scholarly journals Simultaneous removal of estrogens and pathogens from secondary treated wastewater by solar photocatalytic treatment

2014 ◽  
Vol 16 (3) ◽  
pp. 543-552 ◽  
Keyword(s):  
Solar Radiation ◽  
Estrogenic Activity ◽  
Treated Wastewater ◽  
Simultaneous Removal ◽  
E Coli ◽  
Water Matrix ◽  
Treated Effluent ◽  
Emerging Compounds ◽  
Wastewater Disinfection ◽  
Water And Wastewater

<p>Recently, the fate of emerging compounds in environmentally relevant samples has attracted considerable attention. Solar semiconductor photocatalysis may offer an appealing methodology to treat such contaminants. At the same time the use of solar photocatalysis for water and wastewater disinfection is a topic well-documented in the literature. In this respect, the simultaneous degradation of synthetic estrogen 17α-ethynylestradiol (EE2) and <em>Escherichia coli </em>removal employing simulated solar radiation and TiO<sub>2</sub> as the photocatalyst was investigated. In general, the more complex the water matrix is the slower <em>E. coli</em> removal becomes, while the presence of <em>E. coli</em> in the reaction mixture did not obstruct EE2 removal.</p> <div> <p>Although EE2 removal occurred relatively fast, overall estrogenic activity was only partially removed. This implies that other species inherently present in the effluent and/or some photocatalytic transformation by-products may be proportionately more estrogenic than EE2. Overall, the use of solar radiation can constitute an advantageous treatment strategy for the simultaneous removal of micro-pollutants and pathogens from secondary treated effluent.</p> </div> <p>&nbsp;</p>

scholarly journals Photoelectrocatalytic disinfection of water and wastewater: performance evaluation by qPCR and culture techniques

2012 ◽  
Vol 11 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Danae Venieri ◽  
Efthalia Chatzisymeon ◽  
Eleonora Politi ◽  
Spiridon S. Sofianos ◽  
Alexandros Katsaounis ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Charge Carriers ◽  
Treated Wastewater ◽  
Bacterial Inactivation ◽  
Culture Techniques ◽  
E Coli ◽  
Log Reduction ◽  
Water Matrix ◽  
Water And Wastewater ◽  
Polymerase Chain

Photoelectrocatalytic oxidation (PEC) was evaluated as a disinfection technique using water and secondary treated wastewater spiked with Escherichia coli and Enterococcus faecalis. PEC experiments were carried out using a TiO2/Ti-film anode and a zirconium cathode under simulated solar radiation. Bacterial inactivation was monitored by culture and quantitative polymerase chain reaction (qPCR). Inactivation rates were enhanced when the duration of the treatment was prolonged and when the bacterial density and the complexity of the water matrix were decreased. E. coli cells were reduced by approximately 6 orders of magnitude after 15 min of PEC treatment in water at 2V of applied potential and an initial concentration of 107 CFU/mL; pure photocatalysis (PC) led to about 5 log reduction, while electrochemical oxidation alone resulted in negligible inactivation. The superiority of PEC relative to PC can be attributed to a more efficient separation of the photogenerated charge carriers. Regarding disinfection in mixed bacterial suspensions, E. coli was more susceptible than E. faecalis at a potential of 2V. The complex composition of wastewater affected disinfection efficiency, yielding lower inactivation rates compared to water treatment. qPCR yielded lower inactivation rates at longer treatment times than culture techniques, presumably due to the fact that the latter do not take into account the viable but not culturable state of microorganisms.

A pilot-scale study of peracetic acid and ultraviolet light for wastewater disinfection

2019 ◽  
Vol 5 (8) ◽  
pp. 1453-1463 ◽  
Author(s):  
Abdulrahman H. Hassaballah ◽  
Jeremy Nyitrai ◽  
Christine H. Hart ◽  
Ning Dai ◽  
Lauren M. Sassoubre
Keyword(s):  
Ultraviolet Light ◽  
Peracetic Acid ◽  
Pilot Scale ◽  
Fecal Coliforms ◽  
Treated Wastewater ◽  
E Coli ◽  
Wastewater Disinfection ◽  
Somatic Coliphage

At the pilot-scale, peracetic acid effectively inactivates fecal coliforms,E. coliandEnterococcusspp. in secondary and tertiary treated wastewater. The addition of UV to PAA treatment increases inactivation of somatic coliphage.

Inactivation of Cryptosporidium parvum oocysts and other microbes in water and wastewater by electrochemically generated mixed oxidants

2000 ◽  
Vol 41 (7) ◽  
pp. 127-134 ◽  
Author(s):  
M. J. Casteel ◽  
M. D. Sobsey ◽  
M. J. Arrowood
Keyword(s):  
Escherichia Coli ◽  
Cell Culture ◽  
Cryptosporidium Parvum ◽  
Propidium Iodide ◽  
Treated Wastewater ◽  
E Coli ◽  
Water And Wastewater ◽  
Cryptosporidium Parvum Oocysts ◽  
Cell Culture Assay ◽  
Escherichia Coli B

Alternative disinfectants of water and wastewater are needed because conventional chlorination is ineffective against C. parvum oocysts. Reliable indicators of disinfection efficacy against C. parvum also are needed. Mixedoxidants (MO) electrochemically generated from brine were evaluated in batch disinfection experiments for inactivation of C. parvum oocysts and Cl. perfringensspores in both oxidant demand-free (ODF) water and treated wastewater. Coliphage MS2 and Escherichia coli B were also tested under some conditions. C. parvum oocyst infectivity was quantified by cell culture assay, and the dyes DAPI (4′,6-diamidino-2-phenylindole) and propidium iodide (PI) were used to assess oocyst viability in wastewater experiments. In treated wastewater dosed with 10–13 mg/L MO, inactivation after 90 minutes was about 3 log10 for C. parvum and about 2.5 log10 for Cl. perfringens spores; MS2 and E. coli were rapidly inactivated by &gt; 5 log10. In ODF water, a 4 mg/L dose of MO inactivated ∼3 log10 of C. parvum oocysts and ∼1.5 log10 of Cl. perfringens spores. Inactivation of C. parvum oocysts and Cl. perfringensspores was less extensive at a lower MO dose of 2 mg/L. The use of DAPI and PI to determine viability of oocysts treated with MO did not correlate with, and greatly overestimated, cell culture infectivity. At practical doses and contact times, MO disinfection of water and wastewater achieves appreciable inactivation of both C. parvum oocysts and Cl. perfringens spores. Cl. perfringens spores reliably indicated oocyst inactivation by MO, but E. coli and coliphage MS2 were inactivated much too rapidly to indicate C. parvum inactivation.

scholarly journals Disinfection of treated wastewater as an essential purification step for safe urban reuse: a comparative pilot study of UV- and ClO2-disinfection systems for urban reuse applications in China

2013 ◽  
Vol 3 (3) ◽  
pp. 325-335 ◽  
Author(s):  
A. Bischoff ◽  
J. H. Fan ◽  
P. Cornel ◽  
M. Wagner ◽  
L. M. Ma
Keyword(s):  
Uv Irradiation ◽  
Water Reuse ◽  
Municipal Wastewater ◽  
Treated Wastewater ◽  
Urban Water ◽  
Log10 Reduction ◽  
Potential Health ◽  
E Coli ◽  
Disinfection Methods ◽  
Wastewater Disinfection

Disinfection of wastewater is vital in addressing the potential health risks of urban water reuse. To compare the applicability of wastewater disinfection methods other than chlorine, identical pilot plants that used ultraviolet (UV) irradiation and chlorine dioxide (ClO2) dosing were installed at municipal wastewater treatment plants in Shanghai, China and Darmstadt, Germany. The investigation included public health and environmental aspects associated with the two disinfection methods. The results of the pilot-scale studies suggest that, in order to comply with Chinese water quality standards for urban water reuse, it is advisable to have a tertiary treatment before wastewater disinfection. Both methods were able to achieve a 4 log10 reduction in both total coliforms and Escherichia coli (E. coli). There was no evidence for regrowth of E. coli. However, after an initial 3 log10 reduction, HPC increased within 48 h by more than 10-fold after UV irradiation as well as after low doses of ClO2. An increase in acute toxicity was detected after dosing with ClO2 but not after UV irradiation.

scholarly journals Water and Wastewater Disinfection with Peracetic Acid and UV Radiation and Using Advanced Oxidative Process PAA/UV

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Jeanette Beber de Souza ◽  
Fernanda Queiroz Valdez ◽  
Rhuan Felipe Jeranoski ◽  
Carlos Magno de Sousa Vidal ◽  
Grasiele Soares Cavallini
Keyword(s):  
Uv Radiation ◽  
Peracetic Acid ◽  
Combined Method ◽  
Experimental Conditions ◽  
E Coli ◽  
Wastewater Disinfection ◽  
Combined Action ◽  
Oxidative Processes ◽  
Water And Wastewater ◽  
The Individual

The individual methods of disinfection peracetic acid (PAA) and UV radiation and combined process PAA/UV in water (synthetic) and sanitary wastewater were employed to verify the individual and combined action of these advanced oxidative processes on the effectiveness of inactivation of microorganisms indicators of fecal contaminationE. coli, total coliforms (in the case of sanitary wastewater), and coliphages (such as virus indicators). Under the experimental conditions investigated, doses of 2, 3, and 4 mg/L of PAA and contact time of 10 minutes and 60 and 90 s exposure to UV radiation, the results indicated that the combined method PAA/UV provided superior efficacy when compared to individual methods of disinfection.

scholarly journals Ultrafiltration Process in Disinfection and Advanced Treatment of Tertiary Treated Wastewater

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 221
Author(s):  
Rafał Tytus Bray ◽  
Katarzyna Jankowska ◽  
Eliza Kulbat ◽  
Aneta Łuczkiewicz ◽  
Aleksandra Sokołowska
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Microscopic Analysis ◽  
Fecal Coliforms ◽  
Treated Wastewater ◽  
Chemical Parameters ◽  
Virus Particles ◽  
E Coli ◽  
The One ◽  
Physical And Chemical

The paper presents the results of research on the use of ultrafiltration, using membranes of 200 and 400 kDa separation, for disinfection of municipal treated wastewater. The research was conducted on a fractional technical scale using real municipal treated wastewater from two large wastewater treatment plants treating most of the wastewater over the one-million polycentric Gdańsk agglomeration (1.2 million inhabitants). UF 200 kDa and UF 400 kDa processes enabled further improvement of the physical and chemical parameters of treated wastewater. Total phosphorus (to below 0.2 mg/L–UF 200 kDa, 0.13 mg/L–UF 400 kDa) and turbid substances (to below 0.2 mg/L, both membranes) were removed in the highest degree. COD was reduced efficiently (to below 25.6 mgO2/L–UF 200 kDa, 26.8 mgO2/L–UF 400 kDa), while total nitrogen was removed to a small extent (to 7.12 mg/L–UF 200 kDa and 5.7 mg/L–UF 400 kDa. Based on the reduction of indicator bacteria; fecal coliforms including E. coli (FC) and fecal enterococci (FE) it was found that the ultrafiltration is an effective method of disinfection. Not much indicator bacterial were observed in the permeate after processes (UF 200 kDa; FC—5 CFU/L; FE—1 CFU/L and UF 400 kDa; FC—70 CFU/L; FE—10 CFU/L. However, microscopic analysis of prokaryotic cells and virus particles showed their presence after the application of both membrane types; TCN 3.0 × 102 cells/mL–UF 200 kDa, 5.0 × 103 cells/mL–UF 400 kDa, VP 1.0 × 105/mL. The presence of potentially pathogenic, highly infectious virus particles means that ultrafiltration cannot be considered a sufficient disinfection method for treated wastewater diverted for reuse or discharged from high load wastewater treatment plants to recreational areas. For full microbiological safety it would be advisable to apply an additional disinfection method (e.g., ozonation).

Urban wastewater disinfection for agricultural reuse: effect of solar driven AOPs in the inactivation of a multidrug resistant E. coli strain

2015 ◽  
Vol 178 ◽  
pp. 65-73 ◽  
Author(s):  
Giovanna Ferro ◽  
Antonino Fiorentino ◽  
María Castro Alferez ◽  
M. Inmaculada Polo-López ◽  
Luigi Rizzo ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Coli Strain ◽  
Urban Wastewater ◽  
E Coli ◽  
Wastewater Disinfection

Diversity of fecal coliforms and their antimicrobial resistance patterns in wastewater treatment model plant

2010 ◽  
Vol 61 (6) ◽  
pp. 1383-1392 ◽  
Author(s):  
A. Łuczkiewicz ◽  
S. Fudala-Książek ◽  
K. Jankowska ◽  
B. Quant ◽  
K. Olańczuk-Neyman
Keyword(s):  
Wastewater Treatment ◽  
Antimicrobial Agents ◽  
Selective Medium ◽  
Fecal Coliforms ◽  
Treated Wastewater ◽  
Treatment Model ◽  
E Coli ◽  
Model Plant ◽  
Resistance Patterns ◽  
Activated Sludge Processes

The occurrence of resistance patterns among wastewater fecal coliforms was determined in the study. Susceptibility of the isolates was tested against 19 antimicrobial agents: aminoglycosides, aztreonam, carbapenems, cephalosporines, β-lactam/β-lactamase inhibitors, penicillines, tetracycline, trimethoprim/sulfamethoxazole, and fluoroquinolones. Additionally the removal of resistant isolates was evaluated in the laboratory-scale wastewater treatment model plant (M-WWTP), continuously supplied with the wastewater obtained from the full-scale WWTP. Number of fecal coliforms in raw (after mechanical treatment) and treated wastewater, as well as in aerobic chamber effluent was determined using selective medium. The selected strains were identified and examined for antibiotic resistance using Phoenix Automated Microbiology System (BD Biosciences, USA). The strains were identified as Escherichia coli (n = 222), Klebsiella pneumoniae ssp. ozaenae (n = 9), and Pantoea agglomerans (n = 1). The isolate of P. agglomerans as well as 48% of E. coli isolates were sensitive to all antimicrobials tested. The most frequent resistance patterns were found for ampicillin: 100% of K. pneumoniae ssp. ozaenae and 41% of E. coli isolates. Among E. coli isolates 12% was regarded as multiple antimicrobial resistant (MAR). In the studied M-WWTP, the applied activated sludge processes reduced considerably the number of fecal coliforms, but increased the ratio of antimicrobial-resistant E. coli isolates to sensitive ones, especially among strains with MAR patterns.

Wastewater disinfection by neutral pH photo-Fenton: The role of solar radiation intensity

2016 ◽  
Vol 181 ◽  
pp. 1-6 ◽  
Author(s):  
E. Ortega-Gómez ◽  
M.M. Ballesteros Martín ◽  
B. Esteban García ◽  
J.A. Sánchez Pérez ◽  
P. Fernández Ibáñez
Keyword(s):  
Solar Radiation ◽  
Radiation Intensity ◽  
Neutral Ph ◽  
Solar Radiation Intensity ◽  
Wastewater Disinfection

scholarly journals Biological Activity of Wastewater Assessed Using in Vitro Cell-Based Assays

2021 ◽  
Author(s):  
Adamo R. Petosa ◽  
Monica Nowierski ◽  
Viviane Yargeau
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Solid Phase ◽  
Treated Wastewater ◽  
Chemical Analyses ◽  
Untreated Wastewater ◽  
17Β Estradiol ◽  
Receiving Waters

Abstract Bioanalytical tools, namely in vitro bioassays, can be employed in tandem with chemical analyses to assess the efficacy of wastewater treatment and the potential for adverse effects from the discharges of wastewater into receiving waters. In the present study, samples of untreated wastewater (i.e. influent) and treated wastewater (i.e. effluent) were collected from two wastewater treatment plants and a wastewater treatment lagoon serving municipalities in southern Ontario, Canada. In addition, grab samples of surface water were collected downstream of the lagoon discharge. After solid phase extraction (SPE) using ion-exchange columns for basic/neutral and acidic compounds, respectively, the extracts were analyzed for a suite of 16 indicator compounds. The two SPE extracts were combined for analysis of biological responses in four in vitro cell-based bioassays. The concentrations of several indicator compounds, including the estrogens, 17β-estradiol and 17α-ethinylestradiol, were below the limits of detection. However, androstenedione and estrone were detected in several influent samples. The concentrations of these steroid hormones and some of the other indicator compounds declined during treatment but acesulfame K, carbamazepine, trimethoprim and DEET persisted in the effluent. The MTS- CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) indicated that cell viability was not affected by exposure to the extracts. The Qiagen Nuclear Receptors 10-Pathway Reporter Array indicated that several cellular pathways were upregulated, with the greatest upregulation observed with the estrogen receptor (i.e. induction ratios 12 to 47) and the liver X receptor (i.e. induction ratios 10 to 45). The ERα CALUX assay indicated that estrogenic activity was lower in effluents compared to influents, with the greatest estrogenic activity observed for grab samples of influent from the lagoon (i.e. 56-215 ng L-1 17β-estradiol equivalents). Finally, the results of the Nrf2 Luciferase Luminescence Assay indicated a lower oxidative stress in the effluent samples. Overall, the present study demonstrates that chemical analyses are limited in their ability to predict or explain reductions in the toxicity of treated wastewater. There are thus advantages to using a combination of chemical analyses and in vitro bioassays to monitor the treatment efficiency of wastewater treatment plants and to predict the potential impacts of wastewater discharges into receiving waters.

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