scholarly journals DETECTION OF CAUSATIVE AGENTS OF TICK-BORNE INFECTIONS BY POLYMERASE CHAIN REACTION ASSAY CARRIED OUT AS A PART OF CLINICAL STUDIES IN THE “REPUBLICAN CLINICAL INFECTIOUS DISEASES HOSPITAL” OF ULAN-UDE

2018 ◽  
Vol 3 (4) ◽  
pp. 138-142
Author(s):  
E. V. Silchenko ◽  
L. M. Oshorova ◽  
I. Ts. Balzhinimaeva ◽  
E. I. Bondarenko ◽  
N. A. Dasheeva ◽  
...  
Keyword(s):  
Genetic Markers ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Genetic Material ◽  
Encephalitis Virus ◽  
Clinical Material ◽  
Unexpected Finding ◽  
Tick Borne Encephalitis ◽  
The Republic ◽  
Tick Borne Encephalitis Virus

The article is devoted to the study of vector-borne pathogens circulating in the Republic of Buryatia. The authors have carried out  identification of the genetic material of tick-borne encephalitis virus,  Borrellia burgdorferi, Borrellia miуamotoi, Coxiella burnetii and  Rickettsia sibirica in various samples of biological material. The  object of the study were whole blood, leukocyte fraction and blood  serum of patients admitted to the hospital in a state of fever that  occurred after sucking of the tick, as well as mites brought by the  population. A detailed analysis of the data showed that 11.7 % (9  out of 70) of the clinical material samples and 17.8 % (50 out of  281) of the tick samples had genetic markers of tick-borne  pathogens. In the material from the patients, the RNA of tick-borne  encephalitis virus was most often determined, and in the DNA of  Borrellia burgdorferi mites. In addition, the authors first confirmed  the etiologic role of Borrellia miуamotoi in the development of cases  of Lyme disease in the Republic. An unexpected finding was the  detection of Coxiella burnetii DNA in ticks and in clinical material. In  the Republic of Buryatia Q-fever was detected by laboratory  diagnostics for the first time. This fact confirms the circulation of this pathogen in the region. In this regard, the authors conducted a  retrospective analysis of the medical histories of patients who were found to have genetic markers of Q-fever.

scholarly journals Tick-Borne Encephalitis Virus, Coxiella burnetii & Brucella spp. in Milk, Kazakhstan

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
John Hay ◽  
Christina Farris ◽  
Phil Elzer ◽  
Alexei Andrushchenko ◽  
Sue Hagius ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Q Fever ◽  
Raw Milk ◽  
Encephalitis Virus ◽  
Western Kazakhstan ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
Species Specific ◽  
Positive Results ◽  
Positive Controls

Raw milk was collected from cows in western Kazakhstan in winter 2014-2015. Samples were defatted and frozen at -20C, then tested as follows. For tick-borne encephalitis virus, 65 samples were tested using the VectorBest TBEV antigen capture kit, with 9% positive. For Coxiella burnetii, 50 samples were assayed using a species-specific qPCR assay and all were negative, though positive controls were consistently positive. For Brucella spp., PCR, ELISA and FPA testing is ongoing, with some positive results. These data suggest that consumption of raw cow's milk in western Kazakhstan is a risk factor for tick-borne encephalitis and brucellosis. The risk for Q fever appears to be small during winter, but may be present at other times of the year.

TBE in Serbia

2021 ◽  
Author(s):  
Vladimir Petrović ◽  
Elizabeta Ristanović ◽  
Aleksandar Potkonjak
Keyword(s):  
Clinical Signs ◽  
Encephalitis Virus ◽  
Former Yugoslavia ◽  
Serological Testing ◽  
Tick Borne Encephalitis ◽  
The Republic ◽  
Tick Borne Encephalitis Virus

Tick-borne encephalitis virus (TBEV) was first isolated in the former Yugoslavia in 1953 from the blood of infected human patients in Slovenia.1 The virus was isolated from ticks in 1954, also in Slovenia.2 Thereafter a number of tick-borne encephalitis (TBE) foci were registered in the western part of the country, while in the Republic of Serbia such foci were not registered. In the period following 1969, no new infections with TBEV could be confirmed in the Republic of Serbia through the routine serological testing of samples from more than 1,000 patients with clinical signs of meningitis and encephalitis, as conducted in laboratories of the Institute of Immunobiology and Virology “Torlak” in Belgrade.3

scholarly journals Detection of Coxiella burnetii in ticks collected from cattle in several provinces of the Republic of Guinea

2019 ◽  
Vol 24 (5-6) ◽  
pp. 234-239
Author(s):  
Yulia A. Panferova ◽  
Olga A. Freylikhman ◽  
Nikolay K. Tokarevich ◽  
Ekaterina V. Naydenova ◽  
Kirill S. Zakharov ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Geographical Origin ◽  
Q Fever ◽  
Local Population ◽  
Genetic Material ◽  
Clinical Signs ◽  
Republic Of Guinea ◽  
Plasmid Analysis ◽  
The Republic ◽  
Almost All

Background. Q fever, or coxiellosis, is a natural focal disease characterized by polymorphism of clinical signs and can affect not only humans but also many species of animals. This infection is spread almost all over the world. On the African continent, the foci of coxiellosis infection endanger the local population and people arriving for temporary stay. Given that sick agricultural animals and their ectoparasites are markers of the presence of infection in the region, a study of the latter may be relevant to identify the potential foci of Q fever. This work aimed to identify Coxiella burnetii DNA from ixodic ticks collected from cattle in several provinces of Republic of Guinea and to type isolates using genetic markers (plasmid type) to enable their comparison with strains of different geographical origin. Methods. Using amplification technologies, we investigated the ticks obtained from cattle in the provinces of Boke and Kindia to detect Coxiella DNA. Results. The genetic material of the Q fever causative agent was detected in no more than 5% of the total number of samples studied. For positive samples, typing was performed using plasmid analysis. The isolates with the plasmid type QpH1 circulate in the Republic of Guinea. Conclusion. The findings were analyzed along with data from other researchers on the spread of Q fever in subequatorial Africa. The differences in the levels of prevalence of Coxiella in ticks in the territories of not only different countries but also within the same state can be determined by the prevalence among the hosts within herds. The risk of contamination with Q fever in endemic regions should be considered.

scholarly journals Search for Genetic Markers of Tick-Borne Encephalitis Virus for Its Specific Indication, Genomic Analysis

2020 ◽  
pp. 122-128
Author(s):  
N. I. Khammadov
Keyword(s):  
Nucleic Acids ◽  
Genetic Markers ◽  
Genomic Analysis ◽  
Positive Control ◽  
Nucleotide Sequences ◽  
Encephalitis Virus ◽  
Specific Marker ◽  
Nucleotide Variability ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

Objective of the study is to analyze the genetic markers of tick-borne encephalitis virus, which can be used to specifically indicate the maximum number of virus strains and isolates.Materials and methods. Plasmid DNA and nucleic acids of the tick, the genus Ixodes and Dermacentor, were used as amplified material. Polymerase chain reaction (PCR) was conducted on C1000 amplifier with a CFX96 optical unit (BioRad). The species (strain) variety of detected organisms, using the analyzed genetic markers, was determined in the nBLAST software utility. The design of the nucleotide sequences of primers and probes was performed using “Vector NTI 9.1.0” (Invitrogen Corporation). Nucleic acids were isolated by magnetic sorption with a reagent panel MAGNO-sorb. Primers and probes were synthesized at “Evrogen” company, Moscow, Russia. The reagents for PCR were manufactured by “Syntol” company, Moscow, Russia.Results and discussion. When indicating the genome of the tick-borne encephalitis virus, the main criterion for choosing a marker sequence is the specific detection of nucleic acids of only the desired microorganism. The genomewide nucleotide sequences of various strains and isolates of tick-borne encephalitis virus were analyzed to search for a specific marker nucleotide sequence. Mutual comparison of all the above mentioned genomes made it possible to determine 7 conventionally conservative loci characterized by minimal nucleotide variability. The further work was based on the results of alignment of the nucleotide sequences of tick-borne encephalitis virus isolates, including the nucleotide sequences of heterogeneous microorganisms; primers and probes were designed to amplify each of the marker loci; the most analytically significant oligonucleotides were developed based on loci 4 and 7. Amplification with oligonucleotide primers to indicate tick-borne encephalitis virus was effective, both in a separate PCR with a positive control, and in combination with tick DNA.

TBE in Serbia

2020 ◽  
Keyword(s):  
Clinical Signs ◽  
Encephalitis Virus ◽  
Former Yugoslavia ◽  
Serological Testing ◽  
Tick Borne Encephalitis ◽  
The Republic ◽  
Tick Borne Encephalitis Virus

Tick-borne encephalitis virus (TBEV) was first isolated in the former Yugoslavia in 1953 from the blood of infected human patients in Slovenia.1 The virus was isolated from ticks in 1954, also in Slovenia.2 Thereafter a number of tick-borne encephalitis (TBE) foci were registered in the western part of the country, while in the Republic of Serbia such foci were not registered. In the period following 1969, no new infections with TBEV could be confirmed in the Republic of Serbia through the routine serological testing of samples from more than 1,000 patients with clinical signs of meningitis and encephalitis, as conducted in laboratories of the Institute of Immunobiology and Virology “Torlak” in Belgrade.3

TBE in South Korea

2019 ◽  
Author(s):  
Joon Young Song
Keyword(s):  
South Korea ◽  
Human Case ◽  
Encephalitis Virus ◽  
Surveillance Studies ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

Although no human case of tick-borne encephalitis (TBE) has been documented in South Korea to date, surveillance studies have been conducted to evaluate the prevalence of tick-borne encephalitis virus (TBEV) in wild ticks.

TBE in Slovakia

2019 ◽  
Author(s):  
Jana Kerlik
Keyword(s):  
Encephalitis Virus ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus

The former Czechoslovak Republic was one of the first countries in Europe where the tick-borne encephalitis virus (TBEV) was identified.

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