Field Evaluation of Indoor Thermal Fog and Ultra-Low Volume Applications For Control of Aedes aegypti in Thailand

2017 ◽  
Vol 33 (2) ◽  
pp. 116-127 ◽  
Author(s):  
A. Ponlawat ◽  
J. F. Harwood ◽  
J. L. Putnam ◽  
C. Nitatsukprasert ◽  
A. Pongsiri ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Field Evaluation ◽  
Low Volume

scholarly journals SEMI-FIELD EVALUATION OF ULTRA-LOW VOLUME (ULV) GROUND SPRAY OF AQUALUER ® 20-20 AGAINST IRRADIATED AEDES AEGYPTI

2021 ◽  
Vol 68 (1) ◽  
pp. 63-69
Author(s):  
Vindhya S. Aryaprema
Keyword(s):  
Aedes Aegypti ◽  
Chemical Control ◽  
Control Method ◽  
Field Evaluation ◽  
Effective Control ◽  
Spray Application ◽  
Pyrethroid Insecticides ◽  
Host Seeking ◽  
Vector Management ◽  
Low Volume

Sterile insect technique (SIT) using irradiated mosquitoes is an effective control method capable of being assimilated into integrated vector management (IVM) programs. Chemical control of mosquitoes using ultra-low volume (ULV) spray applications of pyrethroid and organophosphate insecticides is already an essential component of IVM programs. Prior to their release in nature, irradiation of mosquitoes for SIT use can significantly impact the mosquito’s biology, specifically its host-seeking and feeding behavior. Little is known about how radiation exposure might impact a mosquito’s susceptibility to pyrethroid insecticides. The present study was carried out to evaluate the influence of Aqualuer ® 20-20 ULV applications on irradiated Aedes aegypti. Caged mosquito trials indicated that both male and female irradiated Ae. aegypti were as susceptible as their non-irradiated counterparts of the same population to Aqualuer 20-20 ULV application, with the highest mean percent mortalities achieved at the first 24h post-treatment period at both 30.5 m and 61 m downwind of the spray application path.

scholarly journals Single blinded semi-field evaluation of MAÏA® topical repellent ointment compared to unformulated 20% DEET against Anopheles gambiae, Anopheles arabiensis and Aedes aegypti in Tanzania

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Emmanuel Mbuba ◽  
Olukayode G. Odufuwa ◽  
Frank C. Tenywa ◽  
Rose Philipo ◽  
Mgeni M. Tambwe ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Anopheles Gambiae ◽  
Anopheles Arabiensis ◽  
Protective Efficacy ◽  
Latin Square ◽  
Field Evaluation ◽  
Sensu Stricto ◽  
Lower Limbs ◽  
Complete Protection ◽  
Regular User

Abstract Background N,N-Diethyl-3-methylbenzamide (DEET) topical mosquito repellents are effective personal protection tools. However, DEET-based repellents tend to have low consumer acceptability because they are cosmetically unappealing. More attractive formulations are needed to encourage regular user compliance. This study evaluated the protective efficacy and protection duration of a new topical repellent ointment containing 15% DEET, MAÏA® compared to 20% DEET in ethanol using malaria and dengue mosquito vectors in Bagamoyo Tanzania. Methods Fully balanced 3 × 3 Latin square design studies were conducted in large semi-field chambers using laboratory strains of Anopheles gambiae sensu stricto, Anopheles arabiensis and Aedes aegypti. Human volunteers applied either MAÏA® ointment, 20% DEET or ethanol to their lower limbs 6 h before the start of tests. Approximately 100 mosquitoes per strain per replicate were released inside each chamber, with 25 mosquitoes released at regular intervals during the collection period to maintain adequate biting pressure throughout the test. Volunteers recaptured mosquitoes landing on their lower limbs for 6 h over a period of 6 to 12-h post-application of repellents. Data analysis was conducted using mixed-effects logistic regression. Results The protective efficacy of MAÏA® and 20% DEET was not statistically different for each of the mosquito strains: 95.9% vs. 97.4% against An. gambiae (OR = 1.53 [95% CI 0.93–2.51] p = 0.091); 96.8% vs 97.2% against An. arabiensis (OR = 1.08 [95% CI 0.66–1.77] p = 0.757); 93.1% vs 94.6% against Ae. aegypti (OR = 0.76 [95% CI 0.20–2.80] p = 0.675). Average complete protection time (CPT) in minutes of MAÏA® and that of DEET was similar for each of the mosquito strains: 571.6 min (95% CI 558.3–584.8) vs 575.0 min (95% CI 562.1–587.9) against An. gambiae; 585.6 min (95% CI 571.4–599.8) vs 580.9 min (95% CI 571.1–590.7) against An. arabiensis; 444.1 min (95% CI 401.8–486.5) vs 436.9 min (95% CI 405.2–468.5) against Ae. aegypti. Conclusions MAÏA® repellent ointment provides complete protection for 9 h against both An. gambiae and An. arabiensis, and 7 h against Ae. aegypti similar to 20% DEET (in ethanol). MAÏA® repellent ointment can be recommended as a tool for prevention against outdoor biting mosquitoes in tropical locations where the majority of the people spend an ample time outdoor before going to bed.

Effect of Nozzle Orientation on Dispersion of Aqualuer 20-20 Sprayed by a Truck-Mounted Ultra-Low Volume Sprayer Against Caged Aedes aegypti

2017 ◽  
Vol 33 (3) ◽  
pp. 209-214 ◽  
Author(s):  
Muhammad Farooq ◽  
Ali Fulcher ◽  
Rui-De Xue ◽  
Michael L. Smith ◽  
Jeremy L. Anderson ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Low Volume ◽  
Nozzle Orientation

Field Evaluation of Coir Geotextile Reinforced Subgrade for Low Volume Pavements

2020 ◽  
pp. 1-13
Author(s):  
Anusudha Visvanathan ◽  
Sunitha Velayudhan ◽  
Samson Mathew
Keyword(s):  
Field Evaluation ◽  
Low Volume

scholarly journals Entomological Efficacy of Aerial Ultra-Low Volume Insecticide Applications Against Aedes aegypti (Diptera: Culicidae) in Mexico

2019 ◽  
Vol 56 (5) ◽  
pp. 1331-1337
Author(s):  
Fabián Correa-Morales ◽  
Felipe Dzul-Manzanilla ◽  
Wilbert Bibiano-Marín ◽  
José Vadillo-Sánchez ◽  
Anuar Medina-Barreiro ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Aedes Aegypti ◽  
Controlled Trial ◽  
Treatment Control ◽  
Randomized Controlled ◽  
Infestation Indices ◽  
Low Volume ◽  
Cluster Randomized ◽  
Weekly Cycles ◽  
Weekly Application

Abstract A cluster-randomized controlled trial quantified the entomological efficacy of aerial ultra-low volume (AULV) applications of the insecticide chlorpyrifos against Aedes aegypti in Puerto Vallarta, México, during November–October 2017. The trial involved 16 large (1 × 1 km) clusters distributed between treatment-control arms. Primary endpoint was the abundance of Ae. aegypti indoors (total adults, females, and blood-fed females) collected using Prokopack aspirators. After four consecutive weekly cycles of AULV, all adult Ae. aegypti infestation indices were significantly lower in the treatment arm (OR and IRR ≤ 0.28). Efficacy in reducing indoor Ae. aegypti increased with each weekly application cycle from 30 to 73% (total adults), 33 to 76% (females), and 45.5 to 89% (blood-fed females). Entomological indices remained significantly lower in the treatment arm up to 2 wk after the fourth spraying round. Performing AULV spraying can have significant and lasting entomological impact on Ae. aegypti as long as multiple (ideally four) spray cycles are implemented using an effective insecticide.

scholarly journals Dengue NS1 antigen kit shows high sensitivity for detection of recombinant dengue virus-2 NS1 antigen spiked with Aedes aegypti mosquitoes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Philip Raj Abraham ◽  
Bharathy R ◽  
Pradeep Kumar N ◽  
Ashwani Kumar
Keyword(s):  
Public Health ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Infected Patient ◽  
Specific Treatment ◽  
High Sensitivity ◽  
Field Evaluation ◽  
Denv Infection ◽  
Ns1 Protein ◽  
Ns1 Antigen

AbstractDengue, caused by the dengue virus (DENV) is a significant vector-borne disease. In absence of a specific treatment and vaccine, dengue is becoming a rising threat to public health. Currently, control of dengue mainly focuses on the surveillance of the mosquito vectors. Improved surveillance methods for DENV in mosquito populations would be highly beneficial to the public health. However, current methods of DENV detection in mosquitoes requires specialized equipment and expensive reagents and highly trained personnel. As an alternative, commercially available dengue NS1 antigen ELISA kits could be used for detection of DENV infection in Aedes aegypti mosquitoes. In this study, we explored the utility of commercially available Dengue NS1 antigen kit (J. Mitra & Co. Pvt. Ltd) for the detection of recombinant dengue virus-2 (rDENV-2) NS1 protein and serum of dengue infected patient spiked with Ae. aegypti mosquito pools. The kit was found to be highly sensitive and specific towards detection of all serotypes of DENV. Further, it could detect as low as 750 femto gram rDENV-2 NS1 protein. It was also observed that rDENV-2 NS1 antigen spiked with blood-fed and unfed mosquito pools could be detected. In addition, the kit also detected dengue infected patient serum spiked with Ae. aegypti mosquito pools. Overall, the Dengue NS1 antigen kit displayed high sensitivity towards detection of recombinant as well as serum NS1 protein spiked with Ae. aegypti mosquito pools and could be considered for the dengue virus surveillance after a field evaluation in Ae. aegypti mosquitoes.

scholarly journals Ultra-Low Volume Application of Spinosad (Natular 2EC) Larvicide as a Residual in a Tropical Environment Against Aedes and Anopheles Species

2018 ◽  
Vol 34 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Seth C. Britch ◽  
Kenneth J. Linthicum ◽  
Robert L. Aldridge ◽  
Frances V. Golden ◽  
Arissara Pongsiri ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Management Program ◽  
Dry Season ◽  
Tropical Environment ◽  
Integrated Vector Management ◽  
Anopheles Dirus ◽  
Important Species ◽  
Anopheles Species ◽  
Vector Management ◽  
Low Volume

ABSTRACT We investigated the efficacy of a liquid larvicide, Natular 2EC® (spinosad), applied with ultra-low volume sprayer as a residual application during the dry season in southeastern Thailand against 4 medically important species—Aedes aegypti, Ae. albopictus, Anopheles dirus, and An. minimus. We found that this larvicide could be applied as a residual to dry areas known to collect water and potentially still be effective after rains or irrigation, which could increase the flexibility and efficiency of an integrated vector management program targeting these species. This investigation also demonstrated, for the 1st time, efficacy of spinosad against An. minimus and An. dirus.

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