scholarly journals Ultra-Low Volume Application of Spinosad (Natular 2EC) Larvicide as a Residual in a Tropical Environment Against Aedes and Anopheles Species

2018 ◽  
Vol 34 (1) ◽  
pp. 58-62 ◽  
Author(s):  
Seth C. Britch ◽  
Kenneth J. Linthicum ◽  
Robert L. Aldridge ◽  
Frances V. Golden ◽  
Arissara Pongsiri ◽  
...  

ABSTRACT We investigated the efficacy of a liquid larvicide, Natular 2EC® (spinosad), applied with ultra-low volume sprayer as a residual application during the dry season in southeastern Thailand against 4 medically important species—Aedes aegypti, Ae. albopictus, Anopheles dirus, and An. minimus. We found that this larvicide could be applied as a residual to dry areas known to collect water and potentially still be effective after rains or irrigation, which could increase the flexibility and efficiency of an integrated vector management program targeting these species. This investigation also demonstrated, for the 1st time, efficacy of spinosad against An. minimus and An. dirus.

2018 ◽  
Vol 34 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Robert L. Aldridge ◽  
Frances V. Golden ◽  
Seth C. Britch ◽  
Jessika Blersch ◽  
Kenneth J. Linthicum

ABSTRACT Preemptive treatment of dry habitats with an ultra-low volume (ULV) residual larvicide may be effective in an integrated vector management program to control populations of container-inhabiting Aedes mosquitoes, key vectors of Zika, dengue, and chikungunya viruses. We exposed dry, artificial containers placed in exposed and protected locations to Natular 2EC (spinosad) larvicide applied with a truck-mounted ULV sprayer in a simulated urban setting in North Florida, and later introduced water and Ae. aegypti or Ae. albopictus larvae to conduct bioassays. Up to 50% mortality was observed in bioassays, indicating further analysis of spinosad as a residual treatment application.


2021 ◽  
Vol 37 (4) ◽  
pp. 242-249
Author(s):  
Eva A. Buckner ◽  
Katie F. Williams ◽  
Samantha Ramirez ◽  
Constance Darrisaw ◽  
Juliana M. Carrillo ◽  
...  

ABSTRACT Aedes aegypti is the predominant vector of dengue, chikungunya, and Zika viruses. This mosquito is difficult to control with conventional methods due to its container-inhabiting behavior and resistance to insecticides. Autodissemination of pyriproxyfen (PPF), a potent larvicide, has shown promise as an additional tool to control Aedes species in small-scale field trials. However, few large-scale field evaluations have been conducted. We undertook a 6-month-long large-scale field study to compare the effectiveness and operational feasibility of using In2Care Mosquito Traps (In2Care Traps, commercially available Aedes traps with PPF and Beauveria bassiana) compared to an integrated vector management (IVM) strategy consisting of source reduction, larviciding, and adulticiding for controlling Ae. aegypti eggs, larvae, and adults. We found that while the difference between treatments was only statistically significant for eggs and larvae (P < 0.05 for eggs and larvae and P > 0.05 for adults), the use of In2Care Traps alone resulted in 60%, 57%, and 57% fewer eggs, larvae, and adults, respectively, collected from that site compared to the IVM site. However, In2Care Trap deployment and maintenance were more time consuming and labor intensive than the IVM strategy. Thus, using In2Care Traps alone as a control method for large areas (e.g., >20 ha) may be less practical for control programs with the capacity to conduct ground and aerial larviciding and adulticiding. Based on our study results, we conclude that In2Care Traps are effective at suppressing Ae. aegypti and have the most potential for use in areas without sophisticated control programs and within IVM programs to target hotspots with high population levels and/or risk of Aedes-borne pathogen transmission.


2022 ◽  
Vol 4 (2) ◽  
Author(s):  
Katie Katie Williams ◽  
Samantha Ramirez ◽  
Christopher R. Lesser

AbstractAedes aegypti, the Dengue and Zika vector, is a domestic mosquito that is difficult to control. The challenge lies in the mosquito’s preference to lay its eggs in cryptic habitats such as fence post openings, buckets and bird baths, cups. Additionally, current methods of control are labor-intensive. We employed the WALS strategy to evaluate the operational efficacy of applying VectoBac WDG using a truck-mounted mister to control local populations of Ae. aegypti in urban settings with difficult-to-reach larval mosquito habitats. Our study was conducted in Cortez (Manatee County), Florida, USA (27° 27’ N, 82° 40’ W). We selected two study sites, one untreated control (21.9 ha) and one treatment site (23.1 ha) where historical data have shown high populations of Ae. aegypti based on weekly ovitrap surveillance. Weekly BGs and ovitraps (10–15 traps/site) were deployed to monitor adult population dynamics. A total of 50 bioassay jars were placed in the field each afternoon of the Bti application (8 application events) at fixed locations to collect droplets and gauge efficacy. We found significant reduction in female adults (P = 0.0002) and landing rate counts (P = 0.0058) as a result of treatment. Larval bioassays during the eight applications confirmed Bti deposit in a variety of coverage types regardless of placement in the yards. WALS applications with a truck-mounted mister can be effective at reducing disease carrying mosquito populations in residential areas and can be implemented in an integrated vector management program.


Author(s):  
Laura Harburguer ◽  
Paula V Gonzalez ◽  
Eduardo Zerba

Abstract Severe human arboviral diseases can be transmitted by the mosquito Aedes aegypti (Linnaeus), including dengue, chikungunya, Zika, and yellow fever. Adult control using spatial sprays with adulticides is recommended only when dengue outbreaks occur. In Argentina, mainly pyrethroids, like cis-permethrin, have been used as an adulticide, especially since 2008. The evolution and spread of resistance to insecticides is a major concern for vector control. This study reports for the first time pyrethroid resistance in Ae. aegypti adults from Argentina, in the city of Salvador Mazza (Salta). WHO discriminating doses of 0.75% were used for permethrin, 0.05% for deltamethrin, and 5% for malathion. Also the discriminating dose for cis-permethrin (0.6%) was calculated and evaluated for the first time. We found a resistance ratio 50 (RR50) of 10.3 (9.7–10.4) for cis-permethrin, which is considered as high resistance. Our results also indicated resistance to deltamethrin (22.6% mortality) and permethrin (53.6% mortality), and a total susceptibility to malathion (100% mortality). Results from this study highlight the importance of the correct use of insecticides within an Integrated Vector Management (IVM) approach and of early detection of resistance to enable Ae. aegypti control in Argentina. More studies are needed to determine the spread of mosquito resistance to pyrethroids.


Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 272
Author(s):  
Carlos Tur ◽  
David Almenar ◽  
Sandra Benlloch-Navarro ◽  
Rafael Argilés-Herrero ◽  
Mario Zacarés ◽  
...  

Aedes albopictus and Aedes aegypti are the main vectors of arboviral diseases such as dengue, Zika and chikungunya viruses. About a third of the world population is currently at risk of contracting Aedes-borne epidemics. In recent years, A. albopictus has drastically increased its distribution in many countries. In the absence of efficient mosquito vector control methods, the sterile insect technique (SIT) is presented as a very promising and environment-friendly control tool. The Agriculture Department of the Valencian Region is promoting an ongoing pilot project to evaluate the efficacy of an integrated vector management program (IVM) based on the use of the SIT as the main method of control. The laboratory studies for evaluating the entomological efficacy of SIT through the phased conditional testing process recommended by World Health Organization and the International Atomic Energy Agency (WHO-IAEA) are addressed. This study describes the routine operating procedures and quality control parameters for the medium-scale rearing of sterile male A. albopictus. More than 15 million sterile males have been produced and released in an area of 80 ha between 2018 and 2020. Of the initial L1 larvae, we recovered 17.2% of male pupae after sex sorting to be sterilized and released on the field, while the rest of the pupae remained available to maintain the rearing colony. The residual percentage of females after sex sorting was on average 0.17%. The obtained values in terms of production and quality control as well as the proposed rearing methodology can be useful for designing a medium-scale mosquito-rearing pipeline.


Author(s):  
Laith Yakob ◽  
Sebastian Funk ◽  
Anton Camacho ◽  
Oliver Brady ◽  
W. John Edmunds

2021 ◽  
Vol 68 (1) ◽  
pp. 63-69
Author(s):  
Vindhya S. Aryaprema

Sterile insect technique (SIT) using irradiated mosquitoes is an effective control method capable of being assimilated into integrated vector management (IVM) programs. Chemical control of mosquitoes using ultra-low volume (ULV) spray applications of pyrethroid and organophosphate insecticides is already an essential component of IVM programs. Prior to their release in nature, irradiation of mosquitoes for SIT use can significantly impact the mosquito’s biology, specifically its host-seeking and feeding behavior. Little is known about how radiation exposure might impact a mosquito’s susceptibility to pyrethroid insecticides. The present study was carried out to evaluate the influence of Aqualuer ® 20-20 ULV applications on irradiated Aedes aegypti. Caged mosquito trials indicated that both male and female irradiated Ae. aegypti were as susceptible as their non-irradiated counterparts of the same population to Aqualuer 20-20 ULV application, with the highest mean percent mortalities achieved at the first 24h post-treatment period at both 30.5 m and 61 m downwind of the spray application path.


ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 223-230
Author(s):  
S. Sunil Kumar ◽  
D.A. Evans ◽  
K. Muthulakshmi ◽  
T. DilipKumar ◽  
R. Heera Pillai ◽  
...  

Mosquito index study of three ecologically different ecozones of the Thiruvananthapuram district, Kerala showed sharp difference on the proportionate distribution of Aedes aegypti and Aedes albopictus. Human dengue viremia (HDV) was very high in those ecozones where A.aegypti density was high and HDV was low where A.albopictus was high. In a coastal zone of Thiruvananthapuram city, A. aegypti was the most abundant vector and in a hilly, arid suburban zone, A.albopictus was the abundant vector. In the urban zone both species of mosquitoes showed equal distribution. Study on the circulating serotypes in the serum of HDV by Single step single tube Multiplex PCR showed all the four serotypes viz DENV1, DENV2, DENV3 and DENV4 in patients of Thiruvananthapuram city, which indicated the possibility of Dengue Shock Syndrome, unless there is efficient vector management. Among the four dengue serotypes, Type 1 was the most abundant virus. Abundance of microhabitats in Thiruvananthapuram city, which support A. aegypti may be the reason for high prevalence of dengue fever in the urban zone.


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