Cytotoxic Effects of Hydrogen Peroxide on Human Gingival Fibroblasts In Vitro

2015 ◽  
Vol 40 (4) ◽  
pp. 430-439 ◽  
Author(s):  
M Furukawa ◽  
Jr K-Kaneyama ◽  
M Yamada ◽  
A Senda ◽  
A Manabe ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Vitamin E ◽  
Survival Rates ◽  
Human Gingival Fibroblasts ◽  
Toxic Effects ◽  
Bleaching Agent ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects ◽  
Peripheral Tissues ◽  
Tnf Α

SUMMARY In-office bleaching is a popular treatment in modern esthetic dentistry. However, bleaching agents sometimes accidentally adhere to the gingiva and peripheral tissues, even when applied by well-trained dentists. This can lead to transient pain and whitish changes in the gingiva. Although these symptoms disappear within several hours, the effects of bleaching agents on gingiva have not been well described in the literature. The present study aimed to elucidate the cytotoxic effects of a bleaching agent on cultured human gingival fibroblasts (HGFs). We performed a comprehensive analysis of the toxic effects of in-office bleaching agents on gingiva using cultured HGFs and DNA microarray. Survival rates of HGFs decreased with increases in the concentration of hydrogen peroxide, which became significant at concentrations of 1.5 × 10−3% or higher at every time point. Concentrations lower than 1.5 × 10−3% did not affect survival rates of HGFs. Cytotoxicity of hydrogen peroxide was significantly weakened by the addition of vitamin E. Stimulation by in-office bleaching agents triggered the proinflammatory cytokine tumor necrosis factor (TNF)–α cascade in gingival fibroblasts. As the TNF-α cascade can be inhibited by vitamin E additives, treatment with vitamin E may protect gingival fibroblasts against the toxic effects of an in-office bleaching agent. The present results suggest that local administration of vitamin E to gingiva before in-office bleaching may be useful for preventing gingival irritation due to accidental adhesion of a bleaching agent.

scholarly journals Factors Contributing to Hydrogen Peroxide Resistance in Streptococcus pneumoniae Include Pyruvate Oxidase (SpxB) and Avoidance of the Toxic Effects of the Fenton Reaction

2003 ◽  
Vol 185 (23) ◽  
pp. 6815-6825 ◽  
Author(s):  
Christopher D. Pericone ◽  
Sunny Park ◽  
James A. Imlay ◽  
Jeffrey N. Weiser
Keyword(s):  
Hydrogen Peroxide ◽  
Streptococcus Pneumoniae ◽  
Fenton Reaction ◽  
Bacterial Species ◽  
Atp Depletion ◽  
Host Cells ◽  
Toxic Effects ◽  
Aerobic Growth ◽  
Pyruvate Oxidase ◽  
Cytotoxic Effects

ABSTRACT Aerobic growth of Streptococcus pneumoniae results in production of amounts of hydrogen peroxide (H2O2) that may exceed 1 mM in the surrounding media. H2O2 production by S. pneumoniae has been shown to kill or inhibit the growth of other respiratory tract flora, as well as to have cytotoxic effects on host cells and tissue. The mechanisms allowing S. pneumoniae, a catalase-deficient species, to survive endogenously generated concentrations of H2O2 that are sufficient to kill other bacterial species is unknown. In the present study, pyruvate oxidase (SpxB), the enzyme responsible for endogenous H2O2 production, was required for survival during exposure to high levels (20 mM) of exogenously added H2O2. Pretreatment with H2O2 did not increase H2O2 resistance in the mutant, suggesting that SpxB activity itself is required, rather than an H2O2-inducible pathway. SpxB mutants synthesized 85% less acetyl-phosphate, a potential source of ATP. During H2O2 exposure, ATP levels decreased more rapidly in spxB mutants than in wild-type cells, suggesting that the increased killing of spxB mutants was due to more rapid ATP depletion. Together, these data support the hypothesis that S. pneumoniae SpxB contributes to an H2O2-resistant energy source that maintains viability during oxidative stress. Thus, SpxB is required for resistance to the toxic by-product of its own activity. Although H2O2-dependent hydroxyl radical production and the intracellular concentration of free iron were similar to that of Escherichia coli, killing by H2O2 was unaffected by iron chelators, suggesting that S. pneumoniae has a novel mechanism to avoid the toxic effects of the Fenton reaction.

Activation of ERK1/2 by protein kinase C-α in response to hydrogen peroxide-induced cell death in human gingival fibroblasts

2010 ◽  
Vol 24 (1) ◽  
pp. 319-326 ◽  
Author(s):  
Gloria Gutiérrez-Venegas ◽  
Juan Antonio Arreguín-Cano ◽  
Rita Arroyo-Cruz ◽  
Mónica Villeda-Navarro ◽  
José Antonio Méndez-Mejía
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Death ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Kinase C

scholarly journals Chrysin and rutin protect against hydrogen peroxide and tert-butyl hydroperoxide induced oxidative cell damage

2021 ◽  
Vol 11 (6) ◽  
pp. 20-25
Author(s):  
Rouamba Ablassé ◽  
Compaoré Moussa ◽  
Ouédraogo Maurice ◽  
Bationo Raoul ◽  
Kiendrebeogo Martin
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Damage ◽  
Human Gingival Fibroblasts ◽  
Dependent Manner ◽  
Gingival Fibroblasts ◽  
Cytoprotective Effect ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Thiazolyl Blue Tetrazolium Bromide ◽  
Tert Butyl Hydroperoxide

Objective: Chrysin and rutin are two dietary flavonoids lying in fruits or honey bee’s products. Their pharmacological properties include antioxidant, anti-inflammatory, anticancer, neuroprotection and immunomodulatory. In the current study, the potentiality of chrysin and rutin to protect human gingival fibroblasts against oxidative cell damage has been investigated in vitro.   Method: Human gingival fibroblasts, passage 3, were concomitantly put in contact with the cytotoxic compounds and chrysin or rutin for 24 h at 37 °C, 5% CO2 atmosphere, and 96% humidity. The amount of viable cell after the incubated time was recorded by using the thiazolyl blue tetrazolium bromide (MTT) assay.  Results: Chrysin in all tested concentration didn’t exhibit any cytoprotective effect against the tert-butyl hydroperoxide-induced oxidative cell damage. Moreover, chrysin in a low concentration (5 and 10 µg/mL) didn’t protect the fibroblasts against oxidative cell damage induced by the hydrogen peroxide. However, chrysin in a concentration of 20 µg/mL showed a significant cytoprotective activity in the hydrogen peroxide-induced cell damage (p < 0.05). Rutin in all tested concentrations protected fibroblasts against hydrogen peroxide and tert-butyl hydroperoxide-induced oxidative cell damage. The cytoprotective effect of rutin didn’t increase with the increase of the concentration when hydrogen peroxide is used to induce oxidative cell damage. However, rutin has protected cells against the tert-butyl hydroperoxide cytotoxicity in a concentration dependent manner. Conclusion: Given to the interesting cytoprotective activities exhibited by chrysin and rutin, further investigations to highlight their cytoprotective involved mechanisms are justified.   Keywords: Chrysin, Cytoprotective, Fibroblasts, Rutin.

scholarly journals Expression of deubiquitinases in human gingiva and cultured human gingival fibroblasts

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yong-Wei Fu ◽  
Hong-Zhi Xu
Keyword(s):  
Protein Expression ◽  
Human Gingival Fibroblasts ◽  
Gingival Tissue ◽  
Gingival Fibroblasts ◽  
Western Blots ◽  
Tissue Samples ◽  
Periodontal Tissues ◽  
Tnf Α ◽  
Gene And Protein Expression ◽  
Human Gingiva

Abstract Background Although deubiquitinating enzymes (DUBs) such as CYLD, A20 and OTULIN are expressed in multiple tissues and thought to be linked with inflammatory diseases, their expression in periodontal tissues remains to be determined. This research was designed to assess the expression of CYLD, A20 and OTULIN in human gingiva, and to evaluate the regulation of these DUBs in human gingival fibroblasts (HGFs) upon different stimuli. Methods Immunohistochemistry assay was conducted to determine the expression of CYLD, A20 and OTULIN in human gingiva. Immunofluorescence assay was employed to observe the protein expression of CYLD, A20 and OTULIN in HGFs. RT-PCR and western blots were carried out to assess gene and protein expression changes of these DUBs in HGFs upon LPS or TNF-α. Results CYLD, A20 and OTULIN were found to be expressed in human gingiva and HGFs. The expression of CYLD, A20 and OTULIN was lower in the inflamed gingival tissue samples compared with the healthy gingival tissue samples. Further, the expression of CYLD, A20 and OTULIN in HGFs exhibited distinct regulation by different stimuli. TNF-α treatment markedly increased NF-κB activation in HGFs Conclusions Our findings suggest that CYLD, A20 and OTULIN might play a role in the progression of periodontitis.

The Direct Cytotoxic Effects of Different Hemostatic Agents on Human Gingival Fibroblasts

2018 ◽  
Vol 28 (4) ◽  
pp. e896-e901 ◽  
Author(s):  
Nawaf Labban ◽  
Hanan AlOtaibi ◽  
Abdullah Mokeem ◽  
Mohammad AlJameel ◽  
Talal AlRasheed ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects ◽  
Hemostatic Agents

Cytotoxic effects of gingival retraction cords on human gingival fibroblasts in vitro

2004 ◽  
Vol 31 (4) ◽  
pp. 368-372 ◽  
Author(s):  
C.-M. Liu ◽  
F.-M. Huang ◽  
L.-C. Yang ◽  
L. S.-S. Chou ◽  
M.-Y. Chou ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects

scholarly journals In Vitro Determination of Genotoxicity Induced by Brackets Alloys in Cultures of Human Gingival Fibroblasts

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Juan Pablo Loyola-Rodríguez ◽  
Ildelfonso Lastra-Corso ◽  
José Obed García-Cortés ◽  
Alejandra Loyola-Leyva ◽  
Rúben Abraham Domínguez-Pérez ◽  
...  
Keyword(s):  
Comet Assay ◽  
Aluminum Oxide ◽  
Study Groups ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cobalt Chromium ◽  
Cytotoxic Effects ◽  
Significant Difference

Orthodontic brackets release ions that can be reabsorbed in the oral mucosa, potentially causing complications, including cytotoxic effects and mutagenic alterations. The aim was to evaluate the genotoxicity induced by orthodontic appliance alloys in cultures of human gingival fibroblasts by comet assay. Eluates were obtained from the following brackets alloys: EconoLine (SS: stainless steel), MiniMirage (Ni-Ti: nickel-titanium), Nu-Edge (Co-Cr: cobalt-chromium), In-Vu (PC-polycrystals (PC) aluminum oxide), and Monocrystal IZE (monocrystalline (MC) aluminum oxide). Each bracket was sterilized and exposed to a corrosive process for 35 days. The obtained eluates were tested for genotoxicity of human gingival fibroblasts (HGFA) by the alkaline comet assay. All study groups showed genotoxic effects; there was a significant difference (p<0.0001) among groups. The eluates obtained from Ni-Ti showed a 16-times greater genotoxic effect. There were differences in genotoxicity after comparing the Ni-Ti with SS (p<0.01) and Co-Cr brackets (p<0.001). The ceramic was more genotoxic than metallic brackets (SS and Co-Cr), but less than the Ni-Ti. This in vitro model will be useful for further study of early DNA damage caused by brackets and other biomaterials used in the oral cavity before their introduction into the clinical setting.

scholarly journals Cytotoxicity of the Ingredients of Commonly Used Toothpastes and Mouthwashes on Human Gingival Fibroblasts

2020 ◽  
Author(s):  
Masoumeh Hasani Tabatabaei ◽  
Farzaneh Sadeghi Mahounak ◽  
Nafiseh Asgari ◽  
Zohreh Moradi
Keyword(s):  
Mtt Assay ◽  
Sodium Benzoate ◽  
Sodium Lauryl Sulfate ◽  
Human Gingival Fibroblasts ◽  
Lauryl Sulfate ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects ◽  
Mucosal Tissues ◽  
The Difference ◽  
Cocamidopropyl Betaine

Objectives: Toothpastes and mouthwashes contain ingredients that may be toxic for oral mucosal tissues. This study aimed to assess the cytotoxicity of the ingredients of commonly used toothpastes and mouthwashes. Materials and Methods: This experimental study was performed on 16 toothpastes and four mouthwashes widely available in the Iranian market. First, the concentration of six main ingredients of these products, namely sodium fluoride (NaF), sodium lauryl sulfate, cocamidopropyl betaine, zinc lactate, paraben, and sodium benzoate, was determined. The methyl thiazolyl tetrazolium (MTT) assay was used to assess the cytotoxicity of these materials for human gingival fibroblasts (HGFs). The MTT assay was performed at 1, 15, and 30 minutes following exposure to five concentrations of each material in triplicate (according to the concentrations obtained in the isolation step). Data were analyzed using three-way analysis of variance (ANOVA). Results: The difference in the cytotoxicity of the materials was statistically significant (P<0.001). Cytotoxicity was time- and concentration-dependent; by an increase in the concentration of the materials, their cytotoxicity increased over time. The cytotoxicity of sodium lauryl sulfate and cocamidopropyl betaine was >90%. The cytotoxicity of NaF varied from 25% to 70%, and the cytotoxicity of all concentrations of zinc lactate and sodium benzoate was <50% for HGFs. Conclusion: To decrease the cytotoxic effects of toothpastes, sodium lauryl sulfate and cocamidopropyl betaine should be replaced with safer detergents, and the concentration of fluoride should be decreased to 400 parts per million (ppm). Alternatively, fluoride may be replaced with other antibacterial and cariostatic agents.

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