scholarly journals Effect of intraperitoneal and systemic sirolimus administration on postoperative peritoneal adhesions in rats

2021 ◽  
Author(s):  
Abdullah İNAL ◽  
Mehmet SAYDAM
Keyword(s):  
Peritoneal Adhesions

Effects of methylene blue, indigo carmine solution and autologous erythrocyte suspension on formation of adhesions after injection into rats

Reproduction ◽  
2000 ◽  
pp. 225-229 ◽  
Author(s):  
A Gul ◽  
C Kotan ◽  
I Dilek ◽  
T Gul ◽  
A Tas ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Macrophage Activation ◽  
Indigo Carmine ◽  
Adhesion Formation ◽  
Albino Rats ◽  
Erythrocyte Suspension ◽  
Peritoneal Adhesions ◽  
Tubal Patency ◽  
Macrophage Activity ◽  
Group Score

The aim of this study was to determine whether autologous erythrocyte suspension can be used as a dye for evaluation of tubal patency and whether it has any advantages over methylene blue or indigo carmine solutions. Reproductively healthy female nulliparous Wistar Albino rats (n = 30), aged 6 months, mass 165-195 g, were assigned randomly to three groups. Rats received a 1 ml i.p. injection of 5% (w/v) methylene blue solution (methylene blue group: n = 10), 5% (w/v) indigo carmine solution (indigo carmine group: n = 10) or 5% (v/v) fresh autologous erythrocyte suspension (autologous erythrocyte group: n = 10). At 4 weeks after injection, a small sterile opening was made in the peritoneal cavity of each rat. The cavity was rinsed once with TCM-199 to collect macrophages. The rinsed peritoneal contents were cultured overnight to evaluate macrophage activation. The peritoneal opening was expanded for evaluation of adhesion formation. Only one rat from the autologous erythrocyte group had intra-peritoneal adhesions (score 2), whereas all rats in the methylene blue group (score 1: n = 1; score 2: n = 4; score 3: n = 4; and score 4: n = 1) and seven rats in the indigo carmine group (score 1: n = 1; score 2: n = 2; score 3: n = 3; and score 4: n = 1) had intra-abdominal adhesions. Macrophage activity was observed in the cultured peritoneal contents collected from the methylene blue and indigo carmine groups but not from the autologous erythrocyte group. Adhesion formation could be due to macrophage activation caused by methylene blue and indigo carmine solutions. These results indicate that tubal patency can be observed by laparoscopy using autologous erythrocyte suspension. The results of this study are believed to be the first to indicate that a patient's own erythrocyte suspension could be used during observation of tubal patency by laparoscopy. However, further studies are required.

Peritoneal regeneration and pathogenesis of postoperative peritoneal adhesions formation

2019 ◽  
Vol 0 (3) ◽  
pp. 88-93
Author(s):  
M. G. Melnichenko ◽  
A. A. Kvashnina
Keyword(s):  
Peritoneal Adhesions

A nano chitosan membrane barrier prepared via Nanospider technology with non-toxic solvent for peritoneal adhesions’ prevention

2021 ◽  
pp. 088532822110081
Author(s):  
Shuo Zhang ◽  
Zhuoyue Xu ◽  
Xuejun Wen ◽  
Changzheng Wei
Keyword(s):  
Mouse Fibroblast ◽  
Electrospun Membrane ◽  
Adhesion Barrier ◽  
Peritoneal Adhesions ◽  
Elongation At Break ◽  
In Vivo Experiments ◽  
L929 Mouse Fibroblast ◽  
Membrane Barrier ◽  
L929 Mouse

Peritoneal adhesion is one of the most common postsurgical complications and can cause bowel obstruction, pelvic pain, and infertility. Setting up a physical barrier directly between the injured site and surrounding tissues is an effective solution for preventing this adverse situation. This study investigated a chitosan electrospun membrane (CSEM) as a potent anti-adhesion barrier, which was prepared by a needleless technology called Nanospider. Scanning electron microscopy revealed that CSEM is a laminated nanofiber with good mechanical properties. The fiber is uniform with the diameter distributing in the range of 100–120 nm. The tensile strength can reach 27.45 ± 6.30 MPa with a maximum elongation at break of 18.50 ± 1.44%, which makes it stick easily to damaged parts but not to be easily damaged by tissue friction. The growth of S. aureus on CSEM was 59.18% lower than the control at 10 h, which indicates its better antibacterial property. In addition, CSEM has good coagulant and biocompatibility characteristics. It can perform hemostatic function within 10 min and the L929 mouse fibroblast viability on it was 92.18% ± 1.08% on the seventh day. In vivo experiments indicated that CSEM significantly prevented peritoneal adhesions within four weeks after surgery with wound surface coverage. These results indicate that CSEM is a promising anti-adhesion barrier material.

scholarly journals Appendectomy and women’s reproductive outcomes: a review of the literature

2021 ◽  
Author(s):  
Lucia Merlino ◽  
Alessandra Chiné ◽  
Giulia Carletti ◽  
Federica Del Prete ◽  
Massimo Codacci Pisanelli ◽  
...  
Keyword(s):  
Large Population ◽  
Fluid Secretion ◽  
Reproductive Period ◽  
Pelvic Surgery ◽  
Tubal Infertility ◽  
Peritoneal Adhesions ◽  
Relevant Risk Factor ◽  
Perforated Appendix ◽  
Embryo Transport ◽  
Possible Cause

Summary Background The most frequent abdominal surgery performed for benign disease in females of fertile age is appendectomy, which remains among the most common surgeries and is a possible cause of peritoneal adhesions. The fact that appendectomy can cause adhesions may lead one to think that this may be a relevant risk factor for infertility; however, there is no universal agreement regarding the association between appendectomy and fertility. The aim of this review is to evaluate weather appendectomy may have a relevant impact on female fertility. Methods The search was conducted in PubMed and there was no limitation set on the date of publication. All studies regarding populations of female patients who had undergone appendectomy for inflamed appendix, perforated appendix, or negative appendix between childhood and the end of the reproductive period were included. Results Some authors believe that pelvic surgery can cause adhesions which can potentially lead to tubal infertility by causing tubal obstruction or by altering motility of fimbriae, tubal fluid secretion, and embryo transport. On the other hand, the most recent evidence reported that removal of the appendix seems to be associated with an increased pregnancy rate in large population studies. Conclusion Despite the existence of contrasting opinions concerning fertility after appendectomy, the most recent evidence suggests that appendectomy may actually lead to improved fertility and decreased time to pregnancy. Appendectomy seems to be correlated with improved fertility and higher pregnancy rates.

scholarly journals Plasma Levels of t-PA and PAI-1 Correlate With the Formation of Experimental Post-Surgical Peritoneal Adhesions

2006 ◽  
Vol 2006 ◽  
pp. 1-4 ◽  
Author(s):  
Clara Di Filippo ◽  
Alessandro Falsetto ◽  
Vito De Pascale ◽  
Elisabetta Tufariello ◽  
Domenico De Lucia ◽  
...  
Keyword(s):  
Plasminogen Activator ◽  
Adhesion Formation ◽  
Plasminogen Activator Inhibitor ◽  
Lavage Fluid ◽  
Plasminogen Activator Inhibitor 1 ◽  
Peritoneal Adhesion ◽  
Peritoneal Adhesions ◽  
Risk Patients ◽  
Activator Inhibitor ◽  
Pai 1

This study has evaluated whether systemic changes of plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) parallel the adhesions development and whether they could be used as predictors of adhesion risk. This has been studied in an animal model of post-surgical peritoneal adhesion by monitoring for 10 days the plasma and tissue levels of t-PA and PAI-1. The results showed that both tissular and plasmatic levels of t-PA were decreased in concomitance with the development of peritoneal adhesions. In contrast, PAI-1 was found increased into the tissue and into the plasma samples of the rats taken at 5 and 10 days time points. Inflammatory mediators such as ICAM-1, VCAM-1, and IL-6 within the peritoneal lavage fluid also correlated with the adhesion formation process. In conclusion, post-surgical peritoneal adhesions provide alterations of local inflammatory components and local and systemic fibrinolytic components, possibly with PAI-1 quenching t-PA. This may have potential for the identification of high-risk patients.

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