scholarly journals Gynandromorphic Specimens of Culex erythrothorax, Culex pipiens Complex, Culex tarsalis, and Culiseta incidens Collected in Northern San Joaquin Valley, California

2020 ◽  
Vol 36 (3) ◽  
pp. 208-211
Author(s):  
Sumiko R. De La Vega ◽  
David J. Smith ◽  
John A. Fritz ◽  
Eddie F. Lucchesi ◽  
Shaoming Huang

ABSTRACT Gynandromorphic insects bear both male and female characteristics. In mosquitoes, gynandromorphy has been documented in multiple species and it can include many different combinations of sex-specific traits such as plumose or filiform antennae, short or elongated palpi, and male or female terminalia. Here we report 4 gynandromorphic specimens of Culex erythrothorax, Cx. pipiens complex, Cx. tarsalis, and Culiseta incidens collected in the Northern San Joaquin Valley of California. Each of the specimens exhibited heads with female characteristics of short palpi and filiform antennae while displaying fully developed external male terminalia including basistyles and dististyles. The potential implication of gynandromorphism in genetic control of mosquito vectors is discussed.

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 686
Author(s):  
Jakob Thannesberger ◽  
Nicolas Rascovan ◽  
Anna Eisenmann ◽  
Ingeborg Klymiuk ◽  
Carina Zittra ◽  
...  

Mosquitoes are the most important vectors for arthropod-borne viral diseases. Mixed viral infections of mosquitoes allow genetic recombination or reassortment of diverse viruses, turning mosquitoes into potential virologic mixing bowls. In this study, we field-collected mosquitoes of different species (Aedes aegypti and Culex pipiens complex), from different geographic locations and environments (central Europe and the Caribbean) for highly sensitive next-generation sequencing-based virome characterization. We found a rich virus community associated with a great diversity of host species. Among those, we detected a large diversity of novel virus sequences that we could predominately assign to circular Rep-encoding single-stranded (CRESS) DNA viruses, including the full-length genome of a yet undescribed Gemykrogvirus species. Moreover, we report for the first time the detection of a potentially zoonotic CRESS-DNA virus (Cyclovirus VN) in mosquito vectors. This study expands the knowledge on virus diversity in medically important mosquito vectors, especially for CRESS-DNA viruses that have previously been shown to easily recombine and jump the species barrier.


2021 ◽  
Vol 6 (2) ◽  
pp. 45
Author(s):  
Ana Klobucar ◽  
Vladimir Savic ◽  
Marcela Curman Posavec ◽  
Suncica Petrinic ◽  
Urska Kuhar ◽  
...  

In the period from 2015 to 2020, an entomological survey for the presence of West Nile virus (WNV) and Usutu virus (USUV) in mosquitoes was performed in northwestern Croatia. A total of 20,363 mosquitoes were sampled in the City of Zagreb and Međimurje county, grouped in 899 pools and tested by real-time RT-PCR for WNV and USUV RNA. All pools were negative for WNV while one pool each from 2016 (Aedes albopictus), 2017 (Culex pipiens complex), 2018 (Cx. pipiens complex), and 2019 (Cx. pipiens complex), respectively, was positive for USUV. The 2018 and 2019 positive pools shared 99.31% nucleotide homology within the USUV NS5 gene and both clustered within USUV Europe 2 lineage. The next-generation sequencing of one mosquito pool (Cx. pipiens complex) collected in 2018 in Zagreb confirmed the presence of USUV and revealed several dsDNA and ssRNA viruses of insect, bacterial and mammalian origin.


2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Meriem Bkhache ◽  
Fatim-Zohra Tmimi ◽  
Omar Charafeddine ◽  
Chafika Faraj ◽  
Anna-Bella Failloux ◽  
...  

2020 ◽  
Vol 168 (2) ◽  
pp. 148-157
Author(s):  
Mihaela Kavran ◽  
Igor Pajović ◽  
Dušan Petrić ◽  
Aleksandra Ignjatović‐Ćupina ◽  
Nedeljko Latinović ◽  
...  

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 1033 ◽  
Author(s):  
Pettersson ◽  
Shi ◽  
Eden ◽  
Holmes ◽  
Hesson

Mosquitoes harbor an extensive diversity of ‘insect-specific’ RNA viruses in addition to those important to human and animal health. However, because most studies of the mosquito virome have been conducted at lower latitudes, little is known about the diversity and evolutionary history of RNA viruses sampled from mosquitoes in northerly regions. Here, we compared the RNA virome of two common northern mosquito species, Culex pipiens and Culex torrentium, collected in south-central Sweden. Following bulk RNA-sequencing (meta-transcriptomics) of 12 libraries, comprising 120 specimens of Cx. pipiens and 150 specimens of Cx. torrentium, we identified 40 viruses (representing 14 virus families) of which 28 were novel based on phylogenetic analysis of the RNA-dependent RNA polymerase (RdRp) protein. Hence, we documented similar levels of virome diversity as in mosquitoes sampled from the more biodiverse lower latitudes. Many viruses were also related to those sampled on other continents, indicative of a widespread global movement and/or long host–virus co-evolution. Although the two mosquito species investigated have overlapping geographical distributions and share many viruses, several viruses were only found at a specific location at this scale of sampling, such that local habitat and geography may play an important role in shaping viral diversity in Culex mosquitoes.


2020 ◽  
Vol 164 ◽  
pp. 33-39
Author(s):  
Do Eun Lee ◽  
Heung-Chul Kim ◽  
Sung-Tae Chong ◽  
Terry A. Klein ◽  
Kwang Shik Choi ◽  
...  

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