fishes ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

scholarly journals Identifikasi Molekuler Bakteri Endofit Penghasil L-asparaginase yang Diisolasi dari Mangrove Buta-Buta (Excoecaria agallocha)

2019 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Asep Awaludin Prihanto ◽  
Randy Fahrudin Ardiansyah ◽  
Ken Audia Pradarameswari
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Endophytic Bacteria ◽  
Sequence Data ◽  
Enterobacter Cloacae ◽  
Selective Medium ◽  
Mangrove Plant ◽  
Excoecaria Agallocha ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

AbstrakL-asparaginase (EC 3.5.1.1) adalah enzim yang menghidrolisis asam amino L-asparagin menjadi amonia dan asam aspartat. Enzim ini mempunyai manfaat utama dalam bidang farmasi dan industri pangan. Enzim L-asparaginase tersebar secara luas pada mikroorganisme. Mikroorganisme yang mempunyai potensi menghasilkan enzim ini adalah mikroorganisme endofit dari tumbuhan mangrove. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri endofit penghasil L-asparaginase dari tumbuhan mangrove Buta-buta (E. agallocha). Skrining dilakukan dengan menggunakan medium selektif untuk mendapatkan bakteri penghasil enzim L-asparaginase. Identifikasi molekuler dilakukan dengan menggunakan analisis filogenetik berdasarkan data sekuen 16S rDNA. Dari hasil penelitian ini didapatkan lima isolat bakteri endofit penghasil enzim L-asparaginase, di mana isolat penghasil L-asparaginase tertinggi diidentifikasi secara molekuler. Hasil identifikasi filogenetik molekuler menunjukkan bahwa isolat kode D.104 teridentifikasi sebagai Enterobacter cloacae. Molecular Identification of L-asparaginase-Producing Endophytic Bacteria Isolated from Mangrove Buta-Buta (Excoecaria agallocha)AbstractL-asparaginase (EC 3.5.1.1) is an enzyme which hydrolyze amino acid L-asparagine to aspartate and ammonia. Two main applications of this enzyme are in the pharmaceutical and food industries. The enzyme is widely distributed on microorganism. A potential source of L-asparaginase-producing bacteria is an endophytic bacteria from mangrove plant. This study aimed to isolate and identify L-asparaginase-producing endophytic bacteria from a mangrove plant, E. agallocha (Buta-buta). A screening was carried out using a selective medium to obtain the L-asparaginase enzyme producing bacteria. Molecular identification was carried out using phylogenetic analysis based on 16S rDNA sequence data. In this study, five isolates of the L-asparaginase-producing endophytic bacteria were obtained. The molecular phylogenetic identification showed that the highest L-asparaginase-producing bacterial isolate (code D.104) was identified as Enterobacter cloacae.

Molecular phylogenetic identification of the giant freshwater softshell turtle in the Hoan Kiem lake, Hanoi, Vietnam

2017 ◽  
Vol 39 (3) ◽  
pp. 376-381
Author(s):  
Tran Thi Viet Thanh ◽  
Phan Ke Long ◽  
Nguyen Trung Minh ◽  
Nguyen Minh Tam
Keyword(s):  
Phylogenetic Analysis ◽  
Genomic Dna ◽  
Genetic Relationships ◽  
Taxonomic Status ◽  
Turtle Species ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification ◽  
Low Levels ◽  
Softshell Turtle ◽  
Very High

To clarify the taxonomic status of the giant freshwater softshell turtle in the Hoan Kiem Lake (2016), we analyzed four genes COI, COII, Cytb and ND4. Genomic DNA was extracted from the nail tissue. The nucleotide sequences of the four genes were determined with 652bp, 621bp, 993bp and 731bp, respectively and used to analysis of genetic relationships, together with similar sequences published on GenBank belonging to Rafetus swinhoei, Rafetus eupharaticus and Polychelys cantorii. The analysis indicated very low levels of the K2P divergence were found in all the four genes between the turtle species at Hoan Kiem Lake and R. swinhoei (0-3.7%), while very high levels of divergence were observed between the turtle species at Hoan Kiem Lake and R. eupharaticus (8.6-12.8%), and P. cantorii (14.9-25.4%). Phylogenetic analysis showed that the turtle species at Hoan Kiem Lake together with R. swinhoei to form a cluster with strong bootstrap value 100%. The results showed that the turtle species at Hoan Kiem Lake was R. swinhoei. 

Molecular phylogenetic identification of two new brown-rot corticioid fungi in Dacryobolus from southwestern China

Phytotaxa ◽  
2016 ◽  
Vol 265 (2) ◽  
pp. 101 ◽  
Author(s):  
HAI-SHENG YUAN ◽  
YU-HE KAN ◽  
XIAN-ZHEN WAN
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Strong Support ◽  
Brown Rot ◽  
Southwestern China ◽  
Corticioid Fungi ◽  
Chongqing Municipality ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

Two new brown-rot corticioid fungi, Dacryobolus gracilis and D. montanus, are described from Chongqing Municipality and Guangxi Autonomous Region, southwestern China. Dacryobolus gracilis is characterized by fragile, ceraceous basidiocarps, odontioid hymenophore with slender aculei, presence of long aculeal cystidia in the central part of the aculei and narrowly allantoid basidiospores; D. montanus is characterized by resupinate, ceraceous basidiocarps, odontioid hymenophore with thicker aculei, absence of cystidia or cystidioles, and narrowly allantoid basidiospores. Molecular phylogeny inferred from internal transcribed spacer (ITS) and ribosomal large subunit (nLSU) sequences data indicated that these two new species were clustered with the other species of Dacryobolus with a strong support. These two new species are described and illustrated in this paper. A key to known species of Dacryobolus is provided.

Molecular phylogenetic identification of Fasciola flukes in Nepal

2014 ◽  
Vol 63 (6) ◽  
pp. 758-762 ◽  
Author(s):  
Takuya Shoriki ◽  
Madoka Ichikawa-Seki ◽  
Bhuminand Devkota ◽  
Hari B. Rana ◽  
Shiva P. Devkota ◽  
...  
Keyword(s):  
Molecular Phylogenetic ◽  
Phylogenetic Identification
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