scholarly journals Antibacterial Poly-Herbal Semisolid Formulations Containing Leaves Extracts of Tectona grandis, Mangifera indica and Anacardium occidentale

2016 ◽  
Vol 8 (05) ◽  
Author(s):  
K. Krishnananda Kamath ◽  
A. Shabaraya
Keyword(s):  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Tectona Grandis ◽  
Dosage Forms ◽  
Water Soluble ◽  
Diffusion Method ◽  
E Coli ◽  
Zones Of Inhibition ◽  
Herbal Formulations ◽  
Agar Well Diffusion Method

Herbal medicine have become an item of global importance both has medicinal and economical value. Plants used for medicinal purposes long before recorded history. Many plant species have been proved to have antibacterial activity. Thus, the main objective of the study was to formulate and evaluate a poly-herbal semisolid dosage forms using ethanolic extracts of frontal leaves of T. grandis, M. indica and A. occidentale. Formulations were evaluated for its physicochemical properties like color, consistency, pH, spreadability, extrudability and the results were found satisfactory. Antibacterial activity of formulations was studied against S. aureus, E. coli and P. aeruginosa by agar well diffusion method. (E. coli, P. aeruginosa) bacteria. The order of activity was as follows: Water Soluble bases > Gel Bases > Hydrocarbon Bases. The zones of inhibition of poly herbal formulations were in between 23-28 mm which can be comparable with standard formulation 24-29 mm. Activity of the formulations may be due to the presence of alkaloids, flavanoids, phenols and tannins in the extracts. The formulations were found to be very efficacious in all the parameters.

scholarly journals STUDY OF ANTIBACTERIAL ACTIVITY OF THE PREPARATIONS WITH DIOXIDINE AND LEVOFLOXACIN ON MAIN PURULENT-INFLAMMATORY PROCESSES PATHOGENS

2021 ◽  
Vol 74 (9) ◽  
pp. 2109-2111
Author(s):  
Evheniia A. Shtaniuk ◽  
Oleksandra O. Vovk ◽  
Larisa V. Krasnikova ◽  
Yuliia I. Polyvianna ◽  
Tetiana I. Kovalenko
Keyword(s):  
Antibacterial Activity ◽  
Water Soluble ◽  
Diffusion Method ◽  
E Coli ◽  
Mueller Hinton ◽  
Microbiological Methods ◽  
Pure Cultures ◽  
Inflammatory Processes ◽  
Definition Of

The aim: Study of antibacterial activity of the preparations, containing antiseptic dioxidine and antibiotic levofloxacin in vitro on standard strains of main optional-anaerobic pathogens of purulent-inflammatory processes of surgical wounds S. aureus, E. coli, P. aeruginosa and definition of more effective ones on them. Materials and methods: Solutions of dioxidine 1.2 %, dioxidine 1.2% with decamethaxin, Dioxisole, water soluble ointment with dioxidine 1.2% and levofloxacin 0.1% with decamethaxin were used in experiment. Antibacterial activity was studied on standard strains of S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Distinguishing and identification of pure cultures of bacteria was done according to generally accepted microbiological methods. Determination of purulent-inflammatory processes pathogens sensitivity was done by disco-diffuse method on Mueller-Hinton medium. Antibacterial activity of solutions and ointments was studied with the help of agar diffusion method (“well” method) according to methodic recommendations. Each investigation was repeated 6 times. Method of variation statistics was used for the research results analysis. Results: All antibacterial preparations under study are effective and highly effective on S. aureus АТСС 25923, E. coli АТСС 25922, P. aeruginosa АТСС 27853. Solution with 1.2 % dioxidine with decamethaxin and ointment with 0.1 % levofloxacin and decamethaxin have larger growth retardation zones towards S. aureus and P. aeruginosa. E. coli strains are more sensitive to the solution of Dioxisole and ointment with 1.2 % dioxidine. Conclusions: All strains are sensitive, most of them are highly sensitive, up to 5 antibacterial preparations under study in vitro.

scholarly journals ANTIBACTERIAL ACTIVITY OF SOAP MADE FROM ESSENTIAL OILS AND COMMERCIAL SOAP SOLD IN THE LEBANESE MARKET

2021 ◽  
pp. e304
Author(s):  
Candy GERGES ◽  
Mohamad HADLA ◽  
Michele ELIALI ◽  
Martine RICHA ◽  
Nour Mammari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Effective Measure ◽  
E Coli ◽  
Different Types ◽  
Commercial Soap ◽  
Zones Of Inhibition

Background: Thousands of people are dying as a result of infections caused by bacteria. Among the main routes of germ transmission are the hands, making hand hygiene very important in preventing the spread of pathogens and bacterial infection. Hand washing with soap and water is considered to be a simple and effective measure. Old soap manufacturers have long had traditional uses in the Lebanese community. Methods: The purpose of this study is to compare the antibacterial activity of oil-based soaps with commercial soap sold in the Lebanese market. Different types of herbal soap and antiseptics have been used in this study. Four bacterial strains were used: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter Spp. The antibacterial activities of these soaps were determined by the diffusion method of disks in agar medium. Results and Discussion: Statistical analysis of zones of inhibition showed that S. epidermidis, P. aeruginosa and Enterobacter Spp. were sensitive only to traditional oil-based soaps, which are Sage, Rose Mary and Cedar. In addition, S. aureus showed sensitivity to soaps comprised of essential oils as well as antibacterial synthetic soap, Dettol and Lifebuoy. On the other hand, E. coli showed resistance to all soaps. Soaps comprised of natural essential oils have shown antibacterial activity superior to so-called “Antibacterial” soaps. Conclusion: Based on this study, we can say that the use of soaps with essential oils might be the best option due to their organic origin as well as their antibacterial proved activities.

scholarly journals Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Luthfiah Luthfiah ◽  
Dwi Setyati ◽  
Sattya Arimurti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Well Diffusion Method

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.

scholarly journals ANTIBACTERIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF MANGIFERA INDICA ETHANOL AND AQUEOUS LEAVES EXTRACT AGAINST PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS

2021 ◽  
Vol 8 (12) ◽  
pp. 262-269
Author(s):  
M. A. Garga ◽  
U. M. Garasin ◽  
M. Abdullahi ◽  
B. A. Muhammed ◽  
A. Yakubu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Gram Staining ◽  
Significant Difference ◽  
Zones Of Inhibition ◽  
And Staphylococcus Aureus

The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.

scholarly journals COMPARISON OF ANTIBACTERIAL ACTIVITY OF LEAVES EXTRACTS OF TECTONA GRANDIS, MANGIFERA INDICA, AND ANACARDIUM OCCIDENTALE

2016 ◽  
Vol 9 (1) ◽  
pp. 36
Author(s):  
Krishnananda Kamath K. ◽  
A. Ramakrishna Shabaraya
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Mangifera Indica ◽  
Tectona Grandis ◽  
Anacardium Occidentale ◽  
World Health ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Morphological Studies

Objective: World Health Organization estimated that 80% of people worldwide rely upon herbal medicines for some aspect of their primary healthcare. For most of the herbs, the specific ingredient that causes therapeutic effect is not known. Bacterial infections are one of the prominent causes of health problems, physical disabilities and mortalities around the world. Plants have been used in medicine as antimicrobial agents since ancient times could provide a promising solution for drug-resistant species. The present study involves comparison of the antibacterial activity of ethanolic extract leaves of Tectona grandis (teak), Mangifera indica (mango), and, Anacardium Occidentale (cashew).Methods: Authentication, morphological studies and phytochemical screening studies on alcoholic extracts of leaves were carried out. Antibacterial activity was carried out by using different concentrations of extracts on bacterial strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa by using agar well diffusion method and results were compared.Results: Preliminary phytochemical screening of extracts revealed the presence of alkaloids, glycosides, saponins, resins, tannins and flavonoids. Antibacterial activity was observed in the concentration range of 25-100 mg/ml for all the extracts except T. grandis leaves. They are effective at 50-100 mg/ml concentration.Conclusion: Comparison of results reveals that leaves of T. Grandis have less antibacterial activity compared to M. Indica and A. Occidantale extracts.Keywords: Teak, Mango, Cashew Leaves Extracts, Phytochemical Screening, Antibacterial activity

scholarly journals PERBANDINGAN PENGUJIAN AKTIVITAS ANTIBAKTERI STARTER YOGURT DENGAN METODE DIFUSI SUMURAN DAN METODE DIFUSI CAKRAM

2020 ◽  
Vol 1 (2) ◽  
pp. 41
Author(s):  
Lilih Siti Nurhayati ◽  
Nadhira Yahdiyani ◽  
Akhmad Hidayatulloh
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Activity ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Testing Methods ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Agar Well Diffusion Method

Antibacterial activity testing can be done using the agar diffusion method, including agar well difussion and disk diffusion agar methods. This study aims to compare two antibacterial testing methods to analyze the anti-bacterial activity of a yogurt starter against the bacteria Eschericia coli and Staphilococcus aureus. The study was conducted experimentally with 5 concentrations of yogurt starter, namely 2%, 4%, 6%, 8%, and 10%. Testing antibacterial activity using two methods ,disk diffusion agar and well difussion agar methods. The research showed that agar well diffusion method obtained antibacterial activity greater than the disk diffusion method for E. coli and S. aureus.

Synthesis and Antibacterial Activities of Ag/ZnO Nanoparticles

2016 ◽  
Vol 697 ◽  
pp. 714-717
Author(s):  
Ya Wei Hu ◽  
Hui Rong He ◽  
Xia Kong ◽  
Yang Min Ma
Keyword(s):  
Antibacterial Activity ◽  
Zno Nanoparticles ◽  
Sol Gel ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Crystallographic Structure ◽  
Zno Nanoparticle ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Zones Of Inhibition

Ag/ZnO nanoparticles with different Ag concentrations were fabricated through sol-gel method under 500 °C for 60 min with a heating rate 5 °C·min-1. The phase, the crystallographic structure and the surface topography of the Ag/ZnO nanoparticles were charactered by XRD and SEM. The antibacterial activities of the Ag/ZnO nanoparticles were performed by disc diffusion method. The results indicate that the as-prepared Ag/ZnO nanoparticles display great antibacterial activity than ZnO nanoparticle without Ag doping. And Ag/ZnO nanoparticles with 5 mol% Ag concentration show more excellent antibacterial activity toward S.aureus, B. subtilis, E. coli and P. aeruginosa with diameters zones of inhibition 21.7, 18.5, 18.4 and 17.8 mm than other Ag/ZnO nanoparticles. And the mechanism of ZnO antibacterial activity is discussed.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Terpurifikasi Daun Mangga Arumanis (Mangifera indica L.) dan Identifikasi Flavonoid dengan KLT

2020 ◽  
Vol 6 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Dewi Andini Kunti Mulangsri ◽  
Elya Zulfa
Keyword(s):  
Antibacterial Activity ◽  
Mangifera Indica ◽  
Hot Water ◽  
Flavonoid Compound ◽  
Diffusion Method ◽  
Therapeutic Effects ◽  
E Coli ◽  
Flavonoid Compounds ◽  
Rf Values ◽  
Mango Leaves

Arumanis mango leaves (Mangifera indica L.) has antibacterial activity and antifungal, evidently. Escherichia coli and staphylococcus aureus are opprtunistic pathogen bacteria that can cause gastrointestinal and skin infection. Purification of extract was done to eliminate the presence of ballast substances that cannot produce therapeutic effects. This study aims to acknowledge the antibacterial activity of purified mango arumanis leaves extract (PMALE) against E. coli and S. aureus, flavonoid compound content by TLC. Arumanis Mango leaves powder was extracted with 96% ethanol solvent by maceration method, followed purification the extract was done with hot water-ethylacetate solvent by liquid-liquid extraction method. Antibacterial activity test was done by agar diffusion method against E. coli and S. aureus bacteria with the same serial concentration of 6.25; 12.5; 25; 50; 75 and 100% and Thin Layer Chromatography (TLC) tests for detection flavonoid compounds, the stationary phase used silica gel 60F 254 and the mobile phase used n-hexane-ethylacetate (1:3). The results of PMALE had antibacterial activity at lower concentrations 6,25% against both of E. coli and S. aureus. PMALE had flavonoid compounds with Rf values of 0.81.

scholarly journals Evaluation of the Antibacterial Activity of Gongronema latifolium and Costus afer Leaf Extracts on E. coli (ATCC 29455) and S. aureus (ATCC 25923)

2020 ◽  
pp. 11-16
Author(s):  
N. P. Akani ◽  
C. Nwachukwu ◽  
I. O. Hakam
Keyword(s):  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Diffusion Method ◽  
Sensitivity Testing ◽  
E Coli ◽  
Study Results ◽  
Ethanolic Extracts ◽  
Zones Of Inhibition ◽  
Gongronema Latifolium ◽  
Costus Afer

Man’s use of medicinal plants in treating illnesses is as old as human existence and many plants have been used for this purpose because of their phytochemical constituents that prove many times to be antimicrobial. The antibacterial activity of the leaf extract of Gongronema latifolium and Costus afer on Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25923) was investigated using standard microbiological procedures of sub-culturing, identity confirmation, water and ethanol extraction of leaves and sensitivity testing via agar well diffusion method. Results revealed that S. aureus and E. coli were both inhibited by the aqueous extract of C. afer with zone diameter of 16 mm and 15 mm respectively as well as the ethanolic extract of C. afer with diameter of 18mm and 15 mm respectively. However, aqueous and ethanolic extracts of G. latifolium proved ineffective against the strains of E. coli and S. aureus used in this study. Results of minimum inhibitory concentration revealed MIC of the aqueous extract of C. afer on E. coli and S. aureus to be 50 mgml-1 and 25 mgml-1 respectively while that of the ethanolic extracts of C. afer was 12.5 mgml-1 and 6.25 mgml-1 for E. coli and S. aureus respectively. Comparatively E. coli showed high sensitivity to Ciprofloxacin, Gentamycin and Septrin with zones of inhibition of 37, 32 and 24 respectively and resistant to Ampicillin, Erythromycin and Tetracycline with zones of inhibition of 6, 0 and 0 respectively. S. aureus on the other hand proved sensitive to Ciprofloxacin, Erythromycin, Gentamycin and Tetracycline with zones of inhibition of 35, 28, 29 and 34 respectively and resistant to Ampicillin and Septrin with zones of inhibition of o respectively. This study has revealed that some positive effect can be achieved against S. aureus and E. coli infections using C. afer at good concentrations. Better results could also be achieved using ethanol as extracting medium with instead of water as is common practice.

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

Sign in / Sign up

Export Citation Format

Share Document