Occurence of y-hemolysin and panton valentine leukocidin genes and antimicrobial susceptibility patterns of staphylococcus aureus isolated from clinical samples in Lebanon. (c2007)

2007 ◽  
Author(s):  
Pamela F. Abou Khalil
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Clinical Samples ◽  
Panton Valentine Leukocidin ◽  
Susceptibility Patterns ◽  
Valentine Leukocidin

scholarly journals Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city

2018 ◽  
Vol 12 (09) ◽  
pp. 720-725
Author(s):  
Muhammad Shaheen Iqbal ◽  
Yasar Saleem ◽  
Farheen Ansari ◽  
Muhammad Usman Qamar ◽  
Sania Mazhar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Meca Gene ◽  
Toxin Gene ◽  
Mannitol Salt Agar ◽  
Burn Wounds ◽  
High Level ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Introduction: Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates. Methodology: This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients’ departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR. Results: We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively. Conclusions: A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.

scholarly journals SCCmec typing and Panton-valentine leukocidin occurrence in methicillin resistant Staphylococcus aureus (MRSA) isolates from clinical samples of Ahvaz,southwest of Iran

2019 ◽  
Vol 34 (2) ◽  
Author(s):  
Nikou Bahrami ◽  
Hossein Motamedi ◽  
Seyyedeh Elham Reza Tofighi ◽  
Mohammad Reza Akhoond
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Methicillin Resistant ◽  
Sccmec Typing ◽  
Antibiotic Susceptibility Test ◽  
High Prevalence ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Resistance to methicillin in methicillin resistant Staphylococcus aureus (MRSA) is dependent on mecA gene located on staphylococcal cassette chromosome (SCC). Both SCCmec type and Panton-Valentine leukocidin (PVL) affect S. aureus pathogenicity. Aim of this study was to investigate the prevalence of SCCmecA types and pvl genes among MRSA isolates from inpatients. During this cross-sectional study on 100 clinical isolates, following antibiotic susceptibility test, screening of mecA and pvl genes, as well as SCCmec typing, was done in a multiplex PCR technique. From the studied samples, 58 isolates were recognized as MRSA. The frequency of mecA and pvl was 58% and 4%, respectively. All of the MRSA were resistant to cefoxitin and had the highest sensitivity to chloramphenicol. The majority (77.5%) of MRSA was originated from wound samples. The SCCmec III was the most frequent type (22.4%) in these samples. The pvl positive isolates were from SCCmec IVb and V, thus meaning they are from CA-MRSA. These results show a high prevalence of MRSA in the studied region and a widespread prevalence of SCCmec I-V types. Furthermore, high prevalence of SCCmec III indicates the prevalence of multidrug resistant MRSA. This finding is a serious alarm for medical health care practitioners for the correct use of antibiotics in order to limit the spread of multidrug resistant strains. In addition, with regard to life threatening infections caused by pvl harbouring strains, early diagnosis and treatment of infections caused by these isolates should be mandatory.

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