scholarly journals Antimicrobial Metabolites from Ectomycorrhizal Fungus, Pisolithus tinctorius (Pers.) Coker against Soil Borne Plant Pathogens

2021 ◽  
Vol 108 ◽  
pp. 1-5
Author(s):  
Ganeshkumar P ◽  
◽  
Krishnamoorthy A S ◽  
Sangeetha C ◽  
Nakkeeran S ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Secondary Metabolites ◽  
Plant Pathogens ◽  
Sclerotium Rolfsii ◽  
Ethyl Acetate Fraction ◽  
Macrophomina Phaseolina ◽  
Ectomycorrhizal Fungus ◽  
Pisolithus Tinctorius ◽  
Root Rots ◽  
Antimicrobial Metabolites

Pisolithus tinctorius (Pers.) Coker is an ectomycorrhizal fungus that forms an abundant wealth of biomass in the forest ecosystem, with an untapped treasure of bioactive compounds and secondary metabolites having multifaceted use in health and agrochemical industries. The hidden potential of this fungus is tapped in the current study by using MTP1 isolate of P. tinctorius. The secondary metabolites of P. tinctorius were extracted and tested for their inhibitory efficacy against major soil-borne plant pathogens viz., Fusarium oxysporum f. sp. lycopersici (Sacc.) Synder and Hansen; Macrophomina phaseolina (Goid); Rhizoctonia solani (Kuhn) and Sclerotium rolfsii (Sacc). The cell-free culture (CFC) filtrate of P.tinctorius showed the maximum mycelial inhibition of F. o. f. sp. lycopersici (52.00 %); R. solani (51.92%) and M. phaseolina (48.23%). However, the CFC filtrate mixture did not inhibit the growth of S. rolfsii. The GC-MS analysis of biomolecules composite of ethyl acetate fraction of CFC filtrate indicated the presence of compounds belonging to the nature of fatty acids, aromatic alcohol, and flavonoids. The reduction in the growth of the test pathogens with the increase in the concentration of CFC filtrate and the presence of several unknown compounds in P. tinctorius warrants further study with the purified form of antifungal biomolecules in reducing root rots and wilts incited by the soil-borne phytopathogens.

scholarly journals In-Vitro Efficacy of Trichoderma viride Against Sclerotium rolfsii and Macrophomina phaseolina

2012 ◽  
Vol 4 (4) ◽  
pp. 39-44 ◽  
Author(s):  
Khirood DOLEY ◽  
Paramjit Kaur JITE
Keyword(s):  
Fungal Pathogen ◽  
Crop Production ◽  
Plant Pathogens ◽  
Trichoderma Viride ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina ◽  
Dual Culture ◽  
Human Beings ◽  
Trichoderma Species

The fungal pathogen causes serious widespread losses to agricultural crops worldwide. Therefore, economy of countries may worsen especially of developing countries. In addition, harmful chemical pesticides which are being used today for increasing crop production creates very serious health hazardous problems to human beings and ecosystem as a whole. The antagonistic potential of Trichoderma species which has been long known to control various soil-borne fungal pathogens in biological way may be utilized. The faster growth rates with which it competes with fungal pathogen mainly brings upon their antagonistic characteristics. An investigation was carried out in laboratory condition towards biological efficacy of T. viride on potato dextrose agar (PDA) medium for the bio-control of soil-borne plant pathogens Sclerotium rolfsii and Macrophomina phaseolina in in-vitro condition. The dual culture technique was followed in which T. viride showed significant antifungal activities towards both the pathogens. T. viride significantly inhibited the mycelial radial growth of S. rolfsii by 75% and M. phaseolina by 71.42%. The results showed variable mycelial growth rate for all fungal isolates which was determined after 6 days of incubation in which T. viride showed minimum of 4.00 days to completely cover the petri-plates and S. rolfsii showed 4.33 days whereas M. phaseolina showed 6.33 days. Thus, T. viride showed encouraging results regarding their biopesticidal and biofungicidal potential against plant pathogens which may be endorsed to substitute harmful chemical supplements that exists in modern day agricultural practices.

Characterization of major secondary metabolites produced in soilless mix by a formulated strain of the biocontrol fungus Gliocladium virens

1992 ◽  
Vol 38 (12) ◽  
pp. 1274-1280 ◽  
Author(s):  
R. D. Lumsden ◽  
C. J. Ridout ◽  
M. E. Vendemia ◽  
D. J. Harrison ◽  
R. M. Waters ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Plant Pathogens ◽  
Sclerotium Rolfsii ◽  
Pythium Ultimum ◽  
Potting Medium ◽  
Gliocladium Virens ◽  
Individual Bands ◽  
Layer Chromatography

A strain of Gliocladium virens (GL-21 = G-20) formulated in alginate prill was grown for 3 days in a commercial soilless potting medium. Chloroform extractions were separated by thin-layer chromatography and individual bands from G-20 cultures in soilless medium were identified by standards, mass spectrometry, and nuclear magnetic resonance as (i) a mixture of fatty acids, (ii) viridin, (iii) gliotoxin, (iv) dimethylgliotoxin, (v) viridiol, and (vi) a mixture of phenolics including ferulic acid. None of these compounds was detected in the medium not amended with prills of isolate G-20 of G. virens. Other isolates of G. virens, including G-3 and G-9, produced gliovirin and did not produce gliotoxin. Of all the metabolites produced by G-20 in soilless medium, only gliotoxin strongly inhibited germination of sporangia and growth of Pythium ultimum (growth prevention at 1.0 μg/mL), growth of Rhizoctonia solani mycelium (0.5 μg/mL), or germination of sclerotia and growth of Sclerotium rolfsii (50 μg/mL). Viridin was more inhibitory to growth of R. solani (1.0 μg/mL, minimum inhibitory concentration) than to P. ultimum (25 μg/mL) or S. rolfsii (50 μg/mL). Growth of G. virens isolate G-20 was inhibited by 25 μg/mL of viridin but not by 100 μg/mL gliotoxin. In contrast, growth of isolate G-3 was inhibited by 50 μg/mL gliotoxin but not by 100 μg/mL of viridin. Dimethylgliotoxin, fatty acids, and phenolics were not inhibitory to any of the fungi tested. These results suggest that gliotoxin is the major antibiotic metabolite inhibitory to Pythium and Rhizoctonia of this formulated strain of G. virens. This information will be useful for monitoring metabolite production by G. virens, determining optimum gliotoxin production in situ, and improving strain performance for biocontrol of plant pathogens. Key words: antibiotic, biological control, antagonist.

Mycoparasitism of sclerotial fungi by Teratosperma oligocladum

1981 ◽  
Vol 27 (9) ◽  
pp. 886-892 ◽  
Author(s):  
W. A. Ayers ◽  
P. B. Adams
Keyword(s):  
Plant Pathogens ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina ◽  
Soil Extract ◽  
Natural Soil ◽  
Sclerotium Cepivorum ◽  
Moist Sand ◽  
Parasitic Activity ◽  
Common Laboratory

Sclerotia of Sclerotinia minor were parasitized by Teratosperma oligocladum, a recently described dematiaceous hyphomycete. The mycoparasite was cultured on living sclerotia placed on water agar and on sclerotia in moist sand. It grew poorly on several common laboratory media but growth in vitro was enhanced by supplements of soil extract and, especially, by aqueous extracts of sclerotia. Sclerotia of S. minor, S. sclerotiorum, S. trifoliorum, Sclerotium cepivorum, and Botrytis cinerea were parasitized in vitro, but sclerotia of Sclerotium rolfsii and Macrophomina phaseolina were not. Macroconidia of T. oligocladum germinated on membrane filters placed on soil containing sclerotia of S. minor but not on soil without sclerotia. Sclerotia of three Sclerotinia spp. were infected within 2 weeks in soil infested with the mycoparasite. Teratosperma oligocladum parasitized and destroyed all of the sclerotia of S. minor buried in a natural soil by 10 weeks. Parasitism was equally good at 20 and 25 °C, but occurred more slowly at 15 °C. No parasitic activity occurred at 30 °C. The morphology, cultural characteristics, and mycoparasitic habit of T. oligocladum indicated that it was similar in many respects to the mycoparasite, Sporidesmium sclerotivorum, and that it is a potentially useful agent for the biological control of sclerotial plant pathogens.

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

scholarly journals QUANTITATIVE ANALYSIS OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. LEAF EXTRACT

2016 ◽  
pp. 183
Author(s):  
Parameswari P ◽  
Devika Rengaswamy
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Quantitative Analysis ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Metabolic Reaction ◽  
Quantitative Investigation ◽  
Artemisia Nilagirica ◽  
Quantitative Examination

<p>ABSTRACT<br />Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely<br />on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on<br />different human diseases.<br />Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments<br />for quantitative investigation of phytochemicals according to standard strategies.<br />Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg<br />terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows<br />separately.<br />Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward<br />extraction and refinement of specific bioactive mixes for human nourishment.<br />Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.</p>

scholarly journals Bioactive Secondary Metabolites of the Genus Diaporthe and Anamorph Phomopsis from Terrestrial and Marine Habitats and Endophytes: 2010–2019

2021 ◽  
Vol 9 (2) ◽  
pp. 217
Author(s):  
Tang-Chang Xu ◽  
Yi-Han Lu ◽  
Jun-Fei Wang ◽  
Zhi-Qiang Song ◽  
Ya-Ge Hou ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Small Molecules ◽  
Host Plants ◽  
Plant Pathogens ◽  
Bioactive Metabolites ◽  
Drug Candidates ◽  
The Past ◽  
Marine Habitats ◽  
Bioactive Secondary Metabolites ◽  
Unidentified Species

The genus Diaporthe and its anamorph Phomopsis are distributed worldwide in many ecosystems. They are regarded as potential sources for producing diverse bioactive metabolites. Most species are attributed to plant pathogens, non-pathogenic endophytes, or saprobes in terrestrial host plants. They colonize in the early parasitic tissue of plants, provide a variety of nutrients in the cycle of parasitism and saprophytism, and participate in the basic metabolic process of plants. In the past ten years, many studies have been focused on the discovery of new species and biological secondary metabolites from this genus. In this review, we summarize a total of 335 bioactive secondary metabolites isolated from 26 known species and various unidentified species of Diaporthe and Phomopsis during 2010–2019. Overall, there are 106 bioactive compounds derived from Diaporthe and 246 from Phomopsis, while 17 compounds are found in both of them. They are classified into polyketides, terpenoids, steroids, macrolides, ten-membered lactones, alkaloids, flavonoids, and fatty acids. Polyketides constitute the main chemical population, accounting for 64%. Meanwhile, their bioactivities mainly involve cytotoxic, antifungal, antibacterial, antiviral, antioxidant, anti-inflammatory, anti-algae, phytotoxic, and enzyme inhibitory activities. Diaporthe and Phomopsis exhibit their potent talents in the discovery of small molecules for drug candidates.

scholarly journals Secondary metabolites from Diplodia maydis and Sclerotium rolfsii with antibiotic activity

2006 ◽  
Vol 37 (1) ◽  
pp. 14-16 ◽  
Author(s):  
Marisa Alves Nogueira ◽  
Gaspar Diaz ◽  
William Andrioli ◽  
Fabiana André Falconi ◽  
José Renato Stangarlin
Keyword(s):  
Secondary Metabolites ◽  
Sclerotium Rolfsii ◽  
Antibiotic Activity

scholarly journals AGERATUM CONYZOIDES L. (PEMANFAATAN SEBAGAI OBAT DAN BIOAKTIVITASNYA)

2019 ◽  
Vol 11 (3) ◽  
pp. 197
Author(s):  
Marina Silalahi
Keyword(s):  
Fatty Acids ◽  
Secondary Metabolites ◽  
Traditional Medicine ◽  
Wild Plants ◽  
Ageratum Conyzoides ◽  
Comprehensive Information

ABSTRACTAgeratum conyzoides or badotan is a wild plants which uses as traditional medicine. This plant has a distinctive aroma similar to "the smell of goats" so it is called "goatweed". This article aims to explain the use of A. conyzoides as a drug and its bioactivity. The writing of this article is based on the study of literature obtained online and offline including various scientific articles then reviewed and synthesized so as to provide comprehensive information regarding the use of A. conyzoides as traditional medicine. In traditional medicine, Ageratum conyzoides is used as medicine for wounds, ulcers, and fever. Ageratum conyzoides have secondary metabolites such as terpenoids, flavonoids, steroids, terpenes, saponins, fatty acids, and alkaloids, with the main compounds stigmasterol and β-sitosterol. Bioactivity of A. conyzoides are antihistamine, antimicrobial, antiplasmodial, cytoprotective, analgesic, antioxidant and anti diabetes mellitus.Keywords: Ageratum conyzoides, antimicrobial, β-sitosterol, cytoprotective, analgesic. ABSTRAKAgeratum conyzoides atau badotan merupakan salah satu tumbuhan liar yang banyak dimanfaatkan sebagai obat tradisional. Tumbuhan ini memiliki aroma khas mirip dengan “bau kambing” sehingga disebut juga sebagai “goatweed”. Artikel ini bertujuan untuk menjelaskan pemanfaatan A. conyzoides sebagai obat dan bioaktivitasnya. Penulisan artikel ini didasarkan pada kajian literatur yang diperoleh secara online maupun offline meliputi berbagai artikel ilmiah kemudian dikaji dan disintesakan sehingga memberikan informasi yang kompehensif mengenai pemanfaatan A. conyzoides sebagai obat tradisional. Dalam pengobatan tradisional Ageratum conyzoides dimanfaatkan sebagai obat luka, bisul, dan demam. Ageratum conyzoides mengandung metabolit sekunder dari golongan terpenoid, flavonoid, steroid, terpen, senyawa, saponin, asam lemak, dan alkaloid, dengan senyawa utama stigmasterol dan β‐sitosterol. Bioaktivitas yang dimiliki oleh A. conyzoides antara lain sebagai anti histamin, antimikroba, antiplasmodial, sitoprotektif, analgesik, antioksidan dan anti diabetes mellitus.Kata Kunci : Ageratum conyzoides, antimikroba, β‐sitosterol, sitoprotektif, analgesik.

Transcriptomic response ofRalstonia solanacearumto antimicrobialPseudomonas fluorescensSN15-2 metabolites

2018 ◽  
Vol 64 (11) ◽  
pp. 816-825 ◽  
Author(s):  
Haibo Lou ◽  
Xiaobing Wang ◽  
Jun Chen ◽  
Bozhi Wang ◽  
Wei Wang
Keyword(s):  
Oxidative Stress ◽  
Plant Pathogens ◽  
Molecular Mechanisms ◽  
Acid Metabolism ◽  
Iron Absorption ◽  
Fermentation Broth ◽  
Transcriptomic Response ◽  
Transmission Electron ◽  
Antimicrobial Metabolites ◽  
Tomato Wilt

To develop efficient biocontrol agents, it is essential to investigate the response of soil-borne plant pathogens to such agents. For example, the response of Ralstonia solanacearum, the tomato wilt pathogen, to antimicrobial metabolites of Pseudomonas fluorescens is unknown. Thus, we assessed the effects of P. fluorescens SN15-2 fermentation broth on R. solanacearum by transmission electron microscopy and transcriptome technology. RNA sequencing identified 109 and 155 genes that are significantly upregulated and downregulated, respectively, in response to P. fluorescens metabolites, many of which are associated with the cell membrane and cell wall, and with nucleotide acid metabolism, iron absorption, and response to oxidative stress. This study highlights the effectiveness of P. fluorescens metabolites against the tomato wilt pathogen and helps clarify the underlying molecular mechanisms.

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