scholarly journals Root rot toxines as a factor in the selection of resistant forms of oats in vitro

2020 ◽  
Author(s):  
S. Yu. Lugovtsova ◽  
N. A. Neshumaeva ◽  
V. Yu. Stupko ◽  
N. V. ZobovaN.V.
Keyword(s):  
Culture Filtrate ◽  
Root Rot ◽  
Selective Media ◽  
Fusarium Mycotoxin ◽  
Selection Of

The oat regenerants with resistance to the Fusarium mycotoxin influence were obtained in vitro on selective media with different concentrations of root rot culture filtrate.

scholarly journals OPTIMIZATION OF IN VITRO SELECTIVE MEDIA FOR SELECTING ANTHRACNOSIS-RESISTANT FLAX CELLS

2021 ◽  
Author(s):  
Н.В. Пролётова ◽  
Л.П. Кудрявцева
Keyword(s):  
Culture Filtrate ◽  
In Vitro Selection ◽  
Selective Medium ◽  
Selective Media ◽  
Primary Roots ◽  
Morphogenic Callus ◽  
Weakly Virulent ◽  
Germinated Seeds ◽  
Selection Of

Цель исследований – оптимизация селективных сред для проведения отбора in vitro каллусных клеток льна, устойчивых к культуральному фильтрату штаммов возбудителя антракноза и создание in vitro новых генотипов, устойчивых к болезни. В результате исследований уточнен состав культурального фильтрата штаммов антракноза. Выявлено, что токсичность культуральных фильтратов не зависела от вирулентности используемых штаммов – более токсичными оказались культуральные фильтраты штаммов 784 (сильновирулентного) и 780 (средневирулентного) (загнивание и отмирание первичных корешков на 5 сутки наблюдали у 67 – 88% проросших семян), менее токсичны – штаммы 793 (сильновирулентный) и 788 (слабовирулентный) (на 5 сутки загнивание и отмирание первичных корешков отмечено у 9 – 15% проросших семян). Установлено, что морфогенные очаги формировались активнее у генотипов, морфогенный каллус которых переносили на среду с аналогичной или более высокой концентрацией культурального фильтрата. Показано, что на 14 сутки во втором пассаже с большей частотой формировались морфогенные каллусы, почки и побеги при использовании в первом и втором пассажах селективной среды, содержащей культуральный фильтрат в концентрации 40 мл/л, или в первом пассаже – 40 мл/л, а во втором – 44 мл/л. Выделены генотипы, сохраняющие устойчивость к антракнозу в течение трёх поколений на уровне 50 – 60%: НО-78 х Ленок, HJI-103-2 х Ленок, НЛ-40-1 х Ленок, HЭ-38 х Росинка, НЭ-36 х Ленок, НЭ-17 х Ленок, HЭ-16-2 х Росинка. Research objective – optimization of selective media for in vitro selection of flax callus cells resistant to culture filtrate of anthracnose pathogen strains and in vitro creation of new disease-resistant genotypes. As a result of the research, the composition of the culture filtrate of anthracnose strains was clarified. It was revealed that the toxicity of cultural filtrates did not depend on the virulence of the strains used - cultural filtrates of strains 784 (highly virulent) and 780 (medium virulent) turned out to be more toxic (decay and death of primary roots on day 5 was observed in 67 - 88% of germinated seeds), less toxic - strains 793 (strongly virulent) and 788 (weakly virulent) (on the 5th day, decay and death of primary roots was noted in 9-15% of germinated seeds). It was found that morphogenic foci were formed more actively in genotypes, the morphogenic callus of which was transferred to a medium with a similar or higher concentration of the culture filtrate. It was shown that on the 14th day in the second passage, morphogenic callus, buds and shoots were formed with a greater frequency when using in the first and second passages a selective medium containing a culture filtrate at a concentration of 40 ml/l, or in the first passage - 40 ml/l, and in the second - 44 ml/l. Genotypes were identified that retain resistance to anthracnose for three generations at a level of 50 - 60%: NO-78 x Lenok, HJI-103-2 x Lenok, NL-40-1 x Lenok, NE-38 x Rosinka, NE-36 x Lenok, NE-17 x Lenok, NE-16-2 x Rosinka.

IN VITRO SELECTION OF APPLE ROOTSTOCK SOMACLONES WITH PHYTOPHTHORA CACTORUM CULTURE FILTRATE

1990 ◽  
pp. 409-416 ◽  
Author(s):  
P. ROSATI ◽  
B. MEZZETTI ◽  
M. ANCHERANI ◽  
S. FOSCOLO ◽  
S. PREDIERI ◽  
...  
Keyword(s):  
Culture Filtrate ◽  
In Vitro Selection ◽  
Phytophthora Cactorum ◽  
Apple Rootstock ◽  
Selection Of

scholarly journals Mefenoxam Sensitivity, Aggressiveness, and Identification of Pythium Species Causing Root Rot on Floriculture Crops in North Carolina

Plant Disease ◽  
2015 ◽  
Vol 99 (11) ◽  
pp. 1550-1558 ◽  
Author(s):  
E. C. Lookabaugh ◽  
K. L. Ivors ◽  
B. B. Shew
Keyword(s):  
North Carolina ◽  
Host Species ◽  
Root Rot ◽  
Internal Transcribed Spacer ◽  
Ornamental Plants ◽  
Pythium Species ◽  
Selective Media ◽  
Microtiter Plates ◽  
Pythium Root Rot

Herbaceous ornamental plants exhibiting symptoms of Pythium root rot were collected from 26 greenhouses in 21 counties in North Carolina (NC) from 2010 to 2012. Plant symptoms ranged from mild stunting to severe wilting, root rot, and death. Roots were plated on selective media, and 356 isolates of Pythium were recovered from 34 host species. Selected isolates were identified by sequencing of the internal transcribed spacer (ITS) rDNA gene region. Seventeen Pythium species were identified, with P. aphanidermatum, P. irregulare, and P. myriotylum comprising 75% of the 320 isolates sequenced. Twelve of the 26 greenhouses had more than one species present. Mefenoxam sensitivity was tested in vitro by growing isolates in wells of microtiter plates containing clarified V8 agar amended with 100 µg a.i./ml mefenoxam. Colonization was scored after 24 to 48 h using a scale of 0 (no growth) to 5 (entire well colonized). Fifty-two percent of the isolates were resistant to mefenoxam (mean score ≥4). All 32 isolates of P. myriotylum were sensitive, whereas sensitivity varied among isolates of P. aphanidermatum and P. irregulare. Resistant and sensitive isolates of the same species were found within the same greenhouses. The aggressiveness of P. aphanidermatum and P. irregulare isolates was evaluated on poinsettia, Gerbera daisy, and petunia. P. aphanidermatum was more aggressive than P. irregulare on poinsettia and petunia; symptoms were mild and no differences in aggressiveness were observed on Gerbera daisy. Sensitivity to mefenoxam was not related to aggressiveness.

In Vitro Cell Selection of Sugar Beet for Resistance to Culture Filtrate of the Fungus Fusarium oxysporum

2019 ◽  
Vol 53 (4) ◽  
pp. 307-314
Author(s):  
R. S. Yerzhebayeva ◽  
A. M. Abekova ◽  
G. H. Bersimbaeva ◽  
K. T. Konysbekov ◽  
S. O. Bastaubaeva ◽  
...  
Keyword(s):  
Sugar Beet ◽  
Fusarium Oxysporum ◽  
Culture Filtrate ◽  
Cell Selection ◽  
Selection Of
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