Efficacy and safety of a modified-live cyprinid herpesvirus 3 vaccine in koi (Cyprinus carpio koi) for prevention of koi herpesvirus disease

2014 ◽  
Vol 75 (10) ◽  
pp. 899-904 ◽  
Author(s):  
E. P. Scott Weber ◽  
Kirsten V. Malm ◽  
Susan C. Yun ◽  
Lori A. Campbell ◽  
Philip H. Kass ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Efficacy And Safety ◽  
Koi Herpesvirus ◽  
Cyprinid Herpesvirus 3 ◽  
Cyprinus Carpio Koi

scholarly journals Kelangsungan Hidup Ikan Koi (Cyprinus carpio koi) Yang Terinfeksi KHV (Koi herpesvirus) [The Survival Of Koi Goldfish (Cyprinus carpio koi) Which Infected By KHV (Koi herpesvirus) ]

2019 ◽  
Vol 1 (2) ◽  
pp. 135
Author(s):  
Ninik Setyorini, Asmaul Khusnah, Lilia Widajatiningrum
Keyword(s):  
Cyprinus Carpio ◽  
Koi Herpesvirus ◽  
Cyprinus Carpio Koi ◽  
Pcr Test

AbstractThe research aim to know the capability of survival life of the infected KHV koi fish that proved with PCR test. The research held from 2003 until 2008, with the methode took gill sample from the same fish every 4 months. Result of PCR showed positive KHV in each analysis during 4.5 years. According to the PCR test for KHV that conducted for 4.5 years showed that the result always positive in every test. 

Characterization of a new cell line from caudal fin of koi, Cyprinus carpio koi, and first isolation of cyprinid herpesvirus 3 in China

2011 ◽  
Vol 161 (2) ◽  
pp. 140-149 ◽  
Author(s):  
Chuanfu Dong ◽  
Shaoping Weng ◽  
Wei Li ◽  
Xuezhu Li ◽  
Yang Yi ◽  
...  
Keyword(s):  
Cell Line ◽  
Cyprinus Carpio ◽  
Caudal Fin ◽  
New Cell Line ◽  
Cyprinid Herpesvirus 3 ◽  
Cyprinus Carpio Koi

Protective immunity of a modified-live cyprinid herpesvirus 3 vaccine in koi (Cyprinus carpio koi) 13 months after vaccination

2014 ◽  
Vol 75 (10) ◽  
pp. 905-911 ◽  
Author(s):  
Matthew R. O'Connor ◽  
Thomas B. Farver ◽  
Kirsten V. Malm ◽  
Susan C. Yun ◽  
Gary D. Marty ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Protective Immunity ◽  
Cyprinid Herpesvirus 3 ◽  
Cyprinus Carpio Koi

Non-lethal diagnostic methods for koi herpesvirus in koi Cyprinus carpio

2020 ◽  
Vol 138 ◽  
pp. 195-205 ◽  
Author(s):  
E Soto ◽  
E Tamez-Trevino ◽  
Z Yazdi ◽  
BN Stevens ◽  
S Yun ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Carrier State ◽  
Diagnostic Methods ◽  
Permissive Temperature ◽  
Characteristic Analysis ◽  
Viral Pathogen ◽  
Koi Herpesvirus ◽  
Cyprinid Herpesvirus 3 ◽  
Herpesvirus 1 ◽  
And Control

Cyprinid herpesvirus 3, also known as koi herpesvirus (KHV), is a viral pathogen responsible for mass mortalities of carp worldwide. In this study, we compared the sensitivity and specificity of ELISA and quantitative PCR (qPCR) methods for the diagnosis of KHV in experimentally infected koi Cyprinus carpio over an 11 mo period. Koi were exposed to KHV at 18 ± 1°C (permissive temperatures for KHV disease) in laboratory-controlled conditions. At 21 d post challenge, the temperature in the system was decreased to <15°C (non-permissive temperature for KHV disease), and fish were monitored for the following 11 mo. At different time points throughout the study, samples of blood and gills were collected from exposed and control koi and subjected to qPCR and ELISA. Survival proportions of 53.3 and 98.8% in exposed and control treatments, respectively, were recorded at the end of the challenge. Traditional receiver-operating characteristic analysis was used to compare the sensitivity of the ELISA and blood and gill qPCR during permissive and non-permissive temperatures. ELISA was superior to qPCR of gills and whole-blood samples in detecting previous exposure to KHV. Similar results were obtained in a second experiment exposing koi to KHV and inducing persistent infection at >30°C (non-permissive temperature for KHV disease). Finally, KHV ELISA specificity was confirmed using cyprinid herpesvirus 1-exposed koi through a period of 3 mo. This study demonstrates that the combination of ELISA and gill qPCR should be recommended in the diagnosis of KHV exposure of suspected carrier-state fish.

scholarly journals Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally-infected Cyprinus carpio koi as assessed by real-time TaqMan PCR

2004 ◽  
Vol 60 ◽  
pp. 179-187 ◽  
Author(s):  
O Gilad ◽  
S Yun ◽  
FJ Zagmutt-Vergara ◽  
CM Leutenegger ◽  
H Bercovier ◽  
...  
Keyword(s):  
Real Time ◽  
Cyprinus Carpio ◽  
Koi Herpesvirus ◽  
Taqman Pcr ◽  
Cyprinus Carpio Koi

Detection of koi herpesvirus (KHV) from koi (Cyprinus carpio koi) broodstock in South Korea

Aquaculture ◽  
2011 ◽  
Vol 311 (1-4) ◽  
pp. 42-47 ◽  
Author(s):  
Dennis K. Gomez ◽  
Seong Joon Joh ◽  
Hwan Jang ◽  
Sang Phil Shin ◽  
Casiano H. Choresca ◽  
...  
Keyword(s):  
South Korea ◽  
Cyprinus Carpio ◽  
Koi Herpesvirus ◽  
Cyprinus Carpio Koi

scholarly journals Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi

2003 ◽  
Vol 84 (10) ◽  
pp. 2661-2667 ◽  
Author(s):  
Oren Gilad ◽  
Susan Yun ◽  
Mark A. Adkison ◽  
Keith Way ◽  
Neil H. Willits ◽  
...  
Keyword(s):  
Water Temperature ◽  
Cyprinus Carpio ◽  
Optimal Growth ◽  
Rapid Onset ◽  
Minimal Growth ◽  
Koi Herpesvirus ◽  
Cyprinus Carpio Carpio ◽  
Molecular Comparison ◽  
Group A ◽  
Cyprinus Carpio Koi

Koi herpesvirus (KHV) has been associated with devastating losses of common carp (Cyprinus carpio carpio) and koi (Cyprinus carpio koi) in North America, Europe, Israel and Asia. A comparison of virion polypeptides and genomic restriction fragments of seven geographically diverse isolates of KHV indicated that with one exception they represented a homogeneous group. A principal environmental factor influencing the onset and severity of disease is water temperature. Optimal growth of KHV in a koi fin cell line occurred at temperatures from 15–25 °C. There was no growth or minimal growth at 4, 10, 30 or 37 °C. Experimental infections of koi with KHV at a water temperature of 23 °C resulted in a cumulative mortality of 95·2 %. Disease progressed rapidly but with lower mortality (89·4–95·2 %) at 28 °C. Mortality (85·0 %) also occurred at 18 °C but not at 13 °C. Shifting virus-exposed fish from 13–23 °C resulted in the rapid onset of mortality.

Immersion immunization of koi (Cyprinus carpio) against cyprinid herpesvirus 3 (CyHV-3) with carbon nanotube-loaded DNA vaccine

Aquaculture ◽  
2021 ◽  
Vol 539 ◽  
pp. 736644
Author(s):  
Feng Hu ◽  
Yingying Li ◽  
Qing Wang ◽  
Bin Zhu ◽  
Siyu Wu ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Dna Vaccine ◽  
Cyprinus Carpio ◽  
Cyprinid Herpesvirus 3
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