scholarly journals Chemical Analysis and the Study of Quality Indicators of the Immunobiological Drug for Preventing and Treating Candidal Infections

2020 ◽  
Vol 14 (4) ◽  
pp. 455-462
Author(s):  
Mykola Rybalkin ◽  
◽  
Leonid Strelnikov ◽  
Oksana Strilets ◽  
Olha Kaliuzhnaia ◽  
...  

Quality indicators such as protein content, polysaccharide content, phenol content, and pH of the developed vaccine solution for injection (VSI) “Candidocyde” based on cells of C. albicans and C. tropicalis fungi have been found to meet the standard requirements. It was determined that by the indicator of transparency and degree of turbidity the test solution is transparent, by the coloration degree it is colorless, by the sterility indicator it is sterile. The sterility period, determined after the first opening, is 8 hours. It was found that no solid particles were present in the solution; the container with the solution is hermetic; the volume of the container with a solution is not less than 5.0 ml. The test solution is stable for 2 years of storage at 275-265 K.

Author(s):  
Florin IMBREA ◽  
Branko MARINCOVIC ◽  
Valeriu TABĂRĂ ◽  
PAUL PÎRŞAN ◽  
Gheorghe DAVID ◽  
...  

Experimenting new technology of cultivating maize is an important step forward in order to optimise the yielding capacity if a crop that ranks second among crops cultivated worldwide and first among crops cultivated in Romania. Using low frequency radiations to stimulate yield and quality in maize allows increases in yield between 10 and 15% compared to the classical cultivation method and an improvement of the quality indicators (protein content increased with 6-11% determining an increase of the protein yield per ha; starch content increased with 7-14%, which also determined an increase of the starch yield per ha; while fat content, another indicator we monitored, increased with 2-6%).


2021 ◽  
Vol 13 (31) ◽  
pp. 17-22
Author(s):  
Rumen Bazitov ◽  
◽  
Milena Mihaylova ◽  

An experiment was conducted with maize for silage as a main irrigation culture in the experimental field of the Agricultural Institute - Stara Zagora. The following variations have been tested: variation 1 - no irrigation (sentinel); variation 2 - optimal irrigation, 80%-85% of FC (100% irrigation); variation 3 - Irrigation as variation 2 but with first irrigation cancelled; variation 4 - irrigation as variation 2 but with second irrigation cancelled; variation 5 - irrigation as variation 2, but with third irrigation cancelled. On the basis of a chemical analysis of the sudangrass forage for the raw protein content, FUM, FUG and PDI were defined. It was found that the highest yield of raw maize protein grown as the main crop was obtained from the optimal irrigation variation, both in the non-fertilized and the fertilized variation, respectively with 1023.5 kg / ha and 1303.5 kg / ha. The optimal water supply of maize provides the highest energy efficiency of the forage expressed in FUM -15022.8 kg / ha, FUG -15584.4 kg / da, PDI - 1060 kg / ha for non fertilized variations and FUM -16873.5 kg / ha, FUG -17516.3 kg / ha, PDI-1219 kg / ha with fertilizer applied.


2020 ◽  
pp. 120-129
Author(s):  
Vitali Zhemoida ◽  
Oleksandr Makarchuk ◽  
Roman Spriazhka

The basis of a complex and long process of creating new maize hybrids is the selection of parent components that can serve as sources of improved biochemical and economically valuable traits. Thus, depending on the peculiarities of the directions of maize grain processing, the production requires hybrids with a high starch content, and in the starch itself – amylose or amylopectin. When used for fodder purposes, high protein content is a must. This distribution of hybrids by areas of use will increase the profitability of production and reduce the cost of final products. The article presents the evaluation results of self-pollinating maize lines collection, which has 38 samples. The collection includes selection samples of the National University of Life and Environmental Sciences of Ukraine, Institute of Plant Breeding. V.Ya. Yuriev NAAS and lines of Canadian and Russian origin which are obtained from the National Center for Plant Genetic Resources of Ukraine. Field research was conducted according to generally accepted methods in the research fields of the Department of genetics, breeding and seed production. prof. M.O. Zelensky National University of Life and Environmental Sciences of Ukraine of the production unit "Agronomic Research Station", which is located in Vasylkiv district of Kyiv region laboratory. The soil of the experimental area is typical, low-humus, coarse-grained-medium-loam chernozem in terms of granulometric composition. Weather conditions were favorable for maize growing. Determination of quality indicators of maize grain was carried out on the «Infratec 1241 Grain Analyzer». The samples were divided into groups according to the content of quality indicators in the grain. In terms of protein content, two groups with high and medium content were formed, and the total variation of the indicator ranged from 10.0 to 13.8%. According to the starch content, the samples were also divided into two groups with very high and high content, and the percentage varied in the range of 66.5-71.8%. The range of oil content in the grain was in the range of 3.0-5.9% and, accordingly, three groups with high, medium and low content were formed. Selected inbred lines according to a set of indicators that serve as analyzers in the scheme of tester crosses to determine the degree of inheritance of specific biochemical traits. Key words: corn, inbred line, hybrid, biochemical parameters, amylose, amylopectin, tester, combination ability.


2021 ◽  
Vol 20 (4) ◽  
pp. 4-10
Author(s):  
A. Penaki ◽  
A. Borta

The results of studies of the quality of food and non-food grain of wheat from the southern regions of Ukraine in 2016 harvest are presented. The indicators of the quality of wheat grain were determined, the uniformity of their distribution in different classes of wheat was assessed, the content of protein fractions of the gluten complex and the molecular weights of individual protein structures were determined. It was found that the studied 13 samples of wheat grain had the following quality indicators: moisture content 9.4 ... 13.5%, bulk density 731 ... 814 g/l, protein content 10.1 ... 13.2%, gluten content 16.4 ... 25.6%, gluten quality 50 ... 110 units of the IDK device, falling number 311 ... 493 s, grain admixture 1.2 ... 10.5%, grains damaged by a wheat bug, 0.1 ... 10.1%. It was noted that the bulk density of all samples of wheat of 2 ... 6 classes exceeded the standard values in each class. In terms of protein content, 2 from 3 samples of class 5 wheat and 1 from 2 samples of 6 class exceeded the standard value of class 3 wheat (≥11.0%). The same was observed for these samples and for the gluten content (≥18%). Almost all grain samples, except for 2 samples of the 5th class and 2 samples of the 6th class, met the requirements of wheat of the 2nd class (45 ... 100 units device VDK). The falling number of all samples was high and significantly exceeded the normalized value (180 s), even for class 2 wheat. It was found that the uniformity of the distribution of individual quality indicators in different classes of wheat grain by the coefficient of variation is not the same. Such indicators of grain quality as moisture content, bulk density and protein content in different samples of the south of Ukraine in 2016 harvest fluctuated weakly (V≤10%), in terms of the amount of gluten and the falling number fluctuations were average (10% ≤V≤20%). Fluctuations in the content of grain impurities and grains damaged by the wheat bug are big (V≥20%). For the last indicator, fluctuations relative to the average are very large and exceed 120 %. This indicates that in different areas where wheat was grown, the infestation by the wheat bug was very different. It was shown that regardless of the total amount of protein determined by the Infratec FOSS express analyzer, four protein fractions of the gluten complex (albumins, globulins, gliadins and glutenins) are present in all grain classes, and the molecular weights of the protein structures of the main fractions are within small limits, which are in kDa - for albumins and globulins 20...30, gliadins 27.. 100 and glutenins 30...35. It was noted that in the varietal wheat "Chernobrova" albumins and globulins have a slightly higher molecular weight (30...40 kDa) than in non-varietal wheat.


Author(s):  
V. Divyabharathi ◽  
V. Swaminathan ◽  
P. Paramaguru ◽  
K. Venkatesan ◽  
T. Anitha ◽  
...  

A trial was conducted to assess the physiological and biochemical changes in M. oleifera (L.) Millsp.cv. PKM 1 induced by heading back and pinching treatments. Chlorophyll content, nitrate reductase content, soluble protein content and total phenol content were assessed after the new shoot initiated from heading back and pruning treatments before the flower initiation. Height and stage at which the apical growth was arrested significantly influenced chlorophyll, nitrate reductase, protein and phenol content in leaves. Heading back at 70 cm combined with pinching 100 days after heading back greatly influenced the physiological and biochemical factors except for soluble protein as it was increased by heading back at 30 cm.


2020 ◽  
pp. 108-118
Author(s):  
Olha Matsera

It is made the comparison estimation of winter rapeseed quality properties depending on the fertilization level and sowing date in this article. The literature on the research problem is elaborated; there are presented the study importance and relevance of growing technology elements influence on the quality properties of winter rapeseed seeds. It is analyzed the results of sowing periods and different rates of mineral fertilizers influence on the quality indicators of winter rapeseed seeds under the conditions of Right-Bank Forest-Steppe in Ukraine. Significant influence of the studied elements of technology on the main indicators of seed quality was noted; it was found that the change in the yield level obtained by the hybrids led to a change in the oil quality indices. Four the most important oil quality indicators were analyzed in this article. They are: acid value, glucosinolates amount, oil and protein contents. They were affected by different fertilizer rates and sowing dates, which were the experiment conditions. So, the lowest acid value – 1.08 provided hybrid Excel, when fertilizer rate was N240P120K24; when sowing dates were analyzed, the lowest indicator – 1.24 was obtained by Exagon hybrid in the first sowing date – 10th of Aug. the correlation between acid value and rapeseed yield was negative and oscillated from r = - 0.9327 to r = - 0.9843; the magnitude of the approximation reliability oscillated in the range of R2 = 0.87 – 0.97, which evidence the strong dependence between indicators. The lowest content of glucosinolates – 12.51 μmol / g had Excel hybrid under the conditions without fertilizers; when sowing dates were analyzed, the lowest indicator 15.06 μmol / g was obtained by Exagon hybrid in the first sowing date – 10th of Aug. The highest oil content had Excel hybrid – 46.27%, it was obtained in variant of N240P120K240. The protein content of each hybrid was increasing with fertilizer rates expansion. So, the highest indicator of it was obtained in the variant where N240P120K240 was used and it was Exagon – 22.10%. The highest protein content – 21.13% was obtained by Excel hybrid in the second sowing date – the 21st of Aug. when sowing dates were analyzed, and the highest oil content – 46.75% were obtained by Excel hybrid in the third sowing date – 5th of Sept. Key words: winter rapeseed, sowing dates, fertilizers, acid value, glucosinolates amount, oil and protein contents.


2021 ◽  
pp. 231-242
Author(s):  
Kavitha G ◽  
Mahalakshmi MS ◽  
K. Bayyapu Reddy ◽  
Y. Pushpa Reni ◽  
K. Radhika

A study was conducted to know the influence of physical and biochemical parameters on development of the pulse bruchid in green gram at RARS, Lam during 2017-18. The physical parameters of seed viz., colour, shape, surface texture, seed length and width, seed coat hardness and 100 seed weight and biochemical parameters such as protein content, phenol content and total sugars were evaluated for twelve genotypes of greengram. The greengram genotypes with smooth texture, oblong or globose shape and light coloured seed might be less preferred by the pulse bruchid for egg laying. The data showed that genotypes having low sugar and protein contents and high phenol content were resistant to pulse bruchid. The correlation studies showed that biological parameters i.e., number of eggs, adult emergence and growth index had significant positive association with protein content, sugar content, moisture content and electrical conductivity and negative correlation phenol content, 100 seed weight and seed coat hardness. In contrast, mean development period had negative association with protein content, sugar content, electrical conductivity and moisture content and positive correlation with phenol content, 100 seed weight and seed coat hardness. Multiple linear regression studies revealed that all the physical and biochemical properties of seed together were contributing to a large and significant variation (65 to 87 %) in growth parameters of pulse bruchid.


Author(s):  
I.N. Voronchikhina ◽  
◽  
V. S. Rubets ◽  
Y.N. Kotenko ◽  
V. V. Pylnev

The article presents the results of a study of 14 samples of spring soft wheat from the CIMMYT collection (Mexico) in the conditions of the Moscow region in 2020. It was found that all studied varieties are not suitable for breeding for yield. Samples numbered 1, 24, 41, 107, 178 and 214 have complex resistance to lodging and major fungal diseases. In terms of protein content, sample No. 87 turned out to be the most valuable in terms of quality. We recommend including the above varieties in breeding programs, since they have good yields along with high quality indicators.


2019 ◽  
Vol 9 (3) ◽  
pp. 104-113
Author(s):  
Cherifi Fadila ◽  
Kaid Harche Meriem

Argania spinosa (L.) Skeels. (Argan) is tropical species, this plant is known bythese therapeutic and medecinal uses. For this purpose, we test antibacterial potential of leaf extracts of three Algerian population of Argan trees andwe evaluate the geographical distribution and solvent extraction effect onthe antibacterial activities. In first, Levels of total phenol content, total protein content of leaf extracts were determined by UV-spectrophotometer,secondary, The agar well diffusion method were used to determine enzymeactivities of proteins, antibacterial effect and the minimal inhibitory concentration (MIC) of extracts. The amounts of the phenol and protein concentrations in tested extracts were in ranged respectively between (118.83-147.17mg/mL) of Gallic acid equivalents (mg/mL) ) and (31.33-40.55mg/mL)of BSA (mg/mL), the crude fresh extract and crude protein extract showperoxidase activities, while protease activities were not found in ours extracts. Microbiological tests showed a high antibacterial potential of methanol extracts against tested bacteria ,Enterococcus faecalis (ATCC 29212) wasmost sensitive to methanol extracts (IZD: 17mm to 23.5),in opposite, aqueous extracts show weak antibacterial activities. Crude protein extracts hadnot antibacterial effects while crude fresh extracts were efficiency againstbacterial growth, biochemical enzyme tests revealed that crude extracts ofthe leaves show peroxidase activity wish may to increase antibacterial activities of crude extracts. The high activities of leaf Argan extracts were detected in Oran samples (littoral area) followed by Tindouf samples (arid area),this result confirmed positive relationships between geographical distribution of samples and their antibacterial activities.


2013 ◽  
Vol 826 ◽  
pp. 92-96
Author(s):  
Zheng Xian Ma ◽  
Qiao Ling Liu ◽  
Lan Qin Wang ◽  
Lu Feng Pang

With using the red mud of Bayer process and industrial hydrochloric acid as main initial materials, iron oxide red was prepared by chemical precipitation technique. The character of the sample were characterized and analyzed by means of XRD, SEM, particle size analyzer, and chemical analysis methods, respectively. The optimal experimental condition of extraction of iron from red mud was obtained, and the key quality indicators of sample was conformed the requirements of iron oxide red by a variety of test and characterization.


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