scholarly journals Detection of Renibacterium salmoninarum in tissue sections by different methods - A comparative study with special regard to the indirect immunohistochemical peroxidase technique.

1989 ◽  
Vol 24 (2) ◽  
pp. 101-104 ◽  
Author(s):  
R. W. HOFFMANN ◽  
G. R. BELL ◽  
C. PFEIL-PUTZIEN ◽  
M. OGAWA
Keyword(s):  
Comparative Study ◽  
Renibacterium Salmoninarum ◽  
Special Regard ◽  
Tissue Sections ◽  
Peroxidase Technique

scholarly journals COMPARATIVE STUDY BETWEEN OPTIMAL AND REDUCED FORMALIN-BASED FIXATIVE FOR NORMAL AND PATHOLOGICAL TISSUES

2019 ◽  
pp. 119-122
Author(s):  
SAWSAN SALIH ALHAROON ◽  
NAWAL MUSTAFA ABDULLAH ◽  
NADA HASHIM MOHAMED
Keyword(s):  
Side Effects ◽  
Comparative Study ◽  
Routine Examination ◽  
Laboratory Worker ◽  
Tissue Samples ◽  
Tissue Sections ◽  
Processing Procedure ◽  
Hypotonic Saline ◽  
Harmful Effects ◽  
Pathological Tissues

Objectives: This study aims to use an alternative fixative compound with reduced concentration of formalin to minimize the exposure and related side effects. Methods: Human normal and pathological tissue samples of different organs were immediately immersed in a modified fixative containing formalin, glycerin, ethanol, and hypotonic saline with pH around 7. Two time schedules were used at 8 and 12 h. Tissue samples were undergone routine processing procedure used in histopathological laboratories, scoring of samples was done, tissue sections of new compound fixative were examined for cytoplasmic, nuclear, and architectural properties and compared with 10% neutral buffered formalin (NBF). Results: Tissue samples treated with modified fixative showed good and acceptable preservation of structural, cellular details with less harmful effects when compared with those preserved with routine 10% (NBF). Conclusion: The suitability and effectiveness of reduced formalin-based fixative in histological and histopathological routine examination with minimal harmful effects for laboratory worker were clearly indicated in this study.

scholarly journals An avidin-biotin-peroxidase method for Fc receptors on macrophages isolated from and in sections of rat lung.

1983 ◽  
Vol 31 (9) ◽  
pp. 1139-1141 ◽  
Author(s):  
V M Elner ◽  
A J Hass ◽  
H R Davis ◽  
S Glagov
Keyword(s):  
Immunoglobulin G ◽  
Fc Receptors ◽  
Rat Lung ◽  
Isolated Cells ◽  
Tissue Sections ◽  
Rabbit Immunoglobulin ◽  
Pulmonary Macrophages ◽  
Peroxidase Technique

Receptors for the Fc region of immunoglobulin G (Fc receptors) were detected on pulmonary macrophages by adapting an avidin-biotin-peroxidase technique to isolated cells and sections of rat lung. After incubation with soluble rabbit immunoglobulin G (IgG), surface bound IgG was identified consistently and reproducibly on glass-adherent pulmonary macrophages and on macrophages in tissue sections made from incubated lung slices. Control experiments indicated that binding was specifically mediated by surface Fc receptors. This method may be useful for identifying macrophages in intact tissues.

Immunophenotyping of cutaneous lymphoid infiltrates in frozen and paraffin-embedded tissue sections: A comparative study

1990 ◽  
Vol 22 (3) ◽  
pp. 405-413 ◽  
Author(s):  
Lorenzo Cerroni ◽  
Josef Smolle ◽  
H. Peter Soyer ◽  
Antonio Martinez Aparicio ◽  
Helmut Kerl
Keyword(s):  
Comparative Study ◽  
Tissue Sections ◽  
Lymphoid Infiltrates

scholarly journals Comparative study of spatial localization of HER-2 and CEP17 signals and of HER-2/CEP17 ratios, in “thin” and “thick” tissue sections

The Breast ◽  
2012 ◽  
Vol 21 (1) ◽  
pp. 34-39 ◽  
Author(s):  
E. Kouvaras ◽  
C.N. Papandreou ◽  
D.D. Daliani ◽  
A. Athanasiadis ◽  
G.K. Koukoulis
Keyword(s):  
Comparative Study ◽  
Spatial Localization ◽  
Tissue Sections ◽  
Her 2
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