Effect of Calcium and Fluoride Addition to Hydrogen Peroxide Bleaching Gel On Tooth Diffusion, Color, and Microhardness

2019 ◽  
Vol 44 (4) ◽  
pp. 424-432 ◽  
Author(s):  
CRG Torres ◽  
RF Zanatta ◽  
TJ Silva ◽  
AB Borges
Keyword(s):  
Hydrogen Peroxide ◽  
Sodium Fluoride ◽  
Calcium Gluconate ◽  
Color Change ◽  
Artificial Saliva ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Tooth Structure ◽  
Peroxide Bleaching ◽  
Fluoride Addition

SUMMARY Objectives: The aim of this study was to evaluate the effect of calcium and fluoride addition to a 35% hydrogen peroxide (HP) bleaching gel with regard to its diffusion through the tooth structure, enamel microhardness, and bleaching efficacy. Methods and Materials: Eighty specimens (6 mm in diameter and 2 mm in height; 1 mm/enamel and 1 mm/dentin) were obtained from bovine incisors that were polished and divided into four groups (n=20) according to the remineralizing agent added to the gel: Ca = 0.5% calcium gluconate; F = 0.2% sodium fluoride; Ca+F = 0.5% calcium gluconate and 0.2% sodium fluoride; and control = no agent. Initial microhardness and color were assessed. The samples were positioned over simulated pulpal chambers filled with acetate buffer solution to capture the HP. Gels were applied over enamel for 30 minutes, and HP diffusion was assessed by spectrophotometry two hours after bleaching. Microhardness was measured immediately after bleaching and then the specimens were immersed into artificial saliva for seven days for final color assessment. Data were analyzed by one-way analysis of variance followed by Tukey test. Results: Bleaching reduced microhardness for all groups (p=0.0001), but the Ca+F and F groups showed lower reductions after bleaching. The addition of Ca, F, and Ca+F decreased the peroxide penetration through the tooth structure (p=0.0001), but there were no differences in color change for ΔL (p=0.357), Δa (p=0.061), Δb (p=0.823), and ΔE (p=0.581). Conclusion: The addition of calcium and fluoride in the gel did not affect bleaching efficacy, but it was able to reduce both the peroxide diffusion and the bleached enamel microhardness loss.

scholarly journals Penetration of Hydrogen Peroxide and Degradation Rate of Different Bleaching Products

2015 ◽  
Vol 40 (1) ◽  
pp. 72-79 ◽  
Author(s):  
FC Marson ◽  
RS Gonçalves ◽  
CO Silva ◽  
LTÂ Cintra ◽  
RC Pascotto ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Residence Time ◽  
Degradation Rate ◽  
Dental Enamel ◽  
Spectrophotometric Analysis ◽  
Acetate Buffer Solution ◽  
Tooth Surface ◽  
Buffer Solution ◽  
Tooth Structure ◽  
Bleaching Gels

SUMMARY This study's aim was to evaluate the degradation rate of hydrogen peroxide (H2O2) and to quantify its penetration in tooth structure, considering the residence time of bleaching products on the dental enamel. For this study, bovine teeth were randomly divided according to the bleaching product received: Opalescence Xtra Boost 38%, White Gold Office 35%, Whiteness HP Blue 35%, Whiteness HP Maxx 35%, and Lase Peroxide Sensy 35%. To analyze the degradation of H2O2, the titration of bleaching agents with potassium permanganate was used, while the penetration of H2O2 was measured via spectrophotometric analysis of the acetate buffer solution, collected from the artificial pulp chamber. The analyses were performed immediately as well as 15 minutes, 30 minutes, and 45 minutes after product application. The data of degradation rate of H2O2 were submitted to analysis of variance (ANOVA) and Tukey tests, while ANOVA and Fisher tests were used for the quantification of H2O2, at the 5% level. The results showed that all products significantly reduced the concentration of H2O2 activates at the end of 45 minutes. It was also verified that the penetration of H2O2 was enhanced by increasing the residence time of the product on the tooth surface. It was concluded that the bleaching gels retained substantial concentrations of H2O2 after 45 minutes of application, and penetration of H2O2 in the dental structure is time-dependent.

Influence of Hydrogen Peroxide Bleaching Gels on Color, Opacity, and Fluorescence of Composite Resins

2012 ◽  
Vol 37 (5) ◽  
pp. 526-531 ◽  
Author(s):  
CRG Torres ◽  
CF Ribeiro ◽  
E Bresciani ◽  
AB Borges
Keyword(s):  
Hydrogen Peroxide ◽  
Composite Resin ◽  
Color Change ◽  
Composite Resins ◽  
Control Group ◽  
Peroxide Bleaching ◽  
Color Changes ◽  
Tukey Test ◽  
Bleaching Gels ◽  
Bleaching Treatment

SUMMARY The aim of the present study was to evaluate the effect of 20% and 35% hydrogen peroxide bleaching gels on the color, opacity, and fluorescence of composite resins. Seven composite resin brands were tested and 30 specimens, 3-mm in diameter and 2-mm thick, of each material were fabricated, for a total of 210 specimens. The specimens of each tested material were divided into three subgroups (n=10) according to the bleaching therapy tested: 20% hydrogen peroxide gel, 35% hydroxide peroxide gel, and the control group. The baseline color, opacity, and fluorescence were assessed by spectrophotometry. Four 30-minute bleaching gel applications, two hours in total, were performed. The control group did not receive bleaching treatment and was stored in deionized water. Final assessments were performed, and data were analyzed by two-way analysis of variance and Tukey tests (p<0.05). Color changes were significant for different tested bleaching therapies (p<0.0001), with the greatest color change observed for 35% hydrogen peroxide gel. No difference in opacity was detected for all analyzed parameters. Fluorescence changes were influenced by composite resin brand (p<0.0001) and bleaching therapy (p=0.0016) used. No significant differences in fluorescence between different bleaching gel concentrations were detected by Tukey test. The greatest fluorescence alteration was detected on the brand Z350. It was concluded that 35% hydrogen peroxide bleaching gel generated the greatest color change among all evaluated materials. No statistical opacity changes were detected for all tested variables, and significant fluorescence changes were dependent on the material and bleaching therapy, regardless of the gel concentration.

Preparation of porphyrin modified CO9S8 nanocomposites and application for colorimetric biosensing of H2O2

2018 ◽  
Vol 22 (09n10) ◽  
pp. 935-943 ◽  
Author(s):  
Yan Gao ◽  
Chunqiao Jin ◽  
Miaomiao Chen ◽  
Xixi Zhu ◽  
Min Fu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Catalytic Activity ◽  
Peroxidase Activity ◽  
Catalytic Mechanism ◽  
Color Change ◽  
Colorimetric Detection ◽  
Buffer Solution ◽  
Hydrogen Peroxide Detection ◽  
Steady State Kinetics ◽  
One Step

Hydrogen peroxide detection has been widely applied in the fields of biology, medicine, and chemistry. Colorimetric detection of hydrogen peroxide has proven to be a fast and convenient method. In this work, 5,10,15,20-tetrakis(4-chlorophenyl) porphyrin modified Co[Formula: see text]S[Formula: see text] nanocomposites (H[Formula: see text]TClPP-Co[Formula: see text]S[Formula: see text] were prepared via a facile one-step hydrothermal method. H[Formula: see text]TClPP-Co[Formula: see text]S[Formula: see text] nanocomposites were demonstrated to possess an enhanced mimetic peroxidase activity toward the substrate, 3,3[Formula: see text],5,5[Formula: see text]-tetramethylbenzidine (TMB), which can be oxidized to oxTMB (oxidized TMB) in a buffer solution of hydrogen peroxide with a color change from colorless to blue. The catalytic activity of H[Formula: see text]TClPP-Co[Formula: see text]S[Formula: see text] was further analyzed by steady-state kinetics, and H[Formula: see text]TClPP-Co[Formula: see text]S[Formula: see text] had high affinity towards both TMB and H[Formula: see text]O[Formula: see text]. Furthermore, fluorescence and ESR data revealed that the catalytic mechanism of the peroxidase activity of H[Formula: see text]TClPP-Co[Formula: see text]S[Formula: see text] is due to hydroxyl radicals generated from decomposition of H[Formula: see text]O[Formula: see text]. Based on the catalytic activity of H[Formula: see text]TClPP-Co[Formula: see text]S[Formula: see text], a sensitive colorimetric sensor of H[Formula: see text]O[Formula: see text] with a detection limit of 6.803 [Formula: see text]M as well as a range of 7–100 [Formula: see text]M was designed.

scholarly journals Enamel Surface Changes After Exposure to Bleaching Gels Containing Carbamide Peroxide or Hydrogen Peroxide

2016 ◽  
Vol 41 (1) ◽  
pp. E39-E47 ◽  
Author(s):  
B Cvikl ◽  
A Lussi ◽  
A Moritz ◽  
S Flury
Keyword(s):  
Hydrogen Peroxide ◽  
Surface Roughness ◽  
Elastic Modulus ◽  
Color Change ◽  
Surface Hardness ◽  
Enamel Surface ◽  
Carbamide Peroxide ◽  
Peroxide Bleaching ◽  
Bleaching Gels ◽  
Bleaching Treatment

SUMMARY Objective This study evaluated the differences in enamel color change, surface hardness, elastic modulus, and surface roughness between treatments with four bleaching gels containing carbamide peroxide (two at 10% and one each at 35%, and 45%) and two bleaching gels containing hydrogen peroxide (two at 40%). Methods Enamel specimens were bleached and color changes were measured. Color change was calculated using either ΔE or the Bleaching Index (BI). Then, surface hardness, elastic modulus, and surface roughness of the enamel specimens were evaluated. All measurements were performed at baseline and directly after the first bleaching treatment for all carbamide peroxide– and hydrogen peroxide–containing bleaching gels. In addition, final measurements were made 24 hours after each of a total of 10 bleaching treatments for carbamide peroxide bleaching gels, and 1 week after each of a total of three bleaching treatments for hydrogen peroxide bleaching gels. Results After the last bleaching treatment, respective ΔE scores were 17.6 and 8.2 for the two 10% carbamide peroxide gels, 12.9 and 5.6 for the 45% and 35% carbamide peroxide gels, and 9.6 and 13.9 for the two 40% hydrogen peroxide gels. The respective BI scores were −2.0 and −2.0 for the two 10% carbamide peroxide gels, −3.5 and −1.5 for the 45% and 35% carbamide peroxide gels, and −2.0 and −3.0 for the two 40% hydrogen peroxide gels. Each bleaching gel treatment resulted in significant whitening; however, no significant difference was found among the gels after the last bleaching. Whitening occurred within the first bleaching treatments and did not increase significantly during the remaining treatments. Surface hardness significantly decreased after the last bleaching treatment, when 10% carbamide peroxide was used. Furthermore, significant changes in the elastic modulus or surface roughness occurred only after treatment with 10% carbamide peroxide. Conclusion All six bleaching gels effectively bleached the enamel specimens independent of their concentration of peroxide. Gels with low peroxide concentration and longer contact time negatively affected the enamel surface.

scholarly journals Bleaching Gels Containing Calcium and Fluoride: Effect on Enamel Erosion Susceptibility

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Alessandra B. Borges ◽  
Carlos R. G. Torres ◽  
Paulo A. B. de Souza ◽  
Taciana M. F. Caneppele ◽  
Luciana F. T. F. Santos ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Calcium Gluconate ◽  
Artificial Saliva ◽  
Bleaching Agent ◽  
Enamel Erosion ◽  
Agent Control ◽  
Bovine Enamel ◽  
Enamel Wear ◽  
Bleaching Gels

Thisin vitrostudy evaluated the effect of 35% hydrogen peroxide (HP) bleaching gel modified or not by the addition of calcium and fluoride on enamel susceptibility to erosion. Bovine enamel samples (3 mm in diameter) were divided into four groups (n=15) according to the bleaching agent: control—without bleaching (C); 35% hydrogen peroxide (HP); 35% HP with the addition of 2% calcium gluconate (HP + Ca); 35% HP with the addition of 0.6% sodium fluoride (HP + F). The bleaching gels were applied on the enamel surface for 40 min, and the specimens were subjected to erosive challenge with Sprite Zero and remineralization with artificial saliva for 5 days. Enamel wear was assessed using profilometry. The data were analyzed by ANOVA/ Tukey’s test (P<0.05). There were significant differences among the groups (P=0.009). The most enamel wear was seen for C (3.37±0.80 μm), followed by HP (2.89±0.98 μm) and HP + F (2.72±0.64 μm). HP + Ca (2.31±0.92 μm) was the only group able to significantly reduce enamel erosion compared to C. The application of HP bleaching agent did not increase the enamel susceptibility to erosion. However, the addition of calcium gluconate to the HP gel resulted in reduced susceptibility of the enamel to erosion.

Quantification of Peroxide Ion Passage in Dentin, Enamel, and Cementum After Internal Bleaching With Hydrogen Peroxide

2012 ◽  
Vol 37 (6) ◽  
pp. 660-664 ◽  
Author(s):  
RM Palo ◽  
I Bonetti-Filho ◽  
MC Valera ◽  
CHR Camargo ◽  
SEA Camargo ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
External Surface ◽  
Acetate Buffer Solution ◽  
Bleaching Agent ◽  
Pulp Chamber ◽  
Control Groups ◽  
Buffer Solution ◽  
Exposed Area ◽  
Blue Solution ◽  
Nail Varnish

SUMMARY The aim of this study was to evaluate the amount of peroxide passage from the pulp chamber to the external enamel surface during the internal bleaching technique. Fifty bovine teeth were sectioned transversally 5 mm below the cemento-enamel junction (CEJ), and the remaining part of the root was sealed with a 2-mm layer of glass ionomer cement. The external surface of the samples was coated with nail varnish, with the exception of standardized circular areas (6-mm diameter) located on the enamel, exposed dentin, or cementum surface of the tooth. The teeth were divided into three experimental groups according to exposed areas close to the CEJ and into two control groups (n=10/group), as follows: GE, enamel exposure area; GC, cementum exposed area; GD, dentin exposed area; Negative control, no presence of internal bleaching agent and uncoated surface; and Positive control, pulp chamber filled with bleaching agent and external surface totally coated with nail varnish. The pulp chamber was filled with 35% hydrogen peroxide (Opalescence Endo, Ultradent). Each sample was placed inside of individual flasks with 1000 μL of acetate buffer solution, 2 M (pH 4.5). After seven days, the buffer solution was transferred to a glass tube, in which 100 μL of leuco-crystal violet and 50 μL of horseradish peroxidase were added, producing a blue solution. The optical density of the blue solution was determined by spectrophotometer and converted into microgram equivalents of hydrogen peroxide. Data were submitted to Kruskal-Wallis and Dunn-Bonferroni tests (α=0.05). All experimental groups presented passage of peroxide to the external surface that was statistically different from that observed in the control groups. It was verified that the passage of peroxide was higher in GD than in GE (p&lt;0.01). The GC group presented a significantly lower peroxide passage than did GD and GE (p&lt;0.01). It can be concluded that the hydrogen peroxide placed into the pulp chamber passed through the dental hard tissues, reaching the external surface and the periodontal tissue. The cementum surface was less permeable than were the dentin and enamel surfaces.

scholarly journals In vitro tooth whitening effectiveness of whitening mouth rinses

2020 ◽  
Vol 19 ◽  
pp. e206779
Author(s):  
Ana Paula Morona Rodrigues ◽  
Mariele Cristina Klein ◽  
Mauro Antonio Dall Agnol ◽  
Sinval Adalberto Rodrigues-Junior
Keyword(s):  
Hydrogen Peroxide ◽  
Color Change ◽  
Artificial Saliva ◽  
Acrylic Resin ◽  
Mouth Rinse ◽  
Tooth Whitening ◽  
Mouth Rinses ◽  
Immersion Period ◽  
Over Time

Regardless of the extensive availability of mouth rinses that claim to whiten teeth, evidence of achievement of such effect is still missing. Aim: Therefore, this study assessed in vitro the whitening effectiveness of whitening mouth rinses. Methods: Sixty intact bovine incisors were embedded in acrylic resin and had their buccal surface flattened and polished. Then, the specimens were randomly allocated to three conventional (Colgate Plax, Cepacol and Listerine Cool Mint) and three whitening mouth rinse groups (Colgate Luminous White, Cepacol Whitening and Listerine Whitening Extreme) (n=10). Following, the specimens were immersed twice a day in the mouth rinses for one minute for 28 days. In between each immersion period, the specimens remained in artificial saliva at 37oC. Color was measured at baseline, 7, 14, 21, and 28 days using a portable spectrophotometer (Easyshade, Vita Zahnfabrik, Germany) with a 6 mm of diameter probe. Color change was analyzed considering the parameters of ∆L*, ∆a*, ∆b* and, ultimately, ∆E*. The whitening efficacy of the mouth rinses was analyzed using the Whiteness Index for Dentistry (WID). Data of ∆s was analyzed using two-way ANOVA and Tukey’s test (α=0.05). Results: The type of mouth rinse affected significantly all the ∆ parameters (p<0.05). A non-whitening (conventional) mouth rinse produced the highest ΔE*, followed by the three whitening mouth rinses. The application time also affected ΔE* (p<0.05), with emphasis on the third week of treatment. Only the hydrogen peroxide-containing mouth rinse (Listerine Whitening Extreme) presented a whitening effect, with an increasing trend over time. Conclusion: Although the overall color change was not different when comparing conventional and whitening mouth rinses, the hydrogen peroxide-containing whitening mouth rinse produces an increasing whitening trend over time. Not every mouth rinse that claims to whiten teeth produces the desired effect.

scholarly journals Evaluation of surface roughness and color change of bovine enamel after immersion in dye solution

2018 ◽  
Vol 5 (5) ◽  
pp. 95
Author(s):  
Ana Carolina Trentino ◽  
Larissa Marinho Azevedo ◽  
Felipe Fabrício Farias Da Silva ◽  
Maria Cristina Carvalho de Almedra Freitas ◽  
Marina Studart Alencar Borges ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Surface Roughness ◽  
Color Change ◽  
Artificial Saliva ◽  
In Vitro Study ◽  
Black Tea ◽  
Control Group ◽  
Change Analysis ◽  
Miller Test

Aim: The aim of this in vitro study was to evaluate the influence of dye solution on enamel color change after bleaching protocols and the effectiveness in maintaining the color of these agents.Material and Methods: The buccal surfaces of sixty-five bovine incisors were cleaned and polished, and the enamel specimens were divided into thirteen groups: G1 to G6: treated with 6% hydrogen peroxide using different surface agents; G7 to G12: treated with 15% hydrogen peroxide using different surface agents; G13: control. After 24 hours, the groups treated were immersed in black tea solution; the control group was stored in artificial saliva. The color was evaluated prior to bleaching, 24 hours later and after immersion in the dye solution; the roughness was measured immediately after bleaching, 24 hours later and 7 days after immersion in the dye solution. The data was analyzed using the Kruskal-Wallis test, followed by the Miller test for roughness analysis, and the Duncan test for color change analysis. It was used 5% significant level with p<0.05.Results: The data found in the evaluation of surface roughness after bleaching indicated a reduction of roughness in all the groups. The surface agent Bifluoride, when applied, showed an increase in roughness after its application and it decreases after immersion in dye solution; the surface agent Desensibilize and the XP Bond adhesive showed greater color alteration after immersion in dye solution.Conclusions: All the groups studied, under different whitening technique, were effective in promoting whitening.

Effectiveness of In-office Hydrogen Peroxide With Two Different Protocols: A Two-center Randomized Clinical Trial

2018 ◽  
Vol 43 (4) ◽  
pp. 353-361 ◽  
Author(s):  
IEB Martins ◽  
S Onofre ◽  
N Franco ◽  
LM Martins ◽  
A Montenegro ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Color Change ◽  
Absolute Risk ◽  
Rank Test ◽  
Bleaching Agent ◽  
Tooth Sensitivity ◽  
Peroxide Bleaching ◽  
Color Changes ◽  
Ph Values ◽  
The Absolute

SUMMARY Objectives: The aim of this study was to compare the bleaching efficacy and tooth sensitivity (TS) of a 38% hydrogen peroxide bleaching agent used for in-office bleaching, applied under different time protocols: a 40-minute application or two 20-minute applications. Methods and Materials: Forty-four patients from Brazil and Colombia, with right superior canines darker than C2, were selected for this multicenter, single-blind, randomized trial. The teeth were bleached in two sessions, with a one-week interval between them, in a split-mouth design. The bleaching agent was applied in two 20-minute (2×20) applications or one 40-minute (1×40) application in each session according to the manufacturer's instructions. The color changes were evaluated by using subjective (Vita Classical and Vita Bleachedguide) and objective (Easyshade Spectrophotometer) methods at baseline and 30 days after the second session. Tooth sensitivity was recorded up to 48 hours with a 0-10 visual analog scale. Also, the pH values during the application of bleaching were recorded. Color change in shade guide units and ΔE were analyzed by using the Student t-test (α=0.05). The absolute risk and intensity of TS were evaluated with the McNemar test, the Wilcoxon signed-rank test, and the Friedman test, respectively (α= 0.05). Results: Significant whitening was observed in both groups after 30 days of clinical evaluation. The use of a 40-minute application did not significantly influence the absolute risk of TS (68%, 95% confidence interval [CI] = 53-80) as well as the intensity of TS compared with the acid bleaching gel (absolute risk of 82%, 95% CI = 68-91). The pH values did not differ significantly between groups and at the different assessment periods (p=0.42). Conclusion: The use of a 40-minute in-office bleaching agent gel application produced the same whitening degree and TS that the two 20-minute bleaching agent applications did. The former preferably should be applied because one 40-minute application does not require gel refreshing.

scholarly journals Influence of Time on Bond Strength After Bleaching with 35% Hydrogen Peroxide

2008 ◽  
Vol 9 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Flávia Martão Flório ◽  
Cinthia Maria Barbosa ◽  
Robson Tetsuo Sasaki ◽  
Robera Tarkany Basting
Keyword(s):  
Hydrogen Peroxide ◽  
Bond Strength ◽  
Composite Resin ◽  
Artificial Saliva ◽  
Testing Machine ◽  
Adhesive System ◽  
Bleaching Agent ◽  
Peroxide Bleaching ◽  
Bleaching Treatment

Abstract Aim The aim of this in vitro study was to evaluate the influence of time after treatment with a 35% hydrogen peroxide bleaching agent on the shear bond strength between composite resin and sound enamel and dentin. Methods and Materials Eighty dental slabs - 40 enamel (E) slabs and 40 dentin (D) slabs - were embedded, flatted, and divided into four groups (n=10). In G1 the E and D slabs were kept in artificial saliva for 14 days. For the G2, G3, and G4 groups the E and D slabs were submitted to bleaching treatment with a 35% hydrogen peroxide bleaching agent. At different times after bleaching treatments (G2=immediate; G3=seven days; G4= fourteen days), composite resin cylinders were made using an adhesive system. Tests were performed in a universal testing machine at a speed of 0.5 mm/min to obtain the values in MPa. Results For enamel, the Kruskal-Wallis test and Dunn Method showed G1 differed significantly from G2 (G1=13.40 a; G2=6.64 b; G3=16.76 a; G4=11.64 ab). For dentin, the analysis of variance (ANOVA) and Tukey tests showed that G1 differed significantly from G2 and G3 (G1=12.11 a; G2=4.97 b; G3=8.67 c; G4=11.86 ac). Conclusion It is recommended adhesive restorative procedures in enamel be delayed for seven days postbleaching treatment with 35% hydrogen peroxide, while restorations in dentin should be delayed for 14 days following bleaching treatment. Citation Barbosa CM, Sasaki RT, Flório FM, Basting RT. Influence of Time on Bond Strength After Bleaching with 35% Hydrogen Peroxide. J Contemp Dent Pract 2008 February;(9)2:081-088.

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