scholarly journals Dietary forage to concentrate ratio and sunflower oil supplement alter rumen fermentation, ruminal methane emissions, and nutrient utilization in lactating cows1

2017 ◽  
Vol 1 (3) ◽  
pp. 277-286 ◽  
Author(s):  
A. R. Bayat ◽  
L. Ventto ◽  
P. Kairenius ◽  
T. Stefański ◽  
H. Leskinen ◽  
...  
Keyword(s):  
Sunflower Oil ◽  
Latin Square ◽  
Nutrient Utilization ◽  
Rumen Fermentation ◽  
Fiber Digestion ◽  
Ch4 Emissions ◽  
Grass Silage ◽  
Lactating Cows ◽  
Rumen Ph ◽  
Experimental Treatments

Abstract The effects of supplementing high- or low-concentrate diets with sunflower oil (SO) on rumen fermentation, nutrient utilization, and ruminal methane (CH4) emissions in lactating cows were examined. Four multiparous Nordic Red dairy cows fitted with rumen cannulae were used in a 4 × 4 Latin square with a 2 × 2 factorial arrangement of treatments and 35-d periods. Experimental treatments comprised iso-nitrogenous total mixed rations based on grass silage with forage to concentrate ratio of 65:35 or 35:65 supplemented with 0 or 50 g/kg diet DM of SO. Apparent ruminal OM and starch digestibility was greater (P < 0.05) with high- than low-concentrate diets but was unaffected by SO. Inclusion of SO in high-concentrate diet decreased (P ≤ 0.05) apparent total tract OM, fiber, and GE, and apparent ruminal fiber digestibility. High-concentrate diets and SO shifted (P < 0.05) fiber digestion from rumen to the hindgut. High-concentrate diet resulted in a lower rumen pH and elevated total rumen VFA concentration compared with low-concentrate diet, whereas SO increased rumen pH and decreased rumen VFA concentration when included in high-, but not low-concentrate diet (P < 0.05 for interaction). High-concentrate diet reduced rumen ammonia-N (P < 0.01) and molar proportion of acetate to propionate (P < 0.01), and decreased (P < 0.05) ruminal CH4 emissions when expressed as g/d or g/kg OM digested in the rumen. With both low- and high-concentrate diets, SO reduced (P < 0.05) daily emissions of CH4 as g/d or g/kg OM digested in the rumen, but SO reduced CH4 emissions expressed as g/kg OM intake, OM digested in total digestive tract, energy-corrected milk or % of GE intake only with low-concentrate diet (P ≤ 0.05 for interaction). In conclusion, replacing grass silage with concentrates led to a reduction in daily ruminal CH4 emissions that were accompanied by a shift in rumen fermentation toward the synthesis of propionate, and decreases in rumen pH and fiber digestion. Sunflower oil was effective in reducing daily CH4 emissions in lactating cows which was accompanied by a noticeable lower feed intake with high- but not low-concentrate diet. Overall the effects of SO and greater proportion of concentrates in the diet on daily CH4 emissions were additive but the additivity declined or vanished when different indices of CH4 emission intensity were considered. Consequently, SO was more effective in reducing CH4 emissions when low-concentrate diet was fed.

scholarly journals Effects of Starch Level and a Mixture of Sunflower and Fish Oils on Nutrient Intake and Digestibility, Rumen Fermentation, and Ruminal Methane Emissions in Dairy Cows

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1310
Author(s):  
Babak Darabighane ◽  
Ilma Tapio ◽  
Laura Ventto ◽  
Piia Kairenius ◽  
Tomasz Stefański ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Fat ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Fish Oils ◽  
Ch4 Emissions ◽  
Gross Energy ◽  
Experimental Treatments ◽  
Dietary Treatments ◽  
Dietary Oil

Four multiparous dairy cows were used in a 4 × 4 Latin square to examine how starch level and oil mixture impact dry matter (DM) intake and digestibility, milk yield and composition, rumen fermentation, ruminal methane (CH4) emissions, and microbial diversity. Experimental treatments comprised high (HS) or low (LS) levels of starch containing 0 or 30 g of a mixture of sunflower and fish oils (2:1 w/w) per kg diet DM (LSO and HSO, respectively). Intake of DM did not differ between cows fed LS and HS diets while oil supplementation reduced DM intake. Dietary treatments did not affect milk and energy corrected milk yields. There was a tendency to have a lower milk fat concentration due to HSO compared with other treatments. Both high starch level and oil supplementation increased digestibility of gross energy. Cows receiving HS diets had higher levels of total rumen VFA while acetate was lower than LS without any differences in rumen pH, or ruminal CH4 emissions. Although dietary oil supplementation had no impact on rumen fermentation, decreased CH4 emissions (g/day and g/kg milk) were observed with a concomitant increase in Anoplodinium-Diplodinium sp. and Epidinium sp. but a decrease in Christensenellaceae, Ruminococcus sp., Methanobrevibacter ruminantium and Mbb. gottschalkii clades.

Effect of monensin on in vitro semi-continuous rumen fermentation in the rumen simulation technique (Rusitec)

1996 ◽  
Vol 1996 ◽  
pp. 213-213
Author(s):  
E.D. Mackintosh ◽  
R.H. Phipps ◽  
J.D. Sutton ◽  
J. Wilkinson
Keyword(s):  
Volatile Fatty Acid ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Protein Supplement ◽  
Wheat Grain ◽  
Maize Silage ◽  
Grass Silage ◽  
Complete Diet ◽  
Holstein Friesian

Rusitec (Czerkawski and Breckenndge, 1977) lias been widely used to study factors which affect rumen fermentation such as monensin, monensin-propionate, abierixin, calcimycin and Aspergillus oryzae (Bogaert et al., 1990; Newbold et al., 1993). Monensin is a grain-positive ionophore which modifies rumen fermentation. The objective of the current study was to determine the effect of monensin on molar proportions of volatile fatty acid (VFA) produced from diets which varied in foragexoncentrate ratio.Four Rusitec vessels (800 ml) were used in an extended Latin Square design with 2 blocks, 2 treatments and 3 periods, each lasting 14 days. To initiate each period, inocula was recovered from the same lactating Holstein-Friesian cow (∽650 kg). All vessels received 15 g DM/day of a complete diet which contained maize silage, grass silage, NaOH treated wheat grain and a protein supplement in three foragexoncentrate ratios; 25:75 (L), 50:50 (M) and 75:25 (H).

Apparent digestibility, rumen fermentation and nitrogen balance in Tibetan and fine-wool sheep offered forage-concentrate diets differing in nitrogen concentration

2015 ◽  
Vol 153 (6) ◽  
pp. 1135-1145 ◽  
Author(s):  
J. W. ZHOU ◽  
J. D. MI ◽  
A. A. DEGEN ◽  
X. S. GUO ◽  
H. C. WANG ◽  
...  
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nitrogen Concentration ◽  
Latin Square ◽  
Rumen Fermentation ◽  
N Retention ◽  
Dietary Nitrogen ◽  
Rumen Ph ◽  
Tibetan Sheep ◽  
Acid Detergent Fibre

SUMMARYA comparative study of the effect of dietary nitrogen (N) content [Low: 11·0; Medium-Low (MLow): 16·7; Medium-High (Mhigh): 23·1; High: 29·2 N g/kg dry matter (DM)] on apparent digestibilities, rumen fermentation and N balance was conducted in coarse wool Tibetan sheep and Gansu Alpine fine-wool sheep at Wushaoling in the northeast of the Qinghai-Tibetan Plateau. It was hypothesized that responses would differ between breeds and that responses would favour Tibetan over fine-wool sheep at low N intakes. Eight wethers [four Tibetan sheep and four fine-wool sheep, 20–24 months old; body weight ± standard deviation was 52 ± 3·2 kg] were used in two concurrent 4 × 4 Latin square designs. Dry matter, organic matter, neutral detergent fibre and acid detergent fibre digestibilities were higher in Tibetan than fine-wool sheep when fed the Low, MLow and High N diets while N retention was higher when the animals were fed the Low and MLow N diets. Tibetan sheep had a higher rumen pH than fine-wool sheep; however, total volatile fatty acids were similar between breeds. Molar proportions of acetate were higher but propionate and butyrate lower in Tibetan than fine-wool sheep. In addition, Tibetan sheep had higher concentrations of ruminal free amino acid-N and soluble protein-N than fine-wool sheep. Plasma and saliva urea-N concentrations were higher in Tibetan than fine-wool sheep when supplied with the Low N diet. It was concluded that Tibetan sheep were better able to cope with low N feed than fine-wool sheep because of the higher N retention and higher DM and fibre digestibilities with Low and MLow diets.

scholarly journals Effect of incremental levels of sunflower-seed oil in the diet on ruminal lipid metabolism in lactating cows

2008 ◽  
Vol 99 (5) ◽  
pp. 971-983 ◽  
Author(s):  
K. J. Shingfield ◽  
S. Ahvenjärvi ◽  
V. Toivonen ◽  
A. Vanhatalo ◽  
P. Huhtanen ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Milk Fat ◽  
Sunflower Seed ◽  
Seed Oil ◽  
Latin Square ◽  
Grass Silage ◽  
Sunflower Seed Oil ◽  
Lactating Cows ◽  
Potential Benefits ◽  
Nutritional Strategies

Based on the potential benefits ofcis-9,trans-11-conjugated linoleic acid (CLA) for human health there is interest in developing sustainable nutritional strategies for enhancing the concentration of this fatty acid in ruminant-derived foods. Most evidence to date suggests that endogenous synthesis is the major source ofcis-9,trans-11 in milk fat and ruminal outflow is limited and largely independent of dietary 18 : 2n-6 supply. Four lactating cows fitted with a rumen cannula were used in a 4 × 4 Latin square with 14 d experimental periods to examine the effects of sunflower-seed oil (SFO) as a source of 18 : 2n-6 on ruminal lipid metabolism. Cows were offered grass silage-based diets supplemented with 0, 250, 500 or 750 g SFO/d. Supplements of SFO had no effect on DM intake, milk fat or protein secretion, but increased linearly (P < 0·01) milk yield and milk lactose output and shifted (P < 0·001) rumen fermentation towards propionate at the expense of acetate. SFO supplements increased linearly (P < 0·05) the flow of 18 : 0, 18 : 1, 18 : 2n-6 and total CLA at the omasum and enhanced ruminalcis-9-18 : 1, 18 : 2n-6 and 18 : 3n-3 metabolism. Flows of all-trans- (Δ4–16) andcis- (Δ9–16) 18 : 1 isomers were elevated, while increases in ruminal CLA outflow were confined totrans-8,trans-10 and geometric 9,11 and 10,12 isomers. It is concluded that supplementing grass silage-based diets with plant oils rich in 18 : 2n-6 enhances ruminal outflow oftrans-11-18 : 1 andcis-9,trans-11-CLA in lactating cows.

Rice polishings as an alternative to sugar cane molasses as a supplement with urea to low-quality forage diets for ruminants

1993 ◽  
Vol 56 (1) ◽  
pp. 85-92 ◽  
Author(s):  
D. Cardenas Garcia ◽  
C. J. Newbold ◽  
H. Galbraith ◽  
J. H. Topps ◽  
X. B. Chen ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Latin Square ◽  
Experimental Period ◽  
Rumen Fermentation ◽  
Cane Molasses ◽  
Mean Values ◽  
Rumen Ph ◽  
Protein Supply

AbstractThe effect of including 14 g urea with either 75 g dry matter (DM) from sugar cane molasses (UM) or Colombian rice polishings (RP) at three levels, 68 (RP1), 137 (RP2) or 203 (RP3) g DM on grass hay DM intake and on rumen fermentation was investigated. An incomplete Latin-square design was used and each experimental period was divided into 12 days for adaptation to each diet followed by 9 days in metabolism cages when all measurements were made using five adult sheep.Pooled mean values for rumen metabolites (five samples per day) were calculated. Rumen pH was not affected by the nature of the supplements. Concentrations of volatile fatty acids (VFA) (UM 92·8, RP1 84·2, RP2 86·4, and RP3 84·0 (s.e.d. 3·4) mmol/l) and lactate (UM 2·0, RP1 1/6, RP2 1/7, RP3 1/8 (s.e.d. 0·014) mmol/l) (UM v. RP, P < 0·05 and P < 0·10 respectively) were lower when RP were given, while concentration of branched and longer chain VFA (26·5, 34·0, 31·1 and 33·5 (s.e.d. 1·6) mmol/mol total VFA, UM v. RP, P < 0·01) and ammonia (98, 131, 141, 137 (s.e.d. 16·1) mg/l, UM v. RP, P < 0·05) were increased. Numbers of rumen protozoa (1·6, 3·2, 2·7, 3·3 (s.e.d. 0·75) × 105 per ml, UM v. RP, P < 0·20) tended to be higher 2 h after feeding when RP rather than UM were given. However, hay DM intake (1050, 960, 960, 880 (s.e.d. 45·3) g/day, UM v. RP, P < 0·05), and microbial protein supply (11·7, 9·3,11·1,10·8 (s.e.d. 0·59) g N per day, UM v. RP, P < 0·05), estimated from urinary purine excretion were reduced by feeding RP instead of UM.At the levels of inclusion tested RP did not increase the efficiency of rumen fermentation and were not as effective a supplement with urea as was molasses for a low-quality forage diet.

scholarly journals Milk fatty acid composition in Holstein x Gyr dairy cows fed chopped elephantgrass-based diets containing two types of sunflower oil associated with two methods of concentrate feeding

2020 ◽  
Vol 41 (6) ◽  
pp. 2759-2778
Author(s):  
Fernando César Ferraz Lopes ◽  
◽  
Carlos Gustavo Santos Ribeiro ◽  
Norberto Mario Rodriguez ◽  
Marco Antônio Sundfeld da Gama ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Palmitic Acid ◽  
Sunflower Oil ◽  
Milk Fat ◽  
Nutrient Intake ◽  
Block Design ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
High Oleic

wo experiments were carried out in a 2 x 2 factorial arrangement with the objective of evaluating two methods of concentrate feeding for Holstein x Gyr cows fed 600 g kg-1 chopped elephantgrass-based diets supplemented at 45 g kg-1 DM with two types of sunflower oil (SO). The types of SO differed in the levels of oleic and linoleic fatty acids (FAs): high oleic/low linoleic acid – HO (73 and 10 g 100 g-1 FA, respectively) and medium oleic/medium linoleic acid – MO (43 and 34 g 100 g-1 FA, respectively). The concentrates containing HO SO or MO SO were supplied separately from the forage twice a day after the two milkings (TAD) or as part of a total mixed ration (TMR). In Experiment 1, a 4 x 4 Latin square design was used to evaluate the ruminal fermentation and degradation parameters in four rumen-cannulated cows (430±39 kg; 79±20 days in milk; 16.4±3.1 kg day-1 of milk). In Experiment 2, a randomized block design was used to evaluate the nutrient intake, plasma contents of metabolites and FAs, milk yield and composition, and FA profile of milk fat in 32 cows (444±84 kg; 75±31 days in milk; 15.4±4.8 kg day-1 of milk). The results were analyzed by mixed models (P ? 0.05). The TMR diets promoted higher nutrient intake and rumen fermentation (higher ammonia N, acetate, propionate and total volatile FA contents) without affecting milk, fat, protein and lactose yields. TAD-fed cows presented higher feed efficiency and produced milk fat with a more nutritionally desirable FA composition, with higher vaccenic and rumenic acid contents and lower trans-10 C18:1 and palmitic acid contents. The DM intake, parameters of rumen fermentation and milk, fat, protein and lactose yields were similar for the HO SO and MO SO diets. The most nutritionally positive characteristics for human health in the milk fat of HO SO-fed cows were the higher eicosapentaenoic (+34%) and oleic acid (+11%) contents and lower palmitic acid content (-10%). Higher contents of vaccenic (+71%) and rumenic (+74%) acids and lower trans-10 C18:1 (-10%), elaidic (-32%), lauric (-14%) and myristic (-11%) acid contents were the most positive aspects of the milk fat of MO SO-fed cows. Considering the magnitudes of the differences in the levels of these FAs, it is concluded that the milk fat of cows fed MO SO showed a healthier milk FA profile.

scholarly journals Autumn-cut grass silage as roughage component in dairy cow rations. 2. Rumen degradation, fermentation and kinetics

1993 ◽  
Vol 41 (3) ◽  
pp. 221-234
Author(s):  
H. De Visser ◽  
H. Huisert ◽  
A. Klop ◽  
R.S. Ketelaar
Keyword(s):  
Rumen Fluid ◽  
Latin Square ◽  
Basal Diet ◽  
Rumen Fermentation ◽  
High Moisture ◽  
Rumen Degradation ◽  
Grass Silage ◽  
Branched Chain ◽  
Gluten Feed

In a 4 x 4 Latin square experiment the effects of DM content and/or the extent of fermentation in grass silages on the pattern of rumen fermentation and rumen kinetics were studied. In a separate study two cows were used to measure the rate of degradation using the dacron bag technique. Four rumen cannulated dairy cows were used to measure rumen fermentation pattern, rumen kinetics were measured in three of these animals. Basal diets (70% of total DM) consisted of maize silage, moist ensiled beet pulp, moist ensiled maize gluten feed, moist ensiled brewers' grains and a concentrate mixture. The remainder of the diet (30% of total DM) was wilted grass silage (WGS), high moisture grass silage with molasses (MGS), high moisture silage with formic acid (FGS) or wilted grass silage with additional water (WW). All diets were fed as complete feeds. pH of the rumen fluid was lower on the MGS and FGS diets. Concentrations of total VFA, acetic acid, ammonia and branched-chain fatty acids (BCFA) were highest on high moisture diets (MGS and FGS). Rates of clearance and digestion of the organic matter (OM) fractions were or showed tendencies towards being negatively influenced by both MGS and FGS, but remained unaffected by WGS and WW. Degradability of the grass silages was influenced by fermentation in the silo (lower digestible fractions and higher soluble fractions), as were rates of degradation (higher). Results of the degradability measured on the basal diet ingredients were in agreement with published literature and showed a strong correlation between OM digestibility in vitro and the undigestible fraction.

INFLUENCE ON SOME RUMEN AND BLOOD PARAMETERS OF FEEDING ACETOHYDROXAMIC ACID IN A UREA-CONTAINING RATION FOR LAMBS

1975 ◽  
Vol 55 (1) ◽  
pp. 39-47 ◽  
Author(s):  
G. A. JONES ◽  
J. D. MILLIGAN
Keyword(s):  
Nitrogen Concentration ◽  
Latin Square ◽  
Bacterial Count ◽  
Blood Parameters ◽  
Rumen Fermentation ◽  
Design Experiment ◽  
Acetohydroxamic Acid ◽  
Molar Ratios ◽  
Rumen Ph ◽  
Volatile Fatty Acid Concentration

A 4 × 4 latin square design experiment, involving four fistulated Dorset–Columbia crossbred lambs, was used to determine the effects of feeding the urease inhibitor acetohydroxamic acid (AHA) with a urea-containing ration on some parameters of the rumen fermentation and blood. AHA, administered in the feed at concentrations up to and including 1.5%, markedly depressed rumen urease activity and the peak concentration of rumen ammonia. The maximum AHA concentration achieved in the rumen solution was 32.29 ± 14.26 mg per 100 ml, and thereafter the concentration dropped by 66% during the next 2 h. No evidence was found for an effect of AHA upon rumen pH, viable bacterial count, total protozoal count, total volatile fatty acid concentration, molar ratios of acetate, propionate and n-butyrate, or upon blood urea-nitrogen concentration. Traces of AHA were found in peripheral blood both 1 and 3 h after feeding, showing that AHA is absorbed from the ruminant digestive tract. It was concluded that AHA did not influence major parameters of the rumen fermentation at concentrations that effectively inhibited rumen urease.

scholarly journals 98 Effect of engineered biocarbon on rumen fermentation, nutrient digestibility, methane emissions, and rumen microbiota in beef heifers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 82-83
Author(s):  
Stephanie Terry ◽  
Gabriel Ribeiro ◽  
Robert Gruninger ◽  
Alex Chaves ◽  
Karen Beauchemin ◽  
...  
Keyword(s):  
Fixed Effects ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Barley Grain ◽  
Open Circuit ◽  
Nutrient Digestibility ◽  
Mineral Supplement ◽  
Fecal Microbiome ◽  
Ch4 Emissions ◽  
Barley Silage

Abstract The objective of this study was to examine the effect of engineered biocarbon (EB) on rumen fermentation, apparent total tract digestibility, methane (CH4) emissions and the rumen and fecal microbiome of Angus × Hereford heifers fed a barley silage-based diet. The experiment was a replicated 4 × 4 Latin square using 8 ruminally cannulated heifers (565 ± 35 kg initial BW). The basal diet contained 60% barley silage, 35% barley grain and 5% mineral supplement with EB added at 0% (control), 0.5%, 1.0%, or 2.0% (DM basis). Each period was 28-d consisting of 14-d adaptation and 14-d of measurements. Samples for profiling of the microbiome in rumen liquid, solids and feces were collected on d-15 before feeding. Rumen samples for fermentation characterization were taken at 0, 3, 6, and 12-h post feeding. Total collection of urine and feces was conducted from d-18 to 22. Heifers were housed in open-circuit respiratory chambers on d-26 to 28 to estimate CH4 emissions. Ruminal pH was recorded at 1-min intervals during CH4 measurements using indwelling pH loggers. Data were analyzed with the fixed effects of dietary treatment and random effects of square, heifer within square and period. Dry matter intake was similar across treatments (P = 0.21). NH3-N concentration and protozoa counts responded quadratically (P = 0.01) to EB. Minimum pH was increased (P = 0.04), and variation of pH was decreased (P = 0.03) by 2.0% EB. Total tract digestibility, N balance and CH4 production were not affected (P ≥ 0.17) by EB. EB affected the relative abundance of Fibrobacter (P = 0.05), Spirochaetaes (P = 0.01), Verrucomicrobia (P = 0.02), Tenericutes (P = 0.01), and Elusimicrobia (P = 0.02). Results suggest that at the examined concentrations, EB was ineffective at decreasing enteric CH4 emissions, however it altered the rumen microbiome.

Sign in / Sign up

Export Citation Format

Share Document