Peritoneal Adhesions to Prosthetic Materials: An Experimental Comparative Study of Treated and Untreated Polypropylene Meshes Placed in the Abdominal Cavity

2009 ◽  
Vol 19 (3) ◽  
pp. 369-374 ◽  
Author(s):  
Luca Ansaloni ◽  
Fausto Catena ◽  
Federico Coccolini ◽  
Milena Fini ◽  
Filippo Gazzotti ◽  
...  
Keyword(s):  
Comparative Study ◽  
Abdominal Cavity ◽  
Peritoneal Adhesions ◽  
Prosthetic Materials

Peritoneal adhesions to prosthetic materials

2000 ◽  
Vol 14 (10) ◽  
pp. 960-963 ◽  
Author(s):  
W. W. Vrijland ◽  
F. Bonthuis ◽  
E. W. Steyerberg ◽  
R. L. Marquet ◽  
J. Jeekel ◽  
...  
Keyword(s):  
Peritoneal Adhesions ◽  
Prosthetic Materials

P–744 The features expression of some lymphocyte markers in the pelvic peritoneal adhesions’ tissue at reproductive age women

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Sulima ◽  
G Puchkina ◽  
A Davydova
Keyword(s):  
Immune Response ◽  
T Lymphocytes ◽  
Transforming Growth Factor ◽  
Immunohistochemical Study ◽  
Abdominal Cavity ◽  
Adhesion Formation ◽  
Reproductive Age ◽  
Immunohistochemical Method ◽  
Women Of Reproductive Age ◽  
Peritoneal Adhesions

Abstract Study question To study the expression of CD4, CD8, CD20, CD 138 in the tissue of the pelvic peritoneal adhesions at women of reproductive age. Summary answer Immunohistochemical study of pelvic adhesions revealed the CD8-positive cells is directly involved in the formation of the immune response at the late stages of adhesiogenesis. What is known already One of the reason identifies the high frequency of adhesion formation is the presence of inflammation in the abdominal cavity with different severity and origin. It is known that Insufficiency of the fibrinolytic system, increased levels of a number of cytokines, including transforming growth factor- β1, and tissue hypoxia induce neoangiogenesis and fibrotization of the fibrin matrix, which leads to the formation of adhesions. Data on expression of CD4, CD8, CD20, CD138/syndecan–1 in the pelvic peritoneal adhesions in connection with their prescription, localization and origin is absent at accessible literature. Study design, size, duration Two hundred infertile women (aged 19–49 yrs) with pelvic peritoneal adhesions, who were underwent operative laparoscopy and adhesiolysis. Participants/materials, setting, methods The material for this study was the fragments of surgical material (adhesions and their parts) n = 200, taken from the women of reproductive age who suffered with infertility during operative laparoscopy.The morphological and immunohistochemical study of adhesions were carried out by standard techniques using paraffin blocks, reagents of Dako and monoclonal antibodies to CD4 (Clone 4B12 Ready-to-Use),CD8 (Clone C8/144B Ready-to-Use), CD20 (Clone L26 Ready-to-Use),CD138/syndecan–1 (Clone MI15 Ready-to-Use) of Abcam with automatic coloring Dako Cytomation. Main results and the role of chance To assess the population composition of these cell infiltrates, as well as individual diffusely located inflammatory cells, an immunohistochemical method with the main lymphocytic markers (CD4, CD8, CD20, CD138) was used. First of all, it is necessary to note the complete absence of CD20-positively colored cells in all observations, which indicates that at the final stage of the formation of adhesions, there is no element of the B-lymphocytic immune response. In an immunohistochemical study with syndecan–1 (CD138) antibodies, we identified a small number of positively colored cells that were located mainly perivascular, as part of mononuclear infiltrates. Quantitative analysis showed that the number of such cells is 0.8±0.2. When studying CD4–positive T-lymphocytes, it was found that they are usually located in the form of band-shaped infiltrates and focal perivascular clusters. The number of CD4-positive cells in the spike tissue is 5.6±0.2. CD8-positive cells were located mainly submesothelial, and in the form of perivascular clusters, the number of such cells was 9.2±0.6. Limitations, reasons for caution Age limitation, only women aged 19–49 yrs took part in this study. Exclusion criteria were the following for the groups: acute gynecological diseases, malignant diseases of female genitalia and ovarian tumors. Wider implications of the findings: The absence of B-cells in the “mature” adhesions’ tissue was found.The number of CD8-positive cells in our study was 1.5 times higher than the number of CD4-positive T-lymphocytes.CD4-positive T-lymphocytes play an important role and their number significantly prevails over the number of CD8-positive T-lymphocytes at the initial stages of adhesiogenesis. Trial registration number Case control study

scholarly journals Prevention of peritoneal adhesions using antioxidant complex in the experiment

2011 ◽  
Vol 10 (6) ◽  
pp. 45-51
Author(s):  
V. I. Tikhonov ◽  
M. B. Plotnikov ◽  
S. V. Logvinov ◽  
M. Yu. Grishchenko ◽  
D. A. Shkatov ◽  
...  
Keyword(s):  
Complex Formation ◽  
Abdominal Cavity ◽  
Peritoneal Adhesions

In the analysis of known ways to prevent adhesions, by which the proposed alternative effects on the formation of adhesions in the abdominal cavity. We analyze the effect of antioxidant complex formation of adhesions in the experiment.

scholarly journals Effect of Bletilla striata on the Prevention of Postoperative Peritoneal Adhesions in Abrasion-Induced Rat Model

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
BoTong Liu ◽  
QiuHua Zhang ◽  
Xiao Wu ◽  
YanJun Fu ◽  
Hui Wang ◽  
...  
Keyword(s):  
Western Blot ◽  
Transforming Growth Factor Beta ◽  
Immunohistochemical Staining ◽  
Transforming Growth Factor ◽  
Smooth Muscle Actin ◽  
Abdominal Cavity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Peritoneal Adhesions ◽  
Bletilla Striata ◽  
Masson Trichrome Staining

Postoperative peritoneal adhesions (PPAs) constitute a common complication of abdominal surgery with a high incidence. Bletilla striata (BS) is an important hemostatic drug used in China for nearly 2000 years. The purpose of this study was to investigate the effect of Bletilla striata on postoperative intestinal adhesion in rats. PPA was induced by cecal wall abrasion, and Bletilla striata was injected to observe its effect on adhesion in rats. The adhesion and inflammation score were assessed through visual observation and histopathologic evaluation. The levels of interleukin-1 (IL-1β), tumor necrosis factor (TNF-α), and interleukin-17F (IL-17F) in abdominal cavity and interleukin-6 (IL-6) in plasma were measured by enzyme-linked immunosorbent assay (ELISA) at 6 hours, 12 hours, 24 hours, and 1 week after operation. The tissue level of transforming growth factor beta-1 (TGF-β1) was also determined by ELISA on the seventh day after surgery. The expressions of collagen and TNF-α were, respectively, detected by Masson trichrome staining and immunohistochemical staining. The expression of TGF-β1 and alpha smooth muscle actin (α-SMA) was detected by Western blot. The result showed that Bletilla striata has obvious preventive effect on PPAs and celiac inflammation of PPAs. Bletilla striata could significantly reduce the level of IL-17F abdominal cavity and IL-6 in plasma. Masson trichrome staining and immunohistochemical staining results showed that Bletilla striata also decreased the expression of TNF-α and collagen. Western blot results showed that Bletilla striata decreased the expression of α-SMA and TGF-β1. Our results suggest that Bletilla striata decreased the development of abdominal adhesion in abrasion-induced model of rats and reduced the expression of the important substance which increased in PPAs. Bletilla striata can be further studied as a new and cheaper antiadhesive substance.

scholarly journals Comparative study of peritoneal adhesions after intraperitoneal implantation in rats of meshes of polypropylene versus polypropylene/polyglecaprone versus polyester/porcine collagen

2019 ◽  
Vol 34 (6) ◽  
Author(s):  
Waston Gonçalves Ribeiro ◽  
Diego Vinnicyus Santos Rodrigues ◽  
Francisco Felipe Moreira Atta ◽  
Izabelle Smith Frazão Ramos ◽  
Fabiola Nassar Sousa Frazão ◽  
...  
Keyword(s):  
Comparative Study ◽  
Peritoneal Adhesions ◽  
Porcine Collagen

scholarly journals Complex Laparoscopic Myomectomy with Severe Adhesions Performed with Proper Preventive Measures and Power Morcellation Provides a Safe Choice in Certain Infertility Cases

2016 ◽  
Vol 2016 ◽  
pp. 1-4
Author(s):  
Jaime Alfaro-Alfaro ◽  
María de los Ángeles Flores-Manzur ◽  
Roberto Nevarez-Bernal ◽  
Rodrigo Ayala-Yáñez
Keyword(s):  
Transvaginal Ultrasound ◽  
Abdominal Cavity ◽  
Adhesion Formation ◽  
Uterine Fibroids ◽  
Malignant Cell ◽  
The United States ◽  
Laparoscopic Myomectomy ◽  
Peritoneal Adhesions ◽  
Bicornuate Uterus ◽  
Power Morcellation

Laparoscopic myomectomy offers a real benefit to infertile patients with uterine fibroids and peritoneal adhesions. The procedure requires a skilled surgeon and laparoscopy technique to minimize adhesion formation and other proven benefits. Restrictions arise since this procedure requires power morcellation for fibroid tissue extraction. Two years ago, the Food and Drug Administration in the United States of America (FDA) issued the alert on power morcellation for uterine leiomyomas, addressing the risk of malignant cell spreading within the abdominal cavity (actual risk assessment from 1 in 360 to 1 in 7400 cases). We review a 30-year-old female, without previous gestations, hypermenorrhea, intermenstrual bleeding, and chronic pelvic pain. Transvaginal ultrasound reports multiple fibroids in the right portion of a bicornuate uterus. Relevant history includes open myomectomy 6 years before and a complicated appendectomy, developing peritonitis within a year. Laparoscopy revealed multiple adhesions blocking uterine access, a bicornuate uterus, and myomas in the expected site. Myomectomy was performed utilizing power morcellation with good results. FDA recommendations have diminished this procedure’s selection, converting many to open variants. This particular case was technically challenging, requiring morcellation, and safety device deployment was impossible, yet the infertility issue was properly addressed. Patient evaluation, safety measures, and laparoscopy benefits may outweigh the risks in particular cases as this one.

scholarly journals Endogenously-Produced Hyaluronan and Its Potential to Regulate the Development of Peritoneal Adhesions

Biomolecules ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 45
Author(s):  
Anna Kocurkova ◽  
Kristina Nesporova ◽  
Miriam Sandanusova ◽  
Michaela Kerberova ◽  
Katerina Lehka ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Abdominal Surgery ◽  
Glucuronic Acid ◽  
Abdominal Cavity ◽  
Peritoneal Membrane ◽  
Peritoneal Adhesions ◽  
Mesenchymal Transition ◽  
Sulfated Glycosaminoglycan ◽  
Fibrotic Tissue

Formation of peritoneal adhesions (PA) is one of the major complications following intra-abdominal surgery. It is primarily caused by activation of the mesothelial layer and underlying tissues in the peritoneal membrane resulting in the transition of mesothelial cells (MCs) and fibroblasts to a pro-fibrotic phenotype. Pro-fibrotic transition of MCs—mesothelial-to-mesenchymal transition (MMT), and fibroblasts activation to myofibroblasts are interconnected to changes in cellular metabolism and culminate in the deposition of extracellular matrix (ECM) in the form of fibrotic tissue between injured sides in the abdominal cavity. However, ECM is not only a mechanical scaffold of the newly synthetized tissue but reciprocally affects fibrosis development. Hyaluronan (HA), an important component of ECM, is a non-sulfated glycosaminoglycan consisting of N-acetyl-D-glucosamine (GlcNAc) and D-glucuronic acid (GlcUA) that can affect the majority of processes involved in PA formation. This review considers the role of endogenously produced HA in the context of different fibrosis-related pathologies and its overlap in the development of PA.

scholarly journals Comparative Study on the Color Stability of Temporary Prosthetic Materials for Dental CAD-CAM

2016 ◽  
Vol 38 (3) ◽  
pp. 165-173
Author(s):  
Hye-Eun Lee ◽  
Won-Soo Kim ◽  
Jung-suk Kim ◽  
Ju-hee Lee ◽  
Seuck-Jae Won
Keyword(s):  
Comparative Study ◽  
Color Stability ◽  
Prosthetic Materials ◽  
Cad Cam
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