scholarly journals GC-MS Analysis of Phytochemical Constituents in Vitis Vinifera and Hylocereus Undatus

2017 ◽  
Vol 7 (5) ◽  
pp. 7-12
Author(s):  
K. Sudha et al., K. Sudha et al., ◽  
Keyword(s):  
Vitis Vinifera ◽  
Ms Analysis ◽  
Phytochemical Constituents ◽  
Hylocereus Undatus

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY AND GC-MS ANALYSIS OF THE ETHANOLIC EXTRACTS OF TERMINALIA BELLERICA ROXB (BAHEDA)

2016 ◽  
Vol 8 (11) ◽  
pp. 275
Author(s):  
Rasna Gupta ◽  
Ram Lakhan Singh ◽  
Neeraja Dwivedi
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Terminalia Bellerica ◽  
Ms Analysis ◽  
Phytochemical Constituents

Objective: To investigate the antioxidant activity of ethanolic extract of Terminalia bellerica fruit pulp (TBFP), seed (TBS) and bark (TBB), and identification of phytochemical constituents of said extracts.Methods: The antioxidant potential of T. bellerica (TB) parts was evaluated by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), hydroxyl radical scavenging activity (HRSA) and compared with reference standard quercetin. Lipid peroxidation (LPO), ferric thiocyanate activity (FTC) and reducing power (RP) of the plant extracts were also examined. The phytochemical constituents of said extracts have been quantified by gas chromatography-mass spectrometer (GC-MS) method.Results: Ethanolic extract of TBFP exhibited high phenolic content (254.33) followed by TBS (227.33) and TBB (185) mg/g of GAE. TBFP showed lowest IC50 for FRSA (62 µg/ml), SARSA (39 µg/ml) and HRSA (27 µg/ml) and highest RP (3.39 ascorbic acid equivalents/ml). TBB showed lowest IC50 for LPO (48 µg/ml) whereas TBS showed lowest IC50 for FTC (109 µg/ml). Predominant phyto-compounds present in TB extracts were quinic acid, gallic acid, ethyl galate, 9, 12 octadecadienoic acid and glucopyranose in varying concentrations as analyzed by GC-MS.Conclusion: On the basis of correlation of antioxidant studies with total phenolic content (TPC) and GC-MS analysis of different part of TB, it may be concluded that high concentration of total phenolics and other bioactive phytochemicals of TB extracts make it a potential source of nutraceutical antioxidants.

scholarly journals PHYTOCHEMICAL STUDIES AND GC-MS ANALYSIS OF SPERMADICTYON SUAVEOLENS ROXB

2017 ◽  
Vol 9 (3) ◽  
pp. 143 ◽  
Author(s):  
Ruthiran Papitha ◽  
Lokesh Ravi ◽  
Chinnadurai Immanuel Selvaraj
Keyword(s):  
Functional Groups ◽  
Mass Spectra ◽  
Ultra Violet ◽  
Chloroform Extract ◽  
Leaf Extracts ◽  
Ir Spectrometry ◽  
Ms Analysis ◽  
Ft Ir ◽  
Phytochemical Constituents ◽  
Phytochemical Studies

Objective: The present study was performed to identify the phytochemical constituents of leaves and flowers of a plant Spermadictyon suaveolens extracted with four different solvents.Methods: Dried and powdered samples were subjected to soxhlation based on the polarity of the solvents. The extracts were scanned using Ultra Violet-visible (UV-Vis) spectrophotometry with the wavelength ranging from 200–800 nm by comparing the absorption spectrum with the spectra of known compounds, Fourier Transform Infrared (FT-IR) spectrometry was used to find out the functional groups of the compounds and GC-MS system consisting of a Perkin Elmer Technologies Model Clarus 680 GC equipped with Clarus 600 (EI) was used to identify the metabolites by matching their recorded mass spectra with the standard mass spectra from National Institute of Standards and Technology (NIST05. LIB) libraries provided by the software of the GCMS system (TurboMass version 5.4.2).Results: The phytochemical tests indicated the presence of carbohydrates, alkaloids, flavonoids, phenols, tannins, saponins and terpenoids from the chloroform extract of leaves and flowers. UV-visible spectrophotometer results indicated a wavelength range between 230–660 nm for the flower and leaf extracts for major peaks. FT-IR analysis indicated major functional groups such as aromatic, primary, secondary and aliphatic amines, alkanes, carboxylic acids and amides. GC-MS analysis results revealed major bioactive compounds in the crude extracts.Conclusion: Presence of secondary metabolites has been identified from the phytochemical studies. Many phyto-compounds have been identified from the leaves and flowers of using GC-MS analysis. Hence, this medicinal plant may be used as a source for treating many diseases.

scholarly journals Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 90-94
Author(s):  
P Sasikala ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
M Padma ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Fine Powder ◽  
Ethanol Extract ◽  
Taraxacum Officinale ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Biological Compounds ◽  
Phytochemical Compounds ◽  
Medicinal Properties ◽  
Phytochemical Constituents

Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds. Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis. Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial  and antidiabetic compounds. Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various  medicinal  properties to cure dangerous diseases and disorders. Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds.

GC-MS ANALYSIS OF HEXANE: CHLOROFORM FRACTION OF INDIGOFERA TRITA L. F.

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (02) ◽  
pp. 15-19
Author(s):  
J. Aranganayaki ◽  
◽  
G. Jayanthi
Keyword(s):  
Gas Chromatography ◽  
Mass Spectroscopy ◽  
Phthalic Acid ◽  
Chemical Constituents ◽  
Chloroform Fraction ◽  
Ms Analysis ◽  
Phytochemical Constituents ◽  
Major Chemical ◽  
Gas Chromatography Mass Spectroscopy

In the present study, the hexane: chloroform fraction of Indigofera trita has been subjected to gas chromatography mass spectroscopy (GC-MS) analysis. Thirty phytochemical constituents have been identified. The major chemical constituents are phthalic acid, di(2-propylpentyl) ester, hexadecane, 1-[trans-4-(1-fluoro-2-iodoethyl)cyclohexyl]-trans-4-(3,4-difluorophenyl) cyclohexane, eicosane and 1- methyl-1-(allyloxy)-1-silacopent-3-ene.

GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) ANALYSIS OF PHYTOCHEMICALS OF FIRST GENERATION GAMMA-IRRADIATED TYPHONIUM FLAGELLIFORME LODD. MUTANTS

2016 ◽  
Vol 78 (10-4) ◽  
Author(s):  
Nesti Fronika Sianipar ◽  
Ragapadmi Purnamaningsih ◽  
Ireng Darwati ◽  
Danny Laurent
Keyword(s):  
Mass Spectrometry ◽  
Genetic Diversity ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Hexadecanoic Acid ◽  
Anticancer Compounds ◽  
Ethyl Palmitate ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Phytochemical Constituents

Rodent tuber (Typhonium flagelliforme Lodd.) is an Indonesian herbal plant from Araceae family which is highly potential against several types of cancer. However, anticancer compound of rodent tuber is currently unknown. Rodent tuber has a low genetic diversity due to conventional vegetative propagation. In vitro propagation combined with gamma-irradiation of rodent tuber’s calli had been performed to increase the genetic diversity of rodent tuber. The mutant plants had been acclimatised and analysed with RAPD molecular markers, but the phytochemical constituents of these mutants have never been investigated. This research utilised gas chromatography-mass spectrometry (GC-MS) analytical method to identify and measure the relative abundances of major phytochemical constituents of rodent tuber control and mutant plants. GC-MS analysis successfully showed phytochemical constituents of the ethanol extract of rodent tuber plants. Shoots and tubers of mutant clones had at least 8 anticancer compounds whose quantities were higher than control plants. Shoots and tubers of mutant clones also contained new anticancer compounds which were not found in control plants. Shoots of mutant clones contained new anticancer compounds such as 7-pentadecyne, β-sitosterol, hexadecanoic acid methyl ester, cis-vaccenic acid, ergost-5-en-3-ol (campesterol). Tubers of mutant clones contained new anticancer compounds such as β-sitosterol, ethyl palmitate, hexadecanoic acid ethyl ester, vitamin E (alpha tocopherol), ergost-5-en-3-ol (campesterol). Rodent tuber mutant clones are therefore very potential to be developed as anticancer drugs.

scholarly journals Identification of Chemical Compounds from the Ethanolic Extract of the Bark of Bauhinia tomentosa L. By GC-MS Analysis

2016 ◽  
Vol 8 (03) ◽  
Author(s):  
Gopalakrishnan S. ◽  
Vadivel E.
Keyword(s):  
Mass Spectra ◽  
Skin Diseases ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Microbial Infections ◽  
Phytochemical Constituents ◽  
Major Chemical

The bark of Bauhinia tomentosa L. is used wildly used for the treatment of varies ailments such as inflammation, wound, dysentery, skin diseases and for microbial infections. In the present study the ethanolic extract of the bark of Bauhinia tomentosa L. has been subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST) library. GC-MS analysis revealed the presence of fourteen phytochemical constituents, These compounds were identified by comparing their retention time and peak area with that of literature and by interpretation of mass spectra. The major chemical constituents are 4-(Benzyloxy)-1-methoxy-2(3,7-dimethylocta-2,6-dienyl)-benzene (10.88%), Levodopa (6.39%) and (9E, 12E) - Methyl octadeca-9,12-dienoate (5.68%) which possess many biological activities. Hence these studies pave a platform to screen many bioactive components for wound healing.

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