Comparison of Staphylococcal cassette chromosome mectypes and antimicrobial susceptibility patterns of Staphylococcus aureus isolated from clinical samples in Lebanon. (c2007)

2007 ◽  
Author(s):  
Dina Jabbour
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Clinical Samples ◽  
Susceptibility Patterns ◽  
Staphylococcal Cassette Chromosome

scholarly journals The SCCmec Types and Antimicrobial Resistance among Methicillin-Resistant Staphylococcus Species Isolated from Dogs with Superficial Pyoderma

2021 ◽  
Vol 8 (5) ◽  
pp. 85
Author(s):  
Yuttana Chanayat ◽  
Areerath Akatvipat ◽  
Jeff B. Bender ◽  
Veerasak Punyapornwithaya ◽  
Tongkorn Meeyam ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Sccmec Type ◽  
Small Animal ◽  
Control Measures ◽  
Infection Prevention And Control ◽  
Clinical Samples ◽  
Biochemical Tests ◽  
Methicillin Resistant ◽  
Type V ◽  
Staphylococcal Cassette Chromosome

This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.

scholarly journals 467. Antimicrobial Resistance and Molecular Analysis of Staphylococcus aureus in Staphylococcal Scalded Skin Syndrome among Children in Korea

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S229-S229
Author(s):  
Jae Hong Choi ◽  
Hyunju Lee ◽  
Eun Hwa Choi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Molecular Characteristics ◽  
University Hospitals ◽  
Korean Children ◽  
Methicillin Resistant ◽  
Staphylococcal Scalded Skin Syndrome ◽  
High Prevalence ◽  
The Mean ◽  
Susceptibility Patterns

Abstract Background Staphylococcal scalded skin syndrome (SSSS) is a blistering and desquamative skin disease caused by the exfoliative toxins of Staphylococcus aureus. SSSS mainly affects children younger than 5 years of age. Although many countries show a predominance of methicillin-susceptible S. aureus (MSSA), recently an increase in cases due to methicillin-resistant S. aureus (MRSA) has been reported. We investigated the molecular characteristics of S. aureus isolated from the children with SSSS in Korea. Methods From January 2010 to December 2017, children clinically diagnosed as SSSS under the age of 5 years were enrolled. Cases from 3 different university hospitals in Korea were included. S. aureus isolated from nasal, axillary, or inguinal area of the children were analyzed for multilocus sequence type and exfoliative toxins (eta, etb). Medical records were retrospectively reviewed for clinical characteristics and antimicrobial susceptibility patterns of S. aureus. Results A total of 26 cases were enrolled. The mean age was 2.3 years (range, 0–4.8 years). Twenty-two (84.6%) patients were hospitalized. Skin manifestations were classified as follows; generalized (n = 10, 38.5%), intermediate (n = 11, 42.3%), and abortive (n = 5, 19.2%). Twenty-five isolates (96.2%) were resistant to methicillin and macrolide-resistance was found in 92.3% (n = 24). ST89 (n = 21, 80.8%) was the most prevalent clone, with single clones of ST1, ST5, ST72, ST121, and ST1507. The eta gene was detected in 1 (3.8%) MSSA isolate. The etb gene was detected in 14 (53.8%) isolates all of which were ST89. All patients were treated with antibiotics, and the mean duration was 8.3 days regardless of the administration route. Nafcillin or first cephalosporin was most commonly prescribed (n = 20, 76.9%), clindamycin was administered in combination in 9 patients (34.6%) and vancomycin in 4 patients (15.4%). Among the 25 MRSA cases, only 6 (24.0%) were treated with susceptible antibiotics. However, there was no difference in treatment duration according to antimicrobial susceptibility (8.43:8.22 days, P > 0.05). Conclusion The molecular epidemiology of S. aureus isolated from the Korean children with SSSS demonstrated the high prevalence of methicillin-resistant ST89 clone that harbors the etb gene. Disclosures All authors: No reported disclosures.

scholarly journals Antimicrobial susceptibility patterns of β-lactamase producing Staphylococcus Aureus

1970 ◽  
Vol 4 (2) ◽  
pp. 14-17
Author(s):  
Narjis Maliha Kawsar ◽  
Md Eunus Ali Mondal ◽  
Nizamul Karim Khan ◽  
Nishat Jubaida ◽  
Jamal Pasha Chowdhury ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Patterns

doi:10.3329/jafmc.v4i2.1838JAFMC Bangladesh Vol.4(2) 2008 14-17

scholarly journals Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Salu Rai ◽  
Uday Narayan Yadav ◽  
Narayan Dutt Pant ◽  
Jaya Krishna Yakha ◽  
Prem Prasad Tripathi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Gram Negative Bacteria ◽  
Wound Infections ◽  
Gram Negative ◽  
Methicillin Resistant ◽  
Gram Positive Bacteria ◽  
Wound Swab ◽  
Susceptibility Patterns

In Nepal, little is known about the microbiological profile of wound infections in children and their antimicrobial susceptibility patterns. Total of 450 pus/wound swab samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by Kirby-Bauer disc diffusion technique. Methicillin-resistantStaphylococcus aureusisolates were detected by using cefoxitin disc and confirmed by determining minimum inhibitory concentrations (MIC) of oxacillin. 264 (59%) samples were culture positive. The highest incidence of bacterial infections was noted in the age group of less than 1 year (76%). Out of 264 growth positive samples, Gram-positive bacteria were isolated from 162 (61%) samples and Gram-negative bacteria were found in 102 (39%) samples.Staphylococcus aureus(99%) was the predominant Gram-positive bacteria isolated andPseudomonas aeruginosa(44%) was predominant Gram-negative bacteria. About 19% ofS. aureusisolates were found to be methicillin-resistant MIC of oxacillin ranging from 4 μg/mL to 128 μg/mL. Among the children of Nepal, those of age less than 1 year were at higher risk of wound infections by bacteria.Staphylococcus aureusfollowed byPseudomonas aeruginosawere the most common bacteria causing wound infections in children.

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