scholarly journals Diversity and activity of cultivated lipophilic bacteria from fat-containing industrial wastes

2020 ◽  
Author(s):  
A. L. Gerasimchuk ◽  
P. A. Bukhtiyarova ◽  
D. V. Antsiferov ◽  
D. A. Ivasenko
Keyword(s):  
Industrial Wastes ◽  
Phylogenetic Groups ◽  
Industrial Wastewaters ◽  
Pure Cultures ◽  
Lipolytic Microorganisms

Pure cultures of lipophilic microorganisms of different phylogenetic groups were isolated from fat-containing industrial wastewaters. The strains of the genera Pseudomonas and Bacillus were the most active lipolytic microorganisms.

scholarly journals New Strategies for Cultivation and Detection of Previously Uncultured Microbes

2004 ◽  
Vol 70 (8) ◽  
pp. 4748-4755 ◽  
Author(s):  
Bradley S. Stevenson ◽  
Stephanie A. Eichorst ◽  
John T. Wertz ◽  
Thomas M. Schmidt ◽  
John A. Breznak
Keyword(s):  
Molecular Techniques ◽  
Integrative Approach ◽  
Growth Media ◽  
Phylogenetic Groups ◽  
Culture Methods ◽  
Acid Analogue ◽  
Pure Cultures ◽  
Agar Media ◽  
Surveillance Method ◽  
New Strategies

ABSTRACT An integrative approach was used to obtain pure cultures of previously uncultivated members of the divisions Acidobacteria and Verrucomicrobia from agricultural soil and from the guts of wood-feeding termites. Some elements of the cultivation procedure included the following: the use of agar media with little or no added nutrients; relatively long periods of incubation (more than 30 days); protection of cells from exogenous peroxides; and inclusion of humic acids or a humic acid analogue (anthraquinone disulfonate) and quorum-signaling compounds (acyl homoserine lactones) in growth media. The bacteria were incubated in the presence of air and in hypoxic (1 to 2% O2 [vol/vol]) and anoxic atmospheres. Some bacteria were incubated with elevated concentrations of CO2 (5% [vol/vol]). Significantly more Acidobacteria were found on isolation plates that had been incubated with 5% CO2. A simple, high-throughput, PCR-based surveillance method (plate wash PCR) was developed. This method greatly facilitated detection and ultimate isolation of target bacteria from as many as 1,000 colonies of nontarget microbes growing on the same agar plates. Results illustrate the power of integrating culture methods with molecular techniques to isolate bacteria from phylogenetic groups underrepresented in culture.

Membrane filtration of agro-industrial wastewaters and isolation of organic compounds with high added values

2013 ◽  
Vol 69 (1) ◽  
pp. 202-207 ◽  
Author(s):  
Dimitris P. Zagklis ◽  
Christakis A. Paraskeva
Keyword(s):  
Organic Compounds ◽  
Membrane Filtration ◽  
Pure Water ◽  
Membrane System ◽  
Industrial Wastes ◽  
Added Value ◽  
Phenolic Fraction ◽  
Industrial Wastewaters ◽  
Organic Fractions ◽  
By Products

The aim of the current study was the exploitation of agro-industrial wastes or by-products such as olive mill wastewater (OMW) and defective wines. A cost-effective system for their maximum exploitation is suggested, using a combined process of membrane filtration and other physicochemical processes. Wastewaters are first treated in a membrane system (prefiltration, ultrafiltration, nanofiltration, and reverse osmosis) where pure water and other organic fractions (by-products) are obtained. Organic fractions, called hereafter byproducts and not wastes, are further treated for the separation of organic compounds and isolation of high added value products. Experiments were performed with OMW and defective wines as characteristic agro-industrial wastewaters. Profit from the exploitation of agro-industrial wastewaters can readily help the depreciation of the indeed high cost process of membrane filtration. The simple phenolic fraction of the OMW was successfully isolated from the rest of the waste, and problems occurring during winemaking, such as high volatile acidity and odours, were tackled.

scholarly journals Effects of Growth Medium, Inoculum Size, and Incubation Time on Culturability and Isolation of Soil Bacteria

2005 ◽  
Vol 71 (2) ◽  
pp. 826-834 ◽  
Author(s):  
Kathryn E. R. Davis ◽  
Shayne J. Joseph ◽  
Peter H. Janssen
Keyword(s):  
New Media ◽  
Growth Medium ◽  
Incubation Time ◽  
Low Frequency ◽  
Viable Count ◽  
Inoculum Size ◽  
Bacterial Cells ◽  
Phylogenetic Groups ◽  
Solid Media ◽  
Pure Cultures

ABSTRACT Soils are inhabited by many bacteria from phylogenetic groups that are poorly studied because representatives are rarely isolated in cultivation studies. Part of the reason for the failure to cultivate these bacteria is the low frequency with which bacterial cells in soil form visible colonies when inoculated onto standard microbiological media, resulting in low viable counts. We investigated the effects of three factors on viable counts, assessed as numbers of CFU on solid media, and on the phylogenetic groups to which the isolated colony-forming bacteria belong. These factors were inoculum size, growth medium, and incubation time. Decreasing the inoculum size resulted in significant increases in the viable count but did not appear to affect colony formation by members of rarely isolated groups. Some media that are traditionally used for soil microbiological studies returned low viable counts and did not result in the isolation of members of rarely isolated groups. Newly developed media, in contrast, resulted in high viable counts and in the isolation of many members of rarely isolated groups, regardless of the inoculum size. Increased incubation times of up to 3 months allowed the development of visible colonies of members of rarely isolated groups in conjunction with the use of appropriate media. Once isolated, pure cultures of members of rarely isolated groups took longer to form visible colonies than did members of commonly isolated groups. Using these new media and extended incubation times, we were able to isolate many members of the phyla Acidobacteria (subdivisions 1, 2, 3, and 4), Gemmatimonadetes, Chloroflexi, and Planctomycetes (including representatives of the previously uncultured WPS-1 lineage) as well as members of the subclasses Rubrobacteridae and Acidimicrobidae of the phylum Actinobacteria.

scholarly journals Search for new cultured lipophilic bacteria in industrial fat-containing wastes

2020 ◽  
Vol 23 ◽  
pp. 02012
Author(s):  
Anna L. Gerasimchuk ◽  
Denis A. Ivasenko ◽  
Polina A. Bukhtiyarova ◽  
Dmitry V. Antsiferov ◽  
Yulia A. Frank
Keyword(s):  
Environmental Problem ◽  
Lipolytic Activity ◽  
Sewage Treatment ◽  
Pathogenic Microorganisms ◽  
Biological Product ◽  
Phylogenetic Groups ◽  
Sewage Treatment Plants ◽  
Pure Cultures ◽  
Brevibacillus Brevis ◽  
Fat Destruction

Fat-containing wastes that are generated as a result of industrial production of food products and are being accumulated in large quantities in wastewater and sewage treatment plants and present a serious environmental problem. Microorganisms that decompose various types of lipids may be potential candidates for creation of commercial bioformulations for fat destruction. The aim of the study was to obtain pure cultures of lipophilic bacteria from fat-containing wastes, to study their diversity and activity for the development of a biological product. As a result, 30 strains of different phylogenetic groups with lipolytic activity was obtained. The most isolated strains were represented by enterobacteria and pseudomonas members within the Gammaproteobacteria. Almost half of the isolated strains were closely related to conditionally pathogenic microorganisms such as Serratia, Klebsiella etc. Non-pathogenic strains and promising for biotechnology ones belonged to Pseudomonas citronellolis, P. nitroreducens, P. synxantha, P. extremaustralis, Bacillus subtilis, B. amyloliquefaciens, Brevibacillus brevis and Microvirgula sp.

Zoosporic fungi from Australian and New Zealand tree-canopy detritus

2005 ◽  
Vol 53 (3) ◽  
pp. 259 ◽  
Author(s):  
Joyce E. Longcore
Keyword(s):  
New Zealand ◽  
Developmental Stages ◽  
Tree Canopy ◽  
Phylogenetic Groups ◽  
Organic Detritus ◽  
The World ◽  
Zoosporic Fungi ◽  
Added Water ◽  
Pure Cultures

The canopy of rainforest trees is an example of a species-rich habitat; however, most of the species known are invertebrates. Zoosporic fungi live in water and soils throughout the world, but have not been reported from the organic detritus of the rainforest canopy. I added water and baits to samples of Australian and New Zealand canopy detritus, and recovered zoosporic fungi representing three major phylogenetic groups, the Chytridiomycota, Oomycota and Hyphochytriomycota. Queensland samples yielded seven chytrid species, a hyphochytrid species and two members of the Saprolegniales. New Zealand samples yielded five chytrids and one member of the Saprolegniales. Most of these fungi were species that have been reported from terrestrial soils or that belong to typically soil-inhabiting groups. Most of the chytrids were isolated into pure cultures and illustrations of their developmental stages are included.

scholarly journals Plasmid pAMI2 of Paracoccus aminophilus JCM 7686 Carries N,N-Dimethylformamide Degradation-Related Genes Whose Expression Is Activated by a LuxR Family Regulator

2010 ◽  
Vol 76 (6) ◽  
pp. 1861-1869 ◽  
Author(s):  
Lukasz Dziewit ◽  
Michal Dmowski ◽  
Jadwiga Baj ◽  
Dariusz Bartosik
Keyword(s):  
Genetic Information ◽  
Sole Source ◽  
Industrial Wastes ◽  
Agrobacterium Radiobacter ◽  
Transcription Regulator ◽  
Industrial Wastewaters ◽  
Transcription Regulators ◽  
Methylotrophic Growth ◽  
High Concentrations ◽  
Sequence Similarities

ABSTRACT N,N-Dimethylformamide (DMF), a toxic solvent used in the chemical industry, is frequently present in industrial wastes. Plasmid pAMI2 (18.6 kb) of Paracoccus aminophilus JCM 7686 carries genetic information which is crucial for methylotrophic growth of this bacterium, using DMF as the sole source of carbon and energy. Besides a conserved backbone related to pAgK84 of Agrobacterium radiobacter K84, pAMI2 carries a three-gene cluster coding for the protein DmfR, which has sequence similarities to members of the LuxR family of transcription regulators, and two subunits (DmfA1 and DmfA2) of N,N-dimethylformamidase, an enzyme of high substrate specificity that catalyzes the first step in the degradation of DMF. Genetic analysis revealed that these genes, which are all placed in the same orientation, constitute an inducible operon whose expression is activated in the presence of DMF by the positive transcription regulator DmfR. This operon was used to construct a strain able to degrade DMF at high concentrations that might be used in the biotreatment of DMF-containing industrial wastewaters. To our knowledge, this is the first study to provide insights into the genetic organization and regulation as well as the dissemination in bacteria of genes involved in the enzymatic breakdown of DMF.

Ferrate vs Traditional Coagulants in the Treatment of Combined Industrial Wastes

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2077-2080 ◽  
Author(s):  
S. J. de Luca ◽  
M. Cantelli ◽  
M. A. de Luca
Keyword(s):  
Pilot Plant ◽  
Industrial Effluents ◽  
Pollutant Removal ◽  
Industrial Wastes ◽  
Regulatory Agencies ◽  
Potassium Ferrate ◽  
Aluminium Sulphate ◽  
Physicochemical Treatment ◽  
Industrial Wastewaters ◽  
Joint Treatment

This study assesses the use of potassium ferrate in the joint treatment of combined domestic and industrial wastewaters. The main feature of this compound is its simultaneous oxidant-coagulant properties. A pilot plant for physicochemical treatment was utilized for tests with combined effluent. The removal of pollutants achieved at the pilot plant fulfills the standards of emission for liquid industrial effluents, established by regulatory agencies. A comparison was also made of the efficiency of pollutant removal between potassium ferrate, ferric chloride and aluminium sulphate for the above-mentioned combined wastewater. All coagulants are very effective in this physicochemical treatment, with an advantage for disinfection, smaller dose and sludge generation to ferrate treatment.

Use of peat in water pollution control: a review

1987 ◽  
Vol 14 (2) ◽  
pp. 230-233 ◽  
Author(s):  
T. Viraraghavan ◽  
A. Ayyaswami
Keyword(s):  
Water Pollution ◽  
Wastewater Treatment ◽  
Key Words ◽  
Pollution Control ◽  
Effective Medium ◽  
Industrial Wastes ◽  
Water Pollution Control ◽  
Industrial Wastewaters ◽  
Onsite Wastewater ◽  
Wastewater Systems

Peat, besides being plentiful and inexpensive, possesses several other qualities that make it an effective medium for the removal of pollutants. This paper presents a review of its use in water pollution control — in the treatment of sanitary and industrial wastewaters. Key words: peat, water pollution control, wastewater treatment, onsite wastewater systems, industrial wastes.

Preliminary results of the optimization of biogas production at the biogas station of the national research institute of animal production in Grodziec Sląski – Kostkowice

2015 ◽  
Vol 2 (3) ◽  
pp. 26-31
Author(s):  
K. Węglarzy ◽  
Yu. Shliva ◽  
B. Matros ◽  
G. Sych
Keyword(s):  
Agricultural Production ◽  
Crude Protein ◽  
Biogas Production ◽  
Electric Energy ◽  
Corn Silage ◽  
Industrial Wastes ◽  
Organic Mass ◽  
National Research Institute ◽  
Digestion Process ◽  
By Products

Aim. To optimize the methane digestion process while using different recipes of substrate components of ag- ricultural origin. Methods. The chemical composition of separate components of the substrate of agricultural by-products, industrial wastes, fats of the agrorefi nery and corn silage was studied. Dry (organic) mass, crude protein (fat) fi ber, loose ash, nitrogen-free exhaust were estimated in the components and the productivity of biogas was determined along with the methane content. These data were used as a basis for daily recipes of the substrate and the analysis of biogas production at the biogas station in Kostkowice. Results. The application of by-products of agricultural production solves the problem of their storage on boards and in open containers, which reduces investment costs, related to the installation of units for their storage. Conclusions. The return on investment for obtaining electric energy out of agricultural biogas depends considerably on the kind of the substrate used and on technological and market conditions.

Review on Analysis of Stir Cast Aluminium Metal Matrix Composite from Agro-Industrial Wastes

2017 ◽  
Vol 5 (2) ◽  
pp. 35 ◽  
Author(s):  
CHANDLA NAGENDER KUMAR ◽  
YASHPAL ◽  
JAWALKAR C. S. ◽  
SURI N. M ◽  
◽  
...  
Keyword(s):  
Metal Matrix Composite ◽  
Matrix Composite ◽  
Metal Matrix ◽  
Industrial Wastes ◽  
Cast Aluminium ◽  
Aluminium Metal
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