scholarly journals In Vitro Determination of Genotoxicity Induced by Brackets Alloys in Cultures of Human Gingival Fibroblasts

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Juan Pablo Loyola-Rodríguez ◽  
Ildelfonso Lastra-Corso ◽  
José Obed García-Cortés ◽  
Alejandra Loyola-Leyva ◽  
Rúben Abraham Domínguez-Pérez ◽  
...  
Keyword(s):  
Comet Assay ◽  
Aluminum Oxide ◽  
Study Groups ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cobalt Chromium ◽  
Cytotoxic Effects ◽  
Significant Difference

Orthodontic brackets release ions that can be reabsorbed in the oral mucosa, potentially causing complications, including cytotoxic effects and mutagenic alterations. The aim was to evaluate the genotoxicity induced by orthodontic appliance alloys in cultures of human gingival fibroblasts by comet assay. Eluates were obtained from the following brackets alloys: EconoLine (SS: stainless steel), MiniMirage (Ni-Ti: nickel-titanium), Nu-Edge (Co-Cr: cobalt-chromium), In-Vu (PC-polycrystals (PC) aluminum oxide), and Monocrystal IZE (monocrystalline (MC) aluminum oxide). Each bracket was sterilized and exposed to a corrosive process for 35 days. The obtained eluates were tested for genotoxicity of human gingival fibroblasts (HGFA) by the alkaline comet assay. All study groups showed genotoxic effects; there was a significant difference (p<0.0001) among groups. The eluates obtained from Ni-Ti showed a 16-times greater genotoxic effect. There were differences in genotoxicity after comparing the Ni-Ti with SS (p<0.01) and Co-Cr brackets (p<0.001). The ceramic was more genotoxic than metallic brackets (SS and Co-Cr), but less than the Ni-Ti. This in vitro model will be useful for further study of early DNA damage caused by brackets and other biomaterials used in the oral cavity before their introduction into the clinical setting.

scholarly journals Corrigendum to “In Vitro Determination of Genotoxicity Induced by Brackets Alloys in Cultures of Human Gingival Fibroblasts”

2021 ◽  
Vol 2021 ◽  
pp. 1-1
Author(s):  
Juan Pablo Loyola-Rodríguez ◽  
Ildelfonso Lastra-Corso ◽  
José Obed García-Cortés ◽  
Alejandra Loyola-Leyva ◽  
Rúben Abraham Domínguez-Pérez ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

scholarly journals In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials

2016 ◽  
Vol 17 (6) ◽  
pp. 457-462 ◽  
Author(s):  
Isleine P Caldas ◽  
Miriam Z Scelza ◽  
Marco A Gallito ◽  
Gutemberg Alves ◽  
Licínio Silva
Keyword(s):  
Cell Viability ◽  
Human Fibroblasts ◽  
Primary Cultures ◽  
Polystyrene Latex ◽  
Test Method ◽  
Human Gingival Fibroblasts ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Significant Difference

ABSTRACT Aims The aim of this study is to evaluate the in vitro response of human gingival fibroblasts in primary cultures to two materials for temporary relining of dentures: Temporary Soft (TDV, Brazil) and Trusoft (Bosworth, USA) for 24 hours, 7 and 30 days by using a multi-parametric analysis. Materials and methods Each material sample (TDV, TS, Polystyrene, Latex) was prepared and incubated in a culture medium for 1, 7, and 30 days at 37°C. Human gingival fibroblasts were exposed to the extracts and cell viability was evaluated by a multi-parametric assay, which allowed sequential analysis of mitochondrial activity (XTT), membrane integrity [neutral red (NR)], and cell density [crystal violet dye exclusion (CVDE)] in the same cells. Analysis of variance (ANOVA) was used to test the interactions of the three sources of variation (material, test method, and time) with the proportions of viable cells for each relining material. Results Both evaluated materials (TDV and TS) had low cytotoxic effects during 1, 7, and 30 days after manipulation of the material, as assessed by all three methods used. A statistical difference was found when comparing the negative control group (latex fragments) with the other groups, which showed high toxicity and low percentage of cell viability in all tests used. There was no significant difference among other materials (p > 0.05). Conclusion Low cytotoxicity levels were detected by representatives of the major groups of temporary prosthetic relining materials, as evaluated by multiple cellular viability parameters in human fibroblasts. Clinical significance There are various soft materials on the market for relining prostheses; however, the effects of these materials on tissues need to be clarified to avoid problems for patients. How to cite this article Caldas IP, Scelza MZ, Gallito MA, Alves G, Silva L. In vitro Analysis of Cytotoxicity of Temporary Resilient Relining Materials. J Contemp Dent Pract 2016;17(6):457-462.

Cytotoxic effects of gingival retraction cords on human gingival fibroblasts in vitro

2004 ◽  
Vol 31 (4) ◽  
pp. 368-372 ◽  
Author(s):  
C.-M. Liu ◽  
F.-M. Huang ◽  
L.-C. Yang ◽  
L. S.-S. Chou ◽  
M.-Y. Chou ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects

Mouthrinses with Alcohol: Cytotoxic Effects on Human Gingival Fibroblasts In Vitro

2003 ◽  
Vol 74 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Paola Poggi ◽  
Ruggero Rodriguez Y. Baena ◽  
Silvana Rizzo ◽  
Massimo T. Rota
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects

scholarly journals Cellular effects of glycine and trehalose air-polishing powders on human gingival fibroblasts in vitro

2021 ◽  
Author(s):  
Jens Weusmann ◽  
James Deschner ◽  
Jean-Claude Imber ◽  
Anna Damanaki ◽  
Natalia D. P. Leguizamón ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Function ◽  
Nuclear Translocation ◽  
In Vitro Study ◽  
Human Gingival Fibroblasts ◽  
Mrna Levels ◽  
Gingival Fibroblasts ◽  
Air Polishing ◽  
Wide Range

Abstract Objectives Air-polishing has been used in the treatment of periodontitis and gingivitis for years. The introduction of low-abrasive powders has enabled the use of air-polishing devices for subgingival therapy. Within the last decade, a wide range of different low-abrasive powders for subgingival use has been established. In this study, the effects of a glycine powder and a trehalose powder on human gingival fibroblasts (HGF) were investigated. Methods HGF were derived from three systemically and periodontally healthy donors. After 24 h and 48 h of incubation time, mRNA levels, and after 48 h, protein levels of TNFα, IL-8, CCL2, and VEGF were determined. In addition, NF-κB p65 nuclear translocation and in vitro wound healing were assessed. Statistical analysis was performed by ANOVA and post hoc Dunnett’s and Tukey’s tests (p < 0.05). Results Glycine powder significantly increased the expression of proinflammatory genes and showed exploitation of the NF-κB pathway, albeit trehalose powder hardly interfered with cell function and did not trigger the NF-κB pathway. In contrast to trehalose, glycine showed a significant inhibitory effect on the in vitro wound healing rate. Conclusion Subgingivally applicable powders for air-polishing devices can regulate cell viability and proliferation as well as cytokine expression. Our in vitro study suggests that the above powders may influence HGF via direct cell effects. Trehalose appears to be relatively inert compared to glycine powder.

Azithromycin suppresses P. gingivalis LPS-induced pro-inflammatory cytokine and chemokine production by human gingival fibroblasts in vitro

2014 ◽  
Vol 19 (2) ◽  
pp. 221-227 ◽  
Author(s):  
C. J. Doyle ◽  
T. R. Fitzsimmons ◽  
C. Marchant ◽  
A. A. S. S. K. Dharmapatni ◽  
R. Hirsch ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Chemokine Production

In Vitro Attachment of Human Gingival Fibroblasts to Root Surfaces

1975 ◽  
Vol 46 (11) ◽  
pp. 639-645 ◽  
Author(s):  
Joseph J. Aleo ◽  
Frank A. De Renzis ◽  
Paul A. Farber
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Root Surfaces
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