scholarly journals Susceptibility of Pseudomonas aeruginosa isolated from urine to some antibiotics

2011 ◽  
Vol 10 (2) ◽  
pp. 86 ◽  
Author(s):  
H.Ali Salih , M. Abdulbary And A.S. Abdulrida
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistant ◽  
High Concentration ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Almost All ◽  
Chronic Uti ◽  
Urine Specimens

Pseudomonas aeruginosa is a member of genus Pseudomonas that it is Gram – negative , glucose - nonfermenting aerobic rod , isolated from chronic UTI . It is resistant to high concentration of salts and dyes , weak antiseptics and commonly used antibiotics. Because of the increasing in drug – resistant of strains of P. aeruginosa especially that which isolated from UTI, this study was done among the patients attending hospitals in Al-Najaf city during January to December 2009 to maintain the susceptibility pattern of organism isolated from urine specimens. A total 72(100%) samples of P. aeruginosa were isolated from urine specimens of patients. The bacteria isolated were identified by colony morphology , microscopy and relevant biochemical tests. Antimicrobial sensitivity pattern was tested using standard guidelines. Almost all of the P. aeruginosa isolates were sensitive to Amikacin(100%) , Norfloxacin(86% ( Ciprofloxacin(83 %) & Tobramycin(83%) but highly resistant to Doxycycline

scholarly journals Pola Sensitivitas Isolat Klinis Bakteri Gram Negatif Multiresisten Obat Terhadap Fosfomisin dari Beberapa Rumah Sakit Kota Pekanbaru

2019 ◽  
Vol 13 (1) ◽  
pp. 28
Author(s):  
Dewi Anggraini ◽  
Atya Nasmah ◽  
Fauzia Andrini
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Clinical Isolate ◽  
Alternative Therapy ◽  
Drug Resistant ◽  
Microbiology Laboratory ◽  
Cross Sectional ◽  
Gram Negative ◽  
Sensitivity Pattern

Over the last few years, there has been a decrease in amount of effective antibiotics, coincide with the lack of newantibiotics relevant for the treatment of Gram negative multi-drug resistant organisms infections. This problem can beovercome by the assessment and re-introduction of old antibiotics such as fosfomycin. Fosfomycin is a broad spectrumantibiotic that is bactericidal. This study aimed to find out the sensitivity pattern of clinical isolate multi-drug resistantGram negative bacteria to fosfomycin with descriptive cross sectional method. The sample used in this study wasclinical isolates collection of Microbiology Laboratory Faculty of Medicine, University of Riau from Hospitals inPekanbaru. The result showedsensitivity to fosfomycin Escherichia coli producing ESBL (88%), Klebsiella pneumoniaeproducing ESBL (95%), Acinotobacter baumannii resistant meropenem (27%), Pseudomonas aeruginosa resistantmeropenem (36%) and K.pneumoniae resistant meropenem (70%). Fosfomycin can be used as alternative therapy forinfections caused by Gram-negative Multi-drug resistant organisms.

scholarly journals ANTIBIOTIC SENSITIVITY PATTERN OF BACTERIA ISOLATED FROM THE ENVIRONMENT OF INTENSIVE CARE UNIT OF WAD MEDANI EMERGENCY HOSPITAL, GEZIRA STATE, SUDAN

2020 ◽  
pp. 82-85
Author(s):  
WALEED ELSIDDIG MOHAMMED ◽  
HASSABELRASOUL ELFADIL HASSAN ◽  
MIRGHANI ABDELRHMAN YOUSIF
Keyword(s):  
Intensive Care Unit ◽  
Pseudomonas Aeruginosa ◽  
Intensive Care ◽  
High Resistance ◽  
Bacterial Contamination ◽  
Biochemical Tests ◽  
Coagulase Negative Staphylococci ◽  
Gram Negative ◽  
Emergency Hospital ◽  
Sensitivity Pattern

Objective: The objective of the present study was primarily to identify the sources and types of bacterial contamination associated with hospital-acquired infections in the intensive care unit and to investigate the sensitivity pattern of isolated bacteria to prescribed antibiotics of Wad Medani Emergency Hospital, Gezira State, Sudan. Methods: A total of 50 swab samples were obtained from 14 different sites, including inanimate objects as well as nurses’ hands in the ICU. Identification of the bacterial isolates was performed utilizing Gram’s staining test and standard biochemical tests; likewise, the respective antimicrobial sensitivity was determined based on the guidelines recommended by the Clinical and Laboratory Standards Institute (CLSI). Results: Showed the prevalence of Gram-positive isolates as Coagulase-negative staphylococci (30%), Staphylococcus aureus (20%), Bacillus spp (15%), and Streptococcus spp (4%). On the other hand, the Gram negative isolates were: Pseudomonas aeruginosa (11%), \Kliebsiella pneumoniae (7%), Proteus mirabilis (5%), and Enterobacter spp.(5%). Floor, Monitors, Patients’ oxygen masks and infusion-stands as well as nurses’ hands, were the most contaminated sites. Staphylococci showed a reasonable sensitivity response to Gentamicin and Vancomycin and high resistance to Erythromycin and Co-trimoxazole; whereas Gram-negative isolates showed high resistance to first and second-generation Cephalosporins and demonstrated good sensitivity pattern to Gentamicin and Meropenem. Pseudomonas aeruginosa also showed reasonable sensitivity to Ciprofloxacin. Conclusion: Findings of the study demonstrated high bacterial contamination levels in ICU. 

scholarly journals Infections by Pseudomonas aeruginosa and Antibiotic Resistance Pattern of the Isolates from Dhaka Medical College Hospital

2016 ◽  
Vol 1 (2) ◽  
pp. 48-51 ◽  
Author(s):  
Aliya Rashid ◽  
Akhtaruzzaman Chowdhury ◽  
Sufi HZ Rahman ◽  
Shahin Ara Begum ◽  
Naima Muazzam
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Significant Proportion ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
Biochemical Tests ◽  
College Hospital ◽  
Clinical Specimens ◽  
Medical College ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern

Pseudomonas aeruginosa is one of the most common gram-negative microorganisms identified in the clinical specimens of hospital admitted patients. This was a retrospective study done among the patients attending Dhaka Medical College Hospital during January to December, 2006. A total 294 strains of P. aeruginosa were isolated of which 206 (70%) were from admitted patients and 88 (30%) from outpatients. Some 175 (60%) of the isolates were from males while 119 (40%) from females. Majority of the patients (141, 41.2%) were aged between 21-40 years and 102 (34.7%) were below 20 years. A significant proportion (about 45%) of the patients were from casualty wards, surgical outdoor, ear-nose-throat outdoor and burn unit. Four of the clinical specimens including wound swab, pus, aural swab and urine comprised of 279 (95%) of the total samples. The bacteria isolated were identified by colony morphology, microscopy and relevant biochemical tests. Antimicrobial sensitivity pattern was tested using standard guidelines. Almost all of the P. aeruginosa isolates were resistant to cefixime (93.3%) and co-trimoxazole (93.5%), majority were resistant to ceftazidime (86.8%), gentamycin (77.3%) and ciprofloxacin (75.5%). The result of the study shows that imipenem is the most effective drugagainst P. aeruginosa, followed by amikacin and ciprofloxacin.Bangladesh J Med Microbiol 2007; 01 (02): 48-51

scholarly journals Molecular Epidemiology of Multi-Drug Resistant Pseudomonas aeruginosa Isolates from Hospitalized Patients in Greece

2020 ◽  
Vol 8 (11) ◽  
pp. 1652
Author(s):  
Olga Pappa ◽  
Anastasia Maria Kefala ◽  
Kyriaki Tryfinopoulou ◽  
Marios Dimitriou ◽  
Kostas Kostoulas ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Epidemiology ◽  
Financial Resources ◽  
Drug Resistant ◽  
Hospital Acquired Infections ◽  
Antimicrobial Sensitivity ◽  
Tertiary Hospitals ◽  
Hospital Acquired ◽  
First Time ◽  
Hospital Transmission

Resistant Pseudomonas aeruginosa isolates are one of the major causes of both hospital-acquired infections (HAIs) and community-acquired infections (CAIs). However, management of P. aeruginosa infections is difficult as the bacterium is inherently resistant to many antibiotics. In this study, a collection of 75 P. aeruginosa clinical isolates from two tertiary hospitals from Athens and Alexnadroupolis in Greece was studied to assess antimicrobial sensitivity and molecular epidemiology. All P. aeruginosa isolates were tested for susceptibility to 11 commonly used antibiotics, and the newly introduced Double Locus Sequence Typing (DLST) scheme was implemented to elucidate the predominant clones. The tested P. aeruginosa isolates presented various resistant phenotypes, with Verona Integron-Mediated Metallo-β-lactamase (VIM-2) mechanisms being the majority, and a new phenotype, FEPR-CAZS, being reported for the first time in Greek isolates. DLST revealed two predominant types, 32-39 and 8-37, and provided evidence for intra-hospital transmission of the 32-39 clone in one of the hospitals. The results indicate that DLST can be a valuable tool when local outbreaks demand immediate tracking investigation with limited time and financial resources.

scholarly journals Study of Helicobacter hepaticus in gallbladders with cholelithiasis and its sensitivity pattern

1970 ◽  
Vol 7 (2) ◽  
pp. 125-128 ◽  
Author(s):  
SB Pradhan
Keyword(s):  
Stone Formation ◽  
Gallbladder Disease ◽  
Health Care Expenditure ◽  
College Teaching ◽  
Helicobacter Hepaticus ◽  
High Concentration ◽  
Biochemical Tests ◽  
Silver Stain ◽  
Fixed Tissue ◽  
Sensitivity Pattern

Background: Cholelithiasis is one of the most common digestive surgical disorders. The natural history of gallstone development is unknown. Bacteria are found in high concentration in bile and stone. It is difficult to ascertain whether bacterial infection of the bile arose before stone formation or vice versa. Materials & methods: Prospective study was carried out in 30 cholecystectomy specimens of patients with cholelithiasis collected from 15th January 2005 to 24th July 2005 in Department of Pathology, Kathmandu Medical College Teaching Hospital. The samples were collected in a sterile vial containing 0.5% saline. A small piece of gallbladder was taken in Blood agar and Chocolate agar media for micro anaerobic culture at 370C. The growth of the organism was observed after 48 hours of incubation. Gram stain and various biochemical tests were performed for the confirmation of Helicobacter hepaticus and its sensitivity pattern was studied. Histologically Helicobacter hepaticus was demonstrated in formalin fixed tissue sections using Warthin Starry Silver stain. Results: A total of 76.66% cases showed growth positivity. Nitrofurantoin was found to be the most sensitive drug (95.7%) for Helicobacter hepaticus followed by Ciprofloxacin (91.3%), Cephalaxin (91.3%), Certriaxone (91.3%), Ofloxacin (82.6%), Amikacin (65.2%) and Norfloxacin (60.9%). Helicobacter hepaticus was least sensitive to Amoxycillin (57%). Conclusion: From this study, it is quite apparent that Helicobacter hepaticus infection is commonly associated (76.66%) with cholelithiasis. If the patients are treated with the sensitive antibiotics regularly and the infection, that is the number one cause for the precipitation of bile leading to the formation of gallstones, can be controlled, the incidence of cholelithiasis can be reduced so that the health care expenditure related to gallbladder disease can be reduced markedly. Key words: Cholelithiasis Helicobacter hepaticus; WSS stain DOI: 10.3126/kumj.v7i2.2704 Kathmandu University Medical Journal (2009) Vol.7, No.2 Issue 26, 125-128

scholarly journals Draft Genome Sequence of a Pseudomonas aeruginosa Strain Able To Decompose N , N -Dimethyl Formamide

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lingyue Yan ◽  
Ming Yan ◽  
Lin Xu ◽  
Li Wei ◽  
Liting Zhang
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Genome Sequence ◽  
Draft Genome ◽  
Carbon Sources ◽  
Draft Genome Sequence ◽  
Dimethyl Formamide ◽  
Organic Chemicals ◽  
Negative Bacterium ◽  
High Concentration ◽  
Gram Negative

Pseudomonas aeruginosa is a Gram-negative bacterium, which uses a variety of organic chemicals as carbon sources. Here, we report the genome sequence of the Cu1510 isolate from wastewater containing a high concentration of N , N -dimethyl formamide.

Microbial Quality of Drinking Water in Some Finnish Distribution Systems

1993 ◽  
Vol 27 (3-4) ◽  
pp. 151-154 ◽  
Author(s):  
Kirsti Lahti
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Surface Water ◽  
Distribution Systems ◽  
Heterotrophic Bacteria ◽  
Biochemical Tests ◽  
Gram Negative ◽  
Total Coliforms ◽  
Groundwater Supply ◽  
Sampling Points ◽  
Faecal Streptococci

The occurrence of heterotrophic bacteria, Pseudomonas aeruginosa, Aeromonas, total coliforms, faecal streptococci, molds and yeasts in distribution systems of two surface water, two artificial groundwater and two groundwater supplies was examined three times during one year. All water samples studied were free of Pseudomonas aeruginosa and faecal streptococci in 100 ml. Total coliforms were detected in water distributed from one groundwater supply. Aeromonas hydrophila/caviae in the concentration range <1 - 32 cfu/100 ml also occurred in distributed water from the same supply. Molds and yeasts were frequently found in piped water from all watenvorks. The concentration of molds exceeded 100 cfu/100 ml in two sampling points. The differences in heterotrophic plate counts were greater between different sampling points of the same surface water supplies than between waterworks with different raw water origins. Over 80 % of the isolated strains of heterotrophic bacteria from different sampling locations were gram-negative and 57 % were oxidase-negative. The identified gram-negative strains belonged to the genera Pseudomonas, Moraxella, Alcaiigenes and Flavobacterium. The majority of strains (64 %) remained unidentified with the biochemical tests used.

scholarly journals Bacteriological Spectrum and Antimicrobial Sensitivity of Pathogens In Children with Hematologic Malignancies and Febrile Neutropenia Research

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Anahita Sanaei Dashti ◽  
Jabarali Safaei ◽  
Fariba Shirvani ◽  
Mohammad Rahim Kadivar ◽  
Erfan Taherifard
Keyword(s):  
Febrile Neutropenia ◽  
Hematologic Malignancies ◽  
Cross Sectional Study ◽  
Blood Cultures ◽  
Coagulase Negative Staphylococci ◽  
Cross Sectional ◽  
Gram Negative ◽  
Antimicrobial Sensitivity ◽  
Demographic Questionnaire ◽  
Sensitivity Pattern

Objectives: This study aimed to investigate the spectrum of bacterial pathogens and their antimicrobial sensitivity in pediatric patients admitted with febrile neutropenia (FN) and hematologic malignancies. Methods: This prospective, cross-sectional study was carried out on 65 children (60% boys, mean age 7.3 5.3 years) with hematologic malignancies and FN hospitalized at the Amir Hematology and Oncology Hospital, Shiraz, Iran, between December 2018 and May 2019. Samples were taken from blood, nose, axilla, and inguinal area (NAI). NAI cultures were repeated after 72 hours of admission, and blood culture was repeated if needed. A demographic questionnaire on age, sex, and admission time was filled out for each patient. Results: The most common grown bacteria in the NAI samples were coagulase-negative staphylococci (CoNS). Second cultures from these sites revealed nosocomial contamination. Eighteen patients (27.7%) had positive blood cultures, with 61.1 % of the isolated pathogens being Gram-negative (mostly Escherichia coli and Pseudomonas aeroginosa) and 38.9% Gram-positive. The blood cultures results did not match those of the NAI cultures in 61.1% of the patients. About one-fourth of the Gram-negative pathogens isolated from blood were resistant to cefepime, the main empirical antibiotic for FN management at our center. Conclusions: Gram-negative bacteria are predominant organisms in pediatric FN patients at our center, most resistant to cefepime. If a similar antimicrobial sensitivity pattern is observed in future studies, a change in local guidelines might be considered for FN management.

scholarly journals Antimicrobial sensitivity pattern from clinical isolates at a tertiary care teaching hospital of rural Bengal: a pilot study

2019 ◽  
Vol 8 (6) ◽  
pp. 1196
Author(s):  
Tapashi Ghosh ◽  
Sabyasachi Saha ◽  
Ananya Mandal ◽  
Nikhil Tudu ◽  
Jayanta Bikash De
Keyword(s):  
Blood Culture ◽  
Clinical Isolates ◽  
Prescribing Patterns ◽  
Gram Positive ◽  
Gram Negative ◽  
Wound Swab ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
The Common ◽  
Gram Negative Organisms

Background: Antimicrobial sensitivity pattern from clinical isolates can reveal important information that can help in drafting the hospital antibiotic policy as well as help improve prescribing patterns and patient outcome in a particular region.Methods: Data from the results of the antimicrobial sensitivity pattern of clinical isolates of the patients between 1stJuly and 31st December 2018 were collected on a pre-designed and pre tested case study form and analysed with the help of descriptive statistics.Results: A total of 75 blood culture reports were obtained which showed 58 gram positive cultures. Further 46 of the gram positive samples were positive for Coagulase negative Staphylococcus. A total of 305 urine samples were obtained for culture which showed gram negative cultures. Paediatric and medicine wards were the common yielding sites. A total of 242 pus reports were obtained which showed 47 gram positive cultures. A total of 154 wound swab samples were obtained which showed 47 gram positive cultures. For pus and wound swab samples, surgery wards were the common yielding sites. Common gram negative organisms seen were Klebsiella sp., E. coli, Citrobacter sp., Pseudomonas, Proteus and Enterobacter. Gram positive organisms were commonly resistant to Erythromycin, orally active Penicillins, Vancomycin and Teicoplanin and gram negative organisms were commonly resistant to Cephalosporins, Aminoglycosides, Colistin, Fluroquinolones and Meropenem.Conclusions: This study showed that over six months samples of body pus, wound swab, blood culture and urine showed high levels of resistance to commonly used antibiotics. This would provide an outline for development of an effective hospital Infection Control Policy.

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