scholarly journals The Influences of Growth Regulators and Culture Medium Composition on Shoot-Tip Cultures of Edible Canna

2007 ◽  
Vol 45 (3) ◽  
pp. 155-163 ◽  
Author(s):  
Takanari SAKAI ◽  
Katsu IMAI
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Medium Composition ◽  
Shoot Tip

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

Influences of Growth Regulators and Cultivars on Callus and Shoot Production of Alfalfa

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 461f-462
Author(s):  
Guochen Yang ◽  
Marihelen Kamp-Glass
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Shoot Organogenesis ◽  
Multiple Shoots ◽  
Apparent Difference ◽  
Shoot Production ◽  
Callus Initiation ◽  
Dark Green ◽  
Number Of Shoots ◽  
Alfalfa Seeds

Alfalfa seeds of Cimarron VR, CW1446, CW2440, C94-118, C94-785, and WL311 were used as explants. BA, zeatin, and TDZ were evaluated on callus initiation, development, and shoot production. Callus initiation and development toward shoot organogenesis were enhanced when BA was added in the culture medium. Calli produced from BA treatments were compact, solid, and dark green. Similar results were obtained when zeatin was added in the culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly from each individual explant when TDZ was added to culture medium. However, when higher concentration of TDZ was used, number of shoots per explant was decreased, and dwarf shoots were produced. No apparent difference on shoot production was observed among the cultivars tested so far. Data on number of shoots per explant from two of these cultivars need to be statistically analyzed.

Differentiation of mesenchymal stem cells from humans and animals into insulin-producing cells: An overview in vitro induction forms

2020 ◽  
Vol 16 ◽  
Author(s):  
Bruna O. S. Câmara ◽  
Bruno M. Bertassoli ◽  
Natália M. Ocarino ◽  
Rogéria Serakides
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture Medium ◽  
Adipogenic Differentiation ◽  
Medium Composition ◽  
Cell Therapies ◽  
Insulin Producing Cells ◽  
Msc Differentiation

The use of stem cells in cell therapies has shown promising results in the treatment of several diseases, including diabetes mellitus, in both humans and animals. Mesenchymal stem cells (MSCs) can be isolated from various locations, including bone marrow, adipose tissues, synovia, muscles, dental pulp, umbilical cords, and the placenta. In vitro, by manipulating the composition of the culture medium or transfection, MSCs can differentiate into several cell lineages, including insulin-producing cells (IPCs). Unlike osteogenic, chondrogenic, and adipogenic differentiation, for which the culture medium and time are similar between studies, studies involving the induction of MSC differentiation in IPCs differ greatly. This divergence is usually evident in relation to the differentiation technique used, the composition of the culture medium, the cultivation time, which can vary from a few hours to several months, and the number of steps to complete differentiation. However, although there is no “gold standard” differentiation medium composition, most prominent studies mention the use of nicotinamide, exedin-4, ß-mercaptoethanol, fibroblast growth factor b (FGFb), and glucose in the culture medium to promote the differentiation of MSCs into IPCs. Therefore, the purpose of this review is to investigate the stages of MSC differentiation into IPCs both in vivo and in vitro, as well as address differentiation techniques and molecular actions and mechanisms by which some substances, such as nicotinamide, exedin-4, ßmercaptoethanol, FGFb, and glucose, participate in the differentiation process.

scholarly journals Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

2017 ◽  
Vol 72 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Wagner de Melo Ferreira ◽  
Marcelo Cardoso de Vasconcelos ◽  
Clede Cléia Neves Silva ◽  
Hugo Rivas de Oliveira ◽  
Rogério Mamoru Suzuki
Keyword(s):  
Growth Regulators ◽  
Culture Medium ◽  
Asymbiotic Germination

scholarly journals Efficiency of using different types induction culture medium for hexaploid triticale anther cultivation

2019 ◽  
Vol 25 ◽  
pp. 260-265
Author(s):  
E. V. Lagunovskaya ◽  
O. I. Zaitseva ◽  
V. A. Lemesh
Keyword(s):  
Culture Medium ◽  
Medium Composition ◽  
Induction Medium ◽  
Hexaploid Triticale ◽  
Culture Methods ◽  
Study Results ◽  
Short Period ◽  
Androgenesis In Vitro ◽  
The Republic

Aim. Triticale is one of the main grain crops of the Republic of Belarus. Further progress in the selection of this culture involves the accelerated creation of highly productive early ripening varieties resistant to abiotic and biotic factors. The method of induced androgenesis in vitro makes it possible to obtain stable homozygous lines in a short period of time and to eliminate the lengthy process of inbreeding used in classical breeding to fix the desired traits. Methods. The tissue and cell culture methods for plants was used in the study. Results. The influence of the induction medium composition on the efficiency of in vitro induced androgenesis in varieties and lines of hexaploid triticale is assessed. The influence of three types of induction culture medium, the type of phytohormones and the presence or absence of cefotaxime in the medium are analyzed. Results. It has been shown that using the C-17 culture medium supplemented with 2.0 mg/l 2,4-D and 0.5 mg/l kinetin without adding cefotaxime is most effective for the anther triticale cultivation. Keywords: triticale, anther culture, induction nutrient medium, embryoids, calli, regenerant plants, cefotaxime.

Influence de phénomènes d'antagonisme entre organes et entre régulateurs sur le développement floral de bourgeons cotylédonaires chez le Scrofularia arguta

1973 ◽  
Vol 51 (2) ◽  
pp. 465-473 ◽  
Author(s):  
Emile Miginiac ◽  
Nicole Lacombe
Keyword(s):  
Growth Regulators ◽  
Acid Treatment ◽  
Culture Medium ◽  
Floral Development ◽  
Bud Development ◽  
Terminal Bud ◽  
Absorbing Roots

The influence of various correlations and a few growth regulators (auxin, abscissic acid, and kinetin) on vegetative or floral development of cotyledonary buds is studied on plants or plant fragments cultured in vitro. It is shown that the terminal bud inhibits growth and stimulates flowering, whereas "non-absorbing roots" inhibit floral development; if the terminal bud is present, the roots are inactive. This antagonism between organs can be simulated by replacing the terminal bud by an auxin or abscissic acid treatment and the roots by a kinetin treatment. The two groups of substances show antagonistic actions on the nature of cotyledonary bud development. Flowering or non-flowering, then, depends on the balance of concentration of these two groups of substances in the culture medium.

scholarly journals Handling culture medium composition for optimizing plant cell suspension culture in shake flasks

2017 ◽  
Vol 133 (1) ◽  
pp. 137-146 ◽  
Author(s):  
Tiago Fidemann ◽  
Gabriela Aparecida de Araujo Pereira ◽  
Tárik Reis Heluy ◽  
Rodrigo Boccoli Gallego ◽  
Mônica Rosa Bertão ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Plant Cell ◽  
Cell Suspension Culture ◽  
Culture Medium ◽  
Medium Composition ◽  
Plant Cell Suspension Culture ◽  
Plant Cell Suspension ◽  
Shake Flasks
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