The Direct Cytotoxic Effects of Different Hemostatic Agents on Human Gingival Fibroblasts

2018 ◽  
Vol 28 (4) ◽  
pp. e896-e901 ◽  
Author(s):  
Nawaf Labban ◽  
Hanan AlOtaibi ◽  
Abdullah Mokeem ◽  
Mohammad AlJameel ◽  
Talal AlRasheed ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects ◽  
Hemostatic Agents

Cytotoxic effects of gingival retraction cords on human gingival fibroblasts in vitro

2004 ◽  
Vol 31 (4) ◽  
pp. 368-372 ◽  
Author(s):  
C.-M. Liu ◽  
F.-M. Huang ◽  
L.-C. Yang ◽  
L. S.-S. Chou ◽  
M.-Y. Chou ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects

scholarly journals In Vitro Determination of Genotoxicity Induced by Brackets Alloys in Cultures of Human Gingival Fibroblasts

2020 ◽  
Vol 2020 ◽  
pp. 1-6 ◽  
Author(s):  
Juan Pablo Loyola-Rodríguez ◽  
Ildelfonso Lastra-Corso ◽  
José Obed García-Cortés ◽  
Alejandra Loyola-Leyva ◽  
Rúben Abraham Domínguez-Pérez ◽  
...  
Keyword(s):  
Comet Assay ◽  
Aluminum Oxide ◽  
Study Groups ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cobalt Chromium ◽  
Cytotoxic Effects ◽  
Significant Difference

Orthodontic brackets release ions that can be reabsorbed in the oral mucosa, potentially causing complications, including cytotoxic effects and mutagenic alterations. The aim was to evaluate the genotoxicity induced by orthodontic appliance alloys in cultures of human gingival fibroblasts by comet assay. Eluates were obtained from the following brackets alloys: EconoLine (SS: stainless steel), MiniMirage (Ni-Ti: nickel-titanium), Nu-Edge (Co-Cr: cobalt-chromium), In-Vu (PC-polycrystals (PC) aluminum oxide), and Monocrystal IZE (monocrystalline (MC) aluminum oxide). Each bracket was sterilized and exposed to a corrosive process for 35 days. The obtained eluates were tested for genotoxicity of human gingival fibroblasts (HGFA) by the alkaline comet assay. All study groups showed genotoxic effects; there was a significant difference (p<0.0001) among groups. The eluates obtained from Ni-Ti showed a 16-times greater genotoxic effect. There were differences in genotoxicity after comparing the Ni-Ti with SS (p<0.01) and Co-Cr brackets (p<0.001). The ceramic was more genotoxic than metallic brackets (SS and Co-Cr), but less than the Ni-Ti. This in vitro model will be useful for further study of early DNA damage caused by brackets and other biomaterials used in the oral cavity before their introduction into the clinical setting.

Cytotoxic Effects of Hydrogen Peroxide on Human Gingival Fibroblasts In Vitro

2015 ◽  
Vol 40 (4) ◽  
pp. 430-439 ◽  
Author(s):  
M Furukawa ◽  
Jr K-Kaneyama ◽  
M Yamada ◽  
A Senda ◽  
A Manabe ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Vitamin E ◽  
Survival Rates ◽  
Human Gingival Fibroblasts ◽  
Toxic Effects ◽  
Bleaching Agent ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects ◽  
Peripheral Tissues ◽  
Tnf Α

SUMMARY In-office bleaching is a popular treatment in modern esthetic dentistry. However, bleaching agents sometimes accidentally adhere to the gingiva and peripheral tissues, even when applied by well-trained dentists. This can lead to transient pain and whitish changes in the gingiva. Although these symptoms disappear within several hours, the effects of bleaching agents on gingiva have not been well described in the literature. The present study aimed to elucidate the cytotoxic effects of a bleaching agent on cultured human gingival fibroblasts (HGFs). We performed a comprehensive analysis of the toxic effects of in-office bleaching agents on gingiva using cultured HGFs and DNA microarray. Survival rates of HGFs decreased with increases in the concentration of hydrogen peroxide, which became significant at concentrations of 1.5 × 10−3% or higher at every time point. Concentrations lower than 1.5 × 10−3% did not affect survival rates of HGFs. Cytotoxicity of hydrogen peroxide was significantly weakened by the addition of vitamin E. Stimulation by in-office bleaching agents triggered the proinflammatory cytokine tumor necrosis factor (TNF)–α cascade in gingival fibroblasts. As the TNF-α cascade can be inhibited by vitamin E additives, treatment with vitamin E may protect gingival fibroblasts against the toxic effects of an in-office bleaching agent. The present results suggest that local administration of vitamin E to gingiva before in-office bleaching may be useful for preventing gingival irritation due to accidental adhesion of a bleaching agent.

scholarly journals Cytotoxicity of the Ingredients of Commonly Used Toothpastes and Mouthwashes on Human Gingival Fibroblasts

2020 ◽  
Author(s):  
Masoumeh Hasani Tabatabaei ◽  
Farzaneh Sadeghi Mahounak ◽  
Nafiseh Asgari ◽  
Zohreh Moradi
Keyword(s):  
Mtt Assay ◽  
Sodium Benzoate ◽  
Sodium Lauryl Sulfate ◽  
Human Gingival Fibroblasts ◽  
Lauryl Sulfate ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects ◽  
Mucosal Tissues ◽  
The Difference ◽  
Cocamidopropyl Betaine

Objectives: Toothpastes and mouthwashes contain ingredients that may be toxic for oral mucosal tissues. This study aimed to assess the cytotoxicity of the ingredients of commonly used toothpastes and mouthwashes. Materials and Methods: This experimental study was performed on 16 toothpastes and four mouthwashes widely available in the Iranian market. First, the concentration of six main ingredients of these products, namely sodium fluoride (NaF), sodium lauryl sulfate, cocamidopropyl betaine, zinc lactate, paraben, and sodium benzoate, was determined. The methyl thiazolyl tetrazolium (MTT) assay was used to assess the cytotoxicity of these materials for human gingival fibroblasts (HGFs). The MTT assay was performed at 1, 15, and 30 minutes following exposure to five concentrations of each material in triplicate (according to the concentrations obtained in the isolation step). Data were analyzed using three-way analysis of variance (ANOVA). Results: The difference in the cytotoxicity of the materials was statistically significant (P<0.001). Cytotoxicity was time- and concentration-dependent; by an increase in the concentration of the materials, their cytotoxicity increased over time. The cytotoxicity of sodium lauryl sulfate and cocamidopropyl betaine was >90%. The cytotoxicity of NaF varied from 25% to 70%, and the cytotoxicity of all concentrations of zinc lactate and sodium benzoate was <50% for HGFs. Conclusion: To decrease the cytotoxic effects of toothpastes, sodium lauryl sulfate and cocamidopropyl betaine should be replaced with safer detergents, and the concentration of fluoride should be decreased to 400 parts per million (ppm). Alternatively, fluoride may be replaced with other antibacterial and cariostatic agents.

Mouthrinses with Alcohol: Cytotoxic Effects on Human Gingival Fibroblasts In Vitro

2003 ◽  
Vol 74 (5) ◽  
pp. 623-629 ◽  
Author(s):  
Paola Poggi ◽  
Ruggero Rodriguez Y. Baena ◽  
Silvana Rizzo ◽  
Massimo T. Rota
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects

Cytotoxic effects to mouse and human gingival fibroblasts of a nanohybrid ormocer versus dimethacrylate-based composites

2018 ◽  
Vol 23 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Andrea Schubert ◽  
Christopher Ziegler ◽  
Andrea Bernhard ◽  
Ralf Bürgers ◽  
Nicolai Miosge
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Cytotoxic Effects

scholarly journals Effect of Mouthrinse Containing Propolis on Oral Microorganisms and Human Gingival Fibroblasts

2007 ◽  
Vol 01 (04) ◽  
pp. 195-201 ◽  
Author(s):  
Fatih Özan ◽  
Zeynep Sümer ◽  
Zübeyde Akın Polat ◽  
Kürşat Er ◽  
Ülkü Özan ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Propylene Glycol ◽  
Human Gingival Fibroblasts ◽  
Control Group ◽  
Gingival Fibroblasts ◽  
Antibacterial Effects ◽  
Diffusion Test ◽  
Cytotoxic Effects ◽  
Agar Diffusion Test ◽  
Oral Microorganisms

ABSTRACTObjectives: The aim of this study was to compare the effects of four different mouthrinse containing propolis solutions and mouthrinse containing 0.2% chlorhexidine (CHX) on oral microorganisms and human gingival fibroblasts.Methods: Four different solutions of propolis were prepared and propylene glycol and alcohol were used as solvents for each propolis sample. Mouthrinse containing propolis was prepared at four different concentrations as 10%, 5%, 2.5% and 1%. Besides, CHX was used as control group. The antibacterial effects of five solutions on oral microorganisms were tested and their cytotoxic effects on human gingival fibroblasts were evaluated by agar diffusion test.Results: At this concentrations effectiveness of mouthrinse containing propolis samples on oral microorganisms were not found as effective as CHX. On the contrary, samples found less cytotoxic on human gingival fibroblasts than CHX.Conclusions: Standardized preparations of propolis can be used as a mouthrinse at appropriate concentrations. To obtain a standardized chemical composition, advanced researches are needed. (Eur J Dent 2007;1:195-201)

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