Molecular phylogenetic identification of the intestinal anaerobic microbial community in the hindgut of the termite, Reticulitermes speratus , without cultivation

Extremophiles ◽  
1998 ◽  
Vol 2 (3) ◽  
pp. 155-161 ◽  
Author(s):  
T. Kudo ◽  
Moriya Ohkuma ◽  
Shigeharu Moriya ◽  
Satoko Noda ◽  
Kuniyo Ohtoko
Keyword(s):  
Microbial Community ◽  
Reticulitermes Speratus ◽  
Molecular Phylogenetic ◽  
Anaerobic Microbial Community ◽  
Phylogenetic Identification

Aqueous film forming foam and associated perfluoroalkyl substances inhibit methane production and Co-contaminant degradation in an anaerobic microbial community

2019 ◽  
Vol 21 (11) ◽  
pp. 1915-1925 ◽  
Author(s):  
Nicole J. M. Fitzgerald ◽  
Hanna R. Temme ◽  
Matt F. Simcik ◽  
Paige J. Novak
Keyword(s):  
Microbial Community ◽  
Methane Production ◽  
Perfluoroalkyl Substances ◽  
Contaminant Degradation ◽  
Film Forming ◽  
Anaerobic Microbial Community

This work determines the components of aqueous film forming foam responsible for disrupting microbial community functions.

scholarly journals Identifikasi Molekuler Bakteri Endofit Penghasil L-asparaginase yang Diisolasi dari Mangrove Buta-Buta (Excoecaria agallocha)

2019 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Asep Awaludin Prihanto ◽  
Randy Fahrudin Ardiansyah ◽  
Ken Audia Pradarameswari
Keyword(s):  
16S Rdna ◽  
Molecular Identification ◽  
Endophytic Bacteria ◽  
Sequence Data ◽  
Enterobacter Cloacae ◽  
Selective Medium ◽  
Mangrove Plant ◽  
Excoecaria Agallocha ◽  
Molecular Phylogenetic ◽  
Phylogenetic Identification

AbstrakL-asparaginase (EC 3.5.1.1) adalah enzim yang menghidrolisis asam amino L-asparagin menjadi amonia dan asam aspartat. Enzim ini mempunyai manfaat utama dalam bidang farmasi dan industri pangan. Enzim L-asparaginase tersebar secara luas pada mikroorganisme. Mikroorganisme yang mempunyai potensi menghasilkan enzim ini adalah mikroorganisme endofit dari tumbuhan mangrove. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri endofit penghasil L-asparaginase dari tumbuhan mangrove Buta-buta (E. agallocha). Skrining dilakukan dengan menggunakan medium selektif untuk mendapatkan bakteri penghasil enzim L-asparaginase. Identifikasi molekuler dilakukan dengan menggunakan analisis filogenetik berdasarkan data sekuen 16S rDNA. Dari hasil penelitian ini didapatkan lima isolat bakteri endofit penghasil enzim L-asparaginase, di mana isolat penghasil L-asparaginase tertinggi diidentifikasi secara molekuler. Hasil identifikasi filogenetik molekuler menunjukkan bahwa isolat kode D.104 teridentifikasi sebagai Enterobacter cloacae. Molecular Identification of L-asparaginase-Producing Endophytic Bacteria Isolated from Mangrove Buta-Buta (Excoecaria agallocha)AbstractL-asparaginase (EC 3.5.1.1) is an enzyme which hydrolyze amino acid L-asparagine to aspartate and ammonia. Two main applications of this enzyme are in the pharmaceutical and food industries. The enzyme is widely distributed on microorganism. A potential source of L-asparaginase-producing bacteria is an endophytic bacteria from mangrove plant. This study aimed to isolate and identify L-asparaginase-producing endophytic bacteria from a mangrove plant, E. agallocha (Buta-buta). A screening was carried out using a selective medium to obtain the L-asparaginase enzyme producing bacteria. Molecular identification was carried out using phylogenetic analysis based on 16S rDNA sequence data. In this study, five isolates of the L-asparaginase-producing endophytic bacteria were obtained. The molecular phylogenetic identification showed that the highest L-asparaginase-producing bacterial isolate (code D.104) was identified as Enterobacter cloacae.

scholarly journals Influence of Liquid-to-Gas Ratio on the Syngas Fermentation Efficiency: An Experimental Approach

2020 ◽  
Vol 7 (4) ◽  
pp. 138
Author(s):  
Spyridon Achinas ◽  
Sytse Jelmer Mulder ◽  
Gerrit Jan Willem Euverink
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Experimental Approach ◽  
Complete Conversion ◽  
Fed Batch ◽  
Syngas Fermentation ◽  
Fermentation Efficiency ◽  
Anaerobic Microbial Community ◽  
Gas Ratio

Syngas fermentation by methanogens is a novel process to purify biogas. Methanogens are able to ferment non-desirable CO2, H2, and CO to methane. However, to use methanogens on an industrial scale, more research has to be done. There are studies that discuss the growth of methanogens on syngas in combination with acetate. In this research, growth of methanogens on syngas as sole carbon source is discussed. Effluent of an anaerobic fed-batch was selectively cultivated with syngas in 400 mL Eppendorf© bioreactors. After a period of 7 days, fifteen 120 mL flasks were filled with three different liquid-to-gas ratios (1:1, 1:3, 1:5). Results showed that different liquid-to-gas ratios change the metabolic preference of the anaerobic microbial community. Moreover, complete conversion in a four-to-eight-day period, via the carboxidotrophic pathway, was observed in all three liquid-to-gas ratios.

Toxic effect of surfactants and probable products of their biodegradation on methanogenesis in an anaerobic microbial community

Chemosphere ◽  
1999 ◽  
Vol 39 (11) ◽  
pp. 1861-1870 ◽  
Author(s):  
Victoria A Shcherbakova ◽  
Kestutis S Laurinavichius ◽  
Vasily K Akimenko
Keyword(s):  
Microbial Community ◽  
Toxic Effect ◽  
Anaerobic Microbial Community
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