scholarly journals Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally-infected Cyprinus carpio koi as assessed by real-time TaqMan PCR

2004 ◽  
Vol 60 ◽  
pp. 179-187 ◽  
Author(s):  
O Gilad ◽  
S Yun ◽  
FJ Zagmutt-Vergara ◽  
CM Leutenegger ◽  
H Bercovier ◽  
...  
Keyword(s):  
Real Time ◽  
Cyprinus Carpio ◽  
Koi Herpesvirus ◽  
Taqman Pcr ◽  
Cyprinus Carpio Koi

scholarly journals Kelangsungan Hidup Ikan Koi (Cyprinus carpio koi) Yang Terinfeksi KHV (Koi herpesvirus) [The Survival Of Koi Goldfish (Cyprinus carpio koi) Which Infected By KHV (Koi herpesvirus) ]

2019 ◽  
Vol 1 (2) ◽  
pp. 135
Author(s):  
Ninik Setyorini, Asmaul Khusnah, Lilia Widajatiningrum
Keyword(s):  
Cyprinus Carpio ◽  
Koi Herpesvirus ◽  
Cyprinus Carpio Koi ◽  
Pcr Test

AbstractThe research aim to know the capability of survival life of the infected KHV koi fish that proved with PCR test. The research held from 2003 until 2008, with the methode took gill sample from the same fish every 4 months. Result of PCR showed positive KHV in each analysis during 4.5 years. According to the PCR test for KHV that conducted for 4.5 years showed that the result always positive in every test. 

Efficacy and safety of a modified-live cyprinid herpesvirus 3 vaccine in koi (Cyprinus carpio koi) for prevention of koi herpesvirus disease

2014 ◽  
Vol 75 (10) ◽  
pp. 899-904 ◽  
Author(s):  
E. P. Scott Weber ◽  
Kirsten V. Malm ◽  
Susan C. Yun ◽  
Lori A. Campbell ◽  
Philip H. Kass ◽  
...  
Keyword(s):  
Cyprinus Carpio ◽  
Efficacy And Safety ◽  
Koi Herpesvirus ◽  
Cyprinid Herpesvirus 3 ◽  
Cyprinus Carpio Koi

scholarly journals APLIKASI KUANTIFIKASI KOI HERPESVIRUS : REAL TIME – QUANTITATIVE POLYMERASE CHAIN REACTION (RT-Q PCR) MENGGUNAKAN SYBR GREEN PADA IKAN MAS (Cyprinus carpio)

2017 ◽  
Vol 12 (1) ◽  
pp. 45
Author(s):  
Isti Koesharyani ◽  
Lila Gardenia ◽  
Tatik Mufidah ◽  
Ayi Santka
Keyword(s):  
Polymerase Chain Reaction ◽  
Viral Load ◽  
Real Time ◽  
Cyprinus Carpio ◽  
Quantitative Polymerase Chain Reaction ◽  
Sybr Green ◽  
Chain Reaction ◽  
Koi Herpesvirus ◽  
Ct Value ◽  
Polymerase Chain

Koi Herpes Virus (KHV) di Indonesia sejak tahun 2002 merupakan penyakit mematikan yang menyerang ikan koi Cyprinus carpio koi dan ikan mas Cyprinus carpio carpio, dan sampai saat ini, infeksi KHV dilaporkan sudah menyebar hampir di seluruh dunia. Untuk mengetahui adanya infeksi KHV perlu cara diagnosa yang sangat akurat/sensitif, sehingga keberadaan KHV dapat diketahui secara pasti dengan tingkat sensitivitas yang lebih baik pada ikan budidaya. Tujuan dari penelitian ini adalah untuk mengaplikasikan teknik deteksi dengan real time quantitative polymerase chain reaction (RT- qPCR/qPCR) guna mengetahui adanya infeksi KHV secara kuantitatif pada ikan mas dengan mengetahui kandungan virus (viral load). Sebanyak masing-masing 3 ekor sampel diperoleh dari sentra budidaya ikan mas di Cirata-Jawa Barat, Maninjau-Sumatera Barat, dan Banjarmasin-Kalimantan Selatan. Sampel-sampel tersebut selanjutnya dianalisa keberadaan KHV-nya dengan RT-qPCR menggunakan SYBR Green. Hasil pengujian menunjukkan bahwa jumlah tertinggi (viral load) diperoleh dari ikan mas asal Cirata-3 dengan nilai Threshold Cycle (Ct.) 18,24 atau setara dengan 3,4 x 107 kopi, dan terendah dari ikan mas asal Banjarmasin-3 dengan nilai Ct. 33,39 atau 1,8 x 102 kopi. Dua standar yang digunakan dalam pengujian ini berupa plasmid dengan jumlah kopi 2 x 104 (Ct 27,24) dan 2 x 103 (Ct 30,24) dan kontrol atau Non Template Control (NTC) adalah 3,1 x 10 atau dengan nilai Ct 35,65. Uji aplikasi deteksi KHV dengan metode RT-qPCR ini memberikan hasil yang lebih sensitif, di mana sampel yang tidak terdeteksi dengan metode PCR konvensional dapat dideteksi dan dihitung jumlah kopi DNA (DNA copy). Since 2002, Koi herpesvirus (KHV) in Indonesian has been a malignant diseases, now recognized as a worldwide cause of mortality among populations of koi Cyprinus carpio koi and common carp Cyprinus carpio carpio. To determine the presence of infection is required the KHV diagnosis method with highly accurate and sensitive, so that the existence KHV can be known exactly with high sensitivity level in fish farming.The objective of this study was to develop and evaluate the infection by Real Time Quantitative Polymerase Chain Reaction (RT- qPCR/qPCR). Sample were taken from Carp culture in West Java, West Sumatra, and South Kalimantan. The assay was done by SYBR Green RT-qPCR. The analysis result of KHV in carp revealed that the carp from Cirata-3 had the highest viral load with Ct. value 18.24 equal with 3.4 x 107 copies, and the lowest one was the carp from Banjarmasin-3 at Ct. value 33.39 (1.8 x 102 copies), while two standards plasmid and Non Template Control (NTC) had Ct value of 27.24 (2 x 104copies),30.24 (2 x 103copies), and 35.65 (3.1 x 10 copies), respectively. Application KHV test by q-PCR has more advantages and sensitive than that of conventional PCR, and it can be used to detect and calculate the copy number of DNA.

Detection of koi herpesvirus (KHV) from koi (Cyprinus carpio koi) broodstock in South Korea

Aquaculture ◽  
2011 ◽  
Vol 311 (1-4) ◽  
pp. 42-47 ◽  
Author(s):  
Dennis K. Gomez ◽  
Seong Joon Joh ◽  
Hwan Jang ◽  
Sang Phil Shin ◽  
Casiano H. Choresca ◽  
...  
Keyword(s):  
South Korea ◽  
Cyprinus Carpio ◽  
Koi Herpesvirus ◽  
Cyprinus Carpio Koi

scholarly journals Molecular comparison of isolates of an emerging fish pathogen, koi herpesvirus, and the effect of water temperature on mortality of experimentally infected koi

2003 ◽  
Vol 84 (10) ◽  
pp. 2661-2667 ◽  
Author(s):  
Oren Gilad ◽  
Susan Yun ◽  
Mark A. Adkison ◽  
Keith Way ◽  
Neil H. Willits ◽  
...  
Keyword(s):  
Water Temperature ◽  
Cyprinus Carpio ◽  
Optimal Growth ◽  
Rapid Onset ◽  
Minimal Growth ◽  
Koi Herpesvirus ◽  
Cyprinus Carpio Carpio ◽  
Molecular Comparison ◽  
Group A ◽  
Cyprinus Carpio Koi

Koi herpesvirus (KHV) has been associated with devastating losses of common carp (Cyprinus carpio carpio) and koi (Cyprinus carpio koi) in North America, Europe, Israel and Asia. A comparison of virion polypeptides and genomic restriction fragments of seven geographically diverse isolates of KHV indicated that with one exception they represented a homogeneous group. A principal environmental factor influencing the onset and severity of disease is water temperature. Optimal growth of KHV in a koi fin cell line occurred at temperatures from 15–25 °C. There was no growth or minimal growth at 4, 10, 30 or 37 °C. Experimental infections of koi with KHV at a water temperature of 23 °C resulted in a cumulative mortality of 95·2 %. Disease progressed rapidly but with lower mortality (89·4–95·2 %) at 28 °C. Mortality (85·0 %) also occurred at 18 °C but not at 13 °C. Shifting virus-exposed fish from 13–23 °C resulted in the rapid onset of mortality.

Quantification of the biocontrol agent Trichoderma harzianum with real-time TaqMan PCR and its potential extrapolation to the hyphal biomass

2010 ◽  
Vol 101 (8) ◽  
pp. 2888-2891 ◽  
Author(s):  
Rubén López-Mondéjar ◽  
Anabel Antón ◽  
Stefan Raidl ◽  
Margarita Ros ◽  
José Antonio Pascual
Keyword(s):  
Real Time ◽  
Trichoderma Harzianum ◽  
Biocontrol Agent ◽  
Taqman Pcr

Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples

2017 ◽  
Vol 34 ◽  
pp. 53-55 ◽  
Author(s):  
Marco Ligozzi ◽  
Erica Diani ◽  
Federica Lissandrini ◽  
Rosanna Mainardi ◽  
Davide Gibellini
Keyword(s):  
Real Time ◽  
Human Bocavirus ◽  
Taqman Pcr ◽  
Ns1 Gene
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