scholarly journals Effects of Nano-selenium on Blood Biochemistry, Liver Antioxidant Activity and GPx-1 mRNA Expression in Rabbits

2016 ◽  
Author(s):  
Shunyi Qin ◽  
Fu Chen ◽  
Fanghong Zhao ◽  
Tianming Jin ◽  
Jifei Ma
Keyword(s):  
Antioxidant Activity ◽  
Mrna Expression ◽  
Blood Biochemistry

scholarly journals Nrf2–ARE Signaling Acts as Master Pathway for the Cellular Antioxidant Activity of Fisetin

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 708 ◽  
Author(s):  
Huihui Zhang ◽  
Wan Zheng ◽  
Xiangling Feng ◽  
Fei Yang ◽  
Hong Qin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mrna Expression ◽  
Protein Level ◽  
Nuclear Accumulation ◽  
Luciferase Reporter ◽  
Heme Oxygenase 1 ◽  
Quinone Oxidoreductase ◽  
Glutamate Cysteine Ligase ◽  
Cellular Antioxidant Activity ◽  
Nrf2 Signaling Pathway

Fisetin, a dietary flavonoid, is reported to have cellular antioxidant activity with an unclear mechanism. In this study, we investigated the effect of fisetin on the nuclear factor, erythroid 2-like 2 (Nrf2) signaling pathway in HepG2 cells to explore the cellular antioxidant mechanism. Fisetin upregulated the mRNA expression of heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and NAD(P)H quinone oxidoreductase-1 (NQO1), and induced the protein of HO-1 but had no significant effect on the protein of GCLC, GCLM and NQO1. Moreover, nuclear accumulation of Nrf2 was clearly observed by immunofluorescence analysis and western blotting after fisetin treatment, and an enhanced luciferase activity of antioxidant response element (ARE)-regulated transactivation was obtained by dual-luciferase reporter gene assays. In addition, fisetin upregulated the protein level of Nrf2 and downregulated the protein level of Kelch-like ECH-associated protein 1 (Keap1). However, fisetin had no significant effect on Nrf2 mRNA expression. When protein synthesis was inhibited with cycloheximide (CHX), fisetin prolonged the half-life of Nrf2 from 15 min to 45 min. When blocking Nrf2 degradation with proteasome inhibitor MG132, ubiquitinated proteins were enhanced, and fisetin reduced ubiquitination of Nrf2. Taken together, fisetin translocated Nrf2 into the nucleus and upregulated the expression of downstream HO-1 gene by inhibiting the degradation of Nrf2 at the post-transcriptional level. These data provide the molecular mechanism to understand the cellular antioxidant activity of fisetin.

scholarly journals Effect of Soybean Isoflavones on Growth Performance, Immune Function, and Viral Protein 5 mRNA Expression in Broiler Chickens Challenged with Infectious Bursal Disease Virus

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 247
Author(s):  
Mahmoud Mostafa Azzam ◽  
Shou-qun JIANG ◽  
Jia-li CHEN ◽  
Xia-jing LIN ◽  
Zhong-yong GOU ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mrna Expression ◽  
Disease Virus ◽  
Viral Protein ◽  
Infectious Bursal Disease Virus ◽  
Broiler Chickens ◽  
Infected Group ◽  
Infectious Bursal Disease ◽  
Infected Control ◽  
Bursal Disease

A total of 200 one-day-old male broilers were assigned to five groups, and each group consisted of four replicates with 10 birds per replicate. Chicks were fed the basal diet with 0 (non-infected control), 0 (infected control), 10, 20, and 40 mg/kg soybean isoflavones (SI) for 42 days. At 21 days of age, chickens were inoculated with an infectious bursal dose (causing 50% morbidity) of the infectious bursal disease virus (IBDV) BC 6/85 strain by the eye-drop and nasal route (except for the non-infected group). Average daily gain (ADG) and average daily feed intake (ADFI) decreased (p < 0.05) in broilers infected with infectious bursal disease virus (IBDV) from 22 to 42 days. However, infected broilers fed 10 and 20 mg SI/kg had the maximum (p <0.05) ADG and ADFI from 1 to 42 days. Body weight (BW) increased (p < 0.05) in infected broilers fed the 10 and 20 mg SI /kg diet. The bursa weight at 7 days post-infection (dpi) was increased (p < 0.05) by the supplemental 10 mg SI/kg diet. Infected broilers showed the highest (p < 0.05) bursa lesions, with an average score of 4.0 ± 0.0, while the severity of bursa lesions was decreased (p < 0.05) at 3 dpi and 7 dpi by the supplemental 20 mg SI/kg diet. Supplemental SI at 20 mg/kg decreased (p < 0.05) the viral protein 5 (VP5) mRNA expression at 3 dpi and 7 dpi. The level of interferon gamma (IFNγ) was elevated (p < 0.05) in the infected group at 3 dpi and 7 dpi as compared with the control group, while its level was decreased (p < 0.05) by supplemental 10 mg/kg SI at 3 dpi. The level of nuclear factor κB in the bursal tissue showed the lowest value (p < 0.05) with supplemental 10 and 20 mg SI/kg diet at 7 dpi. Supplemental 10, 20, 40 mg/kg SI improved (p < 0.05) the serum total antioxidant activity (T-AOC) in infected broilers at 3 dpi. In addition, the serum level of malondialdehyde (MDA) decreased (p < 0.05) in the group fed 20 mg/kg SI at 7 dpi. In conclusion, supplemental 10~20 mg/kg SI may have a positive effect on broiler chickens infected with IBDV, probably because SI decrease the severity of bursa lesions and viral protein 5 mRNA expression, and have strong antioxidant activity.

Mangosteen peel extract exhibits cellular antioxidant activity by induction of catalase and heme oxygenase-1 mRNA expression

2018 ◽  
Vol 42 (3) ◽  
pp. e12511 ◽  
Author(s):  
Nattapon Jaisupa ◽  
Primchanien Moongkarndi ◽  
Pattamapan Lomarat ◽  
Jutima Samer ◽  
Vatchara Tunrungtavee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mrna Expression ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Cellular Antioxidant Activity ◽  
Mangosteen Peel ◽  
Peel Extract
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