Mangosteen peel extract exhibits cellular antioxidant activity by induction of catalase and heme oxygenase-1 mRNA expression

2018 ◽  
Vol 42 (3) ◽  
pp. e12511 ◽  
Author(s):  
Nattapon Jaisupa ◽  
Primchanien Moongkarndi ◽  
Pattamapan Lomarat ◽  
Jutima Samer ◽  
Vatchara Tunrungtavee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mrna Expression ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Cellular Antioxidant Activity ◽  
Mangosteen Peel ◽  
Peel Extract

scholarly journals Nrf2–ARE Signaling Acts as Master Pathway for the Cellular Antioxidant Activity of Fisetin

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 708 ◽  
Author(s):  
Huihui Zhang ◽  
Wan Zheng ◽  
Xiangling Feng ◽  
Fei Yang ◽  
Hong Qin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mrna Expression ◽  
Protein Level ◽  
Nuclear Accumulation ◽  
Luciferase Reporter ◽  
Heme Oxygenase 1 ◽  
Quinone Oxidoreductase ◽  
Glutamate Cysteine Ligase ◽  
Cellular Antioxidant Activity ◽  
Nrf2 Signaling Pathway

Fisetin, a dietary flavonoid, is reported to have cellular antioxidant activity with an unclear mechanism. In this study, we investigated the effect of fisetin on the nuclear factor, erythroid 2-like 2 (Nrf2) signaling pathway in HepG2 cells to explore the cellular antioxidant mechanism. Fisetin upregulated the mRNA expression of heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), and NAD(P)H quinone oxidoreductase-1 (NQO1), and induced the protein of HO-1 but had no significant effect on the protein of GCLC, GCLM and NQO1. Moreover, nuclear accumulation of Nrf2 was clearly observed by immunofluorescence analysis and western blotting after fisetin treatment, and an enhanced luciferase activity of antioxidant response element (ARE)-regulated transactivation was obtained by dual-luciferase reporter gene assays. In addition, fisetin upregulated the protein level of Nrf2 and downregulated the protein level of Kelch-like ECH-associated protein 1 (Keap1). However, fisetin had no significant effect on Nrf2 mRNA expression. When protein synthesis was inhibited with cycloheximide (CHX), fisetin prolonged the half-life of Nrf2 from 15 min to 45 min. When blocking Nrf2 degradation with proteasome inhibitor MG132, ubiquitinated proteins were enhanced, and fisetin reduced ubiquitination of Nrf2. Taken together, fisetin translocated Nrf2 into the nucleus and upregulated the expression of downstream HO-1 gene by inhibiting the degradation of Nrf2 at the post-transcriptional level. These data provide the molecular mechanism to understand the cellular antioxidant activity of fisetin.

scholarly journals FORMULASI NANOEMULGEL EKSTRAK KULIT MANGGIS (Garcinia Mangostana L.)

2019 ◽  
Vol 1 (3) ◽  
pp. 166-176
Author(s):  
Hilda Damayanti ◽  
Saleh Wikarsa ◽  
Garnadi Jafar
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Garcinia Mangostana ◽  
Mangosteen Peel ◽  
Dpph Method ◽  
Nanoemulsion Gel ◽  
Materials Used ◽  
Peel Extract

Antioxidant-containing cosmetic has antiaging therapy that can inhibit the free radical formation. Mangosteen peel extract has very strong antioxidant activity. To enhance the effect and comfortness of mangosteen peel extract use on the skin, it could be made into nanoemulgel. The article provides the information about method of preparation and evaluation of nanoemulsion-gel. The purpose of this study was to formulate a stable microemulgel of mangosteen peel extractusing halal materials declared halal according to Islamic Shari’a.  the materials used don’t contain carrion, blood, pig and/ animals that don’t conform to Islamic Shari’a. Microemulgel mangosteen peel extract was made by varying plantacare® 1200 UP concentration as cosurfactant (5, 10, 15, 20 and 25%) in the microemulsion and it was incorporated into the gel base. Evaluations were included the antioxidant activity test and organoleptic, pH, viscosity, stability, particle size analysis and panelist test. The antioxidant activity determined by DPPH method showed that IC50 value of mangosteen peel extract was 5.54 ppm. The third microemulsion formula containing cosurfactant of 15% resulted in the best results in that the parameter of the product can be penetrated by ray laser was at particle size of  23.65 nm, was determined by tranmission Electron Microscopy (TEM). Microemulgel containing Viscolam® MAC 10 of seven percent gave the stable formula proofed by freeze thaw and sentrifuga test. The five microemulgel formulations were stable.    

Heme Oxygenase-1 mRNA Expression in Egyptian Patients With Chronic Liver Disease

2012 ◽  
Vol 12 (4) ◽  
pp. 278-285 ◽  
Author(s):  
Sahar Saad El-Din Bessa ◽  
Ehab Mostafa Mohamed Ali ◽  
Abeer El-Sayed Abd El-Wahab ◽  
Sherif Abd El-Monem Nor El-Din
Keyword(s):  
Liver Disease ◽  
Mrna Expression ◽  
Chronic Liver Disease ◽  
Heme Oxygenase ◽  
Chronic Liver ◽  
Heme Oxygenase 1 ◽  
Egyptian Patients

Heme Oxygenase-1 mRNA Expression in Chronic Hepatitis B

2005 ◽  
Vol 100 ◽  
pp. S130
Author(s):  
J. Y. Lee ◽  
M. K. Jang ◽  
J. H. Lee ◽  
H. Y. Kim ◽  
J. Y. Yoo
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Mrna Expression ◽  
Chronic Hepatitis B ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1

Heme Oxygenase-1 mRNA Expression in the Lung during Murine Traumatic Shock: Effect of rsPSGL.1g

2002 ◽  
Vol 96 (Sup 2) ◽  
pp. A420
Author(s):  
Alexander G. Minchenko ◽  
Irina L. Opentanova ◽  
Valerie E. Armstead
Keyword(s):  
Mrna Expression ◽  
Heme Oxygenase ◽  
Traumatic Shock ◽  
Heme Oxygenase 1 ◽  
Shock Effect

CGS 26303 Upregulates mRNA Expression of Heme Oxygenase-1 in Brain Tissue of Rats Subjected to Experimental Subarachnoid Hemorrhage

2004 ◽  
Vol 44 (Supplement 1) ◽  
pp. S474-S478 ◽  
Author(s):  
Chun-Po Yen ◽  
Shih-Chieh Chen ◽  
Tze-Kan Lin ◽  
Shu-Chuan Wu ◽  
Chao-Yuah Chang ◽  
...  
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Mrna Expression ◽  
Brain Tissue ◽  
Heme Oxygenase ◽  
Heme Oxygenase 1 ◽  
Experimental Subarachnoid Hemorrhage

scholarly journals Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Mohamed Elfeky ◽  
Ryuji Kaede ◽  
Yuko Okamatsu-Ogura ◽  
Kazuhiro Kimura
Keyword(s):  
Mrna Expression ◽  
Heme Oxygenase ◽  
High Mobility ◽  
Heme Oxygenase 1 ◽  
Amp Kinase ◽  
High Mobility Group Protein ◽  
Macrophage Cells ◽  
Compound C ◽  
Tlr4 Mrna ◽  
Anti Inflammatory

High mobility group protein B1 (HMGB1) is a late inflammatory mediator that exaggerates septic symptoms. Adiponectin, an adipokine, has potent anti-inflammatory properties. However, possible effects of adiponectin on lipopolysaccharide- (LPS-) induced HMGB1 release are unknown. The aim of this study was to investigate effects of full length adiponectin on HMGB1 release in LPS-stimulated RAW 264 macrophage cells. Treatment of the cells with LPS alone significantly induced HMGB1 release associated with HMGB1 translocation from the nucleus to the cytosol. However, prior treatment with adiponectin suppressed LPS-induced HMGB1 release and translocation. The anti-inflammatory cytokine interleukin- (IL-) 10 similarly suppressed LPS-induced HMGB1 release. Adiponectin treatment decreased toll-like receptor 4 (TLR4) mRNA expression and increased heme oxygenase- (HO-) 1 mRNA expression without inducing IL-10 mRNA, while IL-10 treatment decreased TLR2 and HMGB1 mRNA expression and increased the expression of IL-10 and HO-1 mRNA. Treatment with the HO-1 inhibitor ZnPP completely prevented the suppression of HMGB1 release by adiponectin but only partially inhibited that induced by IL-10. Treatment with compound C, an AMP kinase (AMPK) inhibitor, abolished the increase in HO-1 expression and the suppression of HMGB1 release mediated by adiponectin. In conclusion, our results indicate that adiponectin suppresses HMGB1 release by LPS through an AMPK-mediated and HO-1-dependent IL-10-independent pathway.

scholarly journals Plant extract activities as antioxidant and antibiofilm against chicken gut bacteria

2018 ◽  
Vol 23 (1) ◽  
pp. 11
Author(s):  
Erika Gracia ◽  
S. Magdalena ◽  
Elizabeth Wina ◽  
Arnold P. Sinurat ◽  
Tresnawati Purwadaria
Keyword(s):  
Antioxidant Activity ◽  
Pathogenic Bacteria ◽  
Secondary Compounds ◽  
Gut Bacteria ◽  
Antibiofilm Activity ◽  
Growth Promoter ◽  
Plant Secondary Compounds ◽  
Biofilm Inhibition ◽  
Mangosteen Peel ◽  
Peel Extract

The occurrence of microbial resistance against antibiotic due to the subtherapeutic dosage of antibiotic growth promoter (AGP) in poultry can be prevented by the antibiofilm substance. Plant secondary compounds have some activities like antioxidant, antimicrobial, and antibiofilm. This research was conducted to obtain the plant with the highest activity of antibiofilm and also antioxidant by analyzing several plant secondary compounds as antioxidant and antibiofilm against chicken’s gut bacteria. The tested plants were clove leaves, leaffruit plants, mangosteen peel, cashew nut shell, guava leaves, and bay leaves. These plants were extracted with methanol or n-hexane using sonication method. The antioxidant activity as the IC50 value of the plant methanol extracts were determined using α,α-diphenyl-β-picrylhydrazyl (DPPH) assay. The biofilm inhibition activity was tested against Escherichia coli, Salmonella enteritidis, and Staphylococcus aureus ATCC® 29213TM using methanol and n-hexane extracts. All of the samples had antioxidant activity. The clove leaves and leaffruit plants had the highest antioxidant activity, while mangosteen peel extract in methanol had the highest antibiofilm activity against all tested bacteria. The species of bacteria also affected the antibiofilm activity. E. coli and S. enteritidis were more resistant to antibiofilm then S. aureus. Mangosteen peel extract which showed high antioxidant and antibiofilm activity is potential to be used as a feed additive to control the pathogenic bacteria.

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