Search for new cultured lipophilic bacteria in industrial fat-containing wastes

Fat-containing wastes that are generated as a result of industrial production of food products and are being accumulated in large quantities in wastewater and sewage treatment plants and present a serious environmental problem. Microorganisms that decompose various types of lipids may be potential candidates for creation of commercial bioformulations for fat destruction. The aim of the study was to obtain pure cultures of lipophilic bacteria from fat-containing wastes, to study their diversity and activity for the development of a biological product. As a result, 30 strains of different phylogenetic groups with lipolytic activity was obtained. The most isolated strains were represented by enterobacteria and pseudomonas members within the Gammaproteobacteria. Almost half of the isolated strains were closely related to conditionally pathogenic microorganisms such as Serratia, Klebsiella etc. Non-pathogenic strains and promising for biotechnology ones belonged to Pseudomonas citronellolis, P. nitroreducens, P. synxantha, P. extremaustralis, Bacillus subtilis, B. amyloliquefaciens, Brevibacillus brevis and Microvirgula sp.

Immunohistochemical distribution of irisin in the lungand tongue of Porcupine Hystrix cristata

Irisin, a novel peptide, was first identified in skeletal muscle cells. It is an exercise protein that is secreted into the bloodstream, through which it reached the white adipose tissue, and interacted with an unknown receptor and cause fat destruction by converting the white adipose tissue to brown adipose tissue. The presence of irisin immunoreactivity in porcupine lung and tongue was studied. In lung, irisin immunoreactivity was found in type II pneumocytes, bronchus, bronchioles and blood vessel. Positive irisin immunostaining was also detected in the lamina epithelialis, striated muscle cells and serous glands in porcupine’s tongue. The function of locally synthesized irisin is currently unknown. Therefore, further study is required to reveal the precise effect of irisin.