scholarly journals A Selective Medium for Vibrio anguillarum from Cultured Fish and Rapid Identification by Molecular Biological Technique.

1994 ◽  
Vol 29 (2) ◽  
pp. 113-120
Author(s):  
Hideki Kobayashi ◽  
Tetsuo Morozumi ◽  
Tamae Asawa ◽  
Masahito Miyake ◽  
Kenji Mitani ◽  
...  
Keyword(s):  
Vibrio Anguillarum ◽  
Rapid Identification ◽  
Selective Medium ◽  
Molecular Biological Technique ◽  
Cultured Fish ◽  
Biological Technique

scholarly journals High prevalence of unusual genotypes of Toxoplasma gondii infection in pork meat samples from Erechim, Southern Brazil

2007 ◽  
Vol 79 (1) ◽  
pp. 111-114 ◽  
Author(s):  
RN Belfort ◽  
Veronique Nussenblatt ◽  
Luiz Rizzo ◽  
Cristina Muccioli ◽  
Claudio Silveira ◽  
...  
Keyword(s):  
Restriction Analysis ◽  
Rflp Analysis ◽  
Type I ◽  
Type Ii ◽  
Molecular Biological Technique ◽  
High Prevalence ◽  
Biological Technique ◽  
Infectious Uveitis ◽  
Toxoplasma Gondii Infection ◽  
Meat Samples

Toxoplasmosis is the most common cause of infectious uveitis in Brazil, with a higher frequency in the South of the country. We have collected samples from porcine tongue and diaphragm obtained in both large and small abattoirs and used molecular biological technique to determine the prevalence of infection and RFLP analysis to type the parasites. Seventeen out of 50 (34%) samples from the diaphragm and 33 out of 50 (66%) samples from the tongue demonstrated a positive PCR reaction for T. gondii and restriction analysis of four of the positive samples revealed that all had a type I genotype at SAG2. However, when other unlinked loci were analyzed, these strains had a type III genotype at markers BTUB, SAG3, and GRA6. One of the strains (8T) had a type II allele at SAG3, indicating it has a combination of alleles normally seen in the clonal lineages. Our sampling indicates a high prevalence of infection and suggests that unusual genotypes of T. gondii are found in Brazil even among domesticated pigs.

scholarly journals Vibrio anguillarum isolated from diseased cultured fish in Italy.

1987 ◽  
Vol 22 (4) ◽  
pp. 195-200 ◽  
Author(s):  
Fulvio SALATI ◽  
Giuseppe CESCHIA ◽  
Giorgio GIORGETTI ◽  
Riichi KUSUDA
Keyword(s):  
Vibrio Anguillarum ◽  
Cultured Fish

scholarly journals Commercial phenotypic tests (API 20E) in diagnosis of fish bacteria: a review

2008 ◽  
Vol 52 (No. 2) ◽  
pp. 49-53 ◽  
Author(s):  
N. Topic Popovic ◽  
R. Coz-Rakovac ◽  
I. Strunjak-Perovic
Keyword(s):  
Vibrio Anguillarum ◽  
Rapid Identification ◽  
Identification System ◽  
Bacterial Isolates ◽  
Yersinia Ruckeri ◽  
Fish Health ◽  
Fish Pathogens ◽  
Phenotypic Tests ◽  
Fish Bacteria ◽  
Important Fish

The available data concerning rapid identification of fish bacteria via commercial phenotypic tests demonstrate that there is no agreement regarding the choice of the tests. However, API 20E, an identification system for Enterobacteriaceae and other non-fastidious Gram-negative rods developed for clinical specimens, seems to be increasingly used for the identification of fish pathogens. In this review, adaptation of API 20E for fish bacterial isolates and its distinctiveness for fish bacteria was assessed. Some strains are wrongly identified because they are not included in the database of API 20E system. API 20E reactions should be compared with the diagnostic schemes based on reactions in conventional phenotypic tests. Due to their significance for fish health and impact on the aquaculture, and because of the need for their rapid identification, some important fish bacteria should be included in the API 20E system, such as <i>Yersinia ruckeri</i>, <i>Edwardsiella ictaluri</i>, <i>Vibrio anguillarum</i>.

scholarly journals Methane production pathway in biogas reservoirs via molecular biological technique

2012 ◽  
Vol 39 (4) ◽  
pp. 539-546 ◽  
Author(s):  
Xiaofang WEI ◽  
Jishun QIN ◽  
Yanhua SHUAI ◽  
Keyu LIU ◽  
Yijing LUO ◽  
...  
Keyword(s):  
Methane Production ◽  
Molecular Biological Technique ◽  
Biological Technique

scholarly journals Monitoring Harmful Microalgae by Using a Molecular Biological Technique

Food Quality ◽  
10.5772/34251 ◽  
2012 ◽  
Author(s):  
Tomotaka Shiraishi ◽  
Ryoma Kamikawa ◽  
Yoshihiko Sako ◽  
Ichiro Imai
Keyword(s):  
Molecular Biological Technique ◽  
Biological Technique ◽  
Harmful Microalgae

scholarly journals Identification of bacterial pathogens in cultured fish with a custom peptide database constructed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)

2020 ◽  
Author(s):  
Patharapol Piamsomboon ◽  
Janthima Jaresitthikunchai ◽  
Tran Quang Hung ◽  
Sittiruk Roytrakul ◽  
Janenuj Wongtavatchai
Keyword(s):  
Bacterial Pathogens ◽  
Bacterial Species ◽  
Rapid Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Cultured Fish ◽  
Maldi Biotyper ◽  
Tof Ms

Abstract Background: The majority of infectious diseases of cultured fish is caused by bacteria. Rapid identification of bacterial pathogens is necessary for immediate management. The present study developed a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid identification of fish bacterial pathogens. Streptococcus agalactiae, Streptococcus iniae, Aeromonas hydrophila, Aeromonas veronii, and Edwardsiella tarda obtained from diseased fish were used as representative bacterial pathogens in this study. Bacterial peptides were extracted to create a Main Spectra Profile (MSP), and the MSPs of each bacterial species was added into the MALDI Biotyper database. Fifteen additional isolates of each bacterial species were tested to validate the utilized technique. Results: The MSPs of all field isolates were clearly distinguishable, and the MSPs of the same species were clustered together. However, the species identification when matched with the public MALDI Biotyper library (Bruker MALDI Biotyper) showed unreliable results. Accurate identification was only obtained when using the custom-made database, giving a 100% matching result with the reference method. Conclusion: This study demonstrates an alternative technique for effective identification of fish bacterial pathogens. Further applications require a broad, well-established database to accommodate prudent identification of many fish bacterial pathogens by MALDI-TOF MS.

scholarly journals Identification of bacterial pathogens in cultured fish with a custom peptide database constructed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)

2020 ◽  
Author(s):  
Patharapol Piamsomboon ◽  
Janthima Jaresitthikunchai ◽  
Tran Quang Hung ◽  
Sittiruk Roytrakul ◽  
Janenuj Wongtavatchai
Keyword(s):  
Bacterial Pathogens ◽  
Bacterial Species ◽  
Rapid Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Custom Made ◽  
Cultured Fish ◽  
Maldi Biotyper ◽  
Tof Ms

Abstract Background: The majority of infectious diseases of cultured fish is caused by bacteria. Rapid identification of bacterial pathogens is necessary for immediate management. The present study developed a custom Main Spectra Profile (MSP) database and validate the method using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid identification of fish bacterial pathogens. Streptococcus agalactiae, Streptococcus iniae, Aeromonas hydrophila, Aeromonas veronii, and Edwardsiella tarda obtained from diseased fish were used as representative bacterial pathogens in this study. Bacterial peptides were extracted to create a Main Spectra Profile (MSP), and the MSPs of each bacterial species was added into the MALDI Biotyper database. Fifteen additional isolates of each bacterial species were tested to validate the utilized technique. Results: The MSPs of all field isolates were clearly distinguishable, and the MSPs of the same species were clustered together. The identification methodology was validated with 75 bacterial isolates. The reliability and specificity of the method were determined with MALDI Biotyper log score values and matching results with 16s rDNA sequencing. The species identification using the public MALDI Biotyper library (Bruker MALDI Biotyper) showed unreliable results (log score <2.000) with 42.67% matching result with the reference method. In contrast, accurate identification was obtained when using the custom-made database, giving log score > 2.115, and a 100% matching result. Conclusion: This study demonstrates an effective identification of fish bacterial pathogens when a complete custom-made MSP database is applied. Further applications require a broad, well-established database to accommodate prudent identification of many fish bacterial pathogens by MALDI-TOF MS.

scholarly journals A PCR Detection Method for Rapid Identification of Paenibacillus larvae

1999 ◽  
Vol 65 (5) ◽  
pp. 2243-2245 ◽  
Author(s):  
V. A. Govan ◽  
M. H. Allsopp ◽  
S. Davison
Keyword(s):  
Detection Method ◽  
Bacterial Species ◽  
Pcr Primers ◽  
Rapid Identification ◽  
Selective Medium ◽  
Pcr Detection ◽  
Paenibacillus Larvae ◽  
Rrna Gene ◽  
American Foulbrood ◽  
The 16S Rrna Gene

ABSTRACT American foulbrood is a disease of larval honeybees (Apis mellifera) caused by the bacterium Paenibacillus larvae. Over the years attempts have been made to develop a selective medium for the detection of P. larvae spores from honey samples. The most successful of these is a semiselective medium containing nalidixic acid and pipermedic acid. Although this medium allows the growth of P. larvae and prevents the growth of most other bacterial species, the false-positive colonies that grow on it prevent the rapid confirmation of the presence ofP. larvae. Here we describe a PCR detection method which can be used on the colonies that grow on this semiselective medium and thereby allows the rapid confirmation of the presence ofP. larvae. The PCR primers were designed on the basis of the 16S rRNA gene of P. larvae and selectively amplify a 973-bp amplicon. The PCR amplicon was confirmed as originating from P. larvae by sequencing in both directions. Detection was specific for P. larvae, and the primers did not hybridize with DNA from closely related bacterial species.

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