In vitro selection of an effective fungicide againstArmillaria mellea and control of white root rot of grapevine in the field

2006 ◽  
Vol 62 (3) ◽  
pp. 223-228 ◽  
Author(s):  
Olga Aguín ◽  
J Pedro Mansilla ◽  
María J Sainz
Keyword(s):  
Root Rot ◽  
In Vitro Selection ◽  
White Root Rot ◽  
And Control ◽  
Selection Of

scholarly journals Keefektifan Oligochitosan dalam Menekan Pertumbuhan Jamur Patogen Rigidoporus lignosus [(Klotzsch) Imazeki] Penyebab Penyakit Jamur Akar Putih pada Tanaman Cengkeh secara in Vitro

Agrikultura ◽  
2016 ◽  
Vol 27 (1) ◽  
Author(s):  
Fitri Widiantini ◽  
Andang Purnama ◽  
Endah Yulia ◽  
Dwindry Formanda
Keyword(s):  
Root Rot ◽  
Mycelial Growth ◽  
White Root Rot ◽  
Root Rot Disease ◽  
Randomized Design ◽  
Negative Effect ◽  
Rigidoporus Lignosus ◽  
Rot Disease ◽  
And Control

ABSTRACTThe effectiveness of Oligochitosan in Suppressing the Growth of Fungal PathogenRigidoporus lignosus [(Klotzsch) Imazeki] the Causal Agent of White Root Rot Disease of Clovesin VitroWhite root rot disease caused by fungi Rigidoporus lignosus (Klotzch) Imazeki is an important disease of cloves that can cause the death of clove plants. Negative effect on the use of intensive fungicides leads to the search of an alternative method which is more environmentally friendly. Oligochitosan is a natural compound that has antifungal activity and can be used as natural pesticide. This study aimed to determine the concentration of oligochitosan that was able to effectively inhibit R. lignosus mycelial growth and to determine the effect of oligochitosan to R. lignosus mycelial growth. The study used a Completely Randomized Design with 5 oligochitosan concentration treatments (2 g/l, 4 g/l, 6 g/l, 8 g/l and 10 g/l) and control (no treatment). Oligochitosan was diluted with water and mixed with PDA to meet the required concentration. Same concentration was also used to dip wooden toothpick for 1 min and incubated on PDA containing R. lignosus. The result showed that 6 g/l oligochitosan concentration was able to inhibit the mycelial growth of R. lignosus up to 71.6%. Highets inhibition of 100% was demonstrated by oligochitosan at concentration of 8 g/l and 10 g/l. The thinning of mycelial growth on the toothpick and microscopic observation demonstrated that the mycelial of R. lignosus were became lysis.Keywords: Antifungal, Food poisonous, Mycelial growth, LysisAbstrakPenyakit Jamur Akar Putih (JAP) yang disebabkan oleh jamur Rigidoporus lignosus (Klotzch) Imazeki merupakan penyakit penting yang menyerang tanaman cengkeh dan bahkan dapat mengakibatkan kematian tanaman. Pengaruh negatif dari penggunaan fungisida mendorong dilakukan pencarian alternatif pengendalian baru yang lebih ramah lingkungan. Oligochitosan merupakan senyawa alami yang mempunyai aktivitas anti jamur dan dapat digunakan sebagai pestisida nabati. Penelitian ini bertujuan untuk mendapatkan konsentrasi oligochitosan yang dapat secara efektif menghambat pertumbuhan jamur R. lignosus dan mengetahui pengaruh oligochitosan terhadap miselia jamur R. lignosus. Percobaan dilakukan dengan menggunakan rancangan acak lengkap dengan perlakuan terdiri dari 5 konsentrasi oligochitosan (2 g/l, 4 g/l, 6 g/l, 8 g/l dan 10 g/l) dan kontrol (tanpa oligochitosan). Oligochitosan dilarutan dalam air dan dicampur dengan PDA sehingga diperoleh konsentrasi yang diuji. Konsentrasi yang sama juga digunakan untuk merendam tusuk gigi selama 1 menit dan diinkubasikan dalam cawan petri yang sebelumnya sudah ditumbuhi oleh R. lignosus. Hasil penelitian menunjukkan bahwa oligochitosan pada konsentrasi 6 g/l menghambat pertumbuhan jamur R. lignosus sebesar 71,6%. Sementara penghambatan tertinggi sebesar 100% diperoleh pada perlakuan oligochitosan dengan konsentrasi8 g/l dan 10 g/l. Penipisan koloni jamur R. lignosus pada tusuk gigi dan pengamatan di bawahmikroskop menunjukkan bahwa oligochitosan menyebabkan lisis pada miselia jamur R. lignosus.Kata kunci: Anti jamur, Umpan beracun, Pertumbuhan koloni, Lisis

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of

In vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups

2000 ◽  
Vol 15 (4) ◽  
pp. 297-308 ◽  
Author(s):  
NAOZUMI TERAMOTO ◽  
YUKIO IMANISHI ◽  
YOSHIHIRO ITO
Keyword(s):  
In Vitro Selection ◽  
Amino Groups ◽  
Selection Of

scholarly journals Back Cover: In Vitro Selection of a DNA Aptamer Targeting Degraded Protein Fragments for Biosensing (Angew. Chem. Int. Ed. 20/2020)

2020 ◽  
Vol 59 (20) ◽  
pp. 7968-7968
Author(s):  
Meng Liu ◽  
Jiayi Wang ◽  
Yangyang Chang ◽  
Qiang Zhang ◽  
Dingran Chang ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Dna Aptamer ◽  
Protein Fragments ◽  
Back Cover ◽  
Selection Of

In vitro selection of a fungal pathogen for use against Diaphorina citri

2015 ◽  
Vol 90 ◽  
pp. 6-15 ◽  
Author(s):  
N. Orduño-Cruz ◽  
A.W. Guzmán-Franco ◽  
E. Rodríguez-Leyva ◽  
R. Alatorre-Rosas ◽  
H. González-Hernández ◽  
...  
Keyword(s):  
Fungal Pathogen ◽  
In Vitro Selection ◽  
Diaphorina Citri ◽  
Selection Of
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