scholarly journals Staphylococcus aureus carrying lukS/F Panton-Valentine Leukocidin (PVL) toxin genes in hospitals of Lahore city

2018 ◽  
Vol 12 (09) ◽  
pp. 720-725
Author(s):  
Muhammad Shaheen Iqbal ◽  
Yasar Saleem ◽  
Farheen Ansari ◽  
Muhammad Usman Qamar ◽  
Sania Mazhar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Tertiary Care ◽  
Meca Gene ◽  
Toxin Gene ◽  
Mannitol Salt Agar ◽  
Burn Wounds ◽  
High Level ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Introduction: Panton Valentine-Leukocidin (PVL) toxin is secreted by Staphylococcus aureus and is mostly associated with skin and soft tissue infections (SSTI). This study aims to find out the prevalence of lukS/F-PV gene, which encode PVL toxin from strains of SSTI, burn wounds and nasal colonizers of out-patients and to measure the antimicrobial susceptibility of S. aureus isolates. Methodology: This is an analytical observational cross-section study and was conducted from July 2014 to June 2015 at four tertiary care hospitals and PCSIR Laboratories Complex, Lahore, Pakistan. A total of 376 random clinical swabs were collected from SSTI (n = 179), nasal nares (n = 134) and burn wounds (n = 63) from out-patients’ departments (OPD). The specimens were cultured on nutrient and mannitol salt agar (MSA) and the organism was identified by catalase, coagulase, and DNase tests. Antimicrobial susceptibility, methicillin, inducible clindamycin, and high-level mupirocin (HLMR) resistance were determined as per CLSI guidelines. Molecular identification of mecA and lukS/F-PV genes was performed by PCR. Results: We isolated 127 S. aureus, where 41 (32.3%) were MRSA and 86 (67.7%) were MSSA. All MRSA carried mecA gene whereas lukS/F-PV gene was found in 21 MRSA and 31 MSSA strains. Overall, a high antimicrobial resistance was found against MRSA and lukS/F-PV positive MSSA. Inducible clindamycin and high-level mupirocin resistance (HLMR) was 23.6% and 19.5% respectively. Conclusions: A high rate of PVL toxin gene was detected among S. aureus strains and a high prevalence of antimicrobial resistant strains was observed.

Panton-Valentine Leukocidin Gene Detected in a Staphylococcus Aureus Strain Isolated from a Knee Arthroprosthesis Infection

2009 ◽  
Vol 32 (9) ◽  
pp. 630-634 ◽  
Author(s):  
Lucio Montanaro ◽  
Lucilla Baldassarri ◽  
Tolmino Corazzari ◽  
Roberta Creti ◽  
Stefano Ravaioli ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Research Unit ◽  
Aureus Strain ◽  
Toxin Gene ◽  
Strain Collection ◽  
Rizzoli Orthopaedic Institute ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

This report focuses on the molecular characterization of a Staphylococcus aureus strain isolated from a knee arthroprosthesis infection and recognized retrospectively as a carrier of the Panton-Valentine leukocidin gene. The stored microbiological isolate, which belonged to the strain collection of the Research Unit on Implant Infections of the Rizzoli Orthopaedic Institute, was retrieved for molecular analysis. Genotyping was carried out, revealing an interesting profile. In addition to the positivity for the Panton-Valentine toxin gene, the results indicated that the isolate belonged to the agr III group and was endowed with bbp and cna genes, both encoding for staphylococcal adhesins that bind bone proteins. The strain had the mecA gene for methicillin resistance, even though it was unable to resist any of the β-lactam or other antibiotics. Its gene configuration matched that of other community-acquired methicillin-resistant and methicillin-susceptible Staphylococcus aureus (CA-MRSA and CA-MSSA) strains which have recently been reported worldwide. As far as we know, this is the first report on a PVL-positive S. aureus strain associated with an orthopedic implant (knee arthroprosthesis) infection.

scholarly journals Prevalence of Panton-Valentine leukocidin genes in community-associated methicillin-resistant Staphylococcus aureus in the district of Pomoravlje

2016 ◽  
Vol 73 (3) ◽  
pp. 256-260 ◽  
Author(s):  
Ljiljana Petrovic-Jeremic ◽  
Nada Kuljic-Kapulica ◽  
Elizabeta Ristanovic ◽  
Dragana Josic ◽  
Zorica Lepsanovic
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Clinical Laboratory ◽  
Meca Gene ◽  
Latex Agglutination ◽  
The Other ◽  
Methicillin Resistant ◽  
Standard Polymerase Chain Reaction ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Background/Aim. Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains appear to have rapidly disseminated among population in the community without established risk factors for MRSA worldwide. Panton-Valentine leukocidin (PVL) is a cytolytic toxin, encoded by the lukF-PV and lukF-PV genes. PVL may be the key toxin responsible for enhanced virulence of CA-MRSA. The aim of this study was to detect the genes encoding PVL in CA-MRSA isolates from healthy people from the District of Pomoravlje. Methods. We took throat and nose swabs from healthy, employed persons with mandatory sanitary examinations and analyzed the presence of MRSA, between January 2011 and December 2012 in the District of Pomoravlje. Susceptibility of isolated strains to cefoxitin was investigated by using disc diffusion according to the recommendation of CLSI (Clinical Laboratory Standard Institute), and by E test. The presence of penicillin-binding protein 2a (PBP2a) in Staphylococci was detected using latex agglutination Slidex ?MRSA Detection test. The gold standard, polymerase chain reaction (PCR) assay, was used for detection of mecA gene and PVL gene, and typing of SCCmec region. Results. Our investigation showed that staphylococcal carrier state was present in 2.58% of 52,910 throat and nasal swabs, and in 50 of them (3.67%) MRSA was isolated. Among these MRSA, 2 (4%) isolates were PVL-positive. Conclusion. The prevalence of CAMRSA and the presence of PVL gene among healthy, employed population in the District of Pomoravlje were low. The values obtained in this study show that, our region is not significantly different from the other parts of our country, nor from the other European countries.

scholarly journals The Role of Staphylococcus aureus in Mastitis

2019 ◽  
Vol 36 (3) ◽  
pp. 503-509 ◽  
Author(s):  
Sara Giordana Rimoldi ◽  
Paola Pileri ◽  
Martina Ilaria Mazzocco ◽  
Francesca Romeri ◽  
Giovanna Bestetti ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Median Number ◽  
Clinical Severity ◽  
Toxin Gene ◽  
Lactation Period ◽  
Breast Abscess ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

Background Breastfeeding women are at risk of developing mastitis during the lactation period. Staphylococcus aureus has emerged as the community-acquired pathogen responsible for virulence (methicillin resistance and Panton-Valentine leukocidin toxin producing). Research aim The aim was to compare the microorganisms responsible for mastitis and breast abscesses during breastfeeding. Methods This observational study was conducted with a sample of women ( N = 60) admitted to our hospital between 2016 and 2018. Participants affected by mastitis and breast abscess were studied and cared for by a multidisciplinary working group. A diagnostic breast ultrasound identified the pathology. Results Twenty-six participants (43.3%) were affected by mastitis and 34 (56.7%) by breast abscess. The most common microorganism identified was Staphylococcus aureus ( S. aureus; mastitis, n = 13; abscesses, n = 24). Methicillin resistance was identified in 21 (44.7%) S. aureus strains: 17 (80.9%) cases of abscess and four (19.1%) cases of mastitis. The median number of months of breastfeeding was smaller in the methicillin-resistant S. aureus (MRSA) cases (median = 3, range = 1–20 months) than in the methicillin-sensitive S. aureus (MSSA) cases (median = 6.5, range = 3–21 months). The Panton-Valentine leukocidin toxin gene was detected in 12 (25.5%) cases (MRSA, n = 8, 66.7%; MSSA, n = 4, 33.3%). Hospitalization was required more frequently in MRSA ( n = 8, 38%; five Panton-Valentine leukocidin positive) than in MSSA cases ( n = 5, 19%; one Panton-Valentine leukocidin positive). Four women out of the eight MRSA cases (50%) that were Panton-Valentine leukocidin positive stopped breastfeeding during mammary pathologies, three (37.5%) participants continued breastfeeding until the follow-up recall, and one case was lost at follow-up. Conclusion Clinical severity was probably complicated by the presence of the Panton-Valentine leukocidin toxin, which required hospitalization more frequently.

scholarly journals High Incidence of Panton-Valentine Leukocidin-Producing Staphylococcus aureus in a Tertiary Care Public Hospital in Singapore

2005 ◽  
Vol 40 (3) ◽  
pp. 486-489 ◽  
Author(s):  
H. Li-Yang ◽  
K. Tse-Hsien ◽  
K. Asok ◽  
L. Jenny ◽  
P. C. Maciej ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Public Hospital ◽  
Tertiary Care ◽  
High Incidence ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin
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